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Agricultural Science & technology 2016年第6期17卷 1326-1330页

作者： Yanxiang QI He ZHANG Yixian XIE Xin ZHANG Ying LU Qunfang YU Huiqiang ZHANG Jinji PU Environment and plant Protection Institute Chinese Academy of Tropical Agricultural Sciences

[Objective] This study aimed to develop a PCR assay for detecting Xanthomonas campestris pv. mangiferaeindicae(Xcm) in culture and in planta. [Method] Primers(Xcm HF and Xcm HR) were designed based on the partial sequence of hrp B gene from xanthomonads to develop a PCR assay for Xcm. Furthermore, specificity and sensitivity of the primer pairs were analyzed in detection of genomic DNA and cell from Xcm. [Result] Amplication was positive only with genomic DNA from positive control ATCC11637 and 12 Xcm strains; no PCR products were amplified with genomic DNA from ten other xanthomonads and seven other bacterial species. The sensitivity of detection was 2.4 pg/μl genomic DNA, and 1.8 × 104CFU/ml cells. The primers also worked well for pathogen detection in direct PCR assays of Xcm colonies grown on liquid medium and in PCR assays of total DNA from leaf, branch and fruit lesions. [Conclusion] A PCR assay was successfully established for rapid detection of Xcm in culture and in planta.

关键词： genomic campestris Xanthomonas primer DNA branch detecting aimed sterile amplification

Resveratrol Supplementation Improves the Development of Interspecies Nuclear Transfer Embryos Reconstructed with Sika Deer Somatic cells and Bovine Ooplasm

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Resveratrol Supplementation Improves the Development of Inte...

Identification of Phaeoacremonium aleophillum Phytotoxin Decline Platanus Trees Platanus occidentalies L.

2015中国遗传学会大会

作者： Shiyong Wang Junjun Zheng Yuechun Yang Zongyue Liu Fuhe Yang State Key Laboratory for Molecular Biology of Special Economical Animals Institute of Special Economic Animal and Plant SciencesCAAS College of Animals Science Jilin Academy of Agricultural Science and Technology

The development of somatic cell nuclear transfer(SCNT)embryos remains *** efficiency of SCNT is dependent on production of viable *** in vitro culture environment exerts significant effects on the embryos *** has shown that SCNT embryos have low development because of reactive oxygen accumulation in SCNT *** we all know the antioxidant can improve the embryo’s developmental ***,a natural antioxidant found in plants,can inhibit the generation of free radical,oxidation of lipid and regulate the enzyme activity related to *** also can regulate the development of ovaries and *** the maturation process of bovine oocyte in vitro,resveratrol can enhance the protein expression of SIRT1 *** can improve the quality of oocyte by enhancing the quality and quantity of mitochondria in oocyte *** this study,to investigate the resveratrol whether can improve the development of sika deer-bovine interspecies somatic cell nuclear transfer(ISCNT)embryos in vitro,different concentrations of resveratrol were supplemented in the culture *** sika deer ear skin fibroblasts were used as donor cell and bovine ooplasm were used as recipient to produce the ISCNT *** deer-bovine reconstructed embryos were randomly divided into 4 groups(each group was repeated 5 times)which cultured in CRlaa with different concentration(0,0.25,0.5 and 1μMol·L)of *** of cleavage and blastocyst development were recorded after 48 h and 7 days *** culture medium was changed in half volume every 48 h *** results showed that different concentrations of resveratrol had no significant effect on the cleavage rate of the reconstructed embryos(P>0.05).The blastocyst rate were 38%and 36.7%in groups which cultured with 0.50 and1μMol·Lresveratrol,significantly higher than 0 and 0.25 uMol-L"1 group(8%and 10%,P0.05).In summary,adding

关键词： Interspecies Nuclear Transfer Sika Deer Bovine Resveratrol

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Journal of Agricultural Science and technology(A) 2012年第8期2卷 983-987页

作者： Khalid Hassan Taha Department of plant Protection College of Agriculture and Forestry University of Mosul Iraq

The genus Phaeoacremonium is associated die-back of various woody hosts. In this study, Phaeoaeremonium specie was isolated from necrotic woody tissue of Platanus occidentalies L. （Platanus）. Morphological and cultural characteristics were used to identify the species, which known, and being described as Phaeoacremonium aleophillum. This is the first report on identifying Phaeoacremonium aleophillum from Platanus in Iraq, previously reports accommodate Cephalosporium acremonium from Platanus trees. This specie is able to produce toxic metabolites. Applications of P. aleophillum crude and cell-free filtrates induced severe chlorosis and necrosis on Platanus leaves after 24 hours of treatment with P. aleophillum culture filtrate. A novel phytotoxin was isolated from P. aleophillurn and culture filtrate was identified as galactouronic acid.

关键词： Phaeoacremonium aleophillum Platanus phytotoxin galactouronic acid.

Inhibitory effect of Salicornia europaea on the marine alga Skeletonema costatum

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Science China(Life Sciences) 2012年第6期55卷 551-558页

作者： JIANG Dan HUANG LingFeng LIN YongQing NIE LingLing LV SuLian KUANG TingYun LI YinXin Key Laboratoty of plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China Graduate University of Chinese Academy of Sciences Beijing 100080China College of Oceanography and Environmental Science Xiamen UniversityXiamen 361005China

Exploiting the negative biochemical interference between plants and algal species has been suggested as a method to control harmful algal blooms. In this work, we investigated the inhibitory effect of the salt marsh halophyte Salicornia europaea against the marine alga Skeletonema costatum. S. europaea suppressed the growth of S. costatum in a nutrient-sufficient co-culture system, indicating that the inhibition of algal growth was because of the phytotoxic effect of S. europaea, rather than nutrient competition. We tested aqueous and organic extracts from S. europaea roots against S. costatum. The organic ex- tracts inhibited growth and affected the cell size and chlorophyll a content of S. costatum in a dose-dependent manner. Among the three tested organic extracts, the methanol extract had the greatest effects on S. costatum, followed by butanol extract, and then the chloroform extract. Two flavonoids, rutin and quercetin-3-13-D-glucoside, were identified in the methanol extract by high performance liquid chromatography. The concentration of rutin was much higher than that of quercetin-3-13-D-glucoside. In an algal bioassay, rutin inhibited the growth of S. costatum and the inhibitory effect increased with increasing rutin concen- tration and with decreasing initial algal density. Therefore, we concluded that S. europaea negatively affects the growth of S. costatum, and that rutin, a metabolite of S. europaea, may play a role in this inhibitory effect.

关键词： dose-dependent root extracts rutin Salicornia europaea Skeletonema costatum

Profile of the culturable microbiome capable of producing acyl-homoserine lactone in the tobacco phyllosphere

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Journal of Environmental Sciences 2013年第2期25卷 357-366页

作者： Di Lv Anzhou Ma Xuanming Tang Zhihui Bai Hongyan Qi Guoqiang Zhuang Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Institute of Agro-products Processing Science and technology Chinese Academy of Agricultural Sciences

Bacterial populations coexisting in the phyllosphere niche have important effects on plant health. Quorum sensing （QS） allows bacteria to communicate via diffusible signal molecules, but QS-dependent behaviors in phyllosphere bacterial populations are poorly understood. We investigate the dense and diverse N-acyl-homoserine lactone （AHL）-producing phyllosphere bacteria living on tobacco leaf surfaces via a culture-dependent method and 16S rRNA gene sequencing. Our results indicated that approximately 7.9%-11.7% of the culturable leaf-associated bacteria have the ability to produce AHL based on the assays using whole-cell biosensors. Sequencing of the 16S rRNA gene assigned the AHL-producing strains to two phylogenetic groups, with Gammaproteobacteria （93%） as the predominant group, followed by Alphaproteobacteria. All of the AHL-producing Alphaproteobacteria were affiliated with the genus Rhizobium, whereas the AHL-producing bacteria belonging to the Gammaproteobacteria mainly fell within the genera Pseudomonas, Acinetobacter, Citrobacter, Enterobacter, Pantoea and Serratia. The bioassays of supernatant extracts revealed that a portion of the strains have a remarkable AHL profile for AHL induction activity using the two different biosensors, and one compound in the active extract of a representative isolate, NTL223, corresponded to 3-oxo-hexanoyl-homoserine lactone. A large population size and diversity of bacteria capable of AHL-driven QS were found to cohabit on leaves, implying that cross-communication based AHL-type QS may be common in the phyllosphere. Furthermore, this study provides a general snapshot of a potential valuable application of AHL-producing bacteria inhabiting leaves for their presumable ecological roles in the phyllosphere.

关键词： phyllosphere bacteria N-acyl-homoserine lactone interaction

Directional changes of species spatial dispersion and realized environmental niches drive plant community assembly during early plant succession

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Journal of plant Ecology 2019年第3期12卷 409-418页

作者： Werner Ulrich Markus Klemens Zaplata Susanne Winter Anton Fischer Department of Ecology and Biogeography Nicolaus Copernicus University in TorunLwowska 1PL 87-100 ToruńPoland Hydrology and Water Resources Management Brandenburg University of Technology Cottbus-SenftenbergSiemens-Halske-Ring 10D-03046 CottbusGermany Faculty of Forest and Environment Section of Applied Ecology and ZoologyEberswalde University of Sustainable DevelopmentAlfred-Möller-Str.5D-16225 EberswaldeGermany Department of Geobotany Center of Life and Food SciencesTechnische Universität MünchenHans-Carl-von-Carlowitz-Platz 2D-85354 FreisingGermany

Aims Probabilistic models of species co-occurrences predict aggre-gated intraspecific spatial distributions that might decrease the degree of joint species occurrences and increase community ***,little is known about the influence of intraspecific aggre-gation on the co-occurrence of species in natural,species-rich ***,we focus on early plant succession and ask how changes in intraspecific aggregation of colonizing plant spe-cies influence the pattern of species co-existence,richness and *** We studied the early vegetation succession in a six ha constructed catchment within the abandoned part of a lignite mine in NE *** two spatial scales(1-and 25-m2 plots),we compared for each pair of species the intraspecific degree of aggregation and the pattern of co-occurrence and compared observed rela-tionships with temporal changes in important species functional *** Findings The majority of species occurred in an aggregated manner,particu-larly in the first 2 years of *** pairwise comparisons,we found an excess of segregated species occurrences leading to a posi-tive link between intraspecific aggregation and pairwise species seg-regation as predicted by the aggregation hypothesis,particularly at the lower spatial *** degree of intraspecific aggregation was negatively correlated with the community-wide level of species spatial turnover and with plot species *** results are the first direct confirmation that increasing intraspecific aggregation and interspecific competitive interactions counteract in shaping plant community structure during *** respective effects of aggregation were strongest at intermediate states of early succession.

关键词： primary succession spatial analysis environmental niche functional traits species aggregation

Potential of four marine-derived fungi extracts as anti-proliferative and cell death-inducing agents in seven human cancer cell lines

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Asian Pacific Journal of Tropical Medicine 2015年第10期8卷 782-790页

作者： Alice Abreu Ramos Maria Prata-Sena Bruno Castro-Carvalho Tida Dethoup Suradet Buttachon Anake Kijjoa Eduardo Rocha Interdisciplinary Center for Marine and Environmental Research(CIIMAR) CIMAR Associate Laboratory(CIMAR LA)University of Porto(U.Porto) ICBAS-Institute of Biomedical Sciences Abel Salazar University of Porto(U.Porto) Department of plant Pathology Faculty of AgricultureKasetsart University

Objective:To evaluate the in vitro anticancer activity of crude ethyl acetate extracts of the culture of four marine-derived fungi Aspergillus similanensis KUFA 0013(E1),Neosartorya paulistemis KUFC 7897(E2),Neosartorya siamensis KUFA 0017(E4) and Talaromyces trachyspermus KUFC 0021(E3) on a panel of seven human cancer cell ***:Effects on cell proliferation,induction of DNA damage and cell death were assessed by MTT and clonogenic assays,comet assay and nuclear condensation assay,***:The proliferation of HepG2,HCTl 16 and A375 cells decreased after incubation with the extracts E2 and *** anti-proliterative effect was confirmed by morphologic alterations and by clonogenic *** extracts also induced cell death in HepG2 and HCT116 *** was used as a positive control and showed in vitro anticancer ***:This study demonstrated,for the first time,that extracts of Neosartorya paulistensis and Neosartorya siamensis have selective anti-proliferative and cell death activities in HepG2,HCT16 and A375 *** bioactivity of these extracts suggests a potential for biotechnological applications and substantiates that both should be further considered for the elucidation of the molecular targets and signal transduction pathways involved.

关键词： Anti-proliferative activity Antineoplastic agents

Sterilization of <i>Hibiscus rosa-sinensis</i>L. Vegetative Explants Sourced from plants Grown in Open Environment and Influences of Organic Ingredients on <i>In Vitro</i>Direct Regeneration

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American Journal of plant Sciences 2012年第6期3卷 791-798页

作者： Chew Tiong Dar Janna Ong Abdullah Parameswari Namasivayam Siti Habsah Roowi Department of cell and Molecular Biology Faculty of Biotechnology and Biomolecular Sciences Universiti Putra Malaysia Ser-dang Malaysia Department of Microbiology Faculty of Biotechnology and Biomolecular Sciences Universiti Putra Malaysia Serdang Malaysia PT Lengkuk technology Felda Biotechnology Centre Nilai Malaysia.

This paper reports on the effects of organic ingredients in facilitating direct shoot regeneration from nodal explants of Hibiscus rosa-sinensis L. This paper also compares the sterilization conditions for 3 types of explants (node, internode, and shoot tip) harvested from an open field. The optimized sterilization conditions for the explants were 40% Clorox- 20 min exposure, 10% Clorox-15 min exposure, and 5% Clorox-40 min exposure for the node, internode and shoot tip, respectively. In the direct shoot regeneration using the nodal explants, we found MS medium containing 40 g/L sucrose, 0.3% (w/v) activated charcoal, and supplementations with myo-inositol, thiamine and nicotinic acid were suitable. The in vitro shoot survival rate was 30% with a mean leaf numbers of 2.68 produced, and a mean leaf length of 1.71 cm achieved after 5 weeks of culture on the modified medium.

关键词： Hibiscus rosa-sinensis L. Murashige and Skoog Medium Axillary Bud Sucrose Organic Ingredient Activated Charcoal

Developmental characteristics and response to iron toxicity of root border cells in rice seedlings

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第3期9卷 261-264页

作者： Cheng-hua XING Mei-hong ZHU Miao-zhen CAI Peng LIU Gen-di XU Shao-hui WU Bioengineering Institute Jinhua College of Profession and Technology Jinhua 321007 China College of Chemistry and Life Sciences Zhejiang Normal University Jinhua 321004 China

To investigate the Fe^2＋ effects on root tips in rice plant, experiments were carried out using border cells in vitro. The border cells were pre-planted in aeroponic culture and detached from root tips. Most border cells have a long elliptical shape. The number and the viability of border cells in situ reached the maxima of 1600 and 97.5%, respectively, at 20---25 mm root length. This mortality was more pronounced at the first 1-12 h exposure to 250 mg/L Fe^2＋ than at the last 12-36 h. After 36 h, the cell viability exposed to 250 mg/L Fe^2＋ decreased to nought, whereas it was 46.5% at 0 mg/L Fe^2＋. Increased Fe^2＋ dosage stimulated the death of detached border cells from rice cultivars. After 4 h Fe^2＋ treatment, the cell viabilities were _〉80% at 0 and 50 mg/L Fe^2＋ treatment and were 〈62% at 150, 250 and 350 mg/L Fe^2＋ treatment; The viability of border cells decreased by 10% when the Fe^2＋ concentration increased by 100 mg/L. After 24 h Fe^2＋ treatment, the viabilities of border cells at all the Fe^2＋ levels were 〈65%; The viability of border cells decreased by 20% when the Fee＋ concentration increased by 100 mg/L. The decreased viabilities of border cells indicated that Fe^2＋ dosage and treatment time would cause deadly effect on the border cells. The increased cell death could protect the root tips from toxic harm. Therefore, it may protect root from the damage caused by harmful iron toxicity.

关键词： Rice plant Border cells Iron toxicity

Advancements in plant regeneration and genetic transformation of grapevine(Vitis spp.)

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Journal of Integrative Agriculture 2021年第6期20卷 1407-1434页

作者： ZHANG Xiu-ming WU Yi-fei LI Zhi SONG Chang-bing WANG Xi-ping Key Laboratory of Horticultural plant Biology and Germplasm Innovation in Northwest China Ministry of Agriculture/State Key Laboratory of Crop Stress Biology in Arid Areas/College of HorticultureNorthwest A&F UniversityYang ling 712100P.R.China College of Biological Science and Engineering North Minzu UniversityYinchuan 750021P.R.China

Grapevine(Vitis spp.)is one of the most economically important fruit crops worldwide,and there is considerable interest in improving its major agronomic and enological traits in response to ever-changing agricultural environments and consumer *** genetic techniques in particular,associated with rapid technological advancements,provide an attractive alternative to conventional breeding approaches for developing new grapevine varieties with enhanced yield performance,quality,stress tolerance and disease *** date,several grapevine varieties have been transformed with genes associated with diverse functions through biolistic bombardment and/or Agrobacterium-mediated transformation,and transgenic grape lines have been obtained using established regeneration ***,a wide range of factors,including genotype,explant source and culture medium,have been shown to affect the efficiency of plant ***,the selection and use of acceptor materials,bacterial strain and cell density,selectable markers and selection methods also influence transformation *** paper provides an overview of recent advances in grapevine regeneration and genetic transformation and in-depth discussion of the major limiting factors,and discusses promising future strategies to develop robust plant regeneration and genetic transformation in grapevine.

关键词： grapevine organogenesis somatic embryogenesis plant regeneration genetic transformation