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Recognition of H3K9me1 by maize RNA-directed DNA methylation factor SHH2

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Journal of Integrative plant Biology 2021年第6期63卷 1091-1096页

作者： Yuhua Wang Xuelin Zhou Jinyan Luo Suhui Lv Rui Liu Xuan Du Bei Jia Fengtong Yuan Heng Zhang Jiamu Du National Key Laboratory of plant Molecular Genetics and Shanghai Center for plant Stress Biology Center for Excellence in Molecular Plant SciencesThe Chinese Academy of SciencesShanghai 201602China University of Chinese Academy of Sciences Beijing 100049China School of Life Science Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education InstitutesInstitute of Plant and Food ScienceSchool of Life SciencesSouthern University of Science and TechnologyShenzhen 518055China

RNA-directed DNA methylation(Rd DM) is a plant-specific de novo DNA methylation pathway,which has extensive cross-talk with histone modifications. Here, we report that the maize RdDM regulator SAWADEE HOMEODOMAIN HOMOLOG 2(SHH2) is an H3 K9 me1 reader. Our structural studies reveal that H3 K9 me1 recognition is achieved by recognition of the methyl group via a classic aromatic cage and hydrogen-bonding and salt-bridge interactions with the free protons of the mono-methyllysine. The di-and tri-methylation states disrupt the polar interactions, decreasing the binding affinity. Our study reveals a monomethyllysine recognition mechanism which potentially links RdDM to H3 K9 me1 in maize.

关键词： epigenetics H3K9me1 maize RNA-directed DNA methylation SAWADEE domain SHH2

A rapid method to quantify fungicide sensitivity in the wheat stripe rust pathogen Puccinia striiformis ***

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A rapid method to quantify fungicide sensitivity in the whea...

中国植物病理学会2019年学术年会

作者：彭复蓉 Fu Yuhang Yu Yang Yang Yuheng Fang Anfei 毕朝位 College of plant protection Southwest University

Wheat stripe rust is one of the most important and devastating disease in wheat *** intense disease pressure,it is of great significance to quantify fungicide sensitivity of wheat stripe rust quickly and accurately for monitoring and controlling the disease.A rapid method based on detached leaf is proposed in this *** specific methods are as follows.5-10 μg/mL uredospore suspension,prepared by Tween 20(concentration 0.02%) was inoculated by art-brush(the diameter of0.03 mm) when wheat seedlings *** 8 d of inoculation,75 μg/mL 6-Benzylaminopurine and different concentrations of fungicide was added into 0.5% water *** prepared drugcontaining *** 9 cm circular filter papers were cut into 3 segments of 2.5 cm,4.0 cm,2.5 cm along the diameter,and the 4 cm filter paper was placed in the middle of the plate.5 cm middle leaf segments of infected wheat with obvious lesions were cut and placed on a 4 cm filter paper in parallel,andthe ends of the leaf segments were fixed with the remaining filter paper to ensure them adhered to the surface of the drug-containing *** dish contains 5 leaf segments of different *** 8 days of inoculation,the proportion of spore heap was accurately measured by *** control effect and50% effective concentration(EC) were calculated by *** test results show that this method can make the fungicide better acted on Pst through the isolated wheat leaf *** the sensitivity of Pst to triadimefon in this way is close to that of spraying potted wheat and the control effect is relatively *** of triadimefon to wheat stripe rust,collected in Chongqing,was determined by the above *** results showed the frequency distribution of 81 isolates to triadimefon distributed approximate normal(W=0.204).Therefore,the measured 0.1453 士 0.0081 μg/mL can be used as a baseline for the sensitivity of Pst to *** this method can be used to measure the vir

关键词： Puccinia striiformis f.sp.tritici Fungicide Sensitivity Detached leaves Triadimefon

Integrated metabolo-transcriptomics to reveal responses of wheat against Puccinia striiformis ***

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Integrated metabolo-transcriptomics to reveal responses of w...

中国植物病理学会2021年学术年会

作者： LIU Saifei XIE Liyang SU Jiaxuan TIAN Binnian FANG Anfei YU Yang BI Chaowei 杨宇衡 College of plant protection Southwest University

Wheat stripe rust is a widespread and harmful wheat disease caused by Puccinia striiformis ***（Pst） world *** targeted metabolome analyses and transcriptomics analyses of CYR23 infected leaves were performed to identify the differential metabolites and differentially expressed genes related to wheat disease *** observed up-regulation of 33 metabolites involved in the primary and secondary metabolism,especially for homogentisic acid,p-coumaroylagmatine and *** three metabolites were mainly involved in the phenylpropanoid metabolic pathway,hydroxycinnamic acid amides pathway,and saccharopine *** with transcriptome data on noncompatible interaction,the synthesis related genes of these three differential metabolites were all up-regulated *** gene regulatory network involved in response to Pst infection was constructed,and revealed that several transcription factor families including WRKYs,MYBs,and bZIPs were identified as potentially hubs in wheat resistance response against *** general,this research provides a novel and multi-faceted understanding for wheat disease resistance.

关键词： wheat stripe rust metabolomic transcriptomic gene regulatory network

Insertion Mutagenesis of Trichoderma atroviride by Restriction Enzyme-mediated DNA integration

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Journal of Shanghai Jiaotong university(Science) 2005年第S1期10卷 161-166,175页

作者：黄玉茜周晓英陈捷梁春浩范海延徐书法程根武刘海南 School of Agriculture and Biology Shanghai Jiaotong Univ. Shanghai 201101 ChinaDepart of Plant ProtectionShenyang Agricultural Univ. Shenyang 110161 School of Agriculture and Biology Shanghai Jiaotong Univ. Shanghai 201101 China institute of plant protection Liaoning Academy of Agricultural Sciences Shenyang 110161 Depart of plant protection Shenyang Agricultural Univ. Shenyang 110161

Restriction enzyme-mediated integration(REMI) of DNA has been recently received attention as a new technique for the generation of mutants by transformation in fungi. Trichoderma atroviride strain T23 was transformed with linearized plasmid pV2, conferring resistance to hygromycin B, in the presence of restriction enzyme used to linearize the plasmid. A total of 172 regeneration transformants were detected by successive inoculation for seven times subcultivation on fresh PDA plate containing hygromycin B. The plasmid was integrated stably into the chromosome DNA, which was confirmed by PCR and southern analysis. The difference between 172 transformants and the parent strain was confirmed in colonial color, sporulation and growth rate. The results showed that the significant difference appeared in above mentioned characters between transformants and parent strain is sporulation capability. Transformants TC6, TD5, TE7, TF1 and TK1 produced higher amounts of conidia than the parent strain T23. In addition, transformants TK1and TC6 showed stronger inhibition to the growth rate of the cucumber wilt pathogen (Fusarium oxyporum) in vitro.

关键词： Trichoderma atrovride restriction enzyme-mediated integration hygromycin resistance

Virulence comparison of Magnaporthe oryzae isolates between USA and Jilin of China and utilizing strategies of resistance to blast genes

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Virulence comparison of Magnaporthe oryzae isolates between ...

中国植物病理学会2015年学术年会

作者： WANG Ji-chun JIA Yu-lin LU Bao-hui WU Xian REN Jin-ping institute of plant protection Jilin Academy of Agricultural Sciences U.S.Department of Agriculture-Agricultural research Service Dale Bumpers National Rice Research Center

Rice blast is one of the most devastating crop diseases *** and planting blast-resistance cultivars are economic,effective and environment-friendly method to control rice *** master die isolates virulence and identify broad spectrum resistance gene types based on rice cultivars pathotypes interacted with representative strains are very important pathways for directing *** this report,44 isolates from Jilin Province and 14 race from USA of *** were inoculated onto 24 international rice monogenic lines（MLs） carrying a major blast resistance gene-Pia,Pib,Pii,Pik,Pik-h,Pik-m,Pik-p,Pik-s,Pish,Pit,Pita,Pita-2,Piz,Piz-t,Pil,Piz-5,Pi3,Pi5（t）,Pi7（t）,Pi9,Pi11（t）,Pi12（t）,PH9 and Pi20- and the susceptible reeurrent parent,Lijiangxintuanheigu（LTH）,under greenhouse *** percentage of pathotypes of the 44 isolates was found ranging from 8.3% to 79.2%,the frequency of resistant reactions of the MLs carrying Pi9,Pi19,Piz and Piz-5 were 94.2%,84.1%,81.8% and 81.8% in JiLin Province,*** 14 Magnaporthe oryzae isolates of USA were pathogenic to eight or more MLs,and the frequency of pathogenicity was ranged from 33.3%（race IG1） to 87.5%（race IE1）.The ML carrying Pi9 and Pita-2 demonstrated broad spectrum resistance to 14 representative USA races or isolates,and their resistance frequency was 92.9% and 78.6%,*** result provided import information for introducing and exchanging germplasms from ***,the pyramiding strategies with blast R genes for improving blast resistance in breeding lines were in progress and will be presented.

关键词： Magnaporthe oryzae rice blast monogenic line resistance gene breeding

Influence of different illuminations with xenon or microwave sulfur lamp on jointing and tillering of wheat

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Chinese Science Bulletin 2002年第17期47卷 1433-1437,1498页

作者： CHEN Qilin LIU Yuequn YU Xinjian CHEN Jinxing WENG Jun XU Chunhe Shanghai institute of plant Physiology Chinese Academy of SciencesShanghai 200032China institute of Electronic Lamp Fudan UniversityShanghai 200433China

The emission of microwave sulfur lamp is mainly composed of visible light. The sulfur lamp, producing little infrared radiation, has high efficiency, long duration of illumination and less energy consumption. In order to probe the agricultural application of the lamp, experiments were carried out with wheat (cv. Yangmai 158) to emphatically study illuminating effects of xenon or sulfur lamp on tillering, jointing and net photosynthetic rate during tillering and jointing periods. Results indicated that there is almost no difference in net photosynthetic rate of wheat leaves growing under different lamps. Xenon lamp significantly advanced the time of jointing, decreased number of tillers, number of total leaves per plant and leaf size, as well as inhibited root growth. In contrast with xenon lamp, sulfur lamp evidently delays the time of heading and grain maturation, increased number of heads per plant, head length, total number of grains per plant, head weight and total grain weight per plant,

关键词： sulfur lamp xenon lamp joint tiller wheat.

Anticaries and antimicrobial activities of methanolic extract from leaves of Cleistocalyx operculatus L.

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Asian Pacific Journal of Tropical Biomedicine 2017年第1期7卷 43-48页

作者： Phuong Thi Mai Nguyen Nadin Schultze Christin Boger Zeyad Alresley Albert Bolhuis Ulrike Lindequist Department of plant Biochemistry Institute of BiotechnologyVietnam Academy of Science and Technology institute of Pharmacy University of Greifswald Department of Pharmacy and Pharmacology University of Bath

Objective: To investigate antimicrobial activities of methanolic extract of leaves of Cleistocalyx operculatus L.(C. operculatus) grown in ***: The methanolic extract of C. operculatus leaves was phytochemically screened and tested for its antimicrobial activity against six Gram-positive bacteria(three of which were antibiotic multiresistant Staphylococcus spp.), two Gram-negative bacteria,and one fungal species using an agar diffusion method. Anticaries activity was tested using p H drop and biofilm assays formed in 96-well plastic ***: Phytochemical screening revealed the presence of flavonoids and terpenes, in which flavonoid content was 6.8 mg/g dry material. Antibacterial activity of the C. operculatus extract was shown only against Gram-positive bacteria Staphylococcus aureus, Bacillus subtilis and Streptococcus mutans GS-5(S. mutans), and three multiresistant bacteria being Staphylococcus epidermidis 847, Staphylococcus haemolyticus 535 and Staphylococcus aureus North German epidemic strain. Interestingly, methanolic extract of C. operculatus leaves exhibited the anticaries activity against S. mutans in terms of inhibition of acid production and biofilm formation. Activity of two key enzymes responsible for acidogenicity of S. mutans, F-ATPase and phosphotransferase system were inhibited by the extract with IC_(50) of 51.0 and 98.0 m g/m L, respectively. Cytotoxicity of the extract against keratinocytes was found only for higher concentrations [IC_(50)=(119.98 ± 4.63) m g/m L].Conclusions: The methanolic extract of C. operculatus leaves has the potential for development of antimicrobial preparations, especially anticaries products.

关键词： Cleistocalyx operculatus Antibacterial activity Anticaries Cytotoxicity

A transient expression system in soybean mesophyll protoplasts reveals the formation of cytoplasmic GmCRY1 photobody-like structures

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Science China(Life Sciences) 2019年第8期62卷 1070-1077页

作者： Lu Xiong Cong Li Hongyu Li Xiangguang Lyu Tao Zhao Jun Liu Zecheng Zuo Bin Liu institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing100081China Jilin Province Engineering Laboratory of plant Genetic Improvement College of Plant ScienceJilin UniversityChangchun130062China

Soybean(Glycine max(L.) Merr.), grown for its plant oils and proteins, is one of the most important crops throughout the *** stable and heritable transgenic soybeans is relatively inefficient;therefore, there is an urgent need for a simple and high-efficient transient transformation method by which to enable the investigation of gene functions in soybeans, which will facilitate the elucidation and improvement of the molecular mechanisms regulating the associated agronomic traits. We established a system of transient expression in soybean mesophyll protoplasts and obtained a high level of protoplast transfection efficiency(up to 83.5%). The subcellular activity of the protoplasts was well preserved, as demonstrated by the dynamic formation of GmCRY nucleus photobodies(NPs) and/or cytoplasmic photobody-like structures(CPs) in response to blue *** addition, we showed that GmCRY1b CPs colocalized with GmCOP1b, a co-ortholog of Arabidopsis thaliana CONSTITUTIVE PHOTOMORPHOGENIC 1(COP1), which provided new insight into the potential roles of GmCRY1s in the cytoplasm.

关键词： soybean protoplast transfection subcellular localization cryptochrome photobodies

Conventional Breeding and Molecular Markers for Blast Disease Resistance in Rice (Oryza sativa L.)

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Phyton-International Journal of Experimental Botany 2023年第3期92卷 725-746页

作者： Adel A.Rezk Mohamed M.El-Malky Hossam S.El-Beltagi Mohammed Al-daej Kotb AAttia Agricultural Biotechnology Department College of Agricultural and Food ScienceKing Faisal UniversityAl-Ahsa31982Saudi Arabia plant Pathology research institute Agriculture Research CenterGiza12619Egypt Rice research and Training Center SakhaField Crops Research InstituteAgricultural Research CenterKafr El-Sheikh33717Egypt Biochemistry Department Faculty of AgricultureCairo UniversityGiza12613Egypt Center of Excellence in Biotechnology research King Saud UniversityRiyadh11451Saudi Arabia Rice Biotechnology Laboratory Rice Research DepartmentField Crops Research InstituteARCKafr EL-Sheikh33717Egypt

Monogenic lines,which carried 23 genes for blast resistance were tested and used donors to transfer resistance genes by crossing *** results under blast nursery revealed that 9 genes from 23 genes were susceptible to highly susceptible under the three locations(Sakha,Gemmeza,and Zarzoura in Egypt);Pia,Pik,Pik-p,Piz-t,Pita,Pi b,Pi,Pi 19 and Pi ***,the genes Pii,Pik-s,Pik-h,Pi z,Piz-5,Pi sh,Pi 3,Pi 1,Pi 5,Pi 7,Pi 9,Pi 12,Pikm and Pita-2 were highly resistant at the same *** analysis confirmed the results,which divided into two groups;the first one included all the susceptible genes,while the second one included the resistance *** the greenhouse test,the reaction pattern of five races produced 100%resistance under artificial inoculation with eight genes showing complete resistance to all *** completely resistant genes:Pii,Pik-s,Piz,Piz-5(=bi2)(t),Pita(=Pi4)(t),Pita,Pi b and Pi1 as well as clustering analysis confirmed the *** the F1 crosses,the results showed all the 25 crosses were resistant for leaf blast disease under field ***,the results in F2 population showed seven crosses with segregation ratio of 15(R):1(S),two cross gave segregated ratio of 3 R:1 S and one gave 13:*** the identification of blast resistance genes in the parental lines,the marker K3959,linked to Pik-s gene and the variety IRBLKS-F5 carry this gene,which was from the monogenic *** results showed that four genotypes;Sakha 105,Sakha 103,Sakha 106 and IRBLKS-F5 were carrying Pik-s gene,while was absent in the Sakha 101,Sakha 104,IRBL5-M,IRBL9-W,IRBLTACP1 and IRBL9-W(R)*** for Pi 5 gene,the results showed that it was present in Sakha 103 and Sakha 104 varieties and absent in the rest of the *** addition,Pita-Pita-2 gene was found in the three Egyptian genotypes(Sakha 105,Sakha 101 and Sakha 104)plus IRBLTACP1 *** F2 generation,six populations were used to study the inheritance of blast resistance and s

关键词： Biotechnology tools clustering analysis monogenic lines resistance genes breeding Oryza sativa L.