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Phytochemical characterization of polyphenolic compounds with HPLC–DAD–ESI–MS and evaluation of lipid-lowering capacity of aqueous extracts from Saharan plant Anabasis aretioides(Coss & Moq.) in normal and streptozotocin-induced diabetic rats

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Journal of Integrative Medicine 2018年第3期16卷 185-191页

作者： Omar Farid Farid Khallouki Morad Akdad Andrea Breuer Robert Wyn Owen Mohamed Eddouks team of Physiology & Endocrine Pharmacology Faculty of Sciences and Techniques Errachidia Moulay Ismail University Division of Preventive Oncology National Center for Tumor Diseases Im Neuenheimer Feld 460 German Cancer Research Center (DKFZ)

Objective： Anabasis aretioides （Coss ＆ Moq.）, a Saharan plant belonging to Chenopodiaceae family, is widely distributed in semi-desert areas from the Tafilalet region of Morocco. This plant is extensively used by local population against diabetes and cardiovascular disorders. The purpose of the study was to investigate the effect of the aqueous A. aretioides extract on lipid metabolism in normal and strepto-zotocin （STZ）-induced diabetic rats and to identify the polyphenolic compounds present. In addition, the in vitro antioxidant activity of the aqueous A. aretioides extract was also evaluated. Methods： The effect of an aerial part aqueous extract （APAE） ofA. aretioides （5 mg/kg of lyophilized A. are-tioides APAE） on plasma lipid profile was investigated in normal and STZ-induced diabetic rats （n = 6） after once daily oral administration for 15 days. The aqueous extract was tested for its 1,1-diphenyl-2-picrylhydrazyl （DPPH） radical-scavenging activity. Polyphenolic compounds in the extracts were defini-tively characterized by high-performance liquid chromatography-diode array detection-electrospray ionization-mass spectrometry. Results： In diabetic rats, oral administration ofA. aretioides APAE provoked a significant decrease in both plasma cholesterol and triglyceride levels from the first to the second week （P 〈 0.01）. A significant decrease on plasma triglyceride levels was also observed in normal rats （P 〈 0.01）, where the reduction was 53%. In addition, the phytochemical analysis revealed the presence of 12 polyphenolic compounds. Moreover, according to the DPPH radical-scavenging activity, the aqueous extract showed an in vitro antioxidant activity. Conclusion： Aqueous A. aretioides APAE exhibits lipid-lowering and in vitro antioxidant activities. Many polyphenols were present in this extract and these phytoconstituents may be involved in the pharmaco-logical activity of this plant.

关键词： Anabasis aretioides 1,1-Diphenyl-2-picrylhydrazyl Antioxidant activity Phytochemical analysis Lipid metabolism Polyphenolic compounds

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Numerical analysis on the crack propagation and failure characteristics of rocks with double fissures under the uniaxial compression

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Petroleum 2015年第4期1卷 373-381页

作者： Leyong Chen Jianjun Liu team 141 of Sichuan Coalfield Geology Bureau Deyang618000China School of Geoscience and Technology Southwest Petroleum UniversityChengdu610500China

The fissures and rock bridges with different dips had different contributions to crack's initiation,propagation,convergence and *** this paper,based on the rock fracture theory,the crack's propagation and evolution process on rock specimen with double fissures under uniaxial compression was *** a result,the crack propagation and evolution law of rocks with different fissure dips(α=0°,15°,30°,45°,60°,75°,90°;β=45°)and different rock bridge dips(β=0°,30°,45°,60°,90°;α=45°)was obtained by numerical ***,the fissure and rock bridge dips influence on the macro mechanical properties of rock was ***,the paper investigated the influences of different fissure dips and different rock bridge dips on the bridge *** study is of great significance to reveal the impact of different dips on the mechanical mechanism of multiple-fissures rock under specific conditions,and it also has important theoretical significance for the research on multiple-fissure rock.

关键词： Double fissures rock Numerical calculation Fissure dip Dip of rock bridge Fracture theory

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The flip side of the coin:ecological function of the bee-hawking Asian hornet

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Integrative Zoology 2020年第2期15卷 156-159页

作者： Gao CHEN Zhiming ZHANG Guillaume CHOMICKI Weibang SUN Yunnan Key Laboratory for Integrative Conservation of plant Species with Extremely Small Populations KunmingChina CAS Key Laboratory for plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunmingChina Institute of Ecology and Geobotany School of Ecology and Environmental SciencesYunnan UniversityKunmingChina Department of plant Sciences University of OxfordUK The Queen’s College High StreetOxfordUK

Biological invasions around the world have experienced an unprecedented increase over the past few decades as a result of global change,worldwide trade and human mobility(Mack et al.2000).Invasive species have serious ecological and economic impacts across the *** the areas with high levels of invasion,Europe faces the greatest biological invasion(van Kleunen et al.2015).The European Union commissioned a report on invasive alien species to assess their ecological and economic ***,the Asian bee-hawking hornet Vespa velutina Lepeletier,1836 is in the line of fire in Europe(Rome&Villemant 2016).This hornet species is known as a serious predator of pollinators(such as honeybees),thus severely affecting insect pollination as well as apiculture in various European countries(Villemant et al.2011).

关键词： invasion king function

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GSA and BIGD:Filling the Gap of Bioinformatics Resource and Service in China

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Genomics, Proteomics & Bioinformatics 2017年第1期15卷 11-13页

作者： Jingchu Luo College of Life Sciences State Key Laboratory of Protein and Plant Gene Research and Center for BioinformaticsPeking UniversityBeijing 100871China

In the 2017 first issue of this Journal - Genomes, Proteomes and Bioinformatics - a special database article entitled ＂GSA： Gen- ome Sequence Archive＂ is published. This article provides a brief introduction to the platform developed by the authors from the BIG Data Center （BIGD） of Beijing Institute of Genomics （BIG）, Chinese Academy of Sciences （CAS）. The aim of the GSA project is to collect, integrate, and archive raw sequence data submitted by domestic and international users. It is one of the major activities being carried on by a team of around 50 young bioinformaticians at BIGD. In addition to the GSA system, they are also working on several bioinformatics service-orientated projects as described in one of their recent publications .

关键词： GSA and BIGD Filling the Gap of Bioinformatics Resource and Service in China

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Effect of nanosilver(nAg) on disinfection, growth, and chemical composition of young barley leaves under in vitro conditions

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Journal of Integrative Agriculture 2019年第8期18卷 1871-1881页

作者： Marcelina Krupa-Malkiewicz Jan Oszmiański Sabina Lachowicz Malgorzata Szczepanek Boguslawa Jalkiewicz Kamila Pachnowska Ireneusz Ochmian Department of plant Genetics Breeding and Biotechnology West Pomeranian University of Technology Szczecin Szczecin 71- 434 Poland Department of Fruit Vegetable and Plant Nutraceutical Technology Wroclaw University of Environmental and Life Sciences Wroclaw 51-630 Poland Department of Agrotechnology UTP University of Science and Tec hnology Bydgoszcz 85-225 Poland Departament of Cereal Crop Production Institute of Soil Science and Plant Cultivation National Research Institute Pulawy 24- 100 Poland Department of Horticulture West Pomeranian University of Technology Szczecin Szczecin 71-434 Poland

Cereals, including barley, have been playing a key role in human diet for a long time. The objective of the present study was to determine the effect of nanosilver(nAg) on limitation of infections, morphological features, and their chemical composition of young barley seedlings under in vitro condition. Addition of 0, 2, 4, 6 and 8 mg dm^–3 nAg into MS medium was *** results showed that the effect on the morphological features depended on the nAg concentration. The addition of 6 and 8 mg dm^–3 nAg into MS medium limited the number of infected barley embryos in vitro, whereas 4 and 8 mg dm^–3 nAg resulted in the highest seedlings with the longest roots. nAg in the medium affected the colour of leaves and increased the contents of chlorophyll and β-carotene, in particular in seedlings growing in MS medium supplied with 6 mg dm^–3 *** addition of 8 mg dm^–3 had the greatest effect on the contents of vitamin C and E in young barley seedlings. It was found that the contents of K and Ca in the young barley leaves were lower, as compared to control plants. The presence of 6 mg dm^–3 nAg in the medium resulted in an increased contents of N, Mg, Zn, Cu, and P. Hence, a diversified effect of nAg on individual groups of polyphenolic compounds was noticed. The presence of 2 and 8 mg dm^–3 nAg caused higher content values of polyphenolic compounds in young barley leaves.

关键词： polyphenols mineral compositions micro propagation contaminations Hordeum vulgare

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The Cellulose Synthases Are Cargo of the TPLATE Adaptor Complex

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Molecular plant 2018年第2期11卷 346-349页

作者： Clara Sanchez-Rodriguez Yanyun Shi Christopher Kesten Dongmei Zhang Gloria Sancho-Andres Alexander Ivakov Edwin R. Lampugnani Kamil Sklodowski Masaru Fujimoto Akihiko Nakano Antony Bacic Ian S. Wallace Takashi Ueda Daniel Van Damme Yihua Zhou Staffan Persson Max-Planck-lnstitute for Molecular plant Physiology Am Muehlenberg 1 14476 Potsdam Germany Department of Biology ETH Zurich 8092 Zurich Switzerland State Key Laboratory of plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China School of Biosciences University of Melbourne Parkvine 3010 VIC Australia ARC Centre of Excellence for Translational Photosynthesis Research School of Biology Australian National University GPO Box 475 Canberra ACT 2601 Australia Department of Biological Sciences Graduate School of Science The University of Tokyo Hongo Bunkyo-ku Tokyo 113-0033 Japan Live Cell Super-resolution Imaging Research team RIKEN Center for Advances Photonics Hirosawa Wako Saitama 351-0198 Japan ARC Centre of Excellence in plant Cell Walls School of Biosciences University of Melbourne Parkville 3010 VIC Australia Department of Biochemistry and Molecular Biology University of Nevada Reno NV 89557 USA Division of Cellular Dynamics National Institute for Basic Biology Okazaki Aichi 444-8585 Japan Department of plant Biotechnology and Bioinformatics Ghent University 9052 Gent Belgium Center for plant Systems Biology VIB Technologiepark 927 9052 Gent Belgium present address: Laboratory of plant Molecular Genetics Graduate School of Agricultural and Life Sciences The University of Tokyo 1-1-1 Yayoi Bunkyo-ku Tokyo 113-8657 Japan

Dear Editor Clathrin-mediated endocytosis （CME） is an evolutionary conserved mechanism by which plasma membrane （PM）-based cargo proteins are recognized by adaptor protein complexes and internalized. Apart from the canonical adaptor complex, AP-2, plant cells rely on the TPLATE complex （TPC） to execute CME （Gadeyne et al., 2014）. FT160100218, DP110100410）, the Ministry of Education, Culture, Sports, Science, and Technology of Japan （24114003, 15H04382, and 17K19412）, the European Research Council （ERC grant 682436）, the IRRTF-RNC （no. 501892） and a USA National Science Foundation CAREER Award.

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Does pollinator dependence decrease along elevational gradients?

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plant Diversity 2023年第4期45卷 446-455页

作者： Yue-Wen Xu Lu Sun Rong Ma Yong-Qian Gao Hang Sun Bo Song CAS Key Laboratory for plant Diversity and Biogeography of East Asia/Yunnan Key Laboratory for Integrative Conservation of plant Species with Extremely Small Populations Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China College of Life Sciences Shaanxi Normal UniversityXi'an 710119China Yunnan Forestry Technological College Kunming 650224China

plants have long been thought to be less dependent on pollinators for seed production at higher elevations due to adverse pollination ***,recent research has yet to consistently support the generality of this *** this study,we asked whether pollinator dependence decreases along an elevational gradient and how it varies with various reproductive *** answer these questions,we quantified pollinator-plant associations and various reproductive traits for 112 flowering plants spanning a large elevational gradient(990-4260 m a.s.l.)in the Qinghai-Tibet *** found that flowering plants in the Qinghai-Tibet Plateau region are highly dependent on pollinators for seed production(76.2%of seed production was contributed by animal pollinators and 44.6%of plants would produce no seed without pollinator visitation).Contrary to our expectation,there was no significant elevational gradient in pollinator dependence *** the pollinator dependence index was not significantly correlated with pollen limitation,flower size,floral longevity,or reward type,it was correlated with compatibility status and flowering *** findings indicate that pollinator dependence does not decrease along an elevational gradient in the Qinghai-Tibet *** study also highlights the severe vulnerability of flowering plant seed production to pollinator declines under global change in the Qinghai-Tibet Plateau region,particularly for early-flowering or self-incompatible plants growing at higher elevations(e.g.,subnival belt).

关键词： Global change Pollen limitation Pollinator decline Qinghai-Tibet plateau Seed production

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Seed dormancy,germination and storage behavior of Magnolia sinica,a plant species with extremely small populations of Magnoliaceae

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plant Diversity 2022年第1期44卷 94-100页

作者： Liang Lin Lei Cai Lei Fan Jun-Chao Ma Xiang-Yun Yang Xiao-Jian Hu The Germplasm Bank of Wild Species Kunming Institute of BotanyChinese Academy of SciencesKunming650201YunnanChina Yunnan Key Laboratory for Integrative Conservation of plant Species with Extremely Small Populations Key Laboratory far Plant Diversity and Biogeography of East AsiaKunming Institute of BotanyChinese Academy of SciencesKunming650201YunnanChina

Magnolia sinica is one of the most endangered Magnoliaceae species in *** biology information concerning its long-term ex situ conservation and utilization is *** study investigated dormancy status,germination requirements and storage behavior of *** matured seeds germinated to ca.86.5%at 25/15℃but poorly at 30℃;GA3 and moist chilling promoted germination significantly at 20℃.Embryos grew at temperatures(alternating or constant)between 20℃and 25℃,but not at 5℃or 30℃.Our results indicate that *** seeds possibly have non-deep simple mor-phophysiological dormancy(MPD).Seeds survived desiccation to 9.27%and 4.85%moisture content(MC)as well as a further 6-month storage at-20℃and in liquid nitrogen,including recovery in vitro as excised *** established protocol ensured that at least 58%of seedlings were obtained after both cold storage and *** results indicate that both conventional seed banking and cryopreservation have potential as long-term ex situ conservation methods,although further optimized approaches are recommended for this critically endangered magnolia species.

关键词： Magnolia sinica Seed Dormancy Storage behavior Cryopreservation Excised embryo

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RNA Quality Control as a Key to Suppressing R Silencing of Endogenous Genes in plants

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Molecular plant 2016年第6期9卷 826-836页

作者： Lin Liu Xuemei Chen Guangdong Provincial Key Laboratory for plant Epigenetics College of Life Sciences and Oceanography Shenzhen University Shenzhen 518060 P.R. China Department of Botany and plant Sciences Institute of Integrative Genome Biology University of California Riverside CA 92521 USA Howard Hughes Medical Institute University of California Riverside CA 92521 USA

RNA quality control of endogenous RNAs is an integral part of eukaryotic gene expression and often relies on exonucleolytic degradation to eliminate dysfunctional transcripts. In parallel, exogenous and selected endogenous RNAs are degraded through RNA silencing, which is a genome defense mechanism used by many eukaryotes. In plants, RNA silencing is triggered by the production of double-stranded RNAs （dsRNAs） by RNA-DEPENDENT RNA POLYMERASEs （RDRs） and proceeds through small interfering （si） RNA-directed, ARGONAUTE （AGO）-mediated cleavage of homologous transcripts. Many studies revealed that plants avert inappropriate posttranscriptional gene silencing of endogenous coding genes by using RNA surveillance mechanisms as a safeguard to protect their transcriptome profiles. The tug of war between RNA surveillance and RNA silencing ensures the appropriate partitioning of endogenous RNA substrates among these degradation pathways. Here we review recent advances on RNA quality control and its role in the suppression of RNA silencing at endogenous genes and discuss the mechanisms underlying the crosstalk among these pathways.

关键词： gene silencing RNA quality control aberrant RNA siRNA

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Genome-wide association analysis identified molecular markers and candidate genes for flower traits in Chinese orchid (Cymbidium sinense)

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Horticulture Research 2023年第11期10卷 193-207页

作者： Fengxi Yang Yudi Guo Jie Li Chuqiao Lu Yonglu Wei Jie Gao Qi Xie Jianpeng Jin Genfa Zhu Guangdong Key Laboratory of Ornamental plant Germplasm Innovation and Utilization Environmental Horticulture Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510640China

The orchid,the champagne of f lowers,brings luxury,elegance,and novelty to *** sinense is a symbol of gigantic floral variability on account ofwavering shapes and sizes of f loral organs,althoughmarker-trait association(MTA)has not been studied for its f loral *** evaluated markers associated with 14 f loral traits of *** through a genome-wide association study(GWAS)of 195 accessions.A total of 65318522 single-nucleotide polymorphisms(SNPs)and 3906176 insertion/deletion(InDel)events were identified through *** these,4694 potential SNPs and 477 InDelswere identified as MTAs at−log10 P>*** genes related to these SNPs and InDels were largely associated with f loral regulators,hormonal pathways,cell division,and metabolism,playing essential roles in tailoring f loral ***,20 candidate SNPs/InDels linked to 11 genes were verified,8 of which were situated on exons,onewas located in the 5-UTR and twowere positioned in ***,themultitepal trait-related gene RABBIT EARS(RBE)was found to be the most crucial *** analyzed the role of CsRBE in the regulation of flower-related genes via efficient transient overexpression in *** protoplasts,and found that the floral homeotic genes CsAP3 and CsPI,as well as organ boundary regulators,including CsCUC and CsTCP genes,were regulated by ***,we obtained key gene loci for important ornamental traits of orchids using genome-wide association analysis of populations with natural *** findings of this study can do a great deal to expedite orchid breeding programs for shape variability.

关键词： traits analysis breeding

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