检索结果-南通市图书馆

Isolated liver perfusion for regional chemotherapy in rats

Hepatobiliary & Pancreatic Diseases International 2007年第4期6卷 388-392页

作者： Xing, Xue Li, Hong Qingdao Municipal Hosp Dept Hepatobiliary Surg Qingdao 266011 Peoples R China

BACKGROUND: In the isolated liver perfusion (ILP) technique, the liver is temporarily isolated from the systemic circulation in order to infuse large doses of chemotherapeutic agents through the hepatic inflow. The hepatic tissue can show a drug concentration higher than that after systemic chemotherapy, and a general toxic reaction from chemotherapeutic agent is avoided. In a modified ILP model in rats, we explored the distribution of the chemotherapeutic agent, fluorouracil (5-FU) in the systemic circulation and in the hepatic tissue, and assessed the pathological changes in the liver. METHODS: 306 Sprague-Dawley rats were randomly divided into 4 groups: control, ILP without 5-FU, ILP with 5-FU at different doses, and infusion of 5-FU through the portal vein. All animals were subjected to modified ILP, and 5-FU concentrations in the hepatic tissue and the systemic blood were determined at different time points. Levels of thromboxane B-2 (TXB2) and 6-keto-prostaglandin F-1 alpha (6-keto-PGF(1 alpha)), and changes in hepatic function, pathology and liver enzymes were assessed. RESULTS: ILP through the portal vein at three dosages (25, 50 and 100 mg/kg) resulted in significantly higher 5-FU concentrations in the liver tissue for 50 and 100 mg/kg and there was only a small leakage of 5-FU from the perfusion system into the systemic circulation. The pathological findings revealed that the liver tissue tolerated ILP below the maximum tolerance dose (MTD) of 5-FU. If the 5-FU dose exceeded the MTD the ultrastructure and the enzymatic activity of hepatocytes were clearly affected The change of TXB2 and PGF(1 alpha) balance were closely related to the injury of hepatic tissue caused by ILP with 5-FU doses greater than the MTD. CONCLUSIONS: Hepatic tissue sustains a higher drug concentration using ILP than that from systemic chemotherapy, and avoids a general toxic reaction to the drug entering into the systemic circulation. Evident injury may not occur in hepatocytes, and

关键词： liver neoplasm treatment chemotherapy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Endoscopic findings and clinicopathologic characteristics of colonic schistosomiasis:A report of 46 cases

引用

World Journal of Gastroenterology 2010年第6期16卷 723-727页

作者： Cao, Jun Liu, Wen-Jia Xu, Xin-Yun Zou, Xiao-Ping Nanjing Univ Dept Gastroenterol Nanjing Gulou Hosp Sch Med Nanjing 210008 Jiangsu Prov Peoples R China Nanjing Univ Dept Pathol Nanjing Gulou Hosp Sch Med Nanjing 210008 Jiangsu Prov Peoples R China

AIM:To make a retrospective analysis of endoscopy findings and clinicopathologic characteristics of colonic schistosomiasis in order to further improve our understanding of the disease and decrease its misdiagnosis. METHODS:Endoscopy findings and clinicopathologic characteristics of 46 intestinal schistosomiasis patients were retrospectively *** the patients underwent colonoscopy and all biopsy specimens stained with hematoxylin and eosin were observed under a light microscope. RESULTS:Of the 46 colonic schistosomiasis patients,1 was diagnosed as acute schistosomal colitis,16 as chronic schistosomal colitis and 29 as chronic active schistosomal colitis according to their endoscopic findings and *** all patients were suspected of or diagnosed as colonic *** the 12 misdiagnosed patients,4 were misdiagnosed as ulcerativecolitis,1 as Crohn's disease,and 7 as ischemic *** segments of rectum and sigmoid colon were involved in 29 patients(63.0%) .Intact Schistosoma ova were deposited in colonic mucosa accompanying infiltration of eosinocytes,lymphocytes,and plasma cells in acute schistosomal colitis *** fibrosis was found in chronic schistosomal colitis *** the 17 patients with a signal polyp,hyperplastic polyp,canalicular adenoma with a low-grade intraepithelial neoplastic change,tubulovillous adenoma with a highgrade intraepithelial neoplastic change were observed in 10,5,and 2 patients,*** out of the 46 patients were diagnosed as colonic carcinoma. CONCLUSION:Endoscopy contributes to the diagnosis of colonic schistosomiasis although it is nonspecific. A correct diagnosis of colonic schistosomiasis can be established by endoscopy in combination with its clinicopathologic characteristics.

关键词： Colonic schistosomiasis Colonoscopy Diagnosis pathology

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phenol Composition and Susceptibility to Fusarium oxysporum Dianthi in Carnation

引用

Journal of Life Sciences 2011年第11期5卷 921-925页

作者： Francesca Clematis Joseph Tedeschini Marcello Dolci Virginia Lanzotti Benedetta Cangelosil Salvo Fascella Paolo Curir CRA-FSO Corso Inglesi 508 Sanremo 18038 Italy Plant pathology Department University of Tirana Tirana 1001 Albania Di. Va.P.R.A. Chimica Agraria Unito Via Da Vinci 44 Grugliasco 10095 Italy dept. Scienze Alimenti Neaples University Via Universita 100 Portici 80055 Italy

Phenol composition of two carnation （Dianthus caryophyllus） cvs ＂Gloriana＂ and ＂Roland＂, partially and highly resistant to F. oxysporum f. sp. dianthi, respectively, has been investigated with the aim of assessing the possible role of phenolic constituents both as biochemical markers of resistance and antifungal molecules. Analyses were performed through chromatography, NMR and MS methods. The results indicate that protocatechuic and vanillic acids are present in healthy and infected tissues of both cvs; the flavonol glycoside peltatoside. 2,6-Dimethoxybenzoic acid was detected in small amounts only in infected ＂Gloriana＂ cv while the highly resistant ＂Roland＂ cv contained the flavone datiscetin. This latter compound, due to its noticeable antifungal activity evidenced in in vitro trials, could be conceived both as a biochemical marker of resistance towards this pathogen and as a phytoanticipin.

关键词： Dianthus caryophyllus carnation resistance Fusarium oxysporum phenol composition

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Analysis of persistent geotropic and apogeotropic positional nystagmus of the lateral canal benign paroxysmal positional vertigo

引用

Journal of Otology 2022年第2期17卷 90-94页

作者： Sertac Yetiser Dilay Ince Anadolu Medical Center Dept of ORL&HNSKocaeli41400Turkey

Objective:This study aims to analyze the clinical characteristics of persistent geotropic and apogeotropic positional nystagmus of LC-BPPV in view of light and heavy cupula *** and method:The study group includes 184 patients with LC BPPV(98 apogeotropic,86 geotropic type)who have been examined between 2009 and ***-nine females and 85 males,aged between 16 and 92 years were included(Ageotropic 49.32±14.12,geotropic 44.49±13.90 years).Average slow phase velocity(SPV)of positional nystagmus was documented and those with persistent directionchanging positional nystagmus lasting more than a minute were grouped ***,gender difference,side of involvement,and recurrence pattern were particularly ***-square and One way ANOVA tests were used to compare the difference between *** significance was set at P<***:Thirty-seven patients with apogeotropic nystagmus(30.7%;37/98)and 18 patients with geotropic nystagmus(20.9%;18/86)had persistent nystagmus(p?0.05).Comparison of slow phase velocity(SPV)of persistent and non-persistent geotropic and apogeotropic positional nystagmus of the affected side was significant(p?0.05).Comparison of average age,male to female ratio,side of involvement,and the recurrence rate in patients with persistent and non-persistent geotropic and apogeotropic type positional nystagmus groups were not significant(p=0.177,p=0.521,p=0.891,p=0.702).Conclusion:Persistent geotropic and apogeotropic positional nystagmus is mostly correlated with the size,amount,and position of otoconial *** is difficult to justify the light cupula as a new geotropic variant of cupular *** with persistent positional nystagmus present similar therapeutic outcomes and recurrence rates.

关键词： Geotropic Apogeotropic Lateral canal Light cupula Heavy cupula

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Clinicopathologic study of primary intestinal B cell malignantlymphoma

引用

World Journal of Gastroenterology 1999年第6期5卷 538-540页

作者： Zhou Q Xu TR Fan QH Zhen ZX Nanjing Med Univ Affiliated Hosp 1 Dept Pathol Nanjing 210029 Jiangsu Prov Peoples R China

INTRODUCTIONPrimary intestinal B cell lymphoma is one of the most common extra-nodal lymphomas, which includes two types: intestinal mucosa-associated lymphoid tissue lymphoma (IMALToma) and lymphomatous polyposis (LP). Both have characteristic pathologic features, immunophen-otypes and biological behaviors. In this article, twenty-five cases were retrospectively analyzed with regard to criteria of diagnosis and clinicopathologic characteristics.

关键词： lymphoma/pathology intestinal neoplasms/pathology intestinal neoplasms/diagnosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Monitoring the secretion from single cells with temporal and spatial resolution

引用

Chinese Science Bulletin 2000年第4期45卷 289-295页

作者： Weihua Huang Shen Hu Daiwen Pang Zongli Wang Jieke Cheng Wuhan Univ Analyt Sci Ctr Dept Chem & Environm Sci Wuhan 430072 Peoples R China

The secretion from cells plays a significant role in life activites and in the fields of neurobiology, cell biology, clinic, pathology, pharmacology ete. Thus to investigate the secretion at the single cell level with the developing methods is a frontiercross-discipline research activity. The contents, methods and new progress of this research have been reviewed by electrochemical monitoring combined with patch-clamp techniques, fluorescent detection and immunoassay, and the prospects on the frontier of the research area have been discussed.

关键词： single cell secretion neurotransmitters exocytosis electrochemical monitoring fluorescent method.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Commonly used animal models of non-alcoholic steatohepatitis

引用

Hepatobiliary & Pancreatic Diseases International 2009年第3期8卷 233-240页

作者： Fan, Jian-Gao Qiao, Liang Univ Hong Kong Queen Mary Hosp Div Gastroenterol & Hepatol Dept Med Hong Kong Hong Kong Peoples R China Shanghai Jiao Tong Univ Xinhua Hosp Dept Gastroenterol Sch Med Shanghai 200092 Peoples R China

BACKGROUND: Animal models are an essential tool in non-alcoholic steatohepatitis (NASH) studies. Ideally, such models should reflect the etiology, disease progression, and the established pathology of human NASH. To date, no single animal model displays the range of histopathologic and pathophysiologic features associated with human NASH. The currently available models do not or only partially reflect the real picture of human NASH. In particular, insulin resistance and fibrosing steatohepatitis are rarely reproduced by the currently available models. Consequently, it is necessary to establish NASH models that can best mimic the real etiology, disease progression, and pathogenesis of human NASH. DATA SOURCES: We reviewed the major currently available animal models published in the literature (PubMed) and briefly commented on the pros and cons of these models. RESULT: Three major categories of animal models, genetic, dietary, and combination models, were reviewed and discussed. CONCLUSIONS: Animal models are not only useful in revealing the etiology of NASH, but also are important platforms for the assessment of therapeutic strategies. Currently available models do not reflect the full picture of NASH in patients. Better animal models are needed for a full understanding of human NASH and the development of efficient therapies for this condition. (Hepatobiliary Pancreat Dis Int 2009; 8:233-240)

关键词： non-alcoholic fatty liver disease non-alcoholic steatohepatitis animal models

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Fibrodynamics-elucidation of the mechanisms and sites of liver fibrogenesis

引用

World Journal of Gastroenterology 1999年第5期5卷 388-390页

作者： Catherine H. Wu Ph. D.(Department of Medicine,Division of Gastroenterology-Hepatology, University of Connec ticut Health Center, 263 Farmington Avenue, Farmington, Connecticut06030, USA) Univ Connecticut Ctr Hlth Dept Med Div Gastroenterol Hepatol Farmington CT 06030 USA

ORIGINAL ARTICLEDynamic changes of type Ⅰ, Ⅲ and Ⅳ collagen synthesis and distribution of collagen-producing cells in carbon tetrachloride-induced rat liver fibrosis.

关键词： liver cirrhosis/pathology liver cirrhosis/etiology liver cirrhosis/physiopothology fibrodynamics hepatic stellate cells

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

筛选腐霉生防菌的生物测定方法及其效果

引用

湖北农学院学报 1993年第4期13卷 260-265页

作者：张修军 Robert E Beale Annabel Renwick 湖北农学院应用化学所江陵434103 pathology dept NIABCambridgeU K Jealott's Hill Research Station ICI AgrochemicsBracknellU K

设计并比较了在培养皿中利用胡萝卜种子发芽法和温室出苗法测定筛选腐霉生防菌的方法。两方法结果基本一致,培养皿测定有防病或刺激生长作用的菌株表现出高的出苗率。在温室生物测定中,采用营养砂培法培养病原和拮抗真菌以及设计合适的... 详细信息

设计并比较了在培养皿中利用胡萝卜种子发芽法和温室出苗法测定筛选腐霉生防菌的方法。两方法结果基本一致,培养皿测定有防病或刺激生长作用的菌株表现出高的出苗率。在温室生物测定中,采用营养砂培法培养病原和拮抗真菌以及设计合适的拮抗真菌和拮抗细菌施用方法,保证了试验的均一和稳定。在温室病原诱发接种条件下,发病表现为出苗率的差异,即丧失发芽能力或出苗前的猝倒.病原接种量越高,出苗率越低.在测定的4个真菌菌株,16个细菌菌株中,发现两个细菌菌株防病保苗效果突出,在高病原接种量对照出苗率29%的情况下,出苗率达70%以上.另有多个菌株表现亦不错.

关键词：腐霉生防菌生物测定方法

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

检测冰冻肝组织切片中丙型肝炎病毒RNA的一种快速和高度特异性方法

引用

德国医学 1997年第1期14卷 14-17页

作者： C.Sergi T.Goeser G.Otto H.F.Otto W.J.Hofmann 杨燕 dept.of pathology.University of Heidelberg Im Neuenheimer Feld 220/221D-69120 HeidelbergGermany 同济医科大学附属同济医院

目的:建立一种简单适用的以多聚酶链式反应(PCR)为基础的方法,以便快速、高度特异性地从单份冰冻组织切片提取的RNA中检测HCV核酸。方法:对18例施行原位肝移植患者的肝组织进行HCV RNA的检测,其中9名患者有HCV抗原血症(EIA-2,RIBA-2)和... 详细信息

目的:建立一种简单适用的以多聚酶链式反应(PCR)为基础的方法,以便快速、高度特异性地从单份冰冻组织切片提取的RNA中检测HCV核酸。方法:对18例施行原位肝移植患者的肝组织进行HCV RNA的检测,其中9名患者有HCV抗原血症(EIA-2,RIBA-2)和病毒血症(PCR),另9名正常。从每名患者标本的组织切片(1～10μm)中抽提出总RNA,一部分采用常规的逆转录套式PCR(RT-nestded PCR)进行检测,包括引物特异的逆转录和套式RCR,两对寡核苷酸引物来自于HCV RNA 5′端非编码区。另一部分采用组合RT-nested PCR检测,即逆转录与第一次PCR在同一离心管中进行,整个过程只需5小时。管中用以隔开逆转录酶(RTase)与Taq DNA聚合酶的石蜡界面可有可无。验证试验采用斑点印迹杂交,能够与地高辛(DIG)标记的寡核苷酸探针进行杂交的标本为HCV阳性。结果:运用常规的分步RT-PCR与组合RT-PCR两种检测方法,发现9例HCV阳性患者其肝组织中HCV RNA为阳性,9例外周血中HCV阴性患者其肝组织中HCV RNA亦为阴性。少至500ng的肝组织总RNA作为模板也能产生在EB染色的琼脂糖凝胶上可检测到的cDNA扩增产物。DIG标记的Alx-89寡核苷酸探针仅与HCV RNA阳性标本杂交。结论:组合RT-PCR能够在一种通用缓冲体系中一步完成逆转录和第一次PCR,高度特异性地直接检测肝组?

关键词：肝移植肝组织切片丙型肝炎病毒 RNA

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：