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Asian Agricultural Research 2023年第7期15卷 58-60,65页

作者： Lixian CUI Qingming ZENG Jinli LIU Changli pomology institute Hebei Academy of Agricultural and Forestry SciencesChangli 066600China

Changli pomology institute,Hebei Academy of Agricultural and Forestry Sciences is taken as the research *** from the relationship between party building and scientific research,in response to the problems in grassroots party building in agricultural research institutes such as insufficient theoretical learning,single party building activity mode,and lack of incentive mechanism in party affairs work,the"three integrations"approach and"four integrations"model are proposed to strengthen the deep integration of party building and scientific research in provincial-level agricultural research ***"three integrations"approach is the integrated development of party building and scientific and technological innovation,the integrated development of party building and scientific and technological services,and the integrated development of party building and technology ***"four integrations"model includes the implementation of party building innovation projects,the implementation of 1+N branch pairing and co-construction,the implementation of the"party building+"work method,and the implementation of"theme intersection"*** research aims to promote the deep integration of party building and scientific research in provincial-level agricultural research institutes.

关键词： Agricultural research institutes Party building and scientific research Deep integration Changli pomology institute

Practice and Reflection on the Science and Technology Propaganda Work of Agricultural Research institutes in the New Era : A Case Study of Changli pomology institute, Hebei Academy of Agricultural and Forestry Sciences

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Asian Agricultural Research 2023年第9期15卷 22-24页

作者： Lixian CUI Jinli LIU Qingming ZENG Zheng CHEN Changli pomology institute Hebei Academy of Agricultural and Forestry SciencesChangli 066600China Plant Protection institute Hebei Academy of Agricultural and Forestry SciencesChangli 066600China

Taking Changli Fruit Tree Research institute of Hebei Academy of Agricultural and Forestry Sciences as an example,starting from the importance of strengthening scientific and technological propaganda work in agricultural research institutes,the current situation of scientific and technological propaganda work in agricultural research institutes is analyzed in this *** response to the problems of lack of targeted evaluation mechanism,lack of distinctive publicity forms,and weak news sensitivity of science and technology propaganda workers in agricultural research institutions,strategies such as establishing a scientific and standardized evaluation system,improving incentive policies,deeply exploring propaganda content,leveraging the joint advantages of new and traditional media,and cultivating and training science and technology propaganda teams are proposed.

关键词： Agricultural research institutes Reinforcement Science and technology propaganda Changli pomology institute

Effects of 1-methylcyclopropene on skin greasiness and quality of'Yuluxiang'pear during storage at 20℃

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Journal of Integrative Agriculture 2024年第7期23卷 2476-2490页

作者： Wanting Yu Xinnan Zhang Weiwei Yan Xiaonan Sun Yang Wang Xiaohui Jia Research institute of pomology Chinese Academy of Agricultural SciencesXingcheng 125100China

During storage at 20℃,specific pear cultivars may exhibit a greasy texture and decline in quality due to fruit *** these varieties,‘Yuluxiang’is particularly susceptible to peel greasiness,resulting in significant economic ***,there is an urgent need for a preservative that can effectively inhibit the development of *** studies have demonstrated the efficacy of 1-methylcyclopropene(1-MCP)in extending the storage period of *** hypothesize that it may also influence the occurrence of postharvest peel greasiness in the‘Yuluxiang’*** this study,we treated‘Yuluxiang’pears with *** stored them at 20℃while analyzing the composition and morphology of the surface waxes,recording enzyme activities related to wax synthesis,and measuring indicators associated with fruit storage quality and physiological *** results demonstrate that prolonged storage at 20℃leads to a rapid increase in skin greasiness,consistent with the observed elevations in L^(*),greasiness score,and the content of total wax and greasy wax ***,there were indications that cuticular waxes underwent melting,resulting in the formation of an amorphous *** comparison to controls,the application of 1-MCP significantly inhibited increments in L^(*) values as well as grease scores while also reducing accumulation rates for oily waxes throughout most stages over its shelf period,additionally delaying transitions from flaky-wax structures towards their amorphous *** the initial 7 d of storage,several enzymes involved in the biosynthesis and metabolism of greasy wax components,including lipoxygenase(LOX),phospholipase D(PLD),andβ-ketoacyl-CoA synthase(KCS),exhibited an increase followed by a subsequent *** activity of LOX during early shelf life(0–7 d)and the KCS activity during middle to late shelf life(14–21 d)were significantly suppressed by ***,1-MCP effectively maintained firmness,

关键词： ‘Yuluxiang’pear skin greasiness 1-methylcyclopropene storage quality

Comparative transcriptome analysis reveals key genes and pathways in response to Alternaria alternata apple pathotype infection

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Horticultural Plant Journal 2024年第3期10卷 641-656页

作者： Kai Liu Zhaolin Liang An Yang Jiadi Yan Peihua Cong Xiaolei Han Caixia Zhang Key Laboratory of Horticultural Crop Germplasm Utilization of the Ministry of Agriculture and Rural Affairs(MOA) Research Institute of PomologyChinese Academy of Agricultural SciencesXingchengLiaoning 125100China

Apple leaf spot,caused by the Alternaria alternata apple pathotype(AAAP),is an important fungal disease of *** understand the molecular basis of resistance and pathogenesis in apple leaf spot,the transcriptomes of two apple cultivars‘Hanfu'(HF)(resistant)and‘Golden Delicious'(GD)(susceptible)were analyzed at 0,6,18,24 and 48 h after AAAP inoculation by *** each time point,a large number of significantly differentially expressed genes(DEGs)were screened between AAAP-inoculated and uninoculated apple *** of the common DEGs at four time points revealed significant differences in the resistance of‘HF'and‘GD'apple to AAAP ***,RNL,and JA signal-related genes were upregulated in both cultivars to restrict AAAP ***,genes encoding CNLs,TNLs,WRKYs,and AP2s were only activated in‘HF'as part of the resistance response,of which,some play major roles in the regulation of ET and SA signal *** analysis showed that many DEGs with opposite expression trends in the two hosts may play important regulatory roles in response to AAAP *** expression of one such gene MdERF110 in‘GD'apple leaves improved AAAP ***,this study highlights the reasons for differential resistance to AAAP infection between‘HF'and‘GD'apples which can theoretically assist the molecular breeding of disease-resistant apple crops.

关键词： Apple Transcriptome Alternaria alternata apple pathotype Infection Defense response

JrATHB-12 mediates JrMYB113 and JrMYB27 to control the anthocyanin levels in different types of red walnut

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Journal of Integrative Agriculture 2024年第8期23卷 2649-2661页

作者： Haifeng Xu Guifang Wang Xinying Ji Kun Xiang Tao Wang Meiyong Zhang Guangning Shen Rui Zhang Junpei Zhang Xin Chen Key Laboratory of Huanghuai Protected Horticulture Engineering/National Germplasm Repository of Walnut and Chestnut Shandong Institute of PomologyTai’an 271000China Research institute of Forestry Chinese Academy of ForestryBeijing 100091China College of Horticulture and Forestry Tarim UniversityAlaer 843300China

Red walnut has broad market prospects because it is richer in anthocyanins than ordinary ***,the mechanism driving anthocyanin biosynthesis in red walnut is still *** studied two types of red walnut,called red walnut 1(R1),with a red pericarp and seed coat,and red walnut 2(R2),with a red seed coat only.R1 mostly contained cyanidin-3-O-galactoside,while R2 contained a various amounts of cyanidin-3-Ogalactoside,cyanidin-3-O-arabinoside,and *** LDOX-2(LOC109007163)and LDOX-3(LOC109010746)genes,which encode leucoanthocyanidin dioxygenase/anthocyanidin synthase(LDOX/ANS),were preliminarily indicated as the crucial genes for anthocyanin biosynthesis in R1 and R2,*** MYB differential genes analysis showed that MYB27 and MYB113 are specifically expressed in the red parts of R1 and R2,respectively,and they are regarded as candidate regulatory *** expression in Arabidopsis and transient injection in walnut showed that both MYB27 and MYB113 were located in the nucleus and promoted anthocyanin accumulation,while MYB27 promoted the expression of LDOX-2,and MYB113 promoted the expression of LDOX-3and *** one-hybrid and electrophoretic mobility shift assays showed that MYB27 could only bind to the LDOX-2 promoter,while MYB113 could bind to the promoters of both LDOX-3 and *** addition,we also identified an HD-Zip transcription factor,ATHB-12,which is specifically expressed in the *** silencing the expression of ATHB-12,the R2 pericarp turned red,and MYB113 expression *** experiments showed that ATHB-12 could specifically interact with MYB113 and bind to its *** suggests that MYB27controls R1 coloration by regulating LDOX-2,while MYB113 controls R2 coloration by regulating LDOX-3 and UAGT-3,but ATHB-12 can specifically bind to and inhibit the MYB113 of the R2 pericarp so that it becomes *** study reveals the anthocyanin biosynthetic mechanisms in two differ

关键词： JrATHB-12 JrMYB113 JrMYB27 red walnut anthocyanin biosynthesis

Functions and Applications of Intestinal Symbiotic Microorganisms in Insects

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Asian Agricultural Research 2024年第8期16卷 23-28页

作者： Cong CHEN Junyu LIN Zhaoyan LIN Qianrou LI Jingyi HUANG Qijing WU Qianhua JI institute of pomology/College of Life Sciences Zhaoqing UniversityZhaoqing 526061China

Insects represent a diverse group of organisms on earth that carry a vast array of symbiotic microorganisms in their *** microorganisms have established a complex and intimate symbiotic relationship with their host insects over an extended period of evolutionary *** population structure of intestinal symbiotic microorganisms in insects exhibits a high degree of diversity,with notable differences among insect ***,within the same insect species,the population structure of symbiotic microorganisms in specific intestinal sites demonstrates a certain degree of stability and *** symbiotic microorganisms perform various physiological functions in the host *** biological functions encompass nutrient and material metabolism and immune and protective mechanisms,which have significant effects on host insect longevity,development,and reproductive *** microorganisms have diverse applications,including pest control,bioenergy production,the development of insect resources,*** advancements in high-throughput sequencing technology have revealed a growing diversity and biological properties of these *** application of these techniques has significantly contributed to the advancement of research in the field of insect intestinal microbial research,providing researchers with powerful tools for a deeper understanding and utilization of these symbiotic *** study reviewed the research progress of insect intestinal microorganisms in three aspects:diversity,biological functions,and *** objective was to provide useful references and insights for further research and applications of insect intestinal microorganisms.

关键词： Symbiotic microorganisms Intestinal flora Population structure Diversity High-throughput sequencing

Progress in Research on Insect Olfactory Perception of Habitat Odor Molecules

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Plant Diseases and Pests 2024年第3期15卷 40-45页

作者： Cong CHEN Siting WANG Jingyi DU Junyu LIN Qianrou LI Qianhua JI institute of pomology/College of Life Sciences Zhaoqing UniversityZhaoqing 526061China

A highly sensitive olfactory system allows insects to precisely identify and position volatile compounds from different sources in their habitats,and plays a crucial role in their foraging,mating,and oviposition *** evolution,insects have successfully developed a large and complex olfactory system to adapt to heterogeneous environments,enabling the maintenance of inset population.A comprehensive examination of the olfactory system of insects may therefore yield novel insights into the development of innovative pest control and prevention strategies,as well as the study of olfactory mechanisms in vertebrates and even *** paper outlines the current state of research into the signal transduction mechanism by which insects perceive the olfactory molecules of their *** aim of this review is to provide a reference point for future studies into the olfactory perception mechanism and its potential applications in pest management.

关键词： Volatile Odorant-binding protein Olfactory receptor Odorant degrading enzyme Olfactory receptor neuron

Advances in Molecular Regulatory Mechanisms of Fruit Trees under Low Temperature Stress

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Plant Diseases and Pests 2024年第3期15卷 46-48页

作者： Huan LIU Lijuan GAO Minghui JI Longfei LI Jintao XU Baofeng HAO Changli institute of pomology Hebei Academy of Agriculture and Forestry SciencesChangli 066600China

The most recent research findings on the tolerance of fruit trees to cold stress are reviewed from a molecular perspective,including the perception and transduction of low temperature calcium signaling,CBF-dependent molecular regulatory mechanisms,non-CBF-dependent molecular regulatory mechanisms,and so *** objective is to provide a reference basis for further improving the cold resistance of fruit trees and cultivating new varieties of hardy plants.

关键词： Low temperature stress Fruit trees Growth and development Molecular mechanism Research progress

A method for Improving the Efficiency of Pear Tree Crossbreeding

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Agricultural Biotechnology 2024年第2期13卷 25-26,42页

作者： Jintao XU Longfei LI Minghui JI Huan LIU Lijuan GAO Yong GUO Yingjie WANG Baofeng HAO Changli institute of pomology Hebei Academy of Agriculture and Forestry SciencesChangli 066600China

In view of the short blooming period of pear tree crossbreeding and the complexity of pollination process, a method that can improve the efficiency of crossbreeding of pear trees was provided. Meanwhile, this method can also be applied to the study of pollen xenia effect, pollination tree selection and pure pollen collection in pear tree cultivation.

关键词： Pear tree Cross breeding Efficiency Method

A Light and Simplified Branch Bending Method for Young Pear Trees

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Plant Diseases and Pests 2024年第2期15卷 19-21页

作者： Jintao XU Longfei LI Minghui JI Huan LIU Lijuan GAO Baofeng HAO Changli institute of pomology Hebei Academy of Agriculture and Forestry SciencesChangli 066600China

Aiming at high cost and low efficiency of conventional branch bending method in the modern intensive planting and labor-saving cultivation mode of young pear trees,this paper provides a new branch bending method with wide source of raw materials,cheap price and simple operation,which is also suitable for the management of low-age branches in the process of high grafting and upgrading of traditional big trees.

关键词： Pear tree Light and simplified Branch bending Method