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Effects of 1-methylcyclopropene on skin greasiness and quality of'Yuluxiang'pear during storage at 20℃

Journal of Integrative Agriculture 2024年第7期23卷 2476-2490页

作者： Wanting Yu Xinnan Zhang Weiwei Yan Xiaonan Sun Yang Wang Xiaohui Jia Research institute of pomology Chinese Academy of Agricultural SciencesXingcheng 125100China

During storage at 20℃,specific pear cultivars may exhibit a greasy texture and decline in quality due to fruit senescence.Among these varieties,‘Yuluxiang’is particularly susceptible to peel greasiness,resulting in significant economic losses.Therefore,there is an urgent need for a preservative that can effectively inhibit the development of greasiness.Previous studies have demonstrated the efficacy of 1-methylcyclopropene(1-MCP)in extending the storage period of fruits.We hypothesize that it may also influence the occurrence of postharvest peel greasiness in the‘Yuluxiang’pears.In this study,we treated‘Yuluxiang’pears with 1-MCP.We stored them at 20℃while analyzing the composition and morphology of the surface waxes,recording enzyme activities related to wax synthesis,and measuring indicators associated with fruit storage quality and physiological characteristics.The results demonstrate that prolonged storage at 20℃leads to a rapid increase in skin greasiness,consistent with the observed elevations in L*,greasiness score,and the content of total wax and greasy wax components.Moreover,there were indications that cuticular waxes underwent melting,resulting in the formation of an amorphous structure.In comparison to controls,the application of 1-MCP significantly inhibited increments in L*values as well as grease scores while also reducing accumulation rates for oily waxes throughout most stages over its shelf period,additionally delaying transitions from flaky-wax structures towards their amorphous counterparts.During the initial 7 d of storage,several enzymes involved in the biosynthesis and metabolism of greasy wax components,including lipoxygenase(LOX),phospholipase D(PLD),andβ-ketoacyl-CoA synthase(KCS),exhibited an increase followed by a subsequent decline.The activity of LOX during early shelf life(0–7 d)and the KCS activity during middle to late shelf life(14–21 d)were significantly suppressed by 1-MCP.Additionally,1-MCP effectively maintained firmness,total soluble solid(TSS)and titratable acid(TA)contents

关键词： ‘Yuluxiang’pear skin greasiness 1-methylcyclopropene storage quality

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Comparative transcriptome analysis reveals key genes and pathways in response to Alternaria alternata apple pathotype infection

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Horticultural Plant Journal 2024年第3期10卷 641-656页

作者： Kai Liu Zhaolin Liang An Yang Jiadi Yan Peihua Cong Xiaolei Han Caixia Zhang Key Laboratory of Horticultural Crop Germplasm Utilization of the Ministry of Agriculture and Rural Affairs(MOA) Research Institute of PomologyChinese Academy of Agricultural SciencesXingchengLiaoning 125100China

Apple leaf spot,caused by the Alternaria alternata apple pathotype(AAAP),is an important fungal disease of apple.To understand the molecular basis of resistance and pathogenesis in apple leaf spot,the transcriptomes of two apple cultivars‘Hanfu'(HF)(resistant)and‘Golden Delicious'(GD)(susceptible)were analyzed at 0,6,18,24 and 48 h after AAAP inoculation by RNA-Seq.At each time point,a large number of significantly differentially expressed genes(DEGs)were screened between AAAP-inoculated and uninoculated apple leaves.Analysis of the common DEGs at four time points revealed significant differences in the resistance of‘HF'and‘GD'apple to AAAP infection.RLP,RNL,and JA signal-related genes were upregulated in both cultivars to restrict AAAP development.However,genes encoding CNLs,TNLs,WRKYs,and AP2s were only activated in‘HF'as part of the resistance response,of which,some play major roles in the regulation of ET and SA signal transduction.Further analysis showed that many DEGs with opposite expression trends in the two hosts may play important regulatory roles in response to AAAP infection.Transient expression of one such gene MdERF110 in‘GD'apple leaves improved AAAP resistance.Collectively,this study highlights the reasons for differential resistance to AAAP infection between‘HF'and‘GD'apples which can theoretically assist the molecular breeding of disease-resistant apple crops.

关键词： Apple Transcriptome Alternaria alternata apple pathotype Infection Defense response

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Practice and Reflection on Strengthening Grassroots Party Building Work in Agricultural Research institutes:A Case Study of Changli pomology institute,Hebei Academy of Agricultural and Forestry Sciences

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Asian Agricultural Research 2023年第7期15卷 58-60,65页

作者： Lixian CUI Qingming ZENG Jinli LIU Changli pomology institute Hebei Academy of Agricultural and Forestry SciencesChangli 066600China

Changli pomology institute,Hebei Academy of Agricultural and Forestry Sciences is taken as the research object.Starting from the relationship between party building and scientific research,in response to the problems in grassroots party building in agricultural research institutes such as insufficient theoretical learning,single party building activity mode,and lack of incentive mechanism in party affairs work,the"three integrations"approach and"four integrations"model are proposed to strengthen the deep integration of party building and scientific research in provincial-level agricultural research institutes.The"three integrations"approach is the integrated development of party building and scientific and technological innovation,the integrated development of party building and scientific and technological services,and the integrated development of party building and technology talents.The"four integrations"model includes the implementation of party building innovation projects,the implementation of 1+N branch pairing and co-construction,the implementation of the"party building+"work method,and the implementation of"theme intersection"activities.The research aims to promote the deep integration of party building and scientific research in provincial-level agricultural research institutes.

关键词： Agricultural research institutes Party building and scientific research Deep integration Changli pomology institute

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Progress in Research on Insect Olfactory Perception of Habitat Odor Molecules

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Plant Diseases and Pests 2024年第3期15卷 40-45页

作者： Cong CHEN Siting WANG Jingyi DU Junyu LIN Qianrou LI Qianhua JI institute of pomology/College of Life Sciences Zhaoqing UniversityZhaoqing 526061China

A highly sensitive olfactory system allows insects to precisely identify and position volatile compounds from different sources in their habitats,and plays a crucial role in their foraging,mating,and oviposition activities.During evolution,insects have successfully developed a large and complex olfactory system to adapt to heterogeneous environments,enabling the maintenance of inset population.A comprehensive examination of the olfactory system of insects may therefore yield novel insights into the development of innovative pest control and prevention strategies,as well as the study of olfactory mechanisms in vertebrates and even humans.This paper outlines the current state of research into the signal transduction mechanism by which insects perceive the olfactory molecules of their habitats.The aim of this review is to provide a reference point for future studies into the olfactory perception mechanism and its potential applications in pest management.

关键词： Volatile Odorant-binding protein Olfactory receptor Odorant degrading enzyme Olfactory receptor neuron

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A method for Improving the Efficiency of Pear Tree Crossbreeding

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Agricultural Biotechnology 2024年第2期13卷 25-26,42页

作者： Jintao XU Longfei LI Minghui JI Huan LIU Lijuan GAO Yong GUO Yingjie WANG Baofeng HAO Changli institute of pomology Hebei Academy of Agriculture and Forestry SciencesChangli 066600China

In view of the short blooming period of pear tree crossbreeding and the complexity of pollination process, a method that can improve the efficiency of crossbreeding of pear trees was provided. Meanwhile, this method can also be applied to the study of pollen xenia effect, pollination tree selection and pure pollen collection in pear tree cultivation.

关键词： Pear tree Cross breeding Efficiency Method

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Advances in Molecular Regulatory Mechanisms of Fruit Trees under Low Temperature Stress

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Plant Diseases and Pests 2024年第3期15卷 46-48页

作者： Huan LIU Lijuan GAO Minghui JI Longfei LI Jintao XU Baofeng HAO Changli institute of pomology Hebei Academy of Agriculture and Forestry SciencesChangli 066600China

The most recent research findings on the tolerance of fruit trees to cold stress are reviewed from a molecular perspective,including the perception and transduction of low temperature calcium signaling,CBF-dependent molecular regulatory mechanisms,non-CBF-dependent molecular regulatory mechanisms,and so forth.The objective is to provide a reference basis for further improving the cold resistance of fruit trees and cultivating new varieties of hardy plants.

关键词： Low temperature stress Fruit trees Growth and development Molecular mechanism Research progress

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Practice and Thinking of Agricultural Science and Technology Experts Serving the Grassroots to Promote Rural Revitalization

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Asian Agricultural Research 2024年第1期16卷 44-46页

作者： Lixian CUI Jinli LIU Changli pomology institute Hebei Academy of Agricultural and Forestry SciencesChangli 066600China

Provincial agricultural research institutes are local agricultural research institutions.They are familiar with local agricultural and ru-ral conditions,and have advantages in scientific and technological achievements,technical reserves and the construction of scientific and tech-nological talent support systems.In the process of promoting the implementation of the rural revitalization strategy,all the responsibilities and obligations of the agricultural research institute are to identify the focus of scientific and technological support and talent support in rural revital-ization,and break the bottleneck and constraints of rural revitalization.By sorting out the current situation of agricultural science and technolo-gy experts serving the grassroots in the problem of talent support for rural revitalization,this paper analyzed the existing problems and put for-ward countermeasures and recommendations.

关键词： Agricultural science and technology experts Serving the grassroots Rural revitalization

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A Light and Simplified Branch Bending Method for Young Pear Trees

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Plant Diseases and Pests 2024年第2期15卷 19-21页

作者： Jintao XU Longfei LI Minghui JI Huan LIU Lijuan GAO Baofeng HAO Changli institute of pomology Hebei Academy of Agriculture and Forestry SciencesChangli 066600China

Aiming at high cost and low efficiency of conventional branch bending method in the modern intensive planting and labor-saving cultivation mode of young pear trees,this paper provides a new branch bending method with wide source of raw materials,cheap price and simple operation,which is also suitable for the management of low-age branches in the process of high grafting and upgrading of traditional big trees.

关键词： Pear tree Light and simplified Branch bending Method

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Evaluation of Cold Resistance and Semi-lethal Low Temperature(LT 50)of Nine Pear Cultivars

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Asian Agricultural Research 2024年第6期16卷 45-47页

作者： Huan LIU Lijuan GAO Jintao XU Minghui JI Longfei LI Baofeng HAO Changli institute of pomology Hebei Academy of Agriculture and Forestry SciencesChangli 066600China

[Objectives]To evaluate the cold resistance and semi-lethal temperature of pear cultivars,and provide a theoretical basis for the regional extension and breeding of cold-resistant pear cultivars.[Methods]Nine pear cultivars were used to study the changes in relative conductivity and cell injury rate of pear branches under low temperature stress,and the semi-lethal temperature(LT 50)of pear branches was analyzed by fitting Logistic equation.[Results]The relative conductivity and cell injury rate of pear branches took on the trend of slow increase,rapid increase,and slow increase the decrease of treatment temperature.The LC 50 of the nine pear cultivars were as follows:Nanguo pear-33.9℃,Wanyu-32.3℃,Red D Anjou-31.8℃,Jinfeng-31.3℃,Wujiuxiang-29.2℃,20 th Century Pear-29.1℃,Hanxiang-35.1℃,Yuluxiang-27.9℃and Korla Fragrant Pear-29.2℃.[Conclusions]The semi-lethal temperature could reflect the cold resistance of pear trees,and Wanxiang had better cold resistance.The evaluation of cold resistance and semi-lethal temperature of pear cultivars can provide theoretical basis for regional extension and breeding of cold-resistant pear cultivars.

关键词： Pear Cold resistance Semi-lethal temperature (LT 50 ) Low temperature

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High-throughput sequencing of highbush blueberry transcriptome and analysis of basic helix-loop-helix transcription factors

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Journal of Integrative Agriculture 2017年第3期16卷 591-604页

作者： SONG Yang LIU Hong-di ZHOU Qiang ZHANG Hong-jun ZHANG Zhi-dong LI Ya-dong WANG Hai-bo LIU Feng-zhi Research institute of pomology Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Germplasm Resources Utilization)Ministry of Agriculture College of Horticulture Jilin Agricultural University

The highbush blueberry（Vaccinium corymbosum）,Duke,was used to construct a de novo transcriptome sequence library and to perform data statistical analysis.Mega 4,CLC Sequence Viewer 6 software,and quantitative PCR were employed for bioinformatics and expression analyses of the basic helix-loop-helix（BHLH）transcription factors of the sequencing library.The results showed that 28.38 gigabytes of valid data were obtained from transcriptome sequencing and were assembled into 108 033 unigenes.Functional annotation showed that 32 244 unigenes were annotated into Clusters of Orthologous Groups（COG）and Gene Ontology（GO）databases,whereas the rest of the 75 789 unigenes had no matching information.By using COG and GO classification tools,sequences with annotation information were divided into 25 and 52 categories,respectively,which involved transport and metabolism,transcriptional regulation,and signal transduction.Analysis of the transcriptome library identified a total of 59 BHLH genes.Sequence analysis revealed that 55 genes of that contained a complete BHLH domain.Furthermore,phylogenetic analysis showed that BHLH genes of blueberry（Duke）could be divided into 13 sub-groups.PCR results showed that 45 genes were expressed at various developmental stages of buds,stems,leaves,flowers,and fruits,suggesting that the function of BHLH was associated with the development of different tissues and organs of blueberry,Duke.The present study would provided a foundation for further investigations on the classification and functions of the blueberry BHLH family.

关键词： blueberry bioinformatics transcdptome sequencing basic helix-loop-helix transcription factor

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