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Ethyl acetate fraction in ethanol extract from root of "Dai-Bai-Jie" (Marsdenia tenacissima):anti-tumor activity in A549cancer cells

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Journal of Traditional Chinese Medicine 2018年第5期38卷 668-675页

作者： Li Xiaohua Li Haitao Jin Lingyu Chen Leixi Niu Yingfeng Guan Yanhong Ma Xiaojun Zhang Lixia Key Laboratory of Dai and Southern medicine of Xishuangbanna Dai Autonomous Prefecture Yunnan Branch Institute of Medicinal PlantChinese Academy of Medical SciencesJinghong 666100China Department of Life Science and Technology China Pharmaceutical UniversityNanjing 210009China

OBJECTIVE: To evaluate the anti-tumor activity of ethyl acetate fraction(EFA), extracted with ethanol from the root of "Dai-Bai-Jie" in A549 cancer cells and its underlying ***: "Dai-Bai-Jie" was extracted with 95%ethanol-aqueous(DBJ-1), 50% ethanol-aqueous(DBJ-2), and water(DBJ-3) by reflux method. 95%ethanol-aqueous extract was separated byethyl acetate(EFA) and n-butyl alcohol(DBJ-5), consecutively. The SRB method was used to evaluate the cytotoxic activity. Annexin V-FITC staining was applied to observe the apoptosis and analyze the cell cycle activated by EFA in A549 tumor cell. Western blot was used to detect the apoptosis/related pro-teins expressions. A549 tumor cellsbearing nude mice model was employed to measure the tumor volume, mice weight, and tumor inhibition ratio in order to verify the antitumor activity in ***: DBJ-1 and EFA showed better cytotoxic activity on A549 tumor cells with IC5025 and 3.5 μg/mL, respectively. EFA can exhibit the proliferation,arrest cell cycle at G0/G1 phase, and induce apoptosis in A549 tumor cells in vitro. The mechanisms of apoptosis induced by EFA may be associated with decreasing Bcl-2 protein expression and increasing p53, Bax, Caspase-3, and Caspase-8 proteins expression. EFA also possessed significant anti-tumor efficacy in nude mice, and little toxicity was observed in the ***: EAF could induce A549 tumor cells apoptosis and G0/G1 cell cycle arrest. A549 tumor cells apoptosis induced by EAF may be associated with the decrease in the ratio of Bcl-2 and Bax mRNA levels, and increase in the expression of p53,Caspase-3, and Caspase-8 proteins.

关键词： "Dai-Bai-Jie" Antineoplastic agents Apoptosis In vitro techniques In vivo

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Chinese wheat mosaic virus: A long-term threat to wheat in China

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Journal of Integrative Agriculture 2019年第4期18卷 821-829页

作者： GUO Liu-ming HE Jing LI Jing CHEN Jian-ping ZHANG Heng-mu College of Chemistry and Life Science Zhejiang Normal University State Key Laboratory Breeding Base for Zhejiang Sustain Pest and Disease Control/MOA and Zhejiang Key Laboratory of plant Protection and Biotechnology Institute of Virology and Biotechnology Zhejiang Academy of Agricultural Sciences

In China, a soil-borne virus causing a disease of winter wheat and associated with Polymyxa graminis, has been reported for many years and is now recognized as a new species, Chinese wheat mosaic virus(CWMV). Since the determination of its genomic sequence, more progress has been made in understanding its genomic structure and functions. Molecular and serological methods have been developed to help survey the distribution of the virus and to provide the basic information needed for disease forecasting and control. At present, the best countermeasure is cultivation of resistant wheat varieties. In addition, development and application of some auxiliary countermeasures, such as rotation of non-host crops, delayed seed-sowing, reasonable application of nitrogen fertilizer, and treatment of imported seeds with fungicides before sowing, may be helpful for controlling the disease. The viral distribution and damage, virion properties, genome organization and spontaneous mutation, temperature sensitivity, and disease management options are here reviewed and/or discussed to help in developing more cost-effective countermeasures to control the disease in the future.

关键词： Chinese wheat mosaic virus(CWMV) Polymyxa graminis disease control genome structure spontaneous mutation temperature sensitivity

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Role of LOX3 Gene in Alleviating Adverse Effects of Drought and Pathogens in Rice

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Rice science 2008年第4期15卷 276-282页

作者： Nan-nan LIU[a] Ling JIANG[a] Wen-wei ZHANG[a] Ling-long LIU[a] Hu-qu ZHAI[b] Jian-min WAN[a][b] [a] State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University/Jiangsu Plant Gene Engineering Research Center Nanjing 210095 China [b] Institute of Crop sciences Chinese Academy of Agricultural Sciences Beijing 100081 China

Lipoxygenase 3 (LOX3) is a major component of the LOX isozymes in mature rice seeds. To investigate the role of LOX3 gene under stresses, a plant expression vector containing antisense cDNA of LOX3 was constructed. Rice varieties Wuyunjing 7 and Kasalath were transformed by the Agrobacterium-mediated method and transgenic rice plants were generated. PCR and Southern blot results showed that the antisense LOX3 gene was integrated into the rice genome. Analyses of embryo LOX3 deletion and semi-quantitative RT-PCR confirmed the antisense suppression of LOX3 gene in transgenic plants. The T2 antisense plants of LOX3 were sensitive to drought stress, rice blast and bacterial blight compared with non-transgenic plants. These results suggest that the LOX3 gene might function in response to stresses.

关键词： rice lipoxygenase gene antisense plant transgenic plants adverse stress drought stress rice blast bacterial blight gene function

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Identification and Characterization of Lysobacter enzymogenes as a Biological Control Agent Against Some Fungal Pathogens

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Agricultural sciences in China 2009年第1期8卷 68-75页

作者： QIAN Guo-liang HU Bai-shi JIANG Ying-hua LIU Feng-quan Department of plant Pathology College of Plant Protection Nanjing Agricultural University~Key Laboratory of Monitoring and Managementof Crop Diseases and Pest Insects Ministry of Agriculture Nanjing 210095 P.R.China

Strain OH11, a Gram-negative, nonspore forming, rod-shaped bacterium with powerful antagonistic activity, was isolated from rhizosphere of green pepper in Jiangsu Academy of Agricultural sciences of China and characterized to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain OH11 belongs to the Gammaproteobacteria and had the highest degree of sequence similarity to Lysobacter enzymogenes strain C3 （AY074793）（99%）, Lysobacter enzymogenes strain N4-7 （U89965）（99%）, Lysobacter antibioticus strain （AB019582）（97%）, and Lysobacter gummosus strain （AB16136）（97%）. Chemotaxonomic data revealed that strain OHI 1 possesses a quinine system with Q-8 as the predominant compound and C15：0 iso, C17：1 iso ω9c as the predominant iso-branched fatty acids, all of which corroborated the assignment of strain OH11 to the genus Lysobacter. Results of DNA-DNA hybridization and physiological and biochemical tests clearly showed that strain OH11 was classified as Lysobacter enzymogenes. Strain OH11 could produce protease, chitinase, and β-1,3-glucanase. It showed strong in vitro antifungal activity against Rhizoctonia solani, Sclerotinia scletotiorum, and several other phytopathogenic fungi. This is the first report of identification and characterization of Lysobacter enzymogenes as a biological control agent of plant diseases in China.

关键词： Lysobacter enzymogenes identification characterization biological control

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Techniques for detecting protein-protein interactions in living cells:principles, limitations, and recent progress

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Science China(Life sciences) 2019年第5期62卷 619-632页

作者： Yaning Cui Xi Zhang Meng Yu Yingfang Zhu Jingjing Xing Jinxing Lin Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing Forestry University College of Biological sciences & Biotechnology Beijing Forestry University Institute of plant Stress Biology State Key Laboratory of Cotton Biology Department of Biology Henan University

Detecting protein-protein interactions(PPIs) provides fundamental information for understanding biochemical processes such as the transduction of signals from one cellular location to another; however, traditional biochemical techniques cannot provide sufficient spatio-temporal information to elucidate these molecular interactions in living cells. Over the past decade, several new techniques have enabled the identification and characterization of PPIs. In this review, we summarize three main techniques for detecting PPIs in vivo, focusing on their basic principles and applications in biological studies. We place a special emphasis on their advantages and limitations, and, in particular, we introduced some uncommon new techniques, such as single-molecule FRET(smFRET), FRET-fluorescence lifetime imaging microscopy(FRET-FLIM), cytoskeleton-based assay for protein-protein interaction(CAPPI) and single-molecule protein proximity index(smPPI), highlighting recent improvements to the established techniques. We hope that this review will provide a valuable reference to enable researchers to select the most appropriate technique for detecting PPIs.

关键词： protein-protein interactions in vivo techniques RET PCA co-localization

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Restoring the plant productivity of heavy metal-contaminated soil using phosphate sludge,marble waste,and beneficial microorganisms

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Journal of Environmental sciences 2021年第1期33卷 210-221页

作者： Anas Raklami Abdel-ilah Tahiri Noura Bechtaoui ElGharmali Abdelhay Eloisa Pajuelo Marouane Baslam Abdelilah Meddich Khalid Oufdou Laboratory of Microbial Biotechnologies Agrosciencesand Enuironment(BioMAgE)Faculty of Sciences SemlaliaCadi Ayyad UniversityMarrakechMorocco Laboratory of Agri-Food Biotechnologiesand Valorization of Plant Bioresources(Agrobioval)Faculty of Sciences SemlaliaCadi Ayyad UniversityMarrakechMorocco Laboratory of Water Biodiversity and Global ChangeFaculty of Sciences SemlaliaCadi Ayyad UniversityMarrakechMorocco Department of Microbiology and Parasitology University of Seville41080 SevilleSpain Laboratory of Biochemistry Faculty of AgricultureNiigata UniversityNiigata 950-2181Japan Department of Life and Food sciences Graduate School of Science and TechnologyNigata UniversityNiigata 950-2181Japan

Assisted natural remediation(ANR)has been highlighted as a promising,less expensive,and environmentally friendly solution to remediate soil contaminated with heavy *** tested the effects of three amendments(10%compost,C;5 or 15%phosphate sludge,PS5 and PS15;and 5 or 15%marble waste,MW5 and MW15)in combination with microorganism inoculation(rhizobacteria consortium alone,mycorrhizae alone,and the two incombination)on alfalfa in contaminated *** concentrations of Zn,Cu,and Pb were measured,along with proline and malondialdehyde *** microbiological and physicochemical properties of the mining soil were *** of the amendments allowed germination and promoted *** with the rhizobacteria consortium and/or mycorrhizae stimulated plant *** and MW stimulated the production of *** of alfalfa with the rhizobacteria-mycorrhizae mixture and the application of MW allowed the safe cultivation of the legume,as shown by the low concentrations of metals in plant *** and Pb concentrations were below the limits recommended for animal grazing and accumulated essentially in *** analyses showed the positive effect of the amendments on the soil physicochemical *** treatments increased soil p H(around 7),total organic carbon,and assimilable phosphorus ***,an important decrease in soluble heavy metals concentrations was ***,our findings revealed that the applied treatments reduced the risk of metal-polluted soils limiting plant *** ANR has great potential for success in the restoration of polymetallic and acidic mining soils using the interaction between alfalfa,microorganisms,and organomineral amendments.

关键词： Bioaugmentation Bioremediation Mine soil Heavy metals Immobilization Rhizostabilization Root microbiome Wastes added-value

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PnSCR82,a small cysteine-rich secretory protein of Phytophthora nicotianae,can enhance defense responses in plants

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Journal of Integrative Agriculture 2022年第3期21卷 751-761页

作者： WANG Ya-di LI Fei ZHANG Xin LIU Ting-li LIANG Wen-xing LI De-long College of plant Health and Medicine Key Laboratory of Integrated Crop Pest Management of Shandong ProvinceQingdao Agricultural UniversityQingdao 266109P.R.China Hunan Academy of Agricultural sciences Changsha 410125P.R.China Provincial key laboratory of agrobiology Jiangsu Academy of Agricultural SciencesNanjing 210014P.R.China

A number of plant pathogenic species of Phytophthora are known to produce different classes of secretory proteins during interactions with their *** several small cysteine-rich(SCR)secretory proteins,conserved in oomycete pathogens,have been identified in Phytophthora,their specific involvement in these interactions remains *** this study,an SCR effector encoded by Pn SCR82 in *** was identified and shown to have similarities to *** phytotoxic protein,Pc F(Phytophthora cactorum Fragaria).Agroinfection with potato virus X vector,Pn SCR82,was capable of inducing plant hypersensitive cell death in Nicotiana benthamiana and Solanum ***-time PCR results indicated that transiently expressed Pn SCR82 in *** leaves activated the jasmonate,salicylic acid and ethylene signaling *** expression of Pn SCR82 enhanced plant resisitance to *** summary,our results demonstrated that *** Pn SCR82 elicits defensive responses in *** and may potentially play a significant role in future crop protection programs.

关键词： small cysteine-rich secretory protein defense responses resistance

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Prediction of candidate small non-coding RNAs inAgrobacterium by computational analysis

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The Journal of Biomedical Research 2010年第1期24卷 33-42页

作者： Tingting Zhao Ren Zhang Mingbo Wang The Laboratory Center for Basic Medical sciences Nanjing Medical University Nanjing 210029 Jiangsu Province China Department of Btologtcal sciences University of Wollongong Wollongong NSW 2522 Australia CSIRO plant Industry Canberra ACT2602 Australia

Small non-coding RNAs with important regulatory roles are not confined to eukaryotes. Recent work has uncovered a growing number of bacterial small RNAs （sRNAs）, some of which have been shown to regulate critical cellular processes. Computational approaches, in combination with molecular experiments, have played an important role in the identification of these sRNAs. At present, there is no information on the presence of small non-coding RNAs and their genes in the Agrobacterium tumefaciens genome. To identify potential sRNAs in this important bacterium, deep sequencing of the short RNA populations isolated from Agrobacterium tumefaciens C58 was carried out. From a data set of more than 10,000 short sequences, 16 candidate sRNAs have been tentatively identified based on computational analysis. All of these candidates can form stem-loop structures by RNA folding predictions and the majority of the secondary structures are rich in GC base-pairs：：Some are followed by a short stretch of U residues, indicative of a rho-independent transcription terminator, whereas some of the short RNAs are found in the stem region of the hairpin, indicative of eukaryotic-like sRNAs. Experimental strategies will need to be used to verify these candidates. The study of an expanded list of candidate sRNAs in Agrobacterium will allow a more complete understanding of the range of roles played by regulatory RNAs in prokaryotes.

关键词： small non-coding RNAs small RNAs Agrobacterium turnefaciens genome solexa sequencing technology

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Progress in the participation of Ca2+-calmodulin in heat shock signal transduction

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Progress in Natural Science:Materials International 2009年第10期19卷 1201-1208页

作者： Rengang Zhou a,b,Bing Li c,d,Hongtao Liu a,c,Daye Sun c,d,a Institute of Genetics and Physiology,Hebei Academy of Agricultural sciences,Shijiazhuang 050051,China b Hebei Provincial Key Laboratory of plant Genetic Engineering,Shijiazhuang 050051,China c Academy of Life sciences,Hebei Normal University,Shijiazhuang 050016,China d Hebei Provincial Key Laboratory of Molecular and Cell Biology,Shijiazhuang 050016,China Institute of Genetics and Physiology Hebei Academy of Agricultural Sciences Shijiazhuang 050051 China Hebei Provincial Key Laboratory of plant Genetic Engineering Shijiazhuang 050051 China Academy of Life sciences Hebei Normal University Shijiazhuang 050016 China Hebei Provincial Key Laboratory of Molecular and Cell Biology Shijiazhuang 050016 China

A novel heat shock(HS) signal transduction pathway in plants for the participation of Ca2+-calmodulin(CaM) in HS signal trans-duction was *** induces a rapid increase in intracellular free calcium ion levels([Ca2+]i),and the involvement of phospholipase Cinositol 1,4,5-trisphosphate is one of the factors leading to elevation in [Ca2+]i induced by *** also increases the expression of the CaM gene and the accumulation of the CaM *** CaM isoform,AtCaM3,in Arabidopsis is a key member in the HS signal trans-duction ***3 regulates the activity of CaM-binding protein kinase(AtCBK3) or protein phosphatase(AtPP7),promoting the activation of the HS transcription factor,AtHSFA1a,by phosphorylation/dephosphorylation and the expression of heat shock pro-tein genes,then improving heat tolerance in plants.

关键词： Ca2+ Calmodulin Protein kinase Heat shock transcription factor Signal transduction

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Landscape genetic analyses reveal host association of mitochondrial haplotypes in the Asian corn borer,Ostrinia furnacalis

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Insect Science 2021年第4期28卷 1169-1178页

作者： Zhaoke Dong Chao Li Qingqing Zhang Lili Li Zengbin Lu Fang Ouyang Yingying Song Yi Yu Xingyuan Men Institute of plant Protection Shandong Academy of Agricultural ScienceJinanChina Key Laboratory of Integrated Crop Pest Management of Shandong Province College of Plant Health and MedicineQingdao Agricultural UniversityQingdaoChina Maize Research Institute Shandong Academy of Agricultural ScienceJinanChina State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijingChina

Crop expansion often leads to high pest *** pests may have fitness trade-offs related to host use,and some host-associated genotypes may benefit and increase in ***,evidence concerning the effect of host availability on spatial distribution and frequency of mitochondrial haplotypes is *** studied genetic variation of the Asian corn borer,Ostrinia furnacalis(Guenée),across a large area during 2 years(2016 and 2017).Mitochondrial sequence data were obtained from 530 individuals collected from 79 locations in Shandong Province,*** total,155 haplotypes were found based on the combined cytochrome oxidase subunit 1(COI)and COII *** haplotypes(H2,H12,and H23)were dominant,whereas most of the other haplotypes occurred in low frequency.A haplotype network showed that the 155 haplotypes can be grouped into three *** clusters seemed to be randomly *** frequency of H12(in Cluster 1)was positively correlated with maize crop proportion,but negatively correlated with other crops(primarily vegetables,oilseed crops,and cotton)at all spatial scales(1-,3-,and 5-km radius).Cluster 2 had haplotype H23,and this cluster was negatively correlated with semi-natural *** 3 had no dominant haplotype and was not affected by landscape *** conclude that H12 may be a maize-associated *** study is needed to verify the possibility that the carriers of this haplotype may possess some fitness *** study highlights the importance of host availability in *** haplotype distribution and frequency.

关键词： agricultural landscape corn pest haplotype network host-associated haplotype mitochondrial sequence

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