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Differential gene expression between squamous cell carcinoma of esophageus and its normal epithelium; altered pattern of mal,akrlc2, and rablla expression

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World Journal of Gastroenterology 2004年第12期10卷 1716-1721页

作者： Sakineh Kazemi-Noureini Sergio Colonna-Romano Abed-Ali Ziaee Mohammad-Ali Malboobi Mansour Yazdanbod Parviz Setayeshgar Bruno Maresca Institute of Biochemistry and Biophysics University of TehranTehranIran PO Box:13145-1384 International Institute of Genetics and Biophysics National Research CouncilVia Marconi 1280125 NaplesItaly National Research Center for Genetic Engineering and Biotechnology(NRCGEB) TehranIran Shanati Hospital Department of SurgeryMedical University of TehranTehranIran pathology department Madaen HospitalTehranIran and University of Salerno School of PharmacyDept.Pharmaceutical SciencesFiscianoSalernoItaly

AIM: To identify the altered gene expression patterns in squamous cell carcinoma of esophagus (ESCC) in relation to adjacent normal esophageal epithelium. METHODS: Total RNA was extracted using SV total RNA isolation kit from snap frozen tissues of ESCC samples and normal esophageal epithelium far from the tumor. Radio-labeled cDNA were synthesized from equal quantities of total RNAs of tumor and normal tissues using combinations of 24 arbitrary 13-mer primers and three different anchoring oligo-dT primers and separated on sequencing gels. cDNA with considerable different amounts of signals in tumor and normal tissue were reamplified and cloned. Using southern blot, the clones of each band were controlled for false positive results caused by probable heterogeneity of cDNA population with the same size. Clones that confirmed differential expression by slot blot selected for sequencing and northern analysis. Corresponding full-length gene sequences was predicted using human genome project data, related transcripts were translated and used for various protein/motif searches to speculate their probable functions. RESULTS: The 97 genes showed different levels of cDNA in tumor and normal tissues of esophagus. The expression of mal gene was remarkably down. regulated in all 10 surveyed tumor tissues. Akrlc2, a member of the aldoketo reductase 1C family, which is involved in metabolism of sex hormones and xenobiotics, was up-regulated in 8 out of 10 inspected ESCC samples. Rablla, RPL7, and RPL28 showed moderate levels of differential expression. Many other cDNAs remained to further studies. CONCLUSION: The mal gene which is switched-off in all ESCC samples can be considered as a tumor suppressor gene that more studies in its regulation may lead to valuable explanations in ESCC development. Akrlc2 which is upregulated in ESCC probably plays an important role in tumor development of esophagus and may be proposed as a potential molecular target in ESCC treatments. Differentia

关键词：基因表达鳞状细胞癌上皮细胞家兔 ESCC 食管

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FgCDC14 regulates cytokinesis,morphogenesis,and pathogenesis in Fusarium graminearum

FgCDC14 regulates cytokinesis,morphogenesis,and pathogenesis...

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中国植物病理学会2015年学术年会

作者： LI Chao-hui ZHANG Shi-jie Michael Melesse HAO Chao-Feng WANG Chen-fang Mark C.Hall XU Jin-Rong State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest Agricultural and Forestry University dept.of Botany and Plant pathology Purdue University dept.of Biochemistry Purdue University

Members of Cdc14 phosphatases are common in animals and fungi but absent in *** its orthologs are conserved in plant pathogenic fungi,their functions during infection are not *** this study,we showed that CDC14 is important for pathogenesis and morphogenesis in two fungal *** Fusarium graminearum,FgCDC14 is required for normal cell division and septum formation and FgCdc14 possesses phosphatase activity with specificity for a subset of yeast Cdk-type phosphorylation *** Fgcdc14 mutant was reduced in growth,conidiation,and ascospore *** was defective in ascosporogenesis and *** in Fgcdc14 was reduced and hyphal compartments contained multiple nuclei,indicating defects in the coordination between nuclear division and ***,foot cells of mutant conidia often differentiated into conidiogenous cells,resulting in the production of inter-connected *** the interphase,FgCdc14-GFP localized to the nucleus and *** ***,deletion of MoCDC14 resulted in similar defects in growth,conidiation,and *** Mocdc 14 mutant was defective in appressorium morphogenesis and *** together,our results indicate that Cdcl4 phosphatase functions in cell division and septum formation in *** and ***,likely by counteracting Cdk phosphorylation,and is required for plant infection.

关键词： Cell cycle Cytokinesis Plant Infection Conidiation Sexual reproduction Gibberella zeae

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FgSsn3 kinase,a component of the mediator complex,is important for sexual reproduction and pathogenesis in Fusarium graminearum

FgSsn3 kinase,a component of the mediator complex,is importa...

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中国植物病理学会2015年学术年会

作者： CAO Shu-lin ZHANG Shi-jie LIU Hui-quan HAO Chao-feng JIN Qiao-jun XU Jin-rong State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest Agricultural and Forestry University dept.of Botany and Plant pathology Purdue University

Fusarium graminearum,an important plant pathogen of wheat and barley,not only causes severe yield losses but also produces harmful mycotoxins contaminating infested *** this study,we characterized the FgSSN3 gene in *** which is orthologous of SSN3 in Saccharomyces cerevisiae,SRBIO in Saccharomyces pombe and Neurospora *** Fgssn3 deletion mutant is decreased in vegetative growth,abnormal colony appearance and significant reduced in virulence on wheat heads and maize *** and transmission electron microscopy（SEM and TEM） results indicate that the mutant Fgssn3 failed to spread from the inoculation site to other parts of wheat *** it is defective in conidium morphology,the Fgssn3 is normal in *** of FgSSN3 resulted in increased expression levels of HTF1 and *** addition,the Fgssn3 mutant is defective in sexual reproduction and essential for female fertility but dispensable for male ***3 physically interacted with FgSsn8 in yeast two-hybrid *** indicate that FgSsn3 as a subunit of mediator complex in *** regulates growth,development,sexual reproduction and plant infection and contributes to transcriptional repression or activation of diversely regulated genes.

关键词： Mediator complex Plant Infection Conidiation Sexual reproduction Gibberella zeae

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Environmental toxicology and exposure to natural dust: The role of trace elements

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Chinese Journal Of Geochemistry 2006年第B8期25卷 222-223页

作者： Jose A. Centeno Angus Cook Phil Weinstein dept. of Environrnnetal and Infectious Disease Sciences US Armed Forces Institute of Pathology Washington DC USA 20306-6000 Ecology and Health School of Population Health Western Australia University Perth Western Australia Australia

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耐多药结核病的研究及进展

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中华结核和呼吸杂志 2000年第2期23卷 119-123页

作者：潘晞何国钧 Section of Tuberculosis Dept.of Molecular PathologyBaylor Medical College 上海医科大学呼吸病研究所上海市肺科医院200433

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HER2在结直肠癌中的表达及意义

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中国老年学杂志 2010年第10期30卷 1407-1409页

作者：张凯王中义 David Zhang 李春生李红刘铜军吉林大学中日联谊医院结直肠肛门外科吉林长春130033 Molecular pathology dept.Mount sinai Hospital New York U.S.

目的研究HER2在结直肠癌的表达及意义。方法应用pathway array analysis方法分析了56例结直肠癌肿瘤组织及癌旁正常组织中HER2的表达。结果在56例结直肠癌患者中共发现HER2阳性表达35例,21例正常黏膜组织中HER2过表达,其中16例结直肠癌... 详细信息

目的研究HER2在结直肠癌的表达及意义。方法应用pathway array analysis方法分析了56例结直肠癌肿瘤组织及癌旁正常组织中HER2的表达。结果在56例结直肠癌患者中共发现HER2阳性表达35例,21例正常黏膜组织中HER2过表达,其中16例结直肠癌患者有淋巴结转移。结论 HER2的表达与结直肠癌的发生发展相关,检测HER2的表达可作为判断结直肠癌的生物学行为和预后的重要指标。

关键词：结直肠癌 HER2 pathway array

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Effects of Streptomyces microflavus Extract on Heat Tolerance of Tall Fescue

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Journal of Shanghai Jiaotong university(Science) 2005年第S1期10卷 19-25页

作者：王兆龙殷朝珍孙吉雄黄炳如 School of Agriculture &Biology Shanghai Jiaotong Univ. Shanghai 201101 China College of Biology &Biotechnology Yangzhou Univ. Yangzhou 225009 dept. of Plant Biology &pathology Rutgers Univ. New Brunswick NJ 08901 USA

Heat stress in summer is a major factor limiting use of cool-season grasses in Shanghai area, China. The objectives of this study were to investigate effects of a crude extract product of a new strain of Streptomyces microflavus (TSS) on turf performance and physiological activities of tall fescue (Festuca arundinacea) in response to heat stress. Plants of tall fescue cultivar ‘Barlexus’ were exposed to 38/33 (°C) (day/night) high temperature in growth chamber after TSS applications. High temperature induced about 3.5 fold increases of initial shoot extension rate and clipping yield (6 d) and led to quick decline of plant growth after 18 d. TSS inhibited the extent of initial increases of shoot extension rate and clipping yield and maintained a longer period of steady growth under the heat stress. TSS also decreased the decline of leaf chlorophyll content, TNC, shoot density, and turf quality induced by heat stress. The results suggested that TSS application improved turf performance under heat stress, and the greater improvement of heat tolerance was associated with higher dose of application. This positive effect on heat tolerance could be related to the decreased carbohydrate consumption and loss, and reduced decomposition of leaf chlorophyll.

关键词： heat tolerance Streptomyces microflavus extract tall fescue carbohydrate consumption

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应用流式细胞计对人周围血及骨髓细胞的分析

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解剖学报 1990年第S1期21卷 87-93页

作者：陆明 Nicola M.Kouttab 重庆医科大学组织胚胎学教研室重庆630046 dept of pathology Roger Williams General HospitalBrown UniversityProvidence.RI.U.S.A.

本研究应用流式细胞计(Flow Cytometry,下称流式计)及与淋巴细胞、髓细胞反应的单克隆抗体(下称单抗),对正常人周围血淋巴细胞及其亚群、艾滋病血细胞的变化以及急性白血病骨髓细胞的免疫表型进行了分析。得到正常血中CD_2、CD_3、CD_4... 详细信息

本研究应用流式细胞计(Flow Cytometry,下称流式计)及与淋巴细胞、髓细胞反应的单克隆抗体(下称单抗),对正常人周围血淋巴细胞及其亚群、艾滋病血细胞的变化以及急性白血病骨髓细胞的免疫表型进行了分析。得到正常血中CD_2、CD_3、CD_4、CD_8、CD_(19)、CD_(20)及CD_(21)细胞的百分率以及CD_2、CD_3、CD_4、CD_8细胞的绝对值。CD_4/CD_8细胞的比值平均为1.83±0.36(n=40)。而艾滋病人CD_4细胞明显下降:百分率由47％降至15—25％,绝对值由784降至175—344/mm^3。CD_4/CD_8的比值平均为0.68±0.24(n=6,P<0.01),与正常人相比明显下降。CD_4细胞是艾滋病诊断和预后的重要指标。急性淋巴性白血病(ALL)被T_(11)、Leu_9和有非T-ALL病例。B_4、B_1是B-ALL的决定性标记物。Cu阳性细胞比SmlgG阳性细胞更幼稚,为早期B细胞。标记髓细胞的单抗My4、My7、My9、Mo1和Mo2被用于区别急性髓性白血病(AML)及ALL。17例AML均表现My9,其中6例还同时表现Mo2及My4,被视为单核细胞型。双染流式计分析表明,毛细胞白血病(HCL)同时表现与B细胞反应的Leu14及与单核细胞反应Mo5。这个特性可以作为诊断HcL的又一指标。本研究将流式计应用于临床诊断和免疫监护,对其使用方法的规范化及仪器的标准化进行了讨论。

关键词：流式细胞计 T 细胞亚群艾滋病免疫表型

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Suppressing progress of pancreatitis through selective inhibition of NF-κB activation by using NAC

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Journal of Zhejiang University Science 2004年第4期5卷 477-482页

作者：赵志成郑树森陈文亮王选齐莹 dept ofHepatobiliarySurgeryFirstAffiliatedHospitalMedicalCollegeZhejiangUniversityHangzhou310003China dept.ofSurgery SecondAffiliatedHospitalShanxiMedicalUniversityTaiyuan030001China

Objective: To explore the characteristics of NF-闎 activation in the progress of pancreatitis, the relationship with expression of TNF- in the inflammatory reaction, and prevent the exacerbation of pancreatitis by using NAC. Method: Forty-eight rats were divided into three groups: therapy (group C), pancreatitis (group B) and control (group A). NAC served as the inhibitor of NF-闎 activation. In the time intervals of 1.5, 3.0, 6.0, 12.0 hour, NF-闎 activation was detected with flow cytometry (FCM) and the expression of TNF- mRNA and protein with in situ hybridization (ISH) and enzyme-linked immuno-sorbent assay (ELISA) respectively. Meanwhile, the level of lipase and amylase in the serum was assayed and the pathological change was evaluated. Result: NF-闎 activation in the pancreatitis group was higher than that in the control group (Ppathology changes of the pancreas were shown to be alleviated in group C. Conclusion: First, NF-闎 activity is intensively initiated in the course of pancreatitis and shown to have closely relationship with the release of cytokines. Second, use of NAC markedly depressed NF-闎 activation. TNF- expression is down regulated by cytokines. It is suggested that NAC probably acts as a useful agent for treatment of pancreatitis by indirectly inhibiting activation of NF-闎.

关键词： Pancreatitis NF-κB TNF-α N-acetycysteine Cytokine

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Genome-wide A-to-I RNA editing during sexual reproduction in filamentous ascomycetes

Genome-wide A-to-I RNA editing during sexual reproduction in...

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中国菌物学会2016年学术年会

作者： Huiquan Liu Yang Li Jin-Rong Xu Purdue-NWAFU Joint Research Center State Key Laboratory of Crop Stress Biology for Arid AreasCollege of Plant ProtectionNorthwest A&F University dept.of Botany and Plant pathology Purdue University

＜正＞Yeasts and filamentous fungi do not have Adenosine Deaminase Acting on RNA（ADAR）orthologs and are believed to lack A-to-I RNA editing,which is the most prevalent m RNA editing m RNA in ***,we recently found that A-to-I editing occurs specifically during sexual reproduction in the wheat scab fungus Fusarium ***-wide analysis identified 26,056 perithecium-specific A-to-I editing *** those in animals,

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