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ISG15 and ISGylation:Emergence in the cytoskeleton dynamic and tumor microenvironment

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BIOCELL 2022年第5期46卷 1209-1213页

作者： ANGELES C.TECALCO-CRUZ posgrado en Ciencias Genómicas Universidad Autónoma de la Ciudad de México(UACM)Ciudad de México03300México

Cytoskeletal remodeling affects the shape,adhesion,and motility of cells.Cytoskeletal dynamics are modulated by matrix proteins,integrins,and several cytokines in the tumor microenvironment.In this scenario,signaling is activated by integrins and interferons,which can induce ISG15 gene expression.This gene encodes a ubiquitin-like protein that functions as a protein modifier via the ISGylation system.Furthermore,non-conjugated ISG15 acts as a cytokine-like protein.In this viewpoint,the interplay between free ISG15,protein ISGylation,and cytoskeletal dynamics in the tumor microenvironment is discussed.

关键词： Tumor microenvironment ISG15 ISGylation Cytoskeleton dyn

紫茎泽兰入侵前后根部蜡样芽孢杆菌含量比较及其对化感物质处理的响应

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生物安全学报 2022年第1期31卷 27-33页

作者：赵云鹏杨子豪徐刚 Esteban Rodríguez-Leyva 杨国庆扬州大学园艺与植物保护学院江苏扬州225009 posgrado en Fitosanidad Entomología y AcarologíaColegio de PostgraduadosTexcocoMexico

【目的】比较紫茎泽兰入侵地种群根部有益菌蜡样芽孢杆菌含量与原产地种群的差异,及其对化感物质的响应,为揭示紫茎泽兰与有益菌互作在入侵前后的变化及其机制提供参考。【方法】分别以紫茎泽兰原产地墨西哥的3个种群(M1、M2、M3)和入... 详细信息

【目的】比较紫茎泽兰入侵地种群根部有益菌蜡样芽孢杆菌含量与原产地种群的差异,及其对化感物质的响应,为揭示紫茎泽兰与有益菌互作在入侵前后的变化及其机制提供参考。【方法】分别以紫茎泽兰原产地墨西哥的3个种群(M1、M2、M3)和入侵地云南的3个种群(C1、C2、C3)为材料,采用同质园试验方法和蜡样芽孢杆菌分子定量的技术,检测原产地和入侵地紫茎泽兰种群植株根部(根系、根际土壤)蜡样芽孢杆菌的含量;使用不同种群紫茎泽兰植株叶片淋溶液和根系分泌液处理入侵地土壤,检测化感物质对土壤中蜡样芽孢杆菌含量的影响。【结果】紫茎泽兰入侵地种群根系蜡样芽孢杆菌含量高于原产地种群,其中入侵地种群C2的含量比原产地种群M1高1172%;在根际土壤中,2年生紫茎泽兰入侵地3个种群的蜡样芽孢杆菌含量显著高于原产地种群M1和M3。紫茎泽兰叶片淋溶液和根系分泌液处理均促进了土壤蜡样芽孢杆菌含量增加,入侵地种群叶片淋溶液处理的土壤的蜡样芽孢杆菌增加量高于原产地种群,根系分泌液处理的后期测定中也如此。【结论】与原产地相比,紫茎泽兰入侵后,根系与根际土壤蜡样芽孢杆菌含量均有所增加,增强了有益菌对入侵地种群的正反馈效应。

关键词：紫茎泽兰蜡样芽孢杆菌化感物质入侵

Lignin in storage and renewable energy applications： A review

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Journal of energy Chemistry 2018年第5期27卷 1422-1438页

作者： Jose Luis Espinoza-Acosta Patricia I. Torres-Chavez Jorge L. Olmedo-Martinez Alejandro Vega-Rios Sergio Flores-Gallardo E. Armando Zaragoza-Contreras Department of engineering and Materials Chemistry Centro de Investigacion en Materiales Avanzados Miguel de Cervantes # 120. Complejo Industrial Chihuahua Chihuahua Mexico Departamento de Investigacion y posgrado en Alimentos (DIPA) Universidad de Sonora Hermosillo Sonora Mexico

Lignin is a cheap, abundant and non-toxic group of complex phenolic polymers obtained in large amounts from the papermaking and cellulosic biofuel industries. Although the application of lignin has been ex- plored in these and several more industries, there are limited applications of lignin in the energy industry. However, numerous research revealed a great interest in the exploration of this renewable biopolymer in storage energy devices. Some of these applications include the use of lignin as an expander for lead-acid batteries, electrodes for primary and rechargeable batteries, electrodes for electronic double layer capac- itors and electrochemical pseudocapacitors, and to feed different types of fuel cells. The use of lignin in energy storage devices improves not only the performance of these devices but also decreases the price and toxicity, contributing to obtaining greener energy devices. Based on the above, this review provides an overview of the main research work related to the use of lignin as a renewable component, suitable to replace some synthetic and toxic compounds used in the fabrication of energy storage devices with particular emphasis on batteries, advanced supercapacitors, and solar and fuel cells.

关键词： Lignin Batteries Lithium-ion batteries Supercapacitors Solar cells Fuel cells

True grit: ingestion of small stone particles by hummingbirds in West Mexico

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Avian Research 2021年第4期12卷 739-749页

作者： Omar Maya-García Mauricio Ortega-Flores Jorge E.Schondube posgrado en Ciencias Biológicas Unidad de PosgradoCoordinación del Posgrado en Ciencias BiológicasUniversidad Nacional Autónoma de MéxicoEdificio D1er pisoCircuito de PosgradosCiudad UniversitariaDel.CoyoacánC.P.04510 México D.F.Mexico Laboratorio de Ecología Funcional Instituto de Investigaciones en Ecosistemas y SustentabilidadUniversidad Nacional Autónoma de MéxicoAntigua Carretera a Pátzcuaro No.8701Col.Ex Hacienda de San Joséde la HuertaC.P.58190 MoreliaMichoacánMexico

Background: Grit is used by birds mainly for grinding hard food items but can also serve a nutritional role as a source of minerals. Ingestion of grit by birds has been documented primarily in species that feed on seeds and invertebrates.Although feeding mainly on nectar and small arthropods, hummingbirds also ingest grit, but why they do so is unclear. We quantified the number of grit particles in the stomachs of six species of hummingbirds during an annual cycle in a seasonal ecosystem of West Mexico.Methods: We compared the number of grit particles in the stomachs of different hummingbird species(Mexican Violetear Colibri thalassinus, Amethyst-throated Mountaingem Lampornis amethystinus, White-eared Hummingbird Basilinna leucotis, Rivoli's Hummingbird Eugenes fulgens, Broad-tailed Hummingbird Selasphorus platycercus, and Rufous Hummingbird S. rufus), and between sex and age categories during the different seasons of a year. To deter-mine if grit was used to grind ingested arthropods, we examined the relationships between the number of grit parti-cles, the biomass of arthropods ingested, and their chitin content.Results: Although species did not differ in the number of grit particles in their stomachs, we found that grit was mostly ingested by female individuals, with only one male of one species(Mexican Violetear) presenting grit in its stomach. We also found that female hummingbirds had grit in their stomachs during the rainy and the cold-dry season(June-February) but not during the warm-dry season(March-May). Our analyses revealed no relationship between the number of grit particles and the amount of ingested arthropods and arthropod chitin content. However,high grit consumption was related to wasp ingestion on Mexican Violetears.Conclusions: Our results indicate that grit is used mainly by female hummingbirds. The seasonal variation in the ingestion of grit by female individuals suggests that it can be used to meet mineral requirements related to breed-ing;however, this topic needs further exploration. Addi

关键词： Arthropod chitin content Arthropod digestion Breeding season Mineral nutritional requirements

Historical fluxes of metal and metalloids in an aquatic ecosystem affected by land-use change and mining activities in northwestern Mexico

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International Journal of Sediment Research 2023年第5期38卷 724-738页

作者： Ochoa-Contreras Roberto Jara-Marini Martín enrique Ruiz-Fernández Ana Carolina Sanchez-Cabeza Joan Albert Meza-Figueroa Diana Pérez-Bernal Libia Hascibe posgrado en Ciencias Centro de Investigación en Alimentación y Desarrollo Unidad Hermosillo 2. Centro de Investigación en Alimentación y Desarrollo Unidad Hermosillo Unidad Acad emica Mazatlán Instituto de Ciencias del Mar y Limnología Universidad Nacional Autónoma de M exico Departamento de Geología División de Ciencias Exactas y Naturales Universidad de Sonora Rosales y Encinas

Freshwater reservoirs are essential owing because of their ecological, economic, and social importance.They are particularly vulnerable to contamination, as of metal and metalloids, derived from anthropogenic activities like mining. The temporal variations in trace element concentrations(arsenic(As),mercury(Hg), chromium(Cr), copper(Cu), nickel(Ni), lead(Pb), and zinc(Zn)), enrichment, fluxes, and possible sources were evaluated by studying two sediment cores from the La Angostura(ANG) Reservoir(northwest Mexico), using210Pb geochronology. The enrichment factors showed from null to minor enrichment for most elements, but moderate to severe enrichment of mercury(Hg). Most trace element concentrations had a detrital origin, and notable Hg concentration increases since the past decade were associated with severe drought periods, likely resulting from wildfires. The observed sediment concentrations of As and Hg can cause adverse effects on biota in the ecosystem since they are above the probable effect level(PEL). Development of strategies for metal attenuation in this reservoir is recommended and metals should be controlled until specific ecotoxicological studies are performed.

关键词： Aquatic ecosystems ~(210)Pb geochronology Mining activities Metals Metalloids enrichment factors

Biocompatibility and electrochemical evaluation of ZrO_(2) thin films deposited by reactive magnetron sputtering on MgZnCa alloy

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Journal of Magnesium and Alloys 2021年第6期9卷 2019-2038页

作者： Benjamín Millán-Ramos Daniela Morquecho-Marín Phaedra Silva-Bermudez David Ramírez-Ortega Osmary Depablos-Rivera Julieta García-López Mariana Fernández-Lizárraga JoséVictoria-Hernández Dietmar Letzig Argelia Almaguer-Flores Sandra E.Rodil Instituto de Investigaciones en Materiales Universidad Nacional Autónoma de MéxicoCDMX 04510Mexico posgrado en Ciencia e Ingeniería de Materiales Universidad Nacional Autónoma de MéxicoCDMX 04510Mexico Unidad de Ingeniería de Tejidos Terapia Celular y Medicina RegenerativaInstituto Nacional de Rehabilitación Luis Guillermo IbarraCDMX 14389Mexico posgrado en Ciencias Médicas Odontológicas y de la SaludUniversidad Nacional Autónoma de MéxicoCDMX 04510Mexico Instituto de Ciencias Aplicadas y Tecnologia Universidad Nacional Autónoma de MéxicoCDMX 04510Mexico posgrado de Doctorado en Ciencias en Biomedicina y Biotecnología Molecular Escuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalCDMX 11340Mexico Institute of Material and Process Design Helmholtz-Zentrum GeesthachtMax-Planck-Strasse 1Geesthacht 21502Germany Magnesium Innovation Centre MagIC Helmholtz-Zentrum GeesthachtMax-Planck-Strasse 1Geesthacht 21502Germany Laboratorio de Biointerfases Facultad de OdontologíaDivisión de Estudios de Posgrado e InvestigaciónUniversidad Nacional Autónoma de MéxicoCDMX 04510Mexico

Biodegradable magnesium alloys are promising candidates for temporary fracture fixation devices in orthopedics;nevertheless,its fast degradation rate at the initial stage after implantation remains as one of the main challenges to be resolved.ZrO_(2)-based coatings to reduce the degradation rate of the Mg-implants are an attractive solution since they show high biocompatibility and stability.In this work,the degradation,cytotoxicity,and antibacterial performance of ZrO_(2)thin films deposited by magnetron sputtering on a Mg-Zn-Ca alloy was evaluated.Short-term degradation of ZrO_(2)-coated and uncoated samples was assessed considering electrochemical techniques and H_(2)evolution(gas chromatography).Additionally,long term degradation was assessed by mass-loss measurements.The results showed that a 380 nm ZrO_(2)coating reduces the degradation rate and H_(2)evolution of the alloy during the initial 3 days after immersion but allows the degradation of the bare alloy for the long-term.The ZrO_(2)coating does not compromise the biocompatibility of the alloy and permits better cell adhesion and proliferation of mesenchymal stem cells directly on its surface,in comparison to the bare alloy.Finally,the ZrO_(2)coating prevents the adhesion and biofilm formation of S.aureus.

关键词： Zirconium dioxide Magnetron sputtering Short-term degradation H_(2)evolution

Ocurrence of entomopathogenic Fungus from Flea Ctenocephalides canis(Siphonaptera:Pulicidae)

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Open Journal of Veterinary Medicine 2014年第12期4卷 281-285页

作者： Jonathan E.Ortega-Palomares Hector G.Nunez-Palenius Ana M.Cruz-Avalos Aaron A.Hernandez-Rangel Roberto Lezama-Gutierrez Cesar A.Angel-Sahagun Programa Educativo de Medicina Veterinaria y Zootecnia Division Ciencias de la VidaUniversidad de GuanajuatoCampus Irapuato-SalamancaIrapuatoMexico Departamento de Agronomía Division Ciencias de la VidaUniversidad de GuanajuatoCampus Irapuato-SalamancaIrapuatoMexico Instituto Tecnologico Superior de Irapuato AbasoloMexico Posgrado en Biosciencias Division Ciencias de la VidaUniversidad de GuanajuatoCampus Irapuato-SalamancaIrapuatoMexico Facultad de Ciencias Biologicas y Agropecuarias Universidad de ColimaColimaMexico

The objective of this study was to isolate entomopathogenic fungus from fleas (Ctenocephalides canis Curtis) collected from Cannis familiaris specimens. From 60 collected fleas, two dead insects presented white mycelia growth on the body surface, changing to a creamy color as time passed;the insects presented a mummified aspect, typical characteristic caused by entomopathogenic fungus. This fungus was identified as Beauveria bassiana (Balsamo-Crivelli) Vuillemin by their mycelia characteristics, macro- and micro-morphology. Up to our knowledge, this is the first report of entomopathogenic fungus isolated from adult C. canis fleas.

关键词： Ectoparasite Biological Control Dog Beauveria bassiana

Species diversity of thrips (Thysanoptera) in selected avocado orchards from Mexico based on morphology and molecular data

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Journal of Integrative Agriculture 2018年第11期17卷 2509-2517页

作者： Daniel Bravo-Perez Ma. Teresa Santillbn-Galicia Roberto M. Johansen-Naime Hector Gonzalez-Hernandez Obdulia L. Segura-Leon Daniel L, Ochoa-Martinez Stephanie Guzman-Valencia posgrado en Fitosanidad-entomologia y Acarologia Colegio de Postgraduados Km 36.5 Carretera Mexico-Texcoco Montecillo Texcoco Estado de Mexico 56230 Mexico Instituto de Biologia de la Universidad Nacional Autonoma de Mexico (IBUNAM) Circuito exterior s/n Ciudad Universitaria Coyoacan Ciudad de Mexico 04510 Mexico posgrado en Fitosanidad-Fitopatologia Colegio de Postgraduados Km 36.5 Carretera Mexico-Texcoco Montecillo Texcoco Estado de Mexico 56230 Mexico

Avocado is one of the most important crops in the world, and Mexico is the largest producer of this fruit. Several insect pests affect its production, and thrips are amongst the most important. A key step in the design of control methods is accurate species identification. Despite this, formal reports on species diversity of thrips in Mexico are very scarce. Morphological identification can sometimes be time-consuming and inconclusive. Therefore, we explored the species diversity of thrips in Mexican avocado orchards （Michoacan state） based on partial sequences of the mitochondrial gene cytochrome oxidase subunit I （COl）. Forty-four specimens were analysed, which represented approximately 8% of all individuals collected from five localities distributed in three Municipalities. All specimens were analysed using the COl marker, and specimens within the genera Frankliniella were also analysed using a marker within the D2 domain of the 28S （28SD2） nuclear ribosomal DNA. Molecular identifications were confirmed using morphological taxonomy. Overall, six genera were found （Neohydatothrips, Scirtothrips, Frankliniella, Arorathrips, Caliothrips and Leptothrips）. AII genera contained only one species, except Frankliniella, for which there were six species. Data from the two molecular markers suggest the existence of cryptic species within Mexican F. occidentalis populations.

关键词： molecular taxonomy morphological identification cytochrome oxidase subunit I 28S nuclear ribosomal DNA.

Socio-spatial organization reveals paternity and low kinship in the Honduran white bat(Ectophylla alba)in Costa Rica

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Integrative Zoology 2021年第5期16卷 646-658页

作者： Edgar G.GUTIÉRREZ Isabela VIVAS-TORO Daniela CARMONA-RUÍZ David VILLALOBOS-CHAVES Bernal RODRÍGUEZ-HERRERA Melina Del REAL-MONROY Gloria LEÓN-AVILA Jorge ORTEGA Laboratorio de Bioconservación y Manejo Posgrado en Ciencias QuimicobiológicasDepartamento de ZoologíaEscuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalCiudad de MéxicoMéxico Department of Biology University of WashingtonSeattleWashingtonUSA Escuela de Biología Universidad de Costa RicaSan JoséCosta Rica Laboratorio de Genómica Evolutiva Doctorado en Ciencias BásicasUniversidad Autónoma de ZacatecasZacatecasZac.México Laboratorio de Genética Posgrado en Ciencias QuimicobiológicasDepartamento de ZoologíaEscuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalCiudad de MéxicoMéxico

Ectophylla alba is a tent-making bat that roosts in mixed-sex clusters comprising adults and offspring.Our goal was to determine the genetic identity of individuals belonging to different roosting groups.We tested the hypothesis of kin selection as a major force structuring group composition.We used 9 microsatellites designed for E.alba to determine the genetic identity and probability of parentage of individuals.We analyzed parentage and kinship using the software ML-Relate,GenAIEx,and Cervus.The obtained relationship probabilities(0.5)revealed a clear maternal relationship between female adults and offspring with allele compatibility,and at least 5 relationships between male adults and pups.We found a low degree of relatedness within roosting groups.Between roosting groups at different sites,the mean probability of a half-sibling relationship ranged from 0.214 to 0.244 and,for full-sibling relationship,from 0.383 to 0.553.Genetically,adult individuals were poorly related within clusters,and kinship as an evolutionary force could not explain group membership.

关键词： Ectophylla alba group composition kinship microsatellites relatedness