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抗虫转基因欧洲黑杨的培育

生物工程学报 1993年第4期9卷 291-297页

作者：田颖川李太元莽克强韩一凡李玲王学聘卢孟柱戴连韵韩一侬严静君 W.Gaberiel 中国科学院微生物研究所中国林业科学院林业研究所 dept. of Plant Pathoigy University of Florida Gainesville

用带有35S-Ω-Bt-NOS嵌合基因的双元载体的农杆菌LBA 4404转化欧洲黑杨的叶片外植体,共获得54株转化再生植株。用这些再生植株对杨尺蠖(Apochimia cinerarius)进行毒力测定,昆虫校正死亡率在80—96%的再生植株占总测定植株的15%。部分... 详细信息

用带有35S-Ω-Bt-NOS嵌合基因的双元载体的农杆菌LBA 4404转化欧洲黑杨的叶片外植体,共获得54株转化再生植株。用这些再生植株对杨尺蠖(Apochimia cinerarius)进行毒力测定,昆虫校正死亡率在80—96%的再生植株占总测定植株的15%。部分再生植株对舞毒娥进行测定,表明在5—9天内校正死亡率高达100%,存活昆虫的生长和发育也明显地受到抑制。根据苗木在苗圃中高生长和昆虫校正死亡率采用重心聚类法进行分析,初步选出高生长良好和杀虫率居中的3株再生值株。以选出的植株为主进行了PCR及PCR产物的Southern blot和再生植株DNA Southern blot测定,结果证明Bt基因已插入到这些植株的DNA上,并表达出苏云金杆菌杀虫蛋白的杀虫活性。

关键词：杨树 Bt基因抗虫

The expression and functional mechanism of long non-coding RNA LINC00339 in colorectal cancer

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China Medical Abstracts(Internal Medicine) 2019年第3期36卷 168-168页

作者： FU Qiang dept Gastrointestin Surg Henan Tumor Hosp

Objective To investigate the expression of long noncoding RNA LINC00339 in colorectal cancer patients and its effect and mechanism on proliferation and apoptosis of colorectal cancer *** A retrospective analysis of 158 pathology-confirmed colorectal cancer patients,who were enrolled from August 2015 to January 2017,was ***00339 expression in colorectal cancer tissues and adjacent colorectal samples were detected by Real-time *** correlation between LINC00339 expression and clinicopathological features as well as the relationship between LINC00339 and microRNA(miR)-218 expression was *** interaction between LINC00339 and miR-218 was further confirmed by dual luciferase report *** of LINC00339 was performed by siRNA interference technology in LoVo and HCT116 ***-time PCR was used to detect miR-218 expression.3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT)analysis was carried out to examine cell *** cytometry was used to determine cell ***,LINC00339 siRNA and miR-218 antagomirs(anti-miR-218)were co-transfected into LoVo and HCT116 cells,and then cell viability and apoptosis were *** LINC00339 expression was significantly increased in colorectal cancer tissues compared with adjacent colorectal tissues(4.69±1.52 vs 1.02±0.38,P0.05),but was associated with TNM stage,lymphatic metastasis,tumor maximum diameters,and differentiation degree(all P<0.05).LINC00339 expression was negatively correlated with miR-218 expression in colorectal cancer tissues(P<0.05).miR-218 mimics remarkably suppressed the fluorescence intensity of wild-type LINC00339 plasmid(P=0.001),but did not affect the fluorescence intensity of the mutant ones(P=0.88).Knockdown of LINC00339 remarkably inhibited proliferation,but promoted apoptosis of LoVo and HCT116 cells(all P<0.05).Compared with cells transfec

关键词： HCT116 colorectal cancer

Relationship between estrogen receptor α and β expression and clinicopathological characteristics in elderly patients with non-small cell lung cancer

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China Medical Abstracts(Internal Medicine) 2013年第2期30卷 95-95页

作者：李琳 dept Oncol Beijing Hosp Health Ministry

Objective To evaluate estrogen receptor(ER)α and β expressions in elderly patients with non-small cell lung cancer(NSCLC) in China and their relationship with clinical and pathological

关键词： estrogen and elderly statistically histology correlate nucleus pression pathology stronger

Spectrum of children’s kidney diseases—9925 cases proven by renal biopsy from a single center of China between 2004 and 2017

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China Medical Abstracts(Internal Medicine) 2019年第2期36卷 114-114页

作者： GAO Chunlin Pediatric dept Jinling HospNanjing 210002

Objective To analyze the spectrum of children'skidney pathology by renal biopsy. Methods The clinicaland pathological data of the cases in Jinling Hospital involvingpatients younger than 18 years old who receivedrenal biopsy from April 1st,2004 to December 31th,2017 were retrospectively collected,and compared withthe renal pathological data of 1611 children aged0 - 18 years from June 1982 to March 2004. ResultsThis study included 9925 cases of kidney diseases provenby renal biopsy. The ratio of male to female was 1. 79∶ *** glomerulonephritis ( PGN ) accounted for66. 14%,and secondary glomerulonephritis ( SGN) accountedfor 28. 00%. Top five of the PGN were IgA nephropathy( IgAN,19. 11%),mesangial proliferativeglomerulonephritis ( MsPGN, 16. 07%), minimalchange disease (MCD,14. 20%),focal segmental glomerulosclerosis( FSGS,6. 19%) and membranous nephropathy(MN,4. 70%),IgAN (13. 12%),MsPGN(11. 20%),MCD (10. 63%),FSGS (4. 55%) andMN (2. 54%) in males,and IgAN (5. 99%),MsPGN(4. 87%),MCD (3. 57%),MN (2. 16%) and FSGS(1. 63%) in females.

关键词： Spectrum of children’s kidney diseases cases proven China

多系统萎缩和帕金森病患者半固态饮食后的胃排空

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世界核心医学期刊文摘(神经病学分册) 2006年第1期 20-21页

作者： Thomaides T. Karapanayiotides T. Zoukos Y. 李一明 dept. of Neurology Pammakaristos Hospital 43 Iakovaton Str. 11144 Athens Greece Dr.

Background: Gastrointestinal symptoms such as nausea, abdominal pain, and bloating are frequent complaints in patients with Parkinson disease (PD) and in patients with multiple system atrophy (MSA), and may be associated with delayed gastric emptying (GE). Although several GE studies in patients with PD have been performed, scant data exist in patients with MSA. Methods: We assessed GE half-times (T50) in 12 patients with MSA and compared them with those of 12 patients with PD and 12 age-matched healthy *** was estimated scintigraphically using the left anterior oblique method after ingestion of a 99mTc colloid-labeled balanced semi-solid meal (yogurt).GE data were obtained every 15 minutes until there was complete emptying of the stomach. Blood pressure, heart rate, plasma glucose and glucosylated hemoglobin were regularly determined. Results: Reproducibility of the GE technique was excellent (Bland-Altman analysis, limits of agreement: -2.3 to 2.8). T50 was longer in MSA (82 ±3.4 min) and in PD (90.6 ±3.9 min) patients compared with controls (46.2 ±0.7) (two way ANOVA, p 0.05). Conclusions: Our results suggest that patients with MSA have GE rates similar to those of patients with PD, but slower than healthy agematched individuals. It remains to be investigated whether gastrointestinal dysfunction in MSA is related to both brain and peripheral pathology, as is presumed for PD.

关键词：帕金森病胃排空多系统萎缩脑病患者

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血压、白质损伤和颞叶内侧萎缩之间的联系

世界核心医学期刊文摘（神经病学分册） 2005年第6期1卷 42-43页

作者： Den Heijer T. Launer L.J. Prins N.D. M.M.B. Breteler 邓剑平 dept. of Epidemiol. and Biostatist. Erasmus MC 3000 DR Rotterdam Netherlands Dr.

Background: Blood pressure level is associated with the risk of clinical Alzheimer disease (AD), yet the underlying mechanisms are unclear. High blood pressure levels may cause cerebral small- vessel pathology, which contributes to cognitive decline in patients with AD. Alternatively, in persons with high blood pressure, increased numbers of neurofibrillary tangles and amyloid plaques at autopsy have also been observed, suggesting direct links between blood pressure and AD. Abstract:Objective: To investigate the association of blood pressure and markers of small- vessel disease (white matter lesions [WMLs] on MRI)- with hippocampal and amygdalar atrophy on MRI - potential in vivo indicators of Alzheimer pathology. Methods: In 1995 to 1996, 511 nondemented elderly subjects (age 60 to 90) underwent MRI. The extent of WMLs was assessed, and volumes of the hippocampus and amygdala were measured. Blood pressure levels were assessed at the time of MRI and 5 years before the MRI. Results: Higher diastolic blood pressure 5 years before MRI predicted more hippocampal atrophy in persons untreated for hypertension (per SD increase - 0.10 mL [95% CI - 0.19 to - 0.02, p = 0.02]). Conversely, in persons treated for hypertension, a low diastolic blood pressure was associated with more severe atrophy. Persons with more WMLs on MRI more often had severe atrophy of the hippocampus and amygdala. Conclusion: Blood pressure and indicators of small- vessel disease in the brain may be associated with atrophy of

关键词：颞叶内侧神经纤维缠结阿尔茨海默病小血管病变杏仁核淀粉样变潜在机制

轻度胎儿肾盂扩张的产前诊断和处理:新生儿并发症的结局及随访

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世界核心医学期刊文摘（妇产科学分册） 2005年第6期1卷 29-30页

作者： Signorelli M. Cerri V. Taddei F 王颖 Prenatal Diagnosis Unit Dept. of Obstetrics and Gynecology Univ. of Brescia Spedali Civili Brescia Italy

Objectives: To describe the natural history of pyelectasis from its detection in the second trimester to delivery, its capability to predict renal pathology and whether prenatal development of pelvic dilatation is correlated to its postnatal evolution. Study design: A retrospective analysis involving 375 fetuses with a complete urological follow- up. Prenatal ultrasound was correlated with the results of postnatal investigation and frequency of postnatal surgery was established. Results: Two- hundred and eighty cases underwent at least two examinations before birth. 73.1% were male fetuses. 57.4% had a bilateral pyelectasis. Prenatal evolution of pelvic dilatation was the following:18.6% of the cases normalized, in 34.6% of the cases the dilatation reduced but did not disappear, in 30.7% it remained unchanged, while it worsened in 16.4% . One case from the first group, three cases from the second, seven cases from the third and 11 cases from the fourth needed surgical treatment. 1.9, 7.2, 18.6, 23.9% of cases respectively worsened after birth in the four groups (trend: P=0.001). Conclusions: Prenatal diagnosis of pyelectasis improves the outcome of these children due to a surgical approach that avoids renal damage. There is a good correlation between prenatal evolution and postnatal outcome, although a postnatal follow- up is opportune in those cases that normalized before birth.

关键词：肾盂扩张产前诊断新生儿并发症产前超声外科手术产后随访妊娠中期泌尿科产后病研究设计

接受每日维生素A补充治疗的囊性纤维化患者与眼球表面变化相关的视黄醇结合蛋白状态

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世界核心医学期刊文摘（儿科学分册） 2005年第9期1卷 39-40页

作者： Mrugacz M. Tobolczyk J. Minarowska A. 高蕊 dept. of Paediatric Ophthalmology Medical University of BialystokWashington Street 17 15-274Bialystok Poland

Cystic fibrosis (CF) is an autosomal recessive disease characterised by increased viscosity of mucus secretions and high chloride concentration in exocrine secretions. Clinically, the patients suffer from chronic pulmonary changes, chronic pancreatic deficiency, and an obstruction of the gastrointestinal tract. The disease affects all secretory epithelia including the eye. The influence of nutritional status on long-term survival and quality of life of CF patients is well documented. Steatorrhea, a consequence of decreased fat digestion and absorption may be associated with vitamin deficiences, including vitamin A. The aimof this study was to document plasma retinol binding protein (RBP) status, a specific plasma transport protein for vitamin A, and ocular surface changes in children and adolescents with CF. The patients were recruited at the 3rd Department of Paediatric Diseases, Medical University of Bialystok, Poland. All patients were regularly seen by a CF specialist dietitian. A group of 15 patients had the following investigations: plasma RBP, visual acuity, physical examination, tear film break-up time, fluorescein staining and Schirmer tear test. A group of 15 age-and sex-matched controls without CF or ocular pathology were also recruited. Plasma RBP concentrations were significantly lower in patients with CF than in the control group. CF patients showed a statistically significant increase in the incidence of clinical blepharitis. Five of the CF patients had clinical evidence of dry eyes. Conclusion:Low plasma retinol binding protein levels frequently occur in clinically stable and retinol supplemented cystic fibrosis patients, of whom five had dry eyes. We recommend monitoring of plasma retinol binding protein levels and evaluation of ocular surface changes, especially those with dry eye symptoms in all cystic fibrosis patients.

关键词：视黄醇结合蛋白补充治疗囊性纤维化维生素缺乏远期存活率转运蛋白特异性血清黏液分泌脂肪痢营养状况

α-共核蛋白异常不能预测锥体外系症状或痴呆

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世界核心医学期刊文摘（神经病学分册） 2005年第5期1卷 14-15页

作者： Parkkinen L. Kauppinen T. Pirttil T. I. Alafuzoff 郭俊 dept. of Neurosc ience and Neurology Section of Neuropathology Kuopio University PO Box 1627 70211 Kuopio Finland

Intracytoplasmic aggregation of α  synuclein protein as Lewy bodies in the brainstem neurons is diagnostic for Parkinson s disease, whereas if this proc ess also occurs in the cortical neurons, it is considered pathognomonic for deme ntia with Lewy bodies. However, the link between α synuclein incorporation into inclusions, neuronal dysfunction, and clinical symptoms needs to be clarified. Another important issue of the pathogenetic puzzle is to understand where α  synuclein pathology begins and how it progresses in the brain. To study this, we collected all cases from autopsy material (N = 904) that had α  synuclein pa thology in the dorsal motor nucleus of vagus,substantia nigra, and/or basal fore brain nuclei. In this way, our study has a unique design because the selection o f material is entirely based on the presence of α  synuclein pathology regard less of clinical phenotype. Retrospective clinical assessment then showed that o nly 32 (30% ) of 106 α  synuclein positive cases were diagnosed with a neu rodegenerative disorder. The distribution or load of α  synuclein pathology d id not permit a dependable postmortem diagnosis of extrapyramidal symptoms or co gnitive impairment. Some neurologically unimpaired cases had a reasonable burden of α  synuclein pathology in both brainstem and cortical areas, suggesting t hat α  synuclein positive structures are not definite markers of neuronal d ysfunction.

关键词：锥体外系症状帕金森病黑质基底前脑皮层神经元认知功能损害共核神经元功能脑干神经元细胞质内