检索结果-南通市图书馆

The regulatory mechanism of rapid lignification for timely anther dehiscence

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative plant Biology 2024年第8期66卷 1788-1800页

作者： Jing-Shi Xue Yi-Feng Feng Ming-Qi Zhang Qin-Lin Xu Ya-Min Xu Jun-Qin Shi Li-Fang Liu Xiao-Feng Wu Shui Wang Zhong-Nan Yang Shanghai Key Laboratory of plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200234China National Key Laboratory of plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China University of the Chinese Academy of Sciences Beijing 100049China Development center of plant Germplasm resources College of Life SciencesShanghai Normal UniversityShanghai 200234China

Anther dehiscence is a crucial event in plant reproduction,tightly regulated and dependent on the lignification of the anther *** this study,we investigated the rapid lignification process that ensures timely anther dehiscence in *** findings reveal that endothecium lignification can be divided into two distinct *** Phase Ⅰ,lignin precursors are synthesized without polymerization,while Phase Ⅱ involves simultaneous synthesis of lignin precursors and *** transcription factors MYB26,NST1/2,and ARF17 specifically regulate the pathway responsible for the synthesis and polymerization of lignin monomers in Phase Ⅱ.MYB26-NST1/2 is the key regulatory pathway responsible for endothecium lignification,while ARF17 facilitates this process by interacting with ***,our results demonstrate that the lignification of the endothecium,which occurs within approximately 26 h,is much faster than that of the vascular *** findings provide valuable insights into the regulation mechanism of rapid lignification in the endothecium,which enables timely anther dehiscence and successful pollen release during plant reproduction.

关键词： anther dehiscence ARF17 endothecium lignin MYB26 NST

Development and identification of two novel wheat-rye 6R derivative lines with adult-plant resistance to powdery mildew and high-yielding potential

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2024年第1期12卷 308-313页

作者： Guohao Han Jing Wang Hanwen Yan Tiantian Gu Lijun Cao Shiyu Liu Xiuquan Li Yilin Zhou Jieru Fan Zhipeng Shi Hong Liu Lihui Li Diaoguo An center for Agricultural resources Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuang 050021HebeiChina The National Key Facility for Crop Gene resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China The State Key Laboratory for Biology of plant Disease and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China

Powdery mildew,caused by Blumeria graminis ***(Bgt),is a devastating disease that seriously threatens wheat yield and *** control this disease,host resistance is the most effective *** with the resistance genes from common wheat,alien resistance genes can better withstand infection of this highly variable *** of elite alien germplasm resources with powdery mildew resistance and other key breeding traits is an attractive strategy in wheat *** this study,three wheat-rye germplasm lines YT4-1,YT4-2,and YT4-3 were developed through hybridization between octoploid triticale and common wheat,out of which the lines YT4-1 and YT4-2 conferred adult-plant resistance(APR)to powdery mildew while the line YT4-3 was susceptible to powdery mildew during all of its growth *** genomic in situ hybridization,multi-color fluorescence in situ hybridization,multi-color GISH,and molecular marker analysis,YT4-1,YT4-2,and YT4-3 were shown to be cytogenetically stable wheat-rye 6R addition and T1RS.1BL translocation line,6RL ditelosomic addition and T1RS.1BL translocation line,and T1RS.1BL translocation line,*** with previously reported wheat-rye derivative lines carrying chromosome 6R,YT4-1 and YT4-2 showed stable APR without undesirable pleiotropic effects on agronomic ***,these novel wheat-rye 6R derivative lines are expected to be promising bridge resources in wheat disease breeding.

关键词： Powdery mildew Secale cereale Triticum aestivum Wheat-rye 6R derivatives Agronomic performance

Salicylic acid positively regulates maize defenses against lepidopteran insects

在线全文

学校读者我要写书评

暂无评论

plant Diversity 2024年第4期46卷 519-529页

作者： Yohannes Besufekad Setotaw Jing Li Jinfeng Qi Canrong Ma Mou Zhang Cuilian Huang Lei Wang Jianqiang Wu Department of Economic plants and Biotechnology Yunnan Key Laboratory for Wild Plant ResourcesKunming Institute of BotanyChinese Academy of SciencesKunming 650201China CAS center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of plant Diversity and Specialty Crops Beijing 100093China

In response to insect attack,plants use intricate signaling pathways,including phytohormones,such as jasmonate(JA),ethylene(ET),and salicylic acid(SA),to activate ***(Zea mays)is one of the most important staple food crops around the *** studies have shown that the JA and ET signaling play important roles in maize defense against insects,but little is known about whether and how SA regulates maize resistance to insect *** this study,we ectopically expressed the NahG(salicylate hydroxylase)gene in maize plants(NahG maize)to block the accumulation of *** was found that compared with the wild-type(WT)maize,the NahG-maize exhibited decreased resistance to the generalist insects Spodoptera litura and Spodoptera frugiperda and the specialist Mythimna separata,and the compromised resistance in the NahG maize was associated with decreased levels of defensive metabolites benzoxazinoids(Bxs)and chlorogenic acid(CA).Quantification of simulated *** feedinginduced JA,JA-isoleucine conjugate(JA-Ile),and ET in the WT and NahG maize indicated that SA does not regulate JA or JA-Ile,but positively controls *** provide evidence suggesting that the SA pathway does not crosstalk with the JA or the ET signaling in regulating the accumulation of Bxs and *** analysis revealed that the bHLH,ERF,and WRKY transcription factors might be involved in SAregulated *** study uncovers a novel and important phytohormone pathway in maize defense against lepidopterous larvae.

关键词： Benzoxazinoids Salicylic acid Herbivory Maize Transcriptome

Guardian of the rice:Unveiling OsSSP1 for broad-spectrum disease resistance

在线全文

学校读者我要写书评

暂无评论

Molecular plant 2024年第7期17卷 996-998页

作者： You-Jin Lim Yong-Hwan Lee Research Institute of Agriculture and Life Sciences Seoul National UniversitySeoul08826Republic of Korea Department of Agricultural Biotechnology Seoul National UniversitySeoul 08826Republic of Korea interdisciplinary Program in Agricultural Genomics Center for Fungal Genetic ResourcesPlant Immunity Research Centerand Center for Plant Microbiome ResearchSeoul National UniversitySeoul 08826Republic of Korea

plants are continuously exposed to numerous biotic stresses throughout their *** arms race-driven coevolution with pathogens,plants have developed sophisticated immune systems,including pathogen-associated molecular pattern(PAMP)-triggered immunity(PTI)and effector-triggered immunity(ETI).PTI is initiated by the binding of danger signals to extracellular membrane-localized receptors calledpattern recognition receptors(PRRs).PTI triggers responses,including reactive oxygen species(ROS)bursts and the expression of defenseassociated ***,pathogens adapted to PTl secrete virulence proteins,effectors,to aid colonization,and ETl is then triggered by the recognition of these effectors by resistance proteins with a nucleotide-binding domain leucinerich repeat receptor(NLR).To circumvent this immune response,pathogens secrete various effectors into the host intracellular region and induce effector-triggered susceptibility(Zhou and Zhang.,2020).Currently,research on plant immunity has been primarily focused on plasma membrane and cytoplasmlocalized proteins,including oligomeric NLRs,resistosomes,and the secretion mechanisms of cytoplasmic effectors.

关键词： immunity resistance Guard

Effect of mutations on acetohydroxyacid synthase(AHAS)function in Cyperus difformis L.

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2024年第1期23卷 177-186页

作者： Xiaotong Guo Xiangju Li Zheng Li Licun Peng Jingchao Chen Haiyan Yu Hailan Cui State Key Laboratory for Biology of plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China College of resources and Environmental Sciences China Agricultural UniversityBeijing 100193China

Cyperus difformis *** a troublesome weed in paddy fields and has attracted attention due to its resistance to acetohydroxyacid synthase(AHAS)*** was found that the amino acid mutation in AHAS was the primary cause for the resistance of Cyperus ***,the effect of different mutations on AHAS function is not clear in Cyperus *** confirm the effect of mutations on AHAS function,six biotypes were collected,including Pro197Arg,Pro197Ser,Pro197Leu,Asp376Glu,Trp574Leu and wild type,from Hunan,Anhui,Jiangxi and Jiangsu provinces,China and the function of AHAS was *** AHAS in vitro inhibition assay results indicated that the mutations decreased the sensitivity of AHAS to pyrazosulfuron-ethyl,in which the I_(50)(the half maximal inhibitory concentration)of wild type AHAS was 0.04μmol L^(-1)and Asp376Glu,Pro197Leu,Pro197Arg,Pro197Ser and Trp574Leu mutations were 3.98,11.50,40.38,38.19 and 311.43μmol L^(-1),*** the determination of enzyme kinetics parameters,the Km and the maximum reaction velocity(Vmax)of the wild type were 5.18 mmol L^(-1)and 0.12 nmol mg^(-1)min^(-1),respectively,and the Km values of AHAS with Asp376Glu,Trp574Leu,Pro197Leu and Pro197Ser mutations were 0.38-0.93 times of the wild *** Km value of the Pro197Arg mutation was 1.14times of the wild type,and the Vmax values of the five mutations were 1.17-3.33-fold compared to the wild *** was found that the mutations increased the affinity of AHAS to the substrate,except for the Pro197Arg *** a concentration of 0.0032-100 mmol L^(-1)branched-chain amino acids(BCAAs),the sensitivity of the other four mutant AHAS biotypes to feedback inhibition decreased,except for the Pro197Arg *** study elucidated the effect of different mutations on AHAS function in Cyperus difformis and provided ideas for further study of resistance development.

关键词： acetohydroxyacid synthase(AHAS) mutation enzyme function Cyperus difformis

Large-scale interplant exchange of macromolecules between soybean and dodder under nutrient stresses

在线全文

学校读者我要写书评

暂无评论

plant Diversity 2024年第1期46卷 116-125页

作者： Jingxiong Zhang Shalan Li Wenxing Li Zerui Feng Shuhan Zhang Xijie Zheng Yuxing Xu Guojing Shen Man Zhao Guoyan Cao Xuna Wu Jianqiang Wu Department of Economic plants and Biotechnology Yunnan Key Laboratory for Wild Plant ResourcesKunming Institute of BotanyChinese Academy of SciencesKunming 650201China CAS center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Conservation and Utilization of Bio-resources in Yunnan and center for Life Science School of Life SciencesYunnan UniversityKunming 650500China

Parasitic plants and their hosts communicate through haustorial *** deficiency is a common stress for plants,yet little is known about whether and how host plants and parasites communicate during adaptation to such nutrient *** this study,we used transcriptomics and proteomics to analyze how soybean(Glycine max)and its parasitizing dodder(Cuscuta australis)respond to nitrate and phosphate deficiency(-N and-P).After-N and-P treatment,the soybean and dodder plants exhibited substantial changes of transcriptome and proteome,although soybean plants showed very few transcriptional responses to-P and dodder did not show any transcriptional changes to either-N ***,large-scale interplant transport of mRNAs and proteins was *** the mobile mRNAs only comprised at most 0.2%of the transcriptomes,the foreign mobile proteins could reach 6.8%of the total proteins,suggesting that proteins may be the major forms of interplant ***,the interplant mobility of macromolecules was specifically affected by the nutrient regimes and the transport of these macromolecules was very likely independently *** study provides new insight into the communication between host plants and parasites under stress conditions.

关键词： Cuscuta Mobile molecules Nutrient deficiency Host plant-parasitic plant interaction Interplant transport Systemic signaling

Knockout of a rice K5.2 gene increases Ca accumulation in the grain

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative plant Biology 2024年第2期66卷 252-264页

作者： Peitong Wang Naoki Yamaji Namiki Mitani-Ueno Jun Ge Jian Feng Ma National Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization College of Resources and Environmental SciencesNanjing Agricultural UniversityNanjing 210095China Institute of plant Science and resources Okayama UniversityKurashiki 710-0046Japan

Rice is a staple food for half of the world’s population,but it is a poor dietary source of calcium(Ca)due to the low *** is an important issue to boost Ca concentration in this grain to improve Ca deficiency risk,but the mechanisms underlying Ca accumulation are poorly ***,we obtained a rice(Oryza sativa)mutant with high shoot Ca *** mutant exhibited 26%-53% higher Ca in shoots than did wild-type rice(WT)at different Ca *** concentration in the xylem sap was 36% higher in the mutant than in the *** was no difference in agronomic traits between the WT and mutant,but the mutant showed 25% higher Ca in the polished grain compared with the ***-based cloning combined with a complementation test revealed that the mutant phenotype was caused by an 18-bp deletion of a gene,OsK5.2,belonging to the Shaker-like K+channel ***5.2 was highly expressed in the mature region of the roots and its expression in the roots was not affected by Ca levels,but upregulated by low *** showed that OsK5.2 was mainly expressed in the pericycle of the *** together,our results revealed a novel role for OsK5.2 in Ca translocation in rice,and will be a good target for Ca biofortification in rice.

关键词： accumulation biofortification calcium OsK5.2 pericycle rice translocation

Multiomics analyses of two Leonurus species illuminate leonurine biosynthesis and its evolution

在线全文

学校读者我要写书评

暂无评论

Molecular plant 2024年第1期17卷 158-177页

作者： Peng Li Meng-Xiao Yan Pan Liu Dan-Jie Yang Ze-Kun He Yun Gao Yan Jiang Yu Kong Xin Zhong Sheng Wu Jun Yang Hong-Xia Wang Yan-Bo Huang Le Wang Xiao-Ya Chen Yong-Hong Hu Qing Zhao Ping Xu shanghai Key Laboratory of plant Functional Genomics and resources Shanghai Chenshan Botanical GardenShanghai Chenshan Plant Science Research CenterChineseAcademy of SciencesShanghaiChina College of Life Sciences Shanghai Normal UniversityShanghai 200234China State Key Laboratory of plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghaiChina University of the Chinese Academy of Sciences Beijing 100049China The Research center of Chiral Drugs Innovation Research Institute of Traditional Chinese MedicineShanghai University of Traditional Chinese Medicine1200 Cailun RoadShanghai 201203China College of Chemistry and Chemical Engineering Shanghai University of Engineering ScienceShanghai 201620China

The Lamiaceae family is renowned for its terpenoid-based medicinal components,but Leonurus,which has traditional medicinal uses,stands out for its alkaloid-rich ***,the principal active compound found in Leonurus,has demonstrated promising effects in reducing blood lipids and treating ***,the biosynthetic pathway of leonurine remains largely ***,we present the chromosome-level genome sequence assemblies of Leonurus japonicus,known for its high leonurine production,and Leonurus sibiricus,characterized by very limited leonurine *** integrating genomics,RNA sequencing,metabolomics,and enzyme activity assay data,we constructed the leonurine biosynthesis pathway and identified the arginine decarboxylase(ADC),uridine diphosphate glucosyltransferase(UGT),and serine carboxypeptidase-like(SCPL)acyltransferase enzymes that catalyze key reactions in this *** analyses revealed that the UGT–SCPL gene cluster evolved by gene duplication in the ancestor of Leonurus and neofunctionalization of SCPL in ***,which contributed to the accumulation of leonurine specifically in ***,our comprehensive study illuminates leonurine biosynthesis and its evolution in Leonurus.

关键词： Lamiaceae Leonurus japonicus Leonurus sibiricus genome sequence alkaloids leonurine gene cluster biosynthetic pathway evolution

A dual-function selection system enables positive selection of multigene CRISPR mutants and negative selection of Cas9-free progeny in Arabidopsis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

aBIOTECH 2024年第2期5卷 140-150页

作者： Feng-Zhu Wang Ying Bao Zhenxiang Li Xiangyu Xiong Jian-Feng Li State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant ResourcesSchool of Life SciencesSun Yat-sen UniversityGuangzhou 510275China

The CRISPR/Cas9 technology revolutionizes targeted gene knockout in diverse organisms including ***,screening edited alleles,particularly those with multiplex editing,from herbicide-or antibiotic-resistant transgenic plants and segregating out the Cas9 transgene represent two laborious *** solutions to facilitate these processes rely on different selection ***,by taking advantage of the opposite functions of a D-amino acid oxidase(DAO)in detoxifying D-serine and in metabolizing non-toxic D-valine to a cytotoxic product,we develop a DAO-based selection system that simultaneously enables the enrichment of multigene edited alleles and elimination of Cas9-containing progeny in Arabidopsis *** five DAOs tested in Escherichia coli,the one encoded by Trigonopsis variabilis(TvDAO)could confer slightly stronger D-serine resistance than other *** expression of TvDAO in Arabidopsis allowed a clear distinction between transgenic and nontransgenic plants in both D-serine-conditioned positive selection and D-valine-conditioned negative *** a proof of concept,we combined CRISPR-induced single-strand annealing repair of a dead TvDAO with D-serine-based positive selection to help identify transgenic plants with multiplex editing,where D-serine-resistant plants exhibited considerably higher co-editing frequencies at three endogenous target genes than those selected by ***,D-valine-based negative selection successfully removed Cas9 and TvDAO transgenes from the survival offspring carrying inherited ***,this work provides a novel strategy to ease CRISPR mutant identification and Cas9 transgene elimination using a single selection marker,which promises more efficient and simplified multiplex CRISPR editing in plants.

关键词： CRISPR Genome editing D-amino acid oxidase Cas9-free Selection marker