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Leaf Morphology Genes SRL1 and RENL1 Co-Regulate cellulose Synthesis and Affect Rice Drought Tolerance

Rice science 2024年第1期31卷 103-117,I0020-I0022页

作者： LIU Dan ZHAO Huibo WANG Zi’an XU Jing LIU Yiting WANG Jiajia CHEN Minmin LIU Xiong ZHANG Zhihai CEN Jiangsu ZHU Li HU Jiang REN Deyong GAO Zhenyu DONG Guojun ZHANG Qiang SHEN Lan LI Qing QIAN Qian HU Songping ZHANG Guangheng State Key Laboratory of Rice Biology and Breeding China National Rice Research InstituteHangzhou 310006China Research Center of plant Functional Genes and Tissue culture technology College of Bioscience and BioengineeringJiangxi Agricultural UniversityNanchang 330045China Qian Qian Academician Workstation National Nanfan Research Institute(Sanya)Chinese Academy of Agricultural SciencesSanya 572024China

The morphological development of rice(Oryza sativa L.)leaves is closely related to plant architecture,physiological activities,and ***,it is unclear whether there is a co-regulatory relationship between the morphological development of leaves and adaptation to drought *** this study,a drought-sensitive,roll-enhanced,and narrow-leaf mutant(renl1)was induced from a semi-rolled leaf mutant(srl1)by ethyl methane sulfonate(EMS),which was obtained from Nipponbare(NPB)through ***-based cloning and functional validation showed that RENL1 encodes a cellulose synthase,allelic to NRL1/*** RENL1 mutation resulted in reduced vascular bundles,vesicular cells,cellulose,and hemicellulose contents in cell walls,diminishing the water-holding capacity of *** addition,the root system of the renl1 mutant was poorly developed and its ability to scavenge reactive oxygen species(ROS)was decreased,leading to an increase in ROS after drought ***,genetic results showed that RENL1 and SRL1 synergistically regulated cell wall *** results revealed a theoretical basis for further elucidating the molecular regulation mechanism of cellulose on rice drought tolerance,and provided a new genetic resource for enhancing the synergistic regulation network of plant type and stress resistance,thereby realizing simultaneous improvement of multiple traits in rice.

关键词： cellulose cell wall drought tolerance leaf morphology rice

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Macroalgal deep genomics illuminate multiple paths to aquatic,photosynthetic multicellularity

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Molecular plant 2024年第5期17卷 747-771页

作者： David R.Nelson Alexandra Mystikou Ashish Jaiswal Cecilia Rad-Menendez Michael J.Preston Frederik De Boever Diana C.El Assal Sarah Daakour Michael W.Lomas Jean-Claude Twizere David H.Green William C.Ratcliff Kourosh Salehi-Ashtiani Division of Science and Math New York University Abu DhabiAbu DhabiUAE Center for Genomics and Systems Biology(CGSB) New York University Abu DhabiAbu DhabiUAE Biotechnology Research Center Technology Innovation InstitutePO Box 9639Masdar CityAbu DhabiUAE culture Collection of Algae and Protozoa Scottish Association for Marine ScienceObanScotlandUK National Center for Marine Algae and Microbiota Bigelow Laboratory for Ocean SciencesEast BoothbayMEUSA Laboratory of Viral Interactomes GIGA InstituteUniversity of LiegeLiegeBelgium School of Biological Sciences Georgia Institute of TechnologyAtlantaGAUSA

Macroalgae are multicellular,aquatic autotrophs that play vital roles in global climate maintenance and have diverse applications in biotechnology and eco-engineering,which are directly linked to their multicellularity ***,their genomic diversity and the evolutionary mechanisms underlying multicellularity in these organisms remain *** this study,we sequenced 110 macroalgal genomes from diverse climates and phyla,and identified key genomic features that distinguish them from their microalgal *** for cell adhesion,extracellular matrix formation,cell polarity,transport,and cell differentiation distinguish macroalgae from microalgae across all three major phyla,constituting conserved and unique gene sets supporting multicellular *** genes show phylum-and climate-specific expansions that may facilitate niche ***,our study reveals genetic determinants of convergent and divergent evolutionary trajectories that have shaped morphological diversity in macroalgae and provides genome-wide frameworks to understand photosynthetic multicellular evolution in aquatic environments.

关键词： macroalgae multicellularity comparative genomics evolution adhesome endogenous viral elements

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Gamma Irradiation of Embryogenic Callus cultures and In vitro Selection for Salt Tolerance in Sugarcane (Saccharum officinarum L.)

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Agricultural Sciences in China 2008年第9期7卷 1147-1152页

作者： Vikas Y Patade P Suprasanna V A Bapat plant cell culture technology section Nuclear Agriculture & Biotechnology DivisionBhabha Atomic Research Centre

Radiation induced mutagenesis followed by in vitro selection was employed for salt tolerance in popular Indian sugarcane （Saccharum officinarum L.） cv. CoC-671. Embryogenic calli were gamma irradiated and exposed to different levels of NaCl （42.8, 85.6, 128.3, 171.1,213.9, 256.7, 299.5, or 342.2 mM）. The relative growth rate （RGR） decreased progressively with increasing salt stress and was the least with a salt stress of 256.7 mM （0.25±0.009）, almost 10 fold lesser than the control. The RGR was significantly lower in 85.6 mM and higher salt stressed calli than the control. The survival percent also decreased, with an increase in NaCl concentration. In case of 10 and 20 Gy irradiated calli, regeneration was observed up to 85.6 mM NaCl selection, medium, whereas, higher treatments （128.3 mM and beyond） exhibited browning initially. However, in the subsequent subcultures, regeneration was obtained in the case of 10 and 20 Gy irradiated calli on 128.3 and 171.1 mM NaCl selections. Higher dose of gamma irradiation （40 Gy） also showed regeneration, but only with 85.6 mM NaCI selection. The unirradiated calli regenerated the highest number of plantlets followed by 10 and 20 Gy irradiated calli on salt selection. A total of 147 plantlets were selected from different salt levels. The salt selected plants are being tested for their field performance.

关键词： sugarcane in vitro mutagenesis in vitro selection salt tolerance

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A critical review on natural compounds interacting with the plant plasma membrane H^(+)-ATPase and their potential as biologicals in agriculture

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Journal of Integrative plant Biology 2022年第2期64卷 268-286页

作者： Nanna W.Havshφi Anja T.Fuglsang Department of plant and Environmental Sciences Section for Transport BiologyUniversity of CopenhagenFrederiksbergCopenhagenDK-1871 Denmark

The plant plasma membrane(PM)H^(+)-ATPase is an essential enzyme controlling plant growth and *** is an important factor in response to abiotic and biotic stresses and is subject to tight *** are in demand for new sustainable natural growth regulators and as a key enzyme for regulation of transport into the plant cell the PM H^(+)-ATPase is a potential target for *** this review,we have evaluated the known non-protein natural compounds with regulatory effects on the PM H^(+)-ATPase,focusing on their mechanism of action and their potential as biologicals/growth regulators in plant production of future sustainable agriculture.

关键词： biologicals cyclic-lipodepsipeptides H^(+)-ATPase H+pump natural compounds phosphatidylinositol plasma membrane pore forming compound tenuazonic acid

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Direct somatic embryogenesis and related gene expression networks in leaf explants of Hippeastrum ‘Bangkok Rose’

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Horticultural plant Journal 2024年第2期10卷 556-572页

作者： Jingjue Zeng Yi Deng Shahid Iqbal Jiarui Zhang Kunlin Wu Guohua Ma Lin Li Guangyi Dai Rufang Deng Lin Fang Songjun Zeng Guangdong Provincial Key Laboratory of Applied Botany South China Botanical GardenChinese Academy of SciencesGuangzhouGuangdong 510650China University of the Chinese Academy of Sciences Beijing 100049China South China National Botanical Garden GuangzhouGuangdong 510650China Zhaoqing Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and technology ZhaoqingGuangdong 526000China Horticultural Science Department North Florida Research and Education CenterUniversity of Florida/IFASQuincyFL 32351USA Public Laboratory of South China Botanical Garden Chinese Academy of SciencesGuangzhouGuangdong 510650China Key Laboratory of South China Agricultural plant Molecular Analysis and Gene Improvement South China Botanical GardenChinese Academy of SciencesGuangzhouGuangdong 510650China

Hippeastrum, a highly diverse genus in the Amaryllidaceae family, is a valuable ornamental bulbous flowering plant. Somatic embryogenesis(SE) is an efficient method for mass production of Hippeastrum plantlets. Previous studies have been devoted to the in vitro propagation of Hippeastrum, but the SE and its regulatory networks are rarely reported. In this study, we established a direct SE method of Hippeastrum Bangkok Rose' using leaf bases as explants. MS supplemented with 1.00 mg·L^(-1)NAA +1.00 mg·L^(-1)KT + 0.25 mg·L^(-1)TDZ was the optimal medium for SE. Histological observations showed that the bipolar somatic embryo originated from the epidermal cell layer and underwent initiation,globular, scutellar and coleoptile stages. During SE, endogenous hormones of IAA, CTK, ABA, and SA were highly accumulated. Transcriptomic analysis revealed the genes encoding auxin biosynthesis/metabolic enzymes and efflux carriers were induced, while the auxin receptor of TIR1 and ARF transcriptional repressor of Aux/IAA were down-regulated and up-regulated, respectively, leading to suppression of auxin signaling. In contrast, cytokine signaling was promoted at the early stage of SE, as biosynthesis, transport, and signaling components were *** stress-related genes were up-regulated at the early or late stages of SE. Chromatin remodeling could also be dynamically regulated via distinct expression enzymes that control histone methylation and acetylation during SE. Moreover, key SE regulators, including WOXs and SERKs were highly expressed along with SE. Overall, the present study provides insights into the SE regulatory mechanisms of the Hippeastrum.

关键词： Hippeastrum Tissue culture Somatic embryogenesis Gene regulation

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Improvements of TKC technology Accelerate Isolation of Transgene-Free CRISPR/Cas9-Edited Rice plants

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Rice science 2019年第2期26卷 109-117,I0016,I0017页

作者： HE Yubing ZHU Min WANG Lihao WU Junhua WANG Qiaoyan WANG Rongchen ZHAO Yunde National Key Laboratory of Crop Genetic Improvement and National Center of plant Gene Research (Wuhan) Huazhong Agricultural University College of Life Science and technology Huazhong Agricultural University College of plant Science & technology Huazhong Agricultural University section of cell and Developmental Biology University of California San Diego

Elimination of the CRISPR/Cas9 constructs in edited plants is a prerequisite for assessing genetic stability, conducting phenotypic characterization, and applying for commercialization of the plants. However, removal of the CRISPR/Cas9 transgenes by genetic segregation and by backcross is laborious and time consuming. We previously reported the development of the transgene killer CRISPR(TKC) technology that uses a pair of suicide genes to trigger self-elimination of the transgenes without compromising gene editing efficiency. The TKC technology enables isolation of transgene-free CRISPR-edited plants within a single generation, greatly accelerating crop improvements. Here, we presented two new TKC vectors that show great efficiency in both editing the target gene and in undergoing self-elimination of the transgenes. The new vectors replaced the CaMV35 S promoter used in our previous TKC vector with two rice promoters to drive one of the suicide genes, providing advantages over our previous TKC vector under certain conditions. The vectors reported here offered more options and flexibility to conduct gene editing experiments in rice.

关键词： genome editing suicide gene transgene killer CRISPR Cas9 transgene-free

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Metabolites Composition of Bacillus subtilis HussainT-AMU Determined by LC-MS and Their Effect on Fusarium Dry Rot of Potato Seed Tuber

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Phyton-International Journal of Experimental Botany 2023年第3期92卷 783-799页

作者： Touseef Hussain Abrar A.Khan Heba I.Mohamed plant Pathology and Nematology section Department of BotanyAligarh Muslim UniversityAligarhIndia Biological and Geological Sciences Department Faculty of EducationAin Shams UniversityCairoEgypt

Fusarium dry rot is considered to be the most critical soilborne and postharvest disease that damages potato tubers worldwide when they are stored for a long *** study was performed to demonstrate the effect of crude extract,culture filtrate,and cell suspension obtained from the bacterium Bacillus subtilis Hussain T-AMU,on the net house and *** oil-contaminated soil,through the serial dilution method,biosurfactant bacteria were isolated on nutrient agar *** isolate and screen the prospective biosurfactant strain,various biosurfactant screening methods were *** protocols were carried out for morphological,molecular,and chemical *** disease incidences were significantly decreased under net house and field conditions after treatment with biosurfactant extract(20%,70%),culture filtrate(23%,49%),and bacterial cell suspension(46%,27%),*** results indicate a significant effect of *** HussainT-AMU against Fusarium *** characterization of the partially purified extract by gas-chromatography indicated the existence of a high amount of methyl ester fatty *** quantitative analysis determined the presence of lipopeptide-surfactin,fengycin,and iturin in the biosurfactant *** electron microscopy revealed that morphological variation was revealed in the structure of stomata size in leaves and in the mycelia of the pathogen that was *** promising biocontrol agent can be a plausible option for further research and can also be used for the production of green biomolecules at an additional industrial level.

关键词： Biosurfactant biocontrol agents disease severity lipopeptide stomata

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Potato： A Favorable Crop for plant Molecular Farming

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中国马铃薯 2006年第5期20卷 290-297页

作者： Sunil Kumar G. B Ganapathi T R Bapat V A plant cell culture technology section Nuclear Agriculture and Biotechnology Division Bhabha Atomic Research Centre Trombay Mumbai 400085 India

Potato is one of the important food crops with a high yield potential and nutritional value. It has been used extensively for molecular farming to produce vaccines, antibodies and industrial enzymes. It has several desirable attributes as a favorable crop for the production of recombinant proteins. Potato tubers were employed for bulk production of recombinant antibodies. Vaccine production in potato has progressed to human clinical trials. Human milk proteins were successfully expressed in potato tubers. Potato hairy roots offer as another attractive system for the production of useful recombinant proteins both as intra cellular and secreted forms. This review describes the use of potato as a prospective host for plant molecular farming.

关键词： potato vaccines antibodies hepatitis B microtubers hairy roots

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Study on burrowing nematode, Radopholus similis, pathogenicity test system in tobacco as host

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Journal of Integrative Agriculture 2022年第9期21卷 2652-2664页

作者： YANG Si-hua ZHAO Li-rong DING Sha TANG Shi-qiao CHEN Chun ZHANG Huan-xin XU Chun-ling XIE Hui Research Center of Nematodes of plant Quarantine Laboratory of Plant NematologyCollege of Plant ProtectionSouth China Agricultural UniversityGuangzhou 510642P.R.China technology Center Guangzhou Customs DistrictPeople's Republic of ChinaGuangzhou 510623P.R.China Comprehensive technology and Service Center of Huizhou Port Customs Huizhou 516080P.R.China

Radopholus similis(Cobb 1893) Thorne(1949) is a destructive migratory endoparasitic plant nematode. In this study,the pathogenic process of R. similis infection in Nicotiana benthamiana(tobacco) was studied using quartz sand culture in laboratory. The results showed that R. similis mainly parasitised the root cortex, leading to cortical cell decomposition and tissue decay. We optimised the inoculation conditions to establish a method for determining the pathogenicity of R. similis as follows:(1) a glass culture tube was filled with quartz sand(about 1/3 of the height) and sterilised twice;(2) 20-day-old N. benthamiana seedlings were transplanted into test tubes and cultivated for 10 days at(25±1)°C;(3) R. similis female nematodes were inoculated in the root rhizosphere at a rate of 150 nematodes per plant;(4) the number of nematodes, disease severity, and growth of the plant at 30 days post-inoculation(dpi) were determined. The pathogenicity of eight R. similis populations from different hosts was determined, which proved the feasibility of this method.

关键词： Radopholus similis Nicotiana benthamiana populations pathogenicity

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Efficient CRISPR/Cas9-mediated genome editing in sheepgrass(Leymus chinensis)

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Journal of Integrative plant Biology 2023年第11期65卷 2416-2420页

作者： Zhelong Lin Lei Chen Shanjie Tang Mengjie Zhao Tong Li Jia You Changqing You Boshu Li Qinghua Zhao Dongmei Zhang Jianli Wang Zhongbao Shen Xianwei Song Shuaibin Zhang Xiaofeng Cao State Key Laboratory of plant Genomics and National Center for plant Gene Research Institute of Genetics and Developmental BiologyThe Chinese Academy of SciencesBeijing 100101China Institute of Forage and Grassland Sciences Heilongjiang Academy of Agricultural SciencesHarbin 150086China State Key Laboratory of plant cell and Chromosome Engineering Center for Genome EditingInstitute of Genetics and Developmental BiologyThe Chinese Academy of SciencesBeijing 100101China

The lack of genome editing platforms has hampered efforts to study and improve forage crops that can be grown on lands not suited to other ***,we established efficient Agrobacterium-mediated clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated nuclease 9(Cas9)genome editing in a perennial,stress-tolerant forage grass,sheepgrass(Leymus chinensis).By screening for active single-guide RNAs(sg RNAs),accessions that regenerate well,suitable Agrobacterium strains,and optimal culture media,and co-expressing the morphogenic factor Ta WOX5,we achieved 11%transformation and 5.83%editing efficiency in *** out Teosinte Branched1(TB1)significantly increased tiller number and *** study opens avenues for studying gene function and breeding in sheepgrass.

关键词： Agrobacterium genetic transformation genome editing Lc TB1 sheepgrass

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