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Journal of Integrative plant Biology 2015年第7期57卷 618-627页

作者： Christine Bttcher Crista A.Burbidge Valentina di Rienzo Paul K.Boss Christopher Davies CSIRO Agriculture Flagship Glen Osmond Department of Soil Plant and Food SciencesSection of Genetics and Plant BreedingUniversity of Bari "Aldo Moro"

The plant hormone jasmonic acid （JA） is essential for stress responses and the formation of reproductive organs, but its role in fruit development and ripening is unclear. Conjugation of JA to isoleucine is a crucial step in the JA signaling pathway since only JA-Ile is recognized by the jasmonate receptor. The conjugation reaction is catalyzed by JA-amido synthetases, belonging to the family of Gretchen Hagen3 （GH3） proteins. Here, in vitro studies of two grapevine （Vitis vinifera L. cv Shiraz） GH3 enzymes, VvGH3-7 and VvGH3-9, demonstrated JA-conjugating activ- ities with an overlapping range of amino acid substrates, including isoleucine. Expression studies of the correspond- ing genes in grape berries combined with JA and JA-lle measurements suggested a primary role for JA signaling in fruit set and cell division and did not support an involvement of JA in the ripening process. In response to methyl JA （MeJA） treatment, and in wounded and unwounded （distal）leaves, VvGH3-9 transcripts accumulated, indicating a participation in the JA response. In contrast, VvGH3-7 was unresponsive to MeJA and local wounding, demonstrating a differential transcriptional regulation of VvGH3-7 and VvGH3- 9. The transient induction of VvGH3-7 in unwounded, distal leaves was suggestive of the involvement of an unknown mobile wound signal.

关键词： Berry development GH3 grapevine jasmonic acid wound response

SRG1,Encoding a Kinesin-4 Protein,Is an Important Factor for Determining Grain Shape in Rice

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Rice science 2018年第6期25卷 297-307页

作者： QIN Ran Delara AKHTER YANG Chengcong Ujjal Kumar NATH Jamal ESHAG JIN Xiaoli SHI Chunhai Department of Agronomy Zhejiang University Department of genetics and plant breeding Sylhet Agricultural University Department of genetics and plant breeding Bangladesh Agricultural University

Grain shape is one of the important agronomic traits, which is closely related to the yield of rice.A new rice mutant, named small and round grain(srg1), was isolated from an indica cultivar Zhenong 34 by ethyl methane sulfonate(EMS) mutagenesis. The microscopic analysis showed that the cell length of spikelet in srg1 was decreased compared with that in wild type(WT), which caused the grain length ***, the grains of srg1 were wider than those of WT because of the increased cell layers in spikelet in the lateral direction. Therefore, the inhibition of cell expansion and increased cell proliferation collectively led to the small and round grain. By map-based cloning, the gene SRG1 was located on the short arm of chromosome 9, which encodes a kinesin-4 protein, represented by the gene LOC_Os09 g02650. A single nucleotide polymorphism, occurred in the 16 th exon of SRG1, led to premature translation stop in mutant. The cell cycle-related genes were up-regulated in srg1, which conferred that SRG1 controlled grain width through the cell proliferation. Since the role of SRG1 in regulating grain shape was not clarified well before, it is valuable to explore the mechanism of grain development. This study could, hence, provide a morphogenesis and molecular basis for elucidating the function of SRG1, as well as a new germplasm resource for the further study of grain development.

关键词： small and round grain kinesin-4 protein gene mapping rice

Phylogenetic relationships among Hystrix species and related species based on expressed sequence tag-polymerase chain reaction

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Journal of Systematics and Evolution 2011年第1期49卷 65-71页

作者： Hisashi TSUJIMOTO Laboratory of plant genetics and breeding Science Faculty of Agriculture Tottori University

Twelve species, including three Hystrix species, five Leymus species, Hordeum bogdanii, Pseudoroegneria spicata, Psathyrostachys huashanica, and Roegneria ciliaris, were used for expressed sequence tag-polymerase chain reaction (EST-PCR) assay. A total of 125 products were amplified by 72 sets of EST-PCR markers developed in barley, among which 106 (84.8%) products were found to be polymorphic. Each EST-PCR marker produced 0–6 polymorphic bands, with an average of 1.47. The relationship between H. duthiei ssp. duthiei and H. duthiei ssp. longearistata is close, but they are remote to H. patula. Hystrix duthiei ssp. duthiei and H. duthiei ssp. longearistata were clustered with Leymus species and Psathyrostachys huashanica, which suggested that they have close genetic relationships. The results of EST-PCR analysis are basically comparable with those obtained from studies on cytology, which indicated that EST-PCR can be used to assess the genetic relationships among the perennial species in Triticeae.

关键词： cluster analysis expressed sequence tag Hystrix molecular marker phylogenetic relationship.

Antixenosis and Tolerance of Rice Genotypes Against Brown planthopper

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Rice science 2016年第2期23卷 96-103页

作者： Preetinder Singh SARAO Jagadaish Sanmallappa BENTUR Rice section Department of Plant Breeding and Genetics Punjab Agricultural University Ludhiana 141004 India Indian Institute of Rice Research Hyderabad 500030 India

Nine genotypes were evaluated under greenhouse conditions for antixenosis and tolerance against brown planthopper （BPH, Nilaparvata lugens Stal）. In antixenosis studies, proportion of insects settled on a test genotype in relation to the susceptible control TN1 was recorded, with significantly lower proportion of nymphs （55.22%-59.18%）, adult males （60.33%-60.75%）, and adult females （80.56%- 79.26%） settled on RP2068-18-3-5 and Ptb33 in relation to those on TN1. Based on number of feeding sites, the test genotypes were ranked in order from the highest to the lowest as RP2068-18-3-5, Ptb33, MR1523, Rathu Heenati, Sinnasivappu, ARC10550, MO1, INRC3021 and TNI. The order was exactly reverse in terms of fecundity expressed as number of eggs laid per female. In tolerance studies, days to wilt, functional plant loss index and plant dry weight loss to BPH dry weight produced were recorded. RP2068-18-3-5, Rathu Heenati and Ptb33 performed better than the other test genotypes. These results helped in relative quantification of BPH resistance levels in the genotypes. RP2068-18-3-5, a new effective source of BPH resistance, can be used in resistance breeding after tagging of resistant genes/QTLs linked to different parameters of antixenosis and tolerance with selectable molecular markers.

关键词： antixenosis molecular marker Nilaparvata lugens resistance breeding rice tolerance

Development and transferability of two multiplexes nSSR in Scots pine(Pinus sylvestris L.)

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Journal of Forestry Research 2015年第2期26卷 361-368页

作者： Stefana Ganea Sonali S.Ranade David Hall Sara Abrahamsson Maria Rosario Garcia-Gil Department of Forest genetics and plant Physiology SLU901 83 UmeaSweden Department of genetics and plant breeding USAMV400372 Cluj-NapocaRomania

Single sequence repeat（SSR） multiplexing is a semi high-throughput PCR methodology for the analysis of multiple *** developed two SSR multiplexes selected from SSR loci previously reported in the pine literature and tested the transferability of both SSR multiplexes in nine other pine *** tested 234 nuclear SSR loci（n SSRs） previously described in the pine literature and selected ten n SSRs following the simple criteria of interpretability and *** nuclear loci were divided into two n SSRs multiplex sets and their amplification was optimized for three different multiplex PCR methods based on：（a） a custom PCR protocol,（b） a custom protocol with hotstart taq polymerase,and（c） a commercially available kit for SSR *** validate their performance,the level of genetic diversity was assessed in three Scots pine natural populations（Hungary,northern Sweden and southern Sweden）.In addition,we also tested the transferability of these multiplexes in nine other pine *** have developed two n SSRs multiplexes of five loci each that will contribute to reduce the costs of n SSR scoring,while increasing the capacity of n SSR loci *** was successful in all three populations（94 % success） and the level of polymorphism（7.1 alleles/marker） was similar to that previously reported for other Scots pine natural *** of both multiplexes was successful for those pine species closely related to Scots pine.

关键词： Genetic diversity Nuclear SSR SSR multiplex Scots pine

Laboratory-and Field-Phenotyping for Drought Stress Tolerance and Diversity Study in Lentil (Lens culinaris Medik.)

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Phyton-International Journal of Experimental Botany 2021年第3期90卷 949-970页

作者： Shahida Akter Israt Jahan MdAmir Hossain Mohammad Anwar Hossain Department of genetics and plant breeding Bangladesh Agricultural UniversityMymensingh2202Bangladesh

Drought susceptibility and low genetic variability are the major constraints of lentil(Lens culinaris Medik.)production *** of an efficient pre-field drought phenotyping technique and identification of diversified drought tolerant lentil genotype(s)are therefore vital and *** separate experiments were conducted using thirty diverse lentil genotypes to isolate drought tolerant genotype(s)as well as to assess their *** both of the experiments,significant(p≤0.01)variation in genotype(G),treatment(T)and G X T was observed for most of the studied *** experiment I,genotypes were examined for drought tolerance at the seedlings stage under hydroponic conditions by assessing root and shoot *** the 30 genotypes studied,BM-1247,BM-1227 and BM-502 were selected as highly tolerant to drought stress as they showed maximum seedling survivability and minimum reduction in growth parameters under drought *** experiment II,the genotypes were assayed for diversity and drought stress tolerance based on morphological traits grown under field *** stress caused a substantial reduction in yield attributing traits,however,the genotypes BM-1247,BM-981,BM-1227 and BM-502 were categorized as drought tolerant genotypes with less than 20%yield *** field screening result of drought stress tolerance was coincided well with the results of laboratory *** divergence study reflected the presence of considerable diversity among the *** laboratory and field screening results,the genotypes,BM-1247,BM-1227,BM-981 and BM-502 were selected as the best drought tolerant *** information can be exploited for further breeding in developing drought tolerance in lentil.

关键词： Lentil water stress seedling survival yield attributing traits phenotypic diversity

Identification and fine mapping of quantitative trait loci for the number of vascular bundle in maize stem

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Journal of Integrative plant Biology 2016年第1期58卷 81-90页

作者： Cheng Huang Qiuyue Chen Guanghui Xu Dingyi Xu Jinge Tian Feng Tian Department of plant genetics and breeding National Maize Improvement Center of China China Agricultural University

Studies that investigated the genetic basis of source and sink related traits have been widely ***, the vascular system that links source and sink received much less attention. When maize was domesticated from its wild ancestor, teosinte, the external morphology has changed dramatically; however, less is known for the internal anatomy changes. In this study, using a large maize-teosinte experimental population, we performed a high-resolution quantitative trait locus（QTL） mapping for the number of vascular bundle in the uppermost internode of maize *** results showed that vascular bundle number is dominated by a large number of small-effect QTLs, in which a total of 16 QTLs that jointly accounts for 52.2% of phenotypic variation were detected, with no single QTL explaining more than 6% of variation. Different from QTLs for typical domestication traits, QTLs for vascular bundle number might not be under directional selection following *** Near Isogenic Lines（NILs） developed from heterogeneous inbred family（HIF）, we further validated the effect of one QTL qVb9-2 on chromosome 9 and fine mapped the QTL to a 1.8-Mb physical region. This study provides important insights for the genetic architecture of vascular bundle number in maize stem and sets basis for cloning of qVb9-2.

关键词： Fine-mapping genetic architecture maize quantitativetrait locus vascular bundle number

breeding Potential of Some Exotic Tomato Lines:A Combined Study of Morphological Variability,Genetic Divergence,and Association of Traits

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Phyton-International Journal of Experimental Botany 2022年第1期91卷 97-114页

作者： Shafiul Islam Lutful Hassan Mohammad Anwar Hossain Department of genetics and plant breeding Bangladesh Agricultural UniversityMymensingh2202Bangladesh

Tomato(Solanum lycopersicum L.)is called‘the poor man’s orange’due to its low price and improved nutritional *** experiment was conducted to study the breeding potential of some exotic tomato lines by assessing various qualitative and quantitative traits conferring yield and quality *** the qualitative traits,greater variability was observed for growth type,stem hairiness,and fruit shape and size.A determinate growth habit was observed in the genotype AVTO9802 while the genotype AVTO0102 produced yellow color fruits.A significant(p≤0.01)variation was also observed for the studied quantitative *** on yield and traits attributed to yield,the genotypes AVTO0314,GPB0107,GPB0120 and AVTO9802 were selected as promising *** differences between the genotypic and phenotypic coefficients of variation(GCV and PCV)of the studied quantitative traits were very *** suggests that the apparent variation was mainly due to the *** higher GCVand PCV values were observed for the number of primary branches plant^(−1)(NPB),number of fruits cluster^(−1)(NFC),individual fruit weight(IFW)and total soluble solids(TSS).High heritability was recorded for all quantitative traits in a broad ***,the individual fruit diameter showed the highest heritability(99.56).The highest(102.75)genetic advance(GA)was observed for the number of fruits plant^(−1)(NFP).High heritability coupled with high GA as percentage of mean were recorded for the traits NFP,NFC,fruit yield plant^(−1)(FYP)and *** showed a significant positive correlation with NFC(0.714^(***))and a negative correlation with days to the first harvest(−0.539^(***))and plant height(−0.492**).Principal component analysis revealed that the first four components explained 78.5%of the total variation among the ***,the promising genotypes(AVTO0314,GPB0107,GPB0120,AVTO9802 and AVTO0102)isolated from this study can be used for developing high-yielding and high-quality tomato va

关键词： Tomato yield and quality traits genetic advance heritability correlation coefficient principal component analysis

Molecular mapping of QTLs for root response to phosphorus deficiency at seedling stage in wheat (Triticum aestivum L.)

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Progress in Natural Science:Materials International 2007年第10期17卷 1177-1184页

作者： Li Zhenxing, Ni Zhongfu, Peng Huiru, Liu Zhiyong, Nie Xiuling, Xu Shengbao, Liu Gang and Sun Qixin(Key Laboratory of Crop Heterosis and Utilization and State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Genomics and Genetic Improvement, Beijing Key Laboratory of Crop Genetic Improvement, Department of plant genetics & breeding, China Agricultural University, Beijing 100094, China) Key Laboratory of Crop Heterosis and Utilization Department of Plant Genetics and Breeding China Agricultural University Beijing 100094 China Key Laboratory of Crop Genomics and Genetic Improvement Department of Plant Genetics and Breeding China Agricultural University Beijing 100094 China Beijing Key Laboratory of Crop Genetic Improvement Department of Plant Genetics and Breeding China Agricultural University Beijing 100094 China

Phosphorus (P) deficiency in the soil is one of the major abiotic stresses that limit plant growth and crop productivity throughout the world. Development of cultivars with improved P-deficiency tolerance is an efficient strategy for sustainable agriculture. plant roots play an important role in crop growth and development, especially in nutrient uptake and improvement of P-efficiency. Mapping quantitative trait loci (QTLs) for root traits and their response to low P stress at seedling stage will facilitate the development of P-efficient wheat cultivars. In this study, 30 QTLs (LOD>2.0) were mapped for the three root traits, such as root length, root number and root dry matter under different P supply conditions and their response to P-stress. These QTLs were distributed on 14 chromosomes, with each of the 5 QTLs explaining more than 10% phenotype variance. Analyses showed that root traits and their response to P-deficiency were controlled by different QTLs. In addition, alleles with positive effects were separated on both parents, and wheat cultivars with improved P-efficiency could be developed by accumulating these positive effect alleles together.

关键词： QTLs roots wheat seedling RIL P-deficiency.

Proteomic Profiling and Protein-Protein Interaction Network Reveal the Molecular Mechanisms of Susceptibility to Drought Stress in Canola(Brassica napus L.)

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Phyton-International Journal of Experimental Botany 2022年第7期91卷 1403-1417页

作者： Reza Shokri-Gharelo Ali Bandehagh Mohammad Anwar Hossain Department of plant breeding and Biotechnology University of TabrizTabriz51368Iran Department of genetics and plant breeding Bangladesh Agricultural UniversityMymensingh2202Bangladesh

Drought stress is one of the most important abiotic stresses that plants face frequently in *** drought conditions,many morphological,physiological,and molecular aspects of plants are changed and as a result plants experience a remarkable reduction in growth,yield,and *** expand our understanding of the molecular basis of the plant response to drought stress,the proteomic profile and protein-protein network of canola(Brassica napus L.)were *** focus was to show molecular mechanisms related to canola susceptibility to drought *** experiment used a completely randomized design,implemented in a hydroponic system under greenhouse *** impose drought stress,plants were exposed to Hoagland’s solution supplemented with polyethylene glycol(PEG)6000 for 7 *** drought stress resulted in 161reproducible protein spots in twodimensional electrophoresis of canola *** t-student test showed 21 differentially abundant proteins(DAP),of which 2 and 19 were up and down accumulated,*** spots identified as 1-aminocyclopropane-1-carboxylate oxidase and D-2-hydroxyglutarate dehydrogenase showed an increased abundance of 2.11 and 1.77,*** extended protein-protein interaction of differentially abundant proteins and KEGG analysis showed 47 pathways directly and indirectly associated with canola response to drought *** with increased abundance were associated with amino acid and signaling processes,whereas DAPs with decreased abundance were mostly connected with pathways responsible for energy *** results of the study will help to elucidate further the molecular events associated with the susceptibility to drought stress in canola.

关键词： Abiotic stress differentially abundant protein molecular function proteomics susceptibility