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Screening ulcerative colitis key target gene and pathway based on KEGG pathway

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precision medicine Research 2020年第1期2卷 17-25页

作者： Zhi-Hua Yang Yan Liu Yi-Hua Fan Hai-Feng Yan First Teaching Hospital of Tianjin University of Traditional Chinese medicine Tianjin 300381China Tianjin Nankai Hospital Tianjin 300100China

Objective:To obtain the relevant information of pathway and gene by using the database of DAVID,analyze the function and distribution of important genes,screen out the target genes related to Ulcerative Colitis and promote the study of the pathogenesis of UC and the development of new ***:The Ulcerative Colitis was used to search UC related genes in TTD,Drugbank,DisGeNET *** obtained gene data was input to the database of Daved,and the data of gene enriched pathway was obtained 87 genes were Significant enriched in the first 20 KEGG *** 87 genes were input to the string database to make the interaction network diagram,and the key genes enriched in the pathway were also made the network diagram,and the two network diagrams were ***:RELA,TNF,IL1B,NFKB1,IL6 and IL10 were among the highest ranked genes in two network ***:The study of RELA,TNF,IL1B,NFKB1,IL6 and IL10 is necessary for us to study *** pathogenesis of UC was associated with multiple pathways such as NF-kappa B signaling pathway,Jak-STAT signaling pathway,TNF signaling pathway and so *** is helpful to understand the pathogenesis of disease and provide a reliable target for the development of new drugs by analyzing the pathway and the network diagram of the interaction between genes and disease related genes.

关键词： Ulcerative Colitis KEGG pathway Target gene,Pathway

T1 and ECV Mapping in Myocardial Disease

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Cardiovascular Innovations and Applications 2016年第B12期 73-84页

作者： Eric L.Olausson Erik B.Schelbert Department of Clinical Physiology Karolinska Institutet and Karolinska University HospitalStockholmSweden Department of medicine University of Pittsburgh School of MedicinePittsburghPAUSA UPMC Cardiovascular Magnetic Resonance Center Heart and Vascular InstitutePittsburghPAUSA Clinical and Translational Science Institute University of PittsburghPittsburghPAUSA

T1 mapping using cardiovascular magnetic resonance(CMR)introduces novel techniques for myocardial tissue characterization to detect and quantify disease processes occurring at the microscopic *** though T1 mapping has limited spatial resolution,cellular and molecular changes occurring within each voxel can affect the aggregate T1 signal rendering them quantifi *** estimated T1-based parameters quantifi ed on a“map”demonstrate the spatial localization of these changes whereby each pixel expresses the quantitative value of that *** quantifi cation permits detection of diffuse disease even if it is not directly *** than relying on nonspecifi c functional measures,T1 mapping focuses on intrinsic changes of myocardial composition that advances understanding about specifi c disease *** changes in myocardial tissue composition inform diagnosis and prognosis.T1 mapping encompasses two key parameters:native(i.e.,precontrast)T1 and extracellular volume fraction(ECV)derived from additional postcontrast T1 and blood T1 *** advances introduce new tools to detect focal and diffuse myocardial derangements occurring in cardiac disease that can be otherwise diffi cult to detect.T1 and ECV mapping foster precision medicine and personalized care,promising to improve patient outcomes through targeted *** on the opportunities introduced by T1 mapping and ECV requires further investigation.

关键词： T1 mapping extracellular volume myocardial fi brosis remodeling amyloidosis

The predictive value of preoperative albumin-to-globulin ratio in patients with hilar cholangiocarcinoma

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precision medicine Research 2021年第2期3卷 1-7页

作者： Hong-Gen Liu Liu Yong Ying-Jie Jia Department of Oncology First Teaching Hospital of Tianjin University of Traditional Chinese MedicineTianjimChina Department of Gastrointestinal Oncology Department of Oncology First Teaching Hospital of Tianjin University of Iraditional Chinese medicine Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for Cancer300060TianjinChina

Background:To investigate the associations between preoperative albumin-to-globulin ratio and clinicalpathological factors,and the impacts of albumin-to-globulin ratio on the longterm outcomes and postoperative complications in hilar ***:Ninety-seven hilar cholangiocarcinoma patients were enrolled in our *** clinicalpathological and survival information were *** analysis was using chi-square test and logistic *** analysis was done by Log-rank test,multivariate analysis was performed using the Cox proportional hazard regression ***:The optimal cutoff value of the preoperative albumin-to-globulin ratio was set at 1.34,The albumin-to-globulin ratio level was elevated in 49 hilar cholangiocarcinoma patients,while other 48 hilar cholangiocarcinoma patients were with low *** stage(OR=2,621;P=0.003)and lymph node metastasis(OR=3.704;P=0.004)were identified as independently risks of albumin-to-globulin ratio level in hilar cholangiocarcinoma *** Cox proportional hazard regression model found differentiation(HR=1.989;P=0.014),tumor stage(HR=1.480;P=0.014)and albumin-to-globulin ratio level(HR=0.037;P=0.017)were identified as the independent factors of the overall ***,we found the low albumin-toglobulin ratio level(OR=0.037;P=0.017)was an independent risk factor for the incidence of postoperative ***:Preoperative albumin-to-globulin ratio is a simple and useful marker not only to identify patients at increased risk for postoperative complications,but also to predict long-term survival.

关键词： Hilar cholangiocarcinoma Albumintoglobulin ratio Prognosis Postoperative complications

Screening key target genes for coronavirus disease 2019 based on bioinformatics and gene network

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precision medicine Research 2020年第3期2卷 95-103页

作者： Zhi-Hua Yang Yuan-Yuan Liu Hai-Feng Yan Zhao-Ge Hao-Jia Chen Lin-Wang Ting-Ting Lv First Teaching Hospital of Tianjin University of Traditional Chinese medicine Tianjin 300381China Jiangsu College of Nursing Huaian 475300China

Objective:To use bioinformatics and gene networks to screen key target genes of coronavirus disease 2019,which provides references for clinical research and development of drugs for coronavirus disease ***:Target genes related to coronavirus disease 2019 were screened in the GeneCards and National Center for Biotechnology Information databases,and the obtained gene data were imported into the Database for Annotation,Visualization and Integrated Discovery(Version 6.8)database to collect the related information about pathways and *** genes enriched in the first 20 pathways and the genes whose occurrence frequency≥5 were imported into the String database respectively to construct protein-protein interaction network diagram and compare the two network ***:TNF,IL-6,IL-2,IL-8,CXCL8,IL1B,CCL2,IFNG,STAT1,MAPK1,MAPK3,MAPK8,TP53 and RELA are ranked top in the two network diagrams,and the frequency of occurrence in the first 20 pathways was≥***:The incidence of coronavirus disease 2019 is associated with multiple signaling pathways,including influenza A,pathways in cancer,toll-like receptor signaling pathway,hypoxia-inducible factor-1 signaling pathway,et ***,IL-6,IL-2,IL-8,CXCL8,IL1B,CCL2,IFNG,STAT1,MAPK1,MAPK3,MAPK8,TP53 and RELA are closely related to coronavirus disease 2019,which needs to be further *** analyzing the pathways of the genes related to coronavirus disease 2019 and the interactive network diagrams between the genes,it is helpful to understand the pathogenesis of the disease and provide a reference for clinical research and development of effective drugs for coronavirus disease 2019.

关键词： Coronavirus disease 2019 Severe acute respiratory syndrome coronavirus 2 Bioinformatics Gene network Tumor necrosis factor Interleukin-6

Pig-to-Human xenotransplantation:Moving toward organ customization

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precision Clinical medicine 2023年第2期6卷 119-120页

作者： Mengyu Gao Jie Zhang Rui Wang Guang Yang Ji Bao Transplant Center Key Laboratory of Transplant Engineering and ImmunologyInstitute of Clinical PathologyWest China HospitalSichuan UniversityChengdu 610041China SCMPA Key Laboratory for Quality Research and Control of Chemical medicine Chengdu Institute for Drug ControlChengdu 610041China Experimental Animal Center West China HospitalSichuan UniversityChengdu 610041China

Dear Editor,Xenotransplantation is a potentially effective way to address the shortage of organ *** improvements in gene editing technology have accelerated a number of a breakthrough in genetically modifying pigs for... 详细信息

关键词： breakthrough kidney Human

Improvement of TLC Identification of Matrine in Jin Weng Zhi Li Granule

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Medicinal Plant 2017年第5期8卷 58-61页

作者： Yongfeng HAO Qingchang YANG Li LIU Dengfang CHENG Xianchao YANG Juan LIU Xuehong HU Traditional Chinese medicine Innovation Laboratory Rongchang Campus of Southwest University Veterinary Research Institute of Sichuan Baer Agriculture and Animal Husbandry Company

[Objectives] The aim of this study was to screen out the TLC conditions of matrine in Jin Weng Zhi Li Granule. [Methods]Referring to the method of Chinese Veterinary Pharmacopoeia,the composition,chromogenic agent and expansion conditions of the developer were optimized,and matrine was qualitatively identified by TLC. [Results] After optimizing TLC conditions of Jin Weng Zhi Li Granule,that is,the thin layer plate was pre-equilibrated in the expansion cylinder for 5 min after spotting,the separation effect of TLC of test samples was favorable. The spot color was clear,reproducibility was good,precision was high,specificity was better. [Conclusions] The method was of good feasibility,reproducibility and specificity,and could be used for qualitative detection of matrine in Jin Weng Zhi Li Granule dampnessheat and diarrhea disease.

关键词： TLC Identification Matrine Jin Weng Zhi Li Granule

Screening of TLC Identification of Forsythin in Jin Weng Zhi Li Granule

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Medicinal Plant 2017年第5期8卷 62-64页

作者： Li LIU Dengfang CHENG Xianchao YANG Qingchang YANG Juan LIU Si QIN Yunli ZHANG Xuehong HU Traditional Chinese medicine Innovation Laboratory Rongchang Compus of Southwest University Veterinary Research Institute of Sichuan Baer Agriculture and Animal Husbandry Company

[Objectives] To screen TLC conditions of forsythin contained in Jin Weng Zhi Li Granule. [Methods] According to the method described in Chinese Veterinary Pharmacopoeia,thin layer chromatography was used for qualitative identification of forsythin contained in Jin Weng Zhi Li Granule by improving the developing conditions. [Results]After optimizing TLC conditions of Jin Weng Zhi Li Granule,that is,the thin layer plate was pre-equilibrated in the expansion cylinder for 5 min after spotting,the separation effect of TLC of test samples was favorable. The spot color was clear,reproducibility was good,precision was high,specificity was better. [Conclusions]The method is of good feasibility,reproducibility and specificity,and could be used for qualitative detection of forsythin contained in Jin Weng Zhi Li Granule.

关键词： TLC Forsythin Jin Weng Zhi Li Granule

Detection of Berberine Hydrochloride in Jin Weng Zhili Particle Using TLC and HPLC

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Medicinal Plant 2017年第1期8卷 12-16页

作者： Li LIU Lifang OUYANG Dengfang CHENG Juan LIU Xuehong HU Yuan LI Traditional Chinese medicine Innovation Laboratory Rongchang Compus of Southwest University Veterinary Research Institute of Sichuan Baer Agriculture and Animal Husbandry Company

[Objectives]To screen optimum conditions of HPLC and TLC for qualitative discrimination of berberine hydrochloride contained in Jin Weng Zhili Particle,thus providing basis for quality standard. [Methods]According to the method described in Chinese Veterinary Pharmacopoeia,thin layer chromatography and high performance liquid chromatography were used respectively for qualitative and quantitative identification of berberine hydrochloride contained in Jin Weng Zhili Particle by improving the conditions of extraction and purification. [Results]After optimizing TLC conditions of Jin Weng Zhili Particle,the separation effect of TLC of test samples was favorable. The spot color was clear,reproducibility was good,and precision was high. Under the conditions of mobile phase constituted by acetonitrile-water-glacial acetic acid-triethylamine( 28∶ 70∶ 1∶ 1),detection wavelength of 265 nm,flow rate of 1. 0 m L min^(-1)and column temperature of 35℃,the resolution of berberine hydrochloride was good; the injection volume in the range of 3. 15-50 μg· m L^(-1)had good linear relationship with the peak area( R^2= 0. 998); the average sample recovery rate(n=6) was 5. 672%,and RSD was 0. 864%; the content of berberine hydrochloride contained in Jin Weng Zhili Particle was 4. 023 mg. [Conclusions]The method was of good feasibility,reproducibility and specificity,and could be used for qualitative and quantitative detection of berberine hydrochloride contained in Jin Weng Zhili Particle.

关键词： HPLC TLC Berberine hydrochloride Jin Weng Zhili Particle

The SARS-CoV-2 spike L452R-E484Q variant in the Indian B.1.617 strain showed significant reduction in the neutralization activity of immune sera

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precision Clinical medicine 2021年第3期4卷 149-154页

作者： Gen Li Zhongcheng Zhou Peng Du Meixing Yu Ning Li Xinxin Xiong Hong Huang Zhihai Liu Qinjin Dai Jie Zhu Chengbin Guo ShanyunWu Daniel T.Baptista-Hon Man Miao LamWai Ming Yong Wu Fanxin Zeng Charlotte L.Zhang Edward D.Zhang Haifeng Song Jianghai Liu Johnson Yiu-Nam Lau Andy P.Xiang Kang Zhang Guangzhou Women and Children’s Medical Center Guangzhou Medical UniversityGuangzhou 510620China University Hospital and Center for Biomedicine and Innovations Faculty of MedicineMacao University of Science and TechnologyMacao 030027China Jinan University First Affiliated Hospital Jinan UniversityGuangzhou 510630China Department of Clinical Research Center Dazhou Central HospitalDazhou 635099China Department of Bioinformatics and AI Bioland LaboratoryGuangzhou 510000China ABLINK Biotech Co. Chengdu 610000China Department of Applied Biology and Chemical Technology Hong Kong Polytechnic UniversityHung HomHong Kong 610051China Center for Stem Cell Biology and Tissue Engineering Key Laboratory for Stem Cells and Tissue EngineeringMinistry of EducationSun Yat-sen UniversityGuangzhou 510000China

To assess the impact of the key non-synonymous amino acid substitutions in the RBD of the spike protein of SARS-CoV-2 variant B.1.617.1(dominant variant identified in the current India outbreak)on the infectivity and neutralization activities of the immune sera,L452R and E484Q(L452R-E484Q variant),pseudotyped virus was constructed(with the D614G background).The impact on binding with the neutralizing antibodies was also assessed with an ELISA *** virus carrying a L452R-E484Q variant showed a comparable infectivity compared with ***,there was a significant reduction in the neutralization activity of the immune sera from non-human primates vaccinated with a recombinant receptor binding domain(RBD)protein,convalescent patients,and healthy vaccinees vaccinated with anmRNA *** addition,there was a reduction in binding of L452R-E484Q-D614G protein to the antibodies of theimmune sera fromvaccinated nonhuman *** results highlight the interplay between infectivity and other biologic factors involved in the natural evolution of *** neutralization activities against the L452R-E484Q variant will have an impact on health authority planning and implications for the vaccination strategy/newvaccine development.

关键词： SARS-CoV-2 COVID-19 B.1.617.1 L452R E484Q infectivity immune neutralization