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Handmade cloning:recent advances,potential and pitfalls

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Journal of Animal Science and Biotechnology 2015年第4期6卷 378-387页

作者： Geetika Verma JS Arora RS Sethi CS Mukhopadhyay Ramneek Verma School of Animal Biotechnology Post Graduate Institute of Veterinary Education and ResearchGuru Angad Dev Veterinary and Animal Sciences University

Handmade cloning （HMC） is the most awaited, simple and micromanipulator-ffee version of somatic cell nuclear transfer （SCNT）. The requirement of expensive micromanipulators and skilled expertise is eliminated in this technique, proving it as a major revolution in the field of embryology. During the past years, many modifications have been incorporated in this technique to boost its efficiency. This alternative approach to micromanipulator based traditional cloning O-C） works wonder in generating comparable or even higher birth rates in addition to declining costs drastically and enabling cryopreservation. This technique is not only applicable to intraspecies nuclear transfer but also to interspecies nuclear transfer （iSCNT） thus permitting conservation of endangered species. It also offers unique possibilities for automation of SCNT which aims at production of transgenic animals that can cure certain human diseases by producing therapeutics hence, providing a healthier future for the wellbeing of humans. The present review aims at highlighting certain aspects of HMC including recent advancements in procedure and factors involved in elevating its efficiency besides covering the potentials and pitfalls of this technique

关键词： Handmade cloning Micromanipulator Somatic cell nuclear transfer Transgenic Zona free cloning

Olanzapine-induced leucopaenia and thrombocytopaenia in an elderly patient:a case report and review of the evidence

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General Psychiatry 2018年第5期31卷 111-114页

作者： Yogender Kumar Malik Swapnajeet Sahoo Ajit Avasthi Department of Psychiatry Post Graduate Institute of Medical Education and ResearchChandigarhIndia

Haematological adverse effects of antipsychotics are rare but life threatening. Existing literature is limited to case reports, which are mostly reported on second generation antipsychotics(clozapine, olanzapine, risperidone, quetiapine). Elderly individuals are at risk of developing side effects with any psychotropics. Olanzapine is commonly used for the management of psychotic symptoms as well as for the management of behavioural and psychological problems with dementia in the elderly. In this case report, the authors report thrombocytopaenia and leucopaenia in an elderly individual with schizophrenia which developed after initiation of olanzapine and reverted back after stoppage of the drug. This case report highlights that the elderly are susceptible to develop haematological side effects with olanzapine and hence monitoring may be essential.

关键词： generation antipsychotics management of behavioural

Effect of disturbance on biomass,production and carbon dynamics in moist tropical forest of eastern Nepal

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Forest Ecosystems 2016年第3期3卷 221-230页

作者： Tilak Prasad Gautam Tej Narayan Mandal Department of Botany Mahendra Morang Adarsha Multiple CampusTribhuvan University Department of Botany Post Graduate Campus Tribhuvan University

Background： Forest biomass is helpful to assess its productivity and carbon （C） sequestration capacity. Several disturbance activities in tropical forests have reduced the biomass and net primary production （NPP） leading to climate change. Therefore, an accurateestimation of forest biomass and C cycling in context of disturbances is required for implementing REDD （Reducing Emissions from Deforestation and Forest Degradation） policy. Methods： Biomass and NPP of trees and shrubs were estimated by using allometric equations while herbaceous biomass was estimated by harvest method. Fine root biomass was determined from soil monolith. The C stock in vegetation was calculated by multiplying C concentration to dry weight. Results： Total stand biomass （***-1） in undisturbed forest stand （US） was 960.4 while in disturbed forest stand （DS） it was 449.1. The biomass （***-1） of trees, shrubs and herbs in US was 948.0, 4.4 and 1.4, respectively, while in DS they were 438.4, 6.1 and 1.2, respectively. Total NPP （***-1） was 26.58 （equivalent to 12.26 Mg ***-1） in US and 14.91 （6.88 Mg ***-1） in DS. Total C input into soil through litter plus root turnover was 6.78 and 3.35 ***-1 in US and DS, respectively. Conclusions： Several disturbance activities resulted in the significant loss in stand biomass （53 %）, NPP （44 %）, and C sequestration capacity of tropical forest vegetation is far greater than that returned to the soil n eastern Nepal. The net uptake of carbon by the by the turnover of fine root and litter. Therefore, both stands of present forest act as carbon accumulating systems. Moreover, disturbance reflects higher C emissions which can be reduced by better management.

关键词： Tropical forest Disturbance Biomass Production Carbon cycling Nepa

Primary tuberculosis of the thyroid gland:a case report

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Asian Pacific Journal of Tropical Biomedicine 2012年第10期2卷 839-840页

作者： Sant Parkash Kataria Parul Tanwar Sneh Singh Sanjay Kumar Department of Pathology Post Graduate Institute of Medical SciencesRohtak

Tuberculosis of the thyroid gland is an uncommon disease and primary involvement of Uiyroid is even more *** is a rare disease even in countries in which tuberculosis is *** diagnosis is often difficult as the clinical presentation has no distinct *** course of the disease may resemble toxic goiter or acute thyroiditis or may follow a subacute or chronic growth pattern without specific *** presence of necrotizing epithelioid cell granulomas along with langhans type giant cells are the hallmark of thyroid *** of acid fast bacilli by ZN staining confirms the diagnosis,but this stain is frequently negative in tissue sections.

关键词： Tuberculosis Thyroid Granuloma

硫酸存在时N-溴代苯二甲酰亚胺对甘露糖的胶束催化氧化(英文)

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物理化学学报 2009年第2期25卷 319-326页

作者： KATRE Yokraj SINGH Minu PATIL Sangeeta SINGH Ajaya K. Department of Chemistry Kalyan Post Graduate College Bhilai Nagar Department of Chemistry Vishwanath Yadav Tamaskar Post Graduate Autonomous College

The kinetics of micellar-catalyzed oxidation of mannose by N-bromophthalimide was studied in the presence of sulfuric acid at 313 K. The orders of reaction with respect to [mannose], [oxidant], and [H+] were found to be fractional, first, and negative fractional order, respectively. Anionic micelles of sodium dodecyl sulfate showed a partial inhibitory effect, while cationic micelles of cetyltrimethylammonium bromide increased the reaction rate with the same kinetic behavior. The reaction was catalyzed by cationic micelles, because of favorable electrostatic/thermodynamic/ hydrophobic/hydrogen bonding between reactants and cationic micelles. Their catalytic roles are best explained by Berezin′s model. A variation of [phthalimide] showed that the rate of reaction decreased with increasing [phthalimide]. It was observed that, an increase of [mercuric acetate] had no effect on reaction velocity. The influence of salts on the reaction rate was also studied. The rate constant (kW), binding constants (KS+KO), and corresponding activation parameters (Ea, △H#, △S#, and △G#) were determined. A detailed mechanism with associated reaction kinetics is presented and discussed.

关键词：胶枣催化氧化动力学甘露糖

Antispermatogenic and hormonal effects of Crotalaria junceaLinn. seed extracts in male mice

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Asian Journal of Andrology 2004年第1期6卷 67-70页

作者： B. Vijaykumar I. Sangamma A. Sharanabasappa Saraswati B. Patil Laboratory of Reproductive Biology Department of Post-Graduate Studies and Research in Zoology Gulbarga University Gulbarga-585 106 India

Aim: To evaluate the antifertility activity of various extracts of Crotalaria juncea seeds in male mice. Methods: Adult male mice were gavaged the petroleum ether, benzene and ethanol extracts of C. juncea seeds, 25 mg·(100g)-1·day-1 for 30 days. On day 31 the animals were sacrificed by cervical dislocation and the testes, epididymis, vas deferens, seminal vesicles, prostate gland, bulbourethral gland and levator ani were dissected out and weighed. The organs were processed for biochemical and histological examination. Results: In petroleum ether, benzene and ethanol extracts treated rats, there was a decrease in the weights of testis and accessory reproductive organs. The diameters of the testis and seminiferous tubules were decreased. Spermatogonia, spermatocytes and spermatids in the testis and the sperm count in cauda epididymis were also decreased. There was a significant reduction in the protein and glycogen contents and an increase in the cholesterol content in the testis, epididymis and vas deferens. Of the 3 extracts, the ethanol extract appeared to be the most potent in antispermatogenic activity. When the ethanol extract was tested in immature male mice, there was an antiandrogenic effect as the weights of accessory organs were reduced. Conclusion: The various extracts of C. juncea seeds arrest spermatogenesis and are likely to have an antiandrogenic activity.

关键词： Crotalaria juncea testis accessory reproductive organs antispermatogenic effect antiandrogenic effect

Portal Cavernoma Cholangiopathy in Children and the Management Dilemmas

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Journal of Clinical and Translational Hepatology 2020年第1期8卷 61-68页

作者： Moinak Sen Sarma Aathira Ravindranath Department of Pediatric Gastroenterology Sanjay Gandhi Post Graduate Institute of Medical SciencesLucknowUttar PradeshIndia

Portal cavernoma cholangiopathy(PCC)is one of the most harrowing complications of extrahepatic portal venous obstruc-tion,as it determines the long-term hepatobiliary *** symptomatic PCC is rare in children,asymptomatic PCC is as common as that in ***,there are major gaps in the literature with regard to the best imaging strategy and management modality in ***,natural history of PCC and effect of portosystemic shunt surgeries in children are *** PCC would lead to difficult or recalcitrant biliary strictures that will require endoscopic ther-apy or bilioenteric anastomosis,both of which are challenging in the presence of extensive *** are limited studies on the effect of portosystemic shunt surgeries on the outcome of PCC in children compared to *** this review,we aimed to collate all existing literature on PCC in childhood and also compare with adult *** highlight the difficul-ties of this disease to provide a comprehensive platform to foster further research on PCC exclusively in children.

关键词： Extra-hepatic portal vein obstruction Portal cavernoma cholangiopathy Portosystemic shunt

Treatment of spinal cord injury with biomaterials and stem cell therapy in non-human primates and humans

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Neural Regeneration Research 2025年第2期20卷 343-353页

作者： Ana Milena Silva Olaya Fernanda Martins Almeida Ana Maria Blanco Martinez Suelen Adriani Marques PhD Program in Pathological Anatomy Federal University of Rio de JaneiroRio de JaneiroBrazil Biomedical Science Institute Federal University of Rio de JaneiroRio de JaneiroBrazil graduate Program in Pathological Anatomy Federal University of Rio de JaneiroRio de JaneiroBrazil graduate Program in Pathological Anatomy(PPGAP/UFRJ) Department of Neurobiology/Institute of BiologyCampus do GragoatáNiteróiRio de JaneiroBrazil

Spinal cord injury results in the loss of sensory,motor,and autonomic functions,which almost always produces permanent physical ***,in the search for more effective treatments than those already applied for years,which are not entirely efficient,researches have been able to demonstrate the potential of biological strategies using biomaterials to tissue manufacturing through bioengineering and stem cell therapy as a neuroregenerative approach,seeking to promote neuronal recovery after spinal cord *** of these strategies has been developed and meticulously evaluated in several animal models with the aim of analyzing the potential of interventions for neuronal repair and,consequently,boosting functional *** the majority of experimental research has been conducted in rodents,there is increasing recognition of the importance,and need,of evaluating the safety and efficacy of these interventions in non-human primates before moving to clinical trials involving therapies potentially promising in *** article is a literature review from databases(PubMed,Science Direct,Elsevier,Scielo,Redalyc,Cochrane,and NCBI)from 10 years ago to date,using keywords(spinal cord injury,cell therapy,non-human primates,humans,and bioengineering in spinal cord injury).From 110 retrieved articles,after two selection rounds based on inclusion and exclusion criteria,21 articles were ***,this review arises from the need to recognize the experimental therapeutic advances applied in non-human primates and even humans,aimed at deepening these strategies and identifying the advantages and influence of the results on extrapolation for clinical applicability in humans.

关键词： bioengineering biomaterials cell therapy humans non-human primates spinal cord injury stem cell therapy

PERFORMANCE MODELING AND ANALYSIS OF BLOOD FLOW IN ELASTIC ARTERIES

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Applied Mathematics and Mechanics(English Edition) 2005年第3期26卷 345-354页

作者： Anil Kumar C.L.Varshney G.C.Sharma Department of post-graduate Studies and Research in Mathematics &Computer Science S.Varshney College Aligarh-202001 IndiaDepartment of Post-Graduate Studies and Research in Mathematics &Computer Science S.Varshney College Aligarh-202001 IndiaInstitute of Basic Science Khandari Agra-282002 India

Two different non_Newtonian models for blood flow are considered,first a simple power law model displaying shear thinning viscosity,and second a generalized Maxwell model displaying both shear thinning viscosity and oscillating flow viscous_*** models are used along with a Newtonian model to study sinusoidal flow of blood in rigid and elastic straight arteries in the presence of magnetic *** elasticity of blood does not appear to influence its flow behavior under physiological conditions in the large arteries,purely viscous shear thinning model should be quite realistic for simulating blood flow under these *** using the power law model with high shear rate for sinusoidal flow simulation in elastic arteries,the mean and amplitude of the flow rate were found to be lower for a power law fluid compared to Newtonian fluid for the same pressure *** governing equations have been solved by Crank_Niclson *** results are interpreted in the context of blood in the elastic arteries keeping the magnetic effects in *** physiological flow simulation in the aorta,an increase in mean wall shear stress,but a reduction in peak wall shear stress were observed for power law model compared to a Newtonian fluid model for matched flow rate wave *** flow in the presence of transverse magnetic field in an elastic artery is investigated and the influence of factors such as morphology and surface irregularity is evaluated.

关键词： elastic artery model Crank-Niclson scheme non-Newtonian fluid wall shear stress