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Phenotyping for Abiotic Stress Tolerance in Maize

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Journal of Integrative plant Biology 2012年第4期54卷 238-249页

作者： Benhilda Masuka Jose Luis Araus Biswanath Das Kai Sonder Jill E.Cairns International Maize and Wheat improvement Center(CIMMYT) unit of plant physiology Faculty of BiologyUniversity of Barcelona CIMMYT Km 45 Carretera Mexico-Veracruz

The ability to quickly develop germplasm having tolerance to several complex polygenic inherited abiotic and biotic stresses combined is critical to the resilience of cropping systems in the face of climate change. Molecular breeding offers the tools to accelerate cereal breeding; however, suitable phenotyping proto- cols are essential to ensure that the much-anticipated benefits of molecular breeding can be realized. To facilitate the full potential of molecular tools, greater emphasis needs to be given to reducing the within-experimental site variability, application of stress and characterization of the environment and appropriate phenotyping tools. Yield is a function of many processes throughout the plantcycle, and thus integrative traits that encompass crop performance over time or organization level （i.e. canopy level） will provide a better alternative to instantaneous measurements which provide only a snapshot of a given plant process. Many new phenotyping tools based on remote sensing are now available including non-destructive measurements of growth-related parameters based on spectral reflectance and infrared thermometry to estimate plant water status. Here we describe key field phenotyping protocols for maize with emphasis on tolerance to drought and low nitrogen.

关键词： Drought low nitrogen tolerance maize phenotyping spatial variability.

Dissecting Maize Productivity:Ideotypes Associated with Grain Yield under Drought Stress and Well-watered Conditions

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Journal of Integrative plant Biology 2012年第12期54卷 1007-1020页

作者： Jill E.Cairns Ciro Sanchez Mateo Vargas Raziel Ordoez Jose Luis Araus Global Maize Program International Maize and Wheat Improvement Center(CIMMYT)Mexico D.F.Mexico Bioinformatics and Statistics unit International Maize and Wheat Improvement Center(CIMMYT)Mexico D.F.Mexico unit of plant physiology Department of Plant BiologyUniversity of BarcelonaBarcelonaSpain

To increase maize （Zea mays L.） yields in drought-prone environments and offset predicted maize yield losses under future climates, the development of improved breeding pipelines using a multi-disciplinary approach is essential. Elucidating key growth processes will provide opportunities to improve drought breeding progress through the identification of key phenotypic traits, ideotypes, and donors. In this study, we tested a large set of tropical and subtropical maize inbreds and single cross hybrids under reproductive stage drought stress and well-watered conditions. Patterns of biomass production, senescence, and plant water status were measured throughout the crop cycle. Under drought stress, early biomass production prior to anthesis was important for inbred yield, while delayed senescence was important for hybrid yield. Under well-watered conditions, the ability to maintain a high biomass throughout the growing cycle was crucial for inbred yield, while a stay-green pattern was important for hybrid yield. While new quantitative phenotyping tools such as spectral reflectance （Normalized Difference Vegetation Index, NDVI） allowed for the characterization of growth and senescence patterns as well as yield, qualitative measurements of canopy senescence were also found to be associated with grain yield.

关键词： Climate change drought stress ideotypes maize senescence spectral reflectance.

The Relationship Between Developmental Accumulation of Leaf Soluble Proteins and Vernalization Response of Wheat(Triticum aestivum ***. Thell)

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Agricultural Sciences in China 2009年第4期8卷 410-417页

作者： M Majdi G Karimzadeh S Mahfoozi plant Breeding and Biotechnology Department Faculty of AgricultureTarbiat Modares UniversityP.O.Box 14115-336Tehran ***Iran physiology-Agronomy unit of Department of Cereals Research Seed and Plant Improvement InstituteKaraj 315854119Iran

The relationship between vernalization requirement and quantitative and qualitative changes in total leaf soluble proteins were determined in one spring （cv. Kohdasht） and two winter （cvs. Sardari and Norstar） cultivars of wheat （Triticum aestivum L.） exposed to 4℃. plants were sampled on days 2, 14, 21 and 35 of exposure to 4℃. The final leaf number （FLN） was determined throughout the vernalization periods （0, 7, 14, 24, and 35 d） at 4℃. The final leaf number decreased until days 24 and 35 in Sardari and Norstar eultivars, respectively, indicating the vernalization saturation at these times. No clear changes were detected in the final leaf number of Kohdash cultivar, verifying no vernalization requirement for this spring wheat cultivar. Comparing with control, clear cold-induced 2-fold increases in proteins quantity occurred after 48 h following the 4℃-treatment in the leaves of the both winter wheat cultivars but, such response was not detected in the spring cultivar. However, the electrophoretic protein patterns showed between-cultivar and between-temperature treatment differences. With increasing exposure time to 4℃, the winter cultivars tended to produce more HMW polypeptides than the spring cultivar. Similar proteins were induced in both Sardari and Norstar winter wheat cultivars, however, the long vernalization requirement in Norstar resulted in high level and longer duration of expression of cold-induced proteins compared to Sardari with a short vernalization requirement. These observations indicate that vernalization response regulates the expression of low temperature （LT） tolerance proteins and determines the duration of expression of LT- induced proteins.

关键词： soluble proteins wheat Triticum aestivum L. final leaf number vernalization cold

2,4-D Hyper Accumulation Induced Cellular Responses of Azolla pinnata R. Br. to Sustain Herbicidal Stress

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Phyton-International Journal of Experimental Botany 2020年第4期89卷 999-1017页

作者： Arnab Kumar De Arijit Ghosh Debabrata Dolui Indraneel Saha Malay Kumar Adak plant physiology and plant Molecular Biology Research unit Department of BotanyUniversity of KalyaniWest BengalIndia

In the present experiment with ongoing concentration(0µM,100µM,250µM,500µM and 1000µM)of 2,4-D,the responses of Azolla pinnata *** evaluated based on cellular ***,plants were significantly tolerated up to 1000µM of 2,4-D with its *** was accompanied by a steady decline of indole acetic acid(IAA)concentration in tissues with 78.8%over the *** bound H^(+)-ATPase activity was over expressed within a range of 1.14 to 1.25 folds with activator(KCl)and decreased within a range of 57.3 to 74.6%in response to inhibitor(Vanadate)*** regards to IAA metabolism,plants recorded a linear increase with wall bound oxidase activity up to maximum concentration of 2,*** variations were more moderated when wall bound IAA-oxidase recorded a linear increase proportionate to the 2,4-D *** was more extended with the presence of different isoforms of IAA-oxidase which was much more pronounced with distinct polymorphisms of expressed proteins,however,not independent to the 2,4-D *** like spermine,spermidine and putrescine(spm,spd and put)were not consistent in concentration with the dosages of 2,*** these,plants were induced to apoplastic NAD(P)H oxidase activity maximally by 1.6 folds under 500µM 2,4-D over ***,putrescine responded more or less consistently and recorded maximally 11.9 folds at 500µM 2,4-D as compared to the ***(P)H oxidase activity recorded maximally 1.6 folds against control and remain consistent throughout the concentrations of 2,*** along with APX were more linear in responses through the concentration of 2,4-D except CAT as compared to *** enzymatic antioxidative activity,peroxidases(GPX and APX)were overexpresed in a similar manner except for catalase with a non-significant *** stabilization of cellular redox,glutathione reductase attended maximum value by 2.45 folds at 1000µM evidenced with significant variations in protein polymo

关键词： Azolla 2,4-D antioxidative enzymes comet assay HPLC

Contribution of the ear and the flag leaf to grainfilling in durum wheat inferred from the carbon isotope signature: Genotypic and growing conditions effects

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Journal of Integrative plant Biology 2014年第5期56卷 444-454页

作者： Rut Sanchez-Bragado Abdelhalim Elazab Bangwei Zhou Maria Dolors Serret Jordi Bort Maria Teresa Nieto-Taladriz José Luis Araus unit of plant physiology Department of Plant Biology Faculty of Biology University of Barcelona National Institute for Agricultural and Food Research and Technology (INIA)

The ear, together with the flag leaf, is believed to play a major role as a source of assimilates during grain filling in C3cereals. However, the intrusive nature of most of the available methodologies prevents reaching conclusive results in this regard. This study compares the carbon isotope composition（d13C） in its natural abundance in the water‐soluble fractions of the flag leaf blade and the ear with the d13C of mature kernels to assess the relative contribution of both organs to grain filling in durum wheat（Triticum turgidum L. ***）. The relative contribution of the ear was higher in landraces compared to modern cultivars, as well as in response to nitrogen fertilization and water stress. Such genotypic and environmentally driven differences were associated with changes in harvest index（HI）, with the relative contribution of the ear being negatively associated with HI. In the case of the genotypic differences, the lower relative contribution of the ear in modern cultivars compared with landraces is probably associated with the appearance in the former of a certain amount of source limitation driven by a higher HI. In fact, the relative contribution of the ear was far more responsive to changes in HI in modern cultivars compared with landraces.

关键词： Carbon isotope discrimination ear flag leaf grain filling harvest index photosynthesis

Maize WI5 encodes an endo-1,4-β-xylanase required for secondary cell wall synthesis and water transport in xylem

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Journal of Integrative plant Biology 2020年第10期62卷 1607-1624页

作者： Xiaojiao Hu Yang Cui Xiaomin Lu Weibin Song Lei Lei Jinjie Zhu Jinsheng Lai Lizhu E Haiming Zhao State Key Laboratory of plant physiology and Biochemistry National Maize Improvement CenterCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China

Water transport from roots to leaves through xylem is important for plant growth and *** in water transport can cause drought stress,even when there is adequate water in the ***,we identi-fied the maize(Zea mays)wilty5(wi5)mutant,which ex-hibits marked dwarfing and leaf wilting throughout most of its life cycle under normal growth ***5 seed-lings exhibited lower xylem conductivity and wilted more rapidly under drought,NaCl,and high temperature treat-ments than wild-type ***-based cloning revealed that WI5 encodes an active endo-1,4-β-xylanase from gly-cosyl dehydration family 10,which mainly functions in degrading and reorganizing cell wall ***-transcription polymerase chain reaction andβ-glucuronidase assays revealed that WI5 is highly expressed in stems,especially in internodes undergoing secondary wall *** sequencing suggested that WI5 plays a unique role in internode *** and electron microscopy confirmed that wi5 is defective in xylan deposition and secondary cell wall *** deposition and xylan content were markedly re-duced in wi5 compared to the wild-type *** results suggest that WI5 functions in xylem cell wall thickening through its xylanase activity and thereby regulates xylem water transport,the drought stress response,and plant growth in maize.

关键词： dwarf fir transport

Genetic Identification of Quantitative Trait Loci for Contents of Mineral Nutrients in Rice Grain

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Journal of Integrative plant Biology 2009年第1期51卷 84-92页

作者： Ana Luisa Garcia-Oliveira Department of plant Genetics and Breeding and State Key Laboratory of plant physiology and Biochemistry China Agricultural University National Centre for Evaluation of Agricultural Wild plant Rice Beijing Key Laboratory of Crop Genetic improvement and Genome of Ministry of Agriculture

In present study, Fe, Zn, Mn, Cu, Ca, Mg, P and K contents of 85 introgression lines （ILs） derived from a cross between an elite indica cultivar Teqing and the wild rice （Oryza rufipogon） were measured by inductively coupled argon plasma （ICAP） spectrometry. Substantial variation was observed for all traits and most of the mineral elements were significantly positive correlated or independent except for Fe with Cu. A total of 31 putative quantitative trait loci （QTLs） were detected for these eight mineral elements by single point analysis. Wild rice （O. rufipogon） contributed favorable alleles for most of the QTLs （26 QTLs）, and chromosomes 1,9 and 12 exhibited 14 QTLs （45%） for these traits. One major effect of QTL for zinc content accounted for the largest proportion of phenotypic variation （11%-19%） was detected near the simple sequence repeats marker RM152 on chromosome 8. The co-locations of QTLs for some mineral elements observed in this mapping population suggested the relationship was at a molecular level among these traits and could be helpful for simultaneous improvement of these traits in rice grain by marker assisted selection.

关键词： introgression lines mineral elements Oryza sativa Oryza rufipogon quantitative trait loci.

Genetic architecture of embryo size and related traits in maize

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The Crop Journal 2022年第1期10卷 204-215页

作者： Xiaowei Li Min Wang Renyu Zhang Hui Fang Xiuyi Fu Xiaohong Yang Jiansheng Li State Key Laboratory of plant physiology and Biochemistry National Maize Improvement Center of ChinaMOA Key Laboratory of Maize BiologyBeijing Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China

The embryo in maize has a critical role in controlling kernel nutrition components and grain *** measured five embryo weight and size traits,six kernel weight and size traits,and five embryo-tokernel ratio traits in a nested association mapping(NAM)population of 611 recombinant inbred lines(RILs)derived from four inbred lines including the high-oil,giant-embryo line BY815 as the common *** three statistical methods,we identified 5–22 quantitative trait loci(QTL)for each trait,explaining 4.7%–46.7%of the phenotypic *** genetic architecture of maize embryo size and its related traits appeared to be dominated by multiple small-effect loci with little epistasis,and the genetic control underlying embryo size appeared to be distinct from that underlying kernel size.A trait–QTL association network included 205 nodes and 439 edges and revealed 28 key loci associated with at least three *** maize genes including Zm Urb2,Emp12 and Zm BAM1 d,maize orthologs of known rice genes that control seed size including BG1,XIAO and GS9,and 11 maize orthologs of Arabidopsis EMBRYO-DEFECTIVE(EMB)genes were identified as underlying these key ***,the phenotypic and genetic relationships between embryo size and kernel size were evaluated,and genetic patterns for identified loci that control embryo size and its related traits were *** findings reveal distinct genetic architectures for embryo size,kernel size,and embryo-to-kernel ratio traits and establish a foundation for the improvement of embryo-size-mediated kernel nutrition and grain yield.

关键词： Embryo size Kernel size Quantitative trait loci Genetic relationship

Combining QTL mapping and expression profile analysis to identify candidate genes of cold tolerance from Dongxiang common wild rice(Oryza rufipogon Griff.)

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Journal of Integrative Agriculture 2016年第9期15卷 1933-1943页

作者： ZHAO Jie QIN Jing-jing SONG Qian SUN Chuan-qing LIU Feng-xia State Key Laboratory of plant physiology and Biochemistry National Center for Evaluation of Agricultural Wild Plants (Rice)/Beijing Key Laboratory of Crop Genetic Improvement/Department of Plant Genetics and Breeding China Agricultural University

Rice（Oryza sativa L.）, a tropical and subtropical crop, is susceptible to low temperature stress during seedling, booting, and flowering stages, which leads to lower grain quality levels and decreasing rice yields. Cold tolerance is affected by multiple genetic factors in rice, and the complex genetic mechanisms associated with chilling stress tolerance remain unclear. Here, we detected seven quantitative trait loci（QTLs） for cold tolerance at booting stage and identified one cold tolerant line, SIL157, in an introgression line population derived from a cross between the indica variety Guichao 2, as the recipient, and Dongxiang common wild rice, as the donor. When compared with Guichao 2, SIL157 showed a stronger cold tolerance during different growth stages. Through an integrated strategy that combined QTL-mapping with expression profile analysis, six candidate genes, which were up-regulated under chilling stress at the seedling and booting developmental stages, were studied. The results may help in understanding cold tolerance mechanisms and in using beneficial alleles from wild rice to improve the cold tolerance of rice cultivars through molecular marker-assisted selection.

关键词： common wild rice introgression line low temperature tolerance different growth stages candidate genes