检索结果-南通市图书馆

“Love Is Strong, and you＇re so Sweet”： JAGGER Is Essential for Persistent Synergid degeneration and Polytubey Block in Arabidopsis thaliana

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

molecular Plant 2016年第4期9卷 601-614页

作者： Ana Marta Pereira Margarida Sofia Nobre Sara Cristina Pinto Ana Lucia Lopes Mario Luis Costa Simona Masiero Silvia Coimbra departamento de biologia Faculdade de Ciencias da Universidade do Porto Rua do Campo Alegre 4169-007 Porto Portugal Plant Functional Genomics Group Biosystems and Integrative Sciences Institute (BiolSI) 4169-007 Porto Portugal Dipartimento di BioScienze Universita degli Studi di Milano 20133 Milan Italy present address: Institute of Plant Biology and ZUrich-Basel Plant Science Center University of ZUrich Zolikerstrasse 107 8008 Zurich Switzerland

Successful double fertilization and subsequent seed development in flowering plants requires the delivery of two sperm cells, transported by a pollen tube, into the embryo sac of an ovule. The embryo sac cells tightly control synergid cell death, and as a result the polyspermy block. Arabinogalactan proteins are highly glycosylated proteins thought to be involved in several steps of the reproductive process. We show that JAGGER, Arabinogalactan Protein 4, is an important molecule necessary to prevent the growth of multiple pollen tubes into one embryo sac in Arabidopsis thaliana. In jagger, an AGP4 knockout mutant, the pistils show impaired pollen tube blockage as a consequence of the survival of the persistent synergid. JAGGER seems to be involved in the signaling pathway that leads to a blockage of pollen tube attraction. Our results shed light on the mechanism responsible for preventing polyspermy in Arabidopsis and for safe- guarding successful fertilization of all ovules in one pistil, ensuring seed set and the next generation.

关键词： Polytubey persistent synergid arabinogalactan proteins polyspermy

Comparative Transcriptomic Analysis of Salt Adaptation in Roots of Contrasting Medicago truncatula Genotypes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

molecular Plant 2012年第5期5卷 1068-1081页

作者： Ons Zahaf Sandrine Blanchet Axel de Zelicourt Benoit Alunni Julie Plet Carole Laffont Laura de Lorenzo Sandrine Imbeaud Jean-Laurent lchante Anouck Diet Mounawer Badri Ana Zabalza Esther M. Gonzalez Herve delacroix Veronique Gruber Florian Frugier Martin Crespi Institut des Sciences du Vegetal (ISV) CNRS 91198 Gif-sur-Yvette France Universite Paris Diderot Paris 7 75205 Paris Cedex 13 France departamento de Microbiologia y Parasitologia Universidad de Sevilla 41012 Sevilla Spain present address: Centro Nacional de Biotecnologia (CNB) CSIC 28049 Madrid Spain Centre de Genetique Moleculaire (CGM) CNRS Gif/Orsay DNA Microarray Platform (GODMAP) Universite Paris-Sud 11 91190 Gif-sur-Yvette France present address: Genomique Fonctionnelle des tumeurs solides INSERM IUH Universit~ Paris Descartes 75010 Paris France Laboratory of Legumes Centre of Biotechnology of Borj Cedria BP 901 2050 Hammam-Lif Tunisia departamento de Ciencias del Medio Natural Universidad P~blica de Navarra 31006 Pamplona Spain

Evolutionary diversity can be driven by the interaction of plants with different environments. molecular bases involved in ecological adaptations to abiotic constraints can be explored using genomic tools. Legumes are major crops worldwide and soil salinity is a main stress affecting yield in these plants. We analyzed in the Medicago truncatula legume the root transcriptome of two genotypes having contrasting responses to salt stress： TN1.11, sampled in a salty Tunisian soil, and the reference Jemalong A17 genotype. TN1.11 plants show increased root growth under salt stress as well as a differential accumulation of sodium ions when compared to A17. Transcriptomic analysis revealed specific gene clusters preferentially regulated by salt in root apices of TN1.11, notably those related to the auxin pathway and to changes in histone variant isoforms. Many genes encoding transcription factors （TFs） were also differentially regulated between the two genotypes in response to salt. Among those selected for functional studies, overexpression in roots of the A17 ge- notype of the bHLH-type TF most differentially regulated between genotypes improved significantly root growth under salt stress. despite the global complexity of the differential transcriptional responses, we propose that an increase in this bHLH TF expression may be linked to the adaptation of M. truncatula to saline soil environments.

关键词： root architecture abiotic stress legume transcriptome bHLH.

Analyses of Sexual Reproductive Success in Transgenic and/or Mutant Plants

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2009年第8期51卷 719-726页

作者： Cristiane P. G. Calixto Gustavo H. Goldman Maria Helena S. Goldman departamento de biologia Faculdade de Filosofia Ciências e Letras de Ribeirao PretoUniversidade de So Paulo 14040-901 Brazil PPG departamento de Gentica Faculdade de Medicina de Ribeiro PretoUniversidade de So Paulo 14049-900 Brazil departamento de C. Farmacêuticas Faculdade de Ciências Farmacêuticas de Ribeiro PretoUniversidade de So Paulo 14040-903 Brazil departamento de biologia Faculdade de Filosofia Ciências e Letras de Ribeirao Preto Universidade de So Paulo 14040-901 Brazil

The pistil, the female reproductive organ of plants, is a key player in the success of sexual plant reproduction. Ultimately, the production of fruits and seeds depends on the proper pistil development and function. Therefore, the identification and characterization of pistil expressed genes is essential for a better understanding and manipulation of the plant reproduction process. For studying the function of pistil expressed genes, transgenic and/or mutant plants for the genes of interest are used. The present article provides a review of methods already exploited to analyze sexual reproductive success. We intend to supply useful information and to guide future experiments in the study of genes affecting pistil development and function.

关键词： knockout mutant plants overexpression transgenic plants pistil expressed genes plant sexual reproduction reproductive success silenced transgenic plants.

The Arabidopsis COP9 SIGNALOSOME INTERACTING F-BOX KELCH 1 Protein Forms an SCF Ubiquitin Ligase and Regulates Hypocotyl Elongation

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

molecular Plant 2013年第5期6卷 1616-1629页

作者： Anna Franciosini Benedetta Lombardi Silvia lafrate Valeria.Pecce Giovanni Mele Leonardo Lupacchini Gianmarco Rinaldi youichi Kondou Giuliana Gusmaroli Shiori Aki Tomohiko Tsuge Xing-Wang deng Minami Matsui Paola Vittorioso Paolo Costantin Giovanna Serino Dipartimento di biologia e Biotecnologie Sapienza Universita di Roma Italy Istituto di biologia e Biotecnologia Agraria Consiglio Nazionale delle Ricerche Italy RIKEN Plant Science Center Yokohama Japan department of molecular Cellular and Development Biology Yale University New Haven CT USA present address: department of Biology University of South Carolina Beaufort Bluff'ton AC USA molecular Biology Laboratory Institute for Chemical Research Kyoto University Japan The New york Botanical Garden Bronx NY USA

The regulation of protein turnover by the ubiquitin proteasome system （UPS） is a major posttranslational mechanism in eukaryotes. One of the key components of the UPS, the COP9 signalosome （CSN）, regulates ＇cullin-ring＇ E3 ubiquitin ligases. In plants, CSN participates in diverse cellular and developmental processes, ranging from light signaling to cell cycle control. In this work, we isolated a new plant-specific CSN-interacting F-box protein, which we denominated CFK1 （COP9 INTERACTING F-BOX KELCH 1）. We show that, in Arabidopsis thaliana, CFK1 is a component of a functional ubiquitin ligase complex. We also show that CFK1 stability is regulated by CSN and by proteasome-dependent proteoly- sis, and that light induces accumulation of the CFK1 transcript in the hypocotyl. Analysis of CFK1 knockdown, mutant, and overexpressing seedlings indicates that CFK1 promotes hypocotyl elongation by increasing cell size. Reduction of CSN levels enhances the short hypocotyl phenotype of CFKl-depleted seedlings, while complete loss of CSN activity sup- presses the Iong-hypocotyl phenotype of CFKl-overexpressing seedlings. We propose that CFK1 （and its regulation by CSN） is a novel component of the cellular mechanisms controlling hypocotyl elongation.

关键词： Arabidopsis thaliana ubiquitin COP9 signalosome proteasome F-box protein hypocotyl elongation seed-ling development.

Vegetation structure and edaphic factors in veredas reflect different conservation status in these threatened areas

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Forest Ecosystems 2022年第3期9卷 387-395页

作者： yule Roberta Ferreira Nunes Camila Silveira Souza Islaine Francielly Pinheiro de Azevedo Odirlei Simôes de Oliveira Leidivan Almeida Frazão Rúbia Santos Fonseca Rubens Manoel dos Santos Walter Viana Neves Programa de Pós-Graduaçao Em Botânica Aplicada Departamento de Biologia GeralUniversidade Estadual de Montes ClarosAvenida Dr.Ruy BragaS/N-Bairro Vila MauricéiaMontes ClarosMinas Gerais39401-089Brazil Laboratório de Ecologia Vegetal Universidade Estadual de Montes ClarosAvenida Dr.Ruy BragaS/N-Bairro Vila MauricéiaMontes ClarosMinas Gerais39401-089Brazil Instituto de Ciências Agrarias-Universidade Federal de Minas Gerais Avenida Universitária1.000-Bairro Universit�arioMontes ClarosMinas Gerais39404-547Brazil departamento de Ciências Florestais Universidade Federal de LavrasCP 3037LavrasMinas Gerais37200-000Brazil Instituto Mineiro de Gestao dasÁguas Secretaria de Estado de Meio Ambiente e Desenvolvimento SustentávelAvenida JoséCorreia Machado900-Bairro IbiturunaMontes ClarosMinas Gerais39401-832Brazil

Background:In Cerrado humid areas,veredas are considered as hygrophilous communities,marked by the presence of the Mauritia flexuosa palm tree emerging in the wetter *** in veredas ecosystems due to drought and changes in the water table,which represent important areas for biodiversity maintenance in the Brazilian cerrado,can lead to the emergence of new landscapes and *** that the veredas are changed in species composition due to anthropogenic factors,in this study,we evaluated the flora composition and described the current vegetation profile of two veredas under different disturbances levels that are located in the north of Minas Gerais State,***:Altogether,2,268 individuals of 91 species belonging to 36 families were *** results demonstrated that the studied veredas were floristically different in terms of the arboreous-shrubby species composition,richness,and *** the hygrophilous forest formations and open areas in the two veredas present typical species from these environments(same species with high importance values),the drought in the water table caused an expansion of Cerrado species in the open areas and in the hygrophilous ***,in the hygrophilous forest there was an increase in the abundance of species that occur in less saturated ***:Our results demonstrate that the studied veredas are changing in species composition due to anthropogenic factors which leads to the drought of these formations(water balance effect).These anthropogenic effects cause a modification with the expansion and increase in the abundance of typical species of other areas of Cerrado and resistant to less saturated soils,leading to the veredas ***,future studies should investigate other veredas on a broader scale to detect changes in the water table that lead to a consequent change in vegetation *** this way,we will have support for the conscientious management and conservatio

关键词： Cerrado Conservation Palm swamp Plant distribution Climate change Water balance effect Vereda drought Phytosociological analysis

Resistance and susceptibility of alfalfa （Medicago sativa L.） cultivars to the aphid Therioaphis maculata （Homoptera： Aphididae）： insect biology and cultivar evaluation

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Insect Science 2006年第1期13卷 55-60页

作者： ALEXANDRE de ALMEIDA E SILVA ELENICE MOURO VARANDA JOSE RICARDO BAROSELA Laboratorio de Entomologia IPEPATRO RO Brasil departamento de biologia Faculdade de Filosofia Ciencias e Letras de Ribeirao Preto Universidade de Sao Paulo Ribeirao Preto SP Brazil

Biology of the aphid Therioaphis maculata was studied on alfalfa （Medicago sativa L.）, including four resistant （Mesa-Sirsa, CUF101, Baker and Lahontan） and two susceptible （ARC and Caliverde） alfalfa cultivars, and one of the most cropped Brazilian cultivars, Crioula. Under controlled conditions, antibiosis （i.e., reduced longevity, fecundity and increased mortality of the aphid） was observed mainly on the resistant alfalfa cultivars, except on Lahontan. Crioula seemed to be tolerant to aphids. present data support geographic limitation usage of cultivars, and we suggest Baker and Mesa-Sirsa as sources of antibiosis, and provide biological information of a tropical T. maculata biotype on alfalfa.

关键词： alfalfa cultivars aphid resistance susceptibility Therioaphis maculata

Arabidopsis Heterotrimeric G-protein Regulates Cell Wall defense and Resistance to Necrotrophic Fungi

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

molecular Plant 2012年第1期5卷 98-114页

作者： Magdalena delgado-Cerezo Clara Sanchez-Rodriguez Viviana Escudero Eva Miedes Paula Virginia Fernandez Lucia Jorda Camilo Hernandez-Blanco Andrea Sanchez-Vallet Pawel Bednarek Paul Schulze-Lefert Shauna Somervilleg Jose Manuel Estevez Staffan Persson Antonio Molina Centro de Biotecnologia y Genomica de Plantas （UPM-INIA） Universidad Politecnica de Madrid Campus de Montegancedo E-28223-Pozuelo de Alarcbn （Madrid） Spain Max-Planck-lnstitute fLir Molekulare Pflanzenphysiologie Am MQhlenberg 2 14476 Potsdam Germany Laboratorio de Fisiologia y biologia molecular IFIByNE （CONICET） FCEyN-Universidad de Buenos Aires Pab. II Intendente Guiraldes 2160 Buenos Aires C1428EGA Argentina present address： Laboratory of Phytopathology Wageningen University Droevendaalsesteeg 1 6708 PB Wageningen The Netherlands Max Planck Institut for Zuchtungsforschung Department of Plant Microbe Interactions Carl-von-Linne-Weg 10 D-50829 Koln Germany Institute of Bioorganic Chemistry Polish Academy of Sciences Noskowskiego 12/14 61-704 Poznan Poland Energy Biosciences Institute 130 Calvin Hall University of California at Berkeley Berkeley CA 94720 USA

The Arabidopsis heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi. The agbl mutant impaired in the Gβ subunit displays enhanced susceptibility to these pathogens. Gβ/AGB1 forms an obligate dimer with either one of the Arabidopsis Gγsubunits （γ1/AGG1 and γ2/AGG2）. Accordingly, we now demonstrate that the aggl agg2 double mutant is as susceptible as agbl plants to the necrotrophic fungus Plectosphaerella cucumerina. To elucidate the molecular basis of heterotrimeric G-protein-mediated resistance, we performed a comparative transcriptomic analysis of agbl-1 mutant and wild-type plants upon inoculation with P cucumerina. This analysis, together with metab- olomic studies, demonstrated that G-protein-mediated resistance was independent of defensive pathways required for resistance to necrotrophic fungi, such as the salicylic acid, jasmonic acid, ethylene, abscisic acid, and tryptophan-derived metabolites signaling, as these pathways were not impaired in agbl and aggl agg2 mutants. Notably, many mis-reguiated genes in agbl plants were related with cell wall functions, which was also the case in aggl agg2 mutant. Biochemical analyses and Fourier Transform InfraRed （FTIR） spectroscopy of cell walls from G-protein mutants revealed that the xylose content was lower in agbl and aggl agg2 mutants than in wild-type plants, and that mutant walls had similar FTIR spec-tratypes, which differed from that of wild-type plants. The data presented here suggest a canonical functionality of the Gβ and Gγ1/γ2 subunits in the control of Arabidopsis immune responses and the regulation of cell wall composition.

关键词： Plant immunity cell wall indole glucosinolates xylose necrotropic fungi G-protein.

Maximum Entropy Ecological Niche Prediction of the Current Potential Geographical Distribution of <i>Eimeria</i>Species of Cattle, Sheep and Goats in Mexico

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Animal Sciences 2019年第2期9卷 234-248页

作者： yazmin Alcala-Canto Aldo Alberti-Navarro Juan Antonio Figueroa-Castillo Froylan Ibarra-Velarde yolanda Vera-Montenegro Maria Eugenia Cervantes-Valencia Facultad de Medicina Veterinaria y Zootecnia Departamento de Parasitología UNAM Mexico City Mexico Facultad de Medicina Veterinaria y Zootecnia Departamento de Medicina y Zootecnia de Rumiantes UNAM Mexico City Mexico Facultad de Medicina Departamento de Biologia Celular y Tisular UNAM Mexico City Mexico

Coccidiosis is a gastrointestinal disease caused by parasites of the genus Eimeria. To produce the ecological niche model for the geographic distribution of Eimeria species, the maximum entropy algorithm (MaxEnt) was used and 19 bioclimatic variables with a spatial resolution of 30 arc-seconds (approximately 1 km2) were downloaded from the World Climate Database. These were reduced to BIO2, BIO3, BIO4, BIO7 and BIO15 for each species after examining cross-correlations among them to account multicollinearity. A jackknife analysis was included to assess the contribution of five bioclimatic variables and the fit of the model was evaluated with the area under receiver operating characteristic curve (AUC). Under a current climate scenario, the jackknife evaluation of the MaxEnt model showed that BIO4 (temperature seasonality) made the greatest contribution to the distribution model for 22 Eimeria species;whereas BIO7 (temperature annual range) was the most important factor that contributes to the distribution model of 10 species. The habitat suitability model based on the maximum entropy theory was supported by AUC values higher than 0.9 and predicted that the suitable habitats for different species of Eimeria are present in southern, eastern and western areas of Mexico. Our study may support future studies exploring factors that constrain the distribution of Eimeria as well as strategies aimed at reducing the disease prevalence.

关键词： Eimeria MaxEnt Ruminants Coccidiosis Habitat Suitability

Soil attributes structure plant assemblages across an Atlantic forest mosaic

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Plant Ecology 2018年第4期11卷 613-622页

作者： Maria Fabíola Barros Bruno X.Pinho Tarciso Leão Marcelo Tabarelli Programa de Pós-Graduação em biologia Vegetal Departamento de BotânicaUniversidade Federal de PernambucoPE 50670-901Brazil department of Forest Resources University of MinnesotaSt.PaulMN 55108USA

Aims Community assembly persists as a key topic in ecology due to the complex variation in the relative importance of assembly forces and mechanisms across spatio-temporal scales and *** we address a forest-savanna vegetation mosaic in the Brazilian Atlantic forest to examine the role played by soil attributes as determinants of community assembly and organiza-tion at a landscape spatial *** We examined soil and plant assemblage attributes across 23 plots of forest and savanna in a 1600 km2 landscape exposed to the same climatic conditions in the Atlantic forest region of northeast *** attributes included species richness,taxonomic and functional composition(community weighted mean,CWM)and functional diversity(quadratic diversity;Rao’s quadratic entropy index)relative to plant leaf area,specific leaf area,leaf dry matter content,thickness and *** Findings Our results suggest that forest and savanna patches exposed to the same climatic conditions clearly differ in terms of soil attributes,plant assemblage structure,taxonomic and functional *** selecting particular plant strategies relative to resource economy,soil potentially affects community structure,with forest assemblages bear-ing more acquisitive resource-use strategies,while conservative plant strategies are more frequent in ***,savanna-forest mosaics in the Atlantic forest region represent spatially organized plant assemblages in terms of taxonomic and functional features,with a sig-nal of trait convergence in both vegetation ***-mediated filter-ing thus emerges as a potential deterministic assembly force affecting the spatial organization of savanna-forest boundaries and mosaics.

关键词： community assembly habitat filtering plant functional traits savanna vegetation tropical forest