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Tea plant (Camellia sinensis) lipid metabolism pathway modulated by tea field microbe (Colletotrichum camelliae) to promote disease

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Horticulture Research 2023年第3期10卷 110-123页

作者： Shouan Liu Shuhan Zhang Shengnan He Xiaoyan Qiao A.Runa laboratory of Tea and Medicinal plant Biology College of Plant SciencesJilin UniversityChangchun 130062China laboratory of Molecular plant Pathology College of Plant SciencesJilin UniversityChangchun 130062China Guangdong Provincial Key laboratory of Tea plant Resources Innovation and Utilization Guangdong Academy of Agricultural Sciences Tea Research InstituteGuangzhou 510640China

Tea is one of the most popular healthy and non-alcoholic beverages *** anthracnose is a disease in tea mature leaves and ultimately affects yield and *** camelliae is a dominant fungal pathogen in the tea field that infects tea plants in *** pathogenic factors of fungus and the susceptible factors in the tea plant are not *** this work,we performed molecular and genetic studies to observe a cerato-platanin protein CcCp1 from ***,which played a key role in fungal pathogenicity.CcCp1 mutants lost fungal virulence and reduced the ability to produce *** and metabolome were then performed and analysed in tea-susceptible and tea-resistant cultivars,Longjing 43 and Zhongcha 108,upon *** wild-type CCA andCcCp1 infection,*** differentially expressed genes and the differentially accumulated metabolites in tea plants were clearly overrepresented such as linolenic acid and linoleic acid metabolism,glycerophospholipid metabolism,phenylalanine biosynthesis and metabolism,biosynthesis of f lavonoid,f lavone and f lavonol *** particular,the accumulation of jasmonic acid was significantly increased in the susceptible cultivar Longjing 43 upon CCA infection,in the fungal CcCp1 protein dependent manner,suggesting the compound involved in regulating fungal *** addition,other metabolites in the glycerophospholipid and phenylalanine pathway were observed in the resistant cultivar Zhongcha 108 upon fungal treatment,suggesting their potential role in defense *** together,this work indicated *** CcCp1 affected the tea plant lipid metabolism pathway to promote disease while the lost function of CcCp1 mutants altered the fungal virulence and plant response.

关键词： field metabolism Tea

Synthesis and Insecticidal Activity of Novel Camptothecin Derivatives Containing Analogs of Chrysanthemic Acid Moieties

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Journal of Integrative Agriculture 2014年第6期13卷 1320-1330页

作者： DENG Li ZHANG Lan CAO Li-dong XIE Ru-liang ZHANG Yan-ning HE Wei-zhi JIANG Hong-yun Hong-yun State Key laboratory for Biology of plant Diseases and Insect Pests Institute of plant Protection Chinese Academy of Agricultural Sciences State Key laboratory for Biology of plant Diseases and Insect Pests Institute of plant Protection Chinese Academy of Agricultural Sciences

Creating high-efifcient and environment-friendly pesticides is very important to produce the pollution free agriculture food and maintain the balance of the survival environmental of the human being. According to reports, camptothecin （CPT） and its derivatives are now being explored as a class of botanical insecticide in agriculture due to its novel mode of action. In order to improve the insecticidal activity of CPT, ten novel camptothecin （1） and 10-hydroxycamptothecin （2） derivatives （1a, 1b, 1c, 1d, 1e;2a, 2b, 2c, 2d, 2e） were designed and synthesized via esteriifcation with analogs of chrysanthemic acid, which have outstanding insecticidal activity. The results showed that compound 2a exhibited potent antifeeding effect and the best contact toxicity among the target compounds against the third-instar larvae of beet armyworm, Spodoptera exigua H＆#252;bner. Compound 2a was also found to be the most effective cytotoxic compound to the tested insect cell lines, IOZCAS-Spex-II, which were established from the fat bodies of S. exigua. It was proposed that the 10-hydroxyl group in the camptothecin derivatives is a key factor for the antifeeding activity of a compound. The nature of the substituents was considered the major factor in determining the insecticidal activity of these compounds.

关键词： camptothecin analogs of chrysanthemic acid synthesis antifeeding activity contact toxicity cytotoxicity

Relationship Between Membrane Damage Induced by Osmotic Stress and Lipid Peroxidation and Some Free Radicals

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Science China Chemistry 1993年第1期36卷 45-51页

作者：王邦锡孙莉黄久常 laboratory of plant Physiology Lanzhou University Lanzhou 730000 PRC

To treat leaf discs with solutions of various osmotic potentials of polyethylene glycols (MW 6000) by adopting the floating treatment could increase the membrane permeability, decrease the formation of malondialdehyde,and reduce the activity of peroxidase. Nevertheless, the activities of superoxide dismutase and catalase were not obviously altered. In verifying if the membrane damage was caused by lipid peroxidation initiated by active oxygen species, diethyldithiocarbamate was chosen as inhibitor superoxide dismutase,aminotriazole as catalase and mannitol as scavenger of hydroxyl free *** results have shown that there is not any correlation between lipid peroxidation and membrane ***,membrane damage caused by water stress is probably not due to free radical initiation.

关键词： lipid peroxidation free radical water stress membrane damage superoxide dismutase cucumber.

Isolation and identification of Serratia marcescens Ha1 and herbicidal activity of Ha1 ‘pesta’ granular formulation

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Journal of Integrative Agriculture 2015年第7期14卷 1348-1355页

作者： YANG Juan WANG Wei YANG Peng TAO Bu YANG Zheng ZHANG Li-hui DONG Jin-gao Mycotoxin and Molecular plant Pathology laboratory Agricultural University of Hebei

A total of 479 bacterial strains were isolated from brine （Bohai, Qinhuangdao City, Hebei Province, China）. Bioassay results indicated that 4 strains named Hal, Hal7, Ha38, and Ha384 had herbicidal activity. And strain Hal had the highest effective herbicidal activity. As a result, this study aims to JdentJfy strain Hal, characterize its physiological and biological activities, evaluate the herbicidal activity of its metabolites, and develop a ＇pesta＇ formulation and assess its effectiveness on Digitaria sanguinalis. Hal was identified as Serratia marcescens based on 16S rDNA sequencing. This strain has a flagellum, a diameter of 0.5 to 0.8 IJm, and a length of 0.9 to 2.0 IJm. The indole test shows positive results, and the catalase enzyme exhibits strong positive reactions. Results further showed that the inhibitory concentration （IC50） of the crude extracts to D. sanguinalis radicula and coleoptile were 3.332 and 2.828 mg mL-1, respectively. Both the suppression of D. sanguinalis and the cell viability of the Hal formulation in ＇pesta＇ were higher when stored at 4℃ than at （25＋2）℃. These results indi- cated that S. marcescens Hal can potentially be used as a biocontrol agent against D. sanguinalis.

关键词： Serratia marcescens bioherbicide biocontrol pesta

Isolation, Identification, and Herbicidal Activity of Metabolites Produced by Pseudomonas aeruginosa CB-4

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Journal of Integrative Agriculture 2014年第8期13卷 1719-1726页

作者： YANG Juan CAO Hong-zhe WANG Wei ZHANG Li-hui DONG Jin-gao Mycotoxin and Molecular plant Pathology laboratory Agricultural University of Hebei

CB-4, a bacterial strain with highly effective herbicidal activity, was isolated from infected corn leaves. Through morphology, physiological and biochemical tests, and 16 S ribosomal DNA gene sequencing methods, CB-4 was identified as Pseudomonas aeruginosa. We conducted activity-evaluation experiments in the laboratory to assess the herbicidal potential of metabolites produced by strain CB-4. Crude extracts of strain CB-4 have high inhibition activity on Digitaria sanguinalis. In general, the root and shoot growth parameters of D. sanguinalis were significantly reduced by metabolites of strain CB-4. The IC50 of the culture filtrate extracts for the radicula and coleoptile of D. sanguinalis were 0.299 and 0.210 mg mL-1, respectively. Component 2 of the herbicidal activity of the crude toxin from strain CB-4 was successfully purified for the first time by using high-speed counter current chromatography with a two-phase solvent system composed of petroleum ether-ethyl acetate-methanol-water（4：5：4：5, v/v） and high-performance liquid chromatography. We concluded that the metabolites of strain CB-4 have the potential to be developed as a microbe-based herbicide.

关键词： Pseudomonas aeruginosa bioherbicide biocontrol HSCCC HPLC

Heterologous expression of Lolium perenne antifreeze protein confers chilling tolerance in tomato

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Journal of Integrative Agriculture 2018年第5期17卷 1128-1136页

作者： Srinivasan Balamurugan Jayan Susan Ann Inchakalody P Varghese Shanmugaraj Bala Murugan Mani ChANDra Harish Sarma Rajeev Kumar Ramalingam Sathishkumar plant Genetic Engineering laboratory Department of BiotechnologyBharathiar University

Antifreeze proteins（AFP） are produced by certain plants, animals, fungi and bacteria that enable them to survive upon extremely low temperature. Perennial rye grass, Lolium perenne, was reported to possess AFP which protects them from cold environments. In the present investigation, we isolated AFP gene from L. perenne and expressed it in tomato plants to elucidate its role upon chilling stress. The T1 transgenic tomato lines were selected and subjected to molecular, biochemical and physiological analyses. Stable integration and transcription of Lp AFP in transgenic tomato plants was confirmed by Southern blot hybridization and RT-PCR, respectively. Physiological analyses under chilling conditions showed that the chilling stress induced physiological damage in wild type（WT） plants, while the transgenic plants remained healthy. Total sugar content increased gradually in both WT and transgenic plants throughout the chilling treatment. Interestingly, transgenic plants exhibited remarkable alterations in terms of relative water content（RWC） and electrolyte leakage index（ELI） than those of WT. RWC increased significantly by 3-fold and the electrolyte leakage was reduced by 2.6-fold in transgenic plants comparing with WT. Overall, this report proved that Lp AFP gene confers chilling tolerance in transgenic tomato plants and it could be a potential candidate to extrapolate the chilling tolerance on other chilling-sensitive food crops.

关键词： Lolium perenne antifreeze protein chilling tolerance genetic transformation transgenic tomato

Vegetation-environment relationship in Pinus wallichiana forests of the Swat Hindukush range of Pakistan

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Journal of Forestry Research 2020年第1期31卷 185-195页

作者： Inayat Ur Rahman Nasrullah Khan Kishwar Ali Siraj Ahmad laboratory of plant Ecology and Dendrochronology Department of BotanyUniversity of Malakand

Understanding the influence of environmental variables on the spatial distribution of ecological communities is essential to predict the response of vegetation to various environmental *** theory suggests that multiple environmental factors shape local species assemblages and should influence the various components of community structure and composition in different *** study aimed to classify Pinus wallichiana dominated forests in the Swat Hindukush range mountains to understand the relative influence of multiple environmental filters on its composition and *** forests represent the most typical of the species distribution in northern Pakistan and were not subjected to any phytosociological *** this purpose,thirty forest stands,spanning a wide range of physical habitats were sampled using 10 x 10 m plots and the importance value index was *** floristic and environmental data were subjected to Ward’s agglomerative cluster analysis for objective classification and ordinated with NMS ordination for pattern description and testing the vegetation-environmental *** floristically and ecologically distinct communities were recognized along the topographic gradient(elevation,r=0.377;slope,r=0.5548) coupled with soil physical(clay,r=0.2782;silt,r=0.3225) and chemical properties(pH,r=0.4975;lime,r=3982).An elevation gradient of 100 m separated the low(Pinus wallichiana-Quercus dilatata community)and middle elevations forest stands(*** pure population) from the highland population type(Pinus wallichiana-Cedrus deodara community).The floristics and structure of these forest types respond directly or indirectly to topographic and soil variables which were evidenced from the floristic composition,species richness,and community *** characteristics of the communities changed from heterogenous,dense stands to sparsely dispersed conifers,broadleaved-evergreen,and deciduous vegetation types along

关键词： Blue pine forests Classification-ordination Ecological gradient Hindukush range mountains Species composition

Effects of patch size, disturbances on diversity and structural traits of tropical semi-evergreen forest in the lowland Indo Burma hotspot: implication on conservation of the threatened tree species

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Journal of Mountain Science 2016年第8期13卷 1397-1410页

作者： Koushik MAJUMDAR Badal Kumar DATTA plant Taxonomy and Biodiversity laboratory Department of Botany Tripura University

Information on geographic distribution, population and threat status of most globally red listed species in the Indo Burma zone are inadequate. Given the increase in the prevalence of isolated forest patches in the part of this hotspot, evaluation is necessary on how size of the remnant forest patches and on-going disturbances affects structure and diversity of remaining habitats including the population of some globally threatened tree species. Quantitative vegetation inventory by 500 m long and lO m wide （0.5 ha） line transects were randomly set in nine fragmented tropical semi-evergreen forest patches in Tripura, Northeast India. The studied forests patches grouped into small, medium and large based on their respected sizes. Spatial variability was analysed in woody species diversity and structure along the patch size gradients and their response to both patch size and anthropogenic disturbances. Out of 167 species, 13 identified as red-listed species by IUCN under the present inventory. Most of the diversity and structural parameters showed statistically significant positive response with the forest patch size, except for stand dominance, disturbance, sapling density and number of aggregated distribution pattern decreased when patch size increased. In addition, most of the diversity and structural attributes showed negative response with disturbances. Present baseline data would serve as an effective tool for management and protection of this important forest type. Future habitat restoration programme and strategies for relocation of additional population and re-introduction of those globally threatened trees are recommended.

关键词： Forest diversity Semi-evergreen forest Threatened species Population composition IndoBurma zone

Development of a stable attenuated double-mutant of tobacco mosaic virus for cross-protection

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Journal of Integrative Agriculture 2024年第7期23卷 2318-2331页

作者： Xiaojie Xu Shaoyan Jiang Chunju Liu Xujie Sun Qing Zhu Xiuzhai Chen Pengchao Jiang Fenglong Wang Yanping Tian Xiangdong Li The Engineering Research Institute of Agriculture and Forestry Ludong UniversityYantai 264025China laboratory of plant virology Department of Plant PathologyCollege of Plant ProtectionShandong Agricultural UniversityTai’an 271018China Weifang Tobacco Corporation Weifang 261031China Linyi Tobacco Corporation Linyi 276000China Tobacco Research Institute Chinese Academy of Agricultural SciencesQingdao 266101China

Tobacco(Nicotiana tabacum)and tomato(Solanum lycopersicum)are two major economic crops in *** mosaic virus(TMV;genus Tobamovirus)is the most prevalent virus infecting both ***,some widely cultivated tobacco and tomato cultivars are susceptible to TMV and there is no effective strategy to control this ***-protection can be a safe and environmentally friendly strategy to prevent viral ***,stable attenuated TMV mutants are *** this study,we found that the substitutions in the replicase p126,arginine at position 196(R^(196))with aspartic acid(D),glutamic acid at position 614(E^(614))with glycine(G),serine at position 643(S^(643))with phenylalanine(F),or D at position 730(D^(730))with S,significantly reduced the virulence and replication of ***,only the mutation of S^(643) to F reduced the RNA silencing suppression activity of TMV p126.A double-mutant TMV-E614G-S643F induced no visible symptom and was genetically stable through six successive passages in tobacco ***,our results showed that TMV-E614G-S643F double-mutant could provide effective protection against the wild-type TMV infection in tobacco and tomato *** study reports a promising mild mutant for cross-protection to control TMV in tobacco and tomato plants.

关键词： cross-protection double-mutant p126 tobacco mosaic virus virulence