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Suppressor of RNA silencing encoded by Rice gall dwarf virus genome segment 11

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Chinese Science Bulletin 2008年第3期53卷 362-369页

作者： LIU FuXiu ZHAO Qin RUAN XiaoLei HE YunWei LI HuaPing Laboratory of Plant Virology South China Agricultural University Guangzhou 510642 China Post-entry quarantine Station for Tropical Plant Hainan Entry-Exit Inspection and Quarantine Bureau Haikou 570206 China

Rice gall dwarf virus(RGDV)is an important rice pathogen in China and Southeast ***,little is known about the molecular mechanisms of RGDV interactions with plant ***,we have identi-fied an RGDV protein,Pns11,which acts as a suppressor of RNA silencing in coinfiltration assays with the reporter,green fluorescent protein(GFP)in transgenic Nicotiana benthamiana line 16c carrying ***11 suppressed local and systemic silencing induced by sense *** spread of mobile RNA si-lencing signals was blocked or inactivated by *** of Pns11 also enhanced Potato virus X pathogenicity in *** suppressor could reduce,but not eliminate,siRNA in the local and systemic RNA silencing suppression assays,suggesting that Pns11 functions by interfering with initial stages of RNA silencing.

关键词：水稻矮化病毒 RNA噪声抑制抑制物基因片段

Preparation and Structural Analysis of Lanthanide Complexes with α-Hydroxyl-phenyl-acetic Acid and 1,10-Phenanthroline as Ligands

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Journal of Donghua University(English Edition) 2008年第3期25卷 340-344页

作者：林苗严素杨勇潘文丽 College of Chemistry Chemical Engineering and BiotechnologyDonghua University inspection Center of Industrial Product & Raw Materials Shanghai Entry-Exit Inspection & Quarantine Bureau

A series of ternary lanthanide complexes Ln （C6H5CHOHCOO）3（phen）（H2O）（Ln= La, Ce, Pr, Nd, Sm, Eu） were synthesized with α-hydroxyl-phenyl-acetic acid and 1, 10-phenanthroline （phen） as ligands. The sturcture and property of complexes were characterized by elemental analysis, IR, 1^H-NMR, UV and TGA, and the coordinate mede of both ligands was discussed. It was shown that the co-ordinate mode of 1, 10-phenanthroline was chelated, while α-hydraxyl-phenyl-acetate ion was monedentate or bridging. The mechanism of thermal determinde by thermogravimetry was indicated that the degree of co-ordinate ability of ligand was： α-hydroxyl-phenyl-acetate ion 〉 1, 10- phenanthroline（phen）.

关键词： lanthanide ternary complex α-hydroxyl-phenyl-acetic acid 1, 10-phenanthroline structural analysis

Rice Straw-Derived Biochar Properties and Functions as Cu(Ⅱ) and Cyromazine Sorbents as Influenced by Pyrolysis Temperature

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Pedosphere 2015年第5期25卷 781-789页

作者： JIANG Jun Yongbo PENG YUAN Min HONG Zhineng WANG Dejian XU Renkou State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of Sciences Indiana University Jiangsu entry-exit inspection and quarantine bureau

In this study, biochars from rice straw （Oryza sativa L.） were prepared at 200-600 ℃ by oxygen-limited pyrolysis to investigate the changes in properties of rice straw biochars produced at different temperatures, and to examine the adsorption capacities of the biochars for a heavy metal, copper（II）（Cu（II））, and an organic insecticide of cyromazine, as well as to further reveal the adsorption mechanisms. The results obtained with batch experiments showed that the amount of Cu（II） adsorbed varied with the pyrolysis temperatures of rice straw biochar. The biochar produced at 400 ~C had the largest adsorption capacity for Cu（II）（0.37 mol kg-1） among the biochars, with the non-electrostatic adsorption as the main adsorption mechanism. The highest adsorption capacity for cyromazine （156.42 g kg-1） was found in the rice straw biochar produced at 600 ℃, and cyromazine adsorption was exclusively predominated by surface adsorption. An obvious competitive adsorption was found between 5 mmol L-1 Cu（II） and 2 g L-1 cyromazine when they were in the binary solute system. Biochar may be used to remediate heavy metal- and organic insecticide-contaminated water, while the pyrolysis temperature of feedstocks for producing biochar should be considered for the restoration of multi-contamination.

关键词： adsorption capacity adsorption mechanism desorption heavy metal multi-contaminated water non-electrostatic adsorption organic insecticide surface charge

The recognition of ocean red tide with hyper-spectral-image based on EMD

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Chinese Journal of Oceanology and Limnology 2008年第2期26卷 137-141页

作者：赵文仓韦洪丽时长江姬光荣 College of Automation and Electronic Engineering Qingdao University of Science & Technology Shandong entry-exit inspection and quarantine bureau Department of Electronic Engineering Ocean University of China

A new technique is introduced in this paper regarding red tide recognition with remotely sensed hyper-spectral images based on empirical mode decomposition （EMD）, from an artificial red tide experiment in the East China Sea in 2002. A set of characteristic parameters that describe absorbing crest and reflecting crest of the red tide and its recognition methods are put forward based on general pictre data, with which the spectral information of certain non-dominant alga species of a red tide occurrence is analyzed for establishing the foundation to estimate the species. Comparative experiments have proved that the method is effective. Meanwhile, the transitional area between red-tide zone and non-red-tide zone can be detected with the information of thickness of algae influence, with which a red tide can be forecast.

关键词： red tide recognition aerial remote sensing hyper-spectral image empirical mode decomposition (EMD) characteristic parameter

Metabolism of Mequindox in Isolated Rat Liver Cells

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Journal of Integrative Agriculture 2014年第1期13卷 158-166页

作者： LI Guang-hui SHAN Qi WANG Jing LI Ya-fei GAO Yan ZENG Zhen-ling National Reference Laboratory of Veterinary Drug Residues (SCAU) College of Veterinary MedicineSouth China Agricultural University Jie Yang entry-exit inspection and quarantine bureau

Mequindox （MEQ）, 3-methyl-2-quinoxalinacetyl-l,4-dioxide, is widely used in Chinese veterinary medicine as an antimicrobial agent and feed additive. Its toxicity has been reported to be closely related to its metabolism. To understand the pathways underlying MEQ＇s metabolism more clearly, we studied its metabolism in isolated rat liver cells by using liquid chromatography coupled with electrospray ionization hybrid linear trap quadrupole orbitrap （LC-LTQ-Orbitrap） mass spectrometry. The structures of MEQ metabolites and their product ions were readily and reliably characterized on the basis of accurate MS2 spectra and known structure of MEQ. Eleven metabolites were detected in isolated rat liver cells, two of which were detected for the first time in vitro. The major metabolic pathways reported previously for in vitro metabolism of MEQ in rat microsomes were confirmed in this study, including N O group reduction, carbonyl reduction, and methyl monohydroxylation. In addition, we fotmd that acetyl hydroxylation was an important pathway of MEQ metabolism. The results also demonstrate that cellular systems more closely simulate in vivo conditions than do other in vitro systems such as microsomes. Taken together, these data contribute to our understanding of the in vivo metabolism of MEQ.

关键词： mequindox isolated rat liver cells metabolism metabolites LC-LTQ-Orbitrap

Characterization of Perfluorooctane Sulfonate by Gas Chromatography/Mass Spectrometry

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Journal of Donghua University(English Edition) 2010年第6期27卷 819-824页

作者：孟丽娟杨勇林苗 Key Laboratory of Science & Technology of Eco-Textile Ministry of EducationDonghua University inspection Center of Industrial Product & Raw-Materials Shanghai Entry-Exit Inspection & Quarantine Bureau

With quantitative conversion to volatile derivatives,substance such as perfluorooctane sulfonate(PFOS)that is difficult to analyze can be separated by conventional techniques and then be *** this paper,the methods and procedures in which perfluorooctane sulfonic acids were converted to corresponding sulfonic ester by reaction with triethylorthoacetate(TEOA)in the presence of solvent were described,and then PFOS was analyzed as ethyl ester *** spectra(MS)were generated using electron ionization(EI)*** the EI spectra of the volatile derivatives show ions characteristic of both the fluorinated hydrocarbon and the sulfonic ester portion of the molecules.

关键词： perfluorooctane sulfonate(PFOS) GC/EI-MS characterization derivation

Identification and Characterization of Peptides Mimicking the Epitopes of Metalloprotease of Schistosoma Japonicum

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Cellular & Molecular Immunology 2005年第3期2卷 219-223页

作者： Lianfei Tang Yuxiao Chen Linqian Wang Shunke Zhang Xianfang Zeng Xinyuan Yi Department of Parasitology Xiangya School of Medicine Central SouthUniversity Changsha 410008 China Technology Centre Hunan Entry-Exit Inspection and Quarantine BureauChangsha China

In an attempt to isolate and characterize peptides mimicking epitopes of metalloprotease and explore their immunological protection against Schistosoma japonicum （S. japonicum）, polyclonal anti-metalloprotease sera was prepared to screen a 12-mer random peptide library to isolate phages binding specially to antisera IgG. Then, phage ELISA, animal immunization, DNA sequencing, Western blotting and enzymatic activity neutralizing analysis were used to characterize the selected phage clones. All of ten randomly picked clones were shown to be positive. Five peptides of different amino acid sequences deduced from DNA sequences were obtained and two of them （peptides 2 and 3） could induce significant reduction （31.0% and 31.8%, respectively） in worm burden and high reduction （52.6% and 54.9%, respectively） in liver eggs per gram （LEPG）, while, unexpectedly, others （peptides 1, 4 and 5） could not elicit enough protection against infection of S. japonicum. Peptides 2 and 3 could be recognized by S. japonicum infected mouse sera （IMS） and could elicit neutralizing Abs. The results show that peptides 2 and 3 are antigenic and immunogenic. They are true mimics of epitopes of metalloprotease and useful as novel vaccine candidates against ***. Cellular ＆ Molecular Immunology. 2005;2（3）：219-223.

关键词： phage displayed peptide library epitope Schistosomajaponicum metalloprotease

Determination of Potassium Ferrocyanide in Foods by Resonance Rayleigh Scattering Method with Basic Triaminotriphenylmethane Dyes

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Chinese Chemical Letters 2006年第12期17卷 1595-1598页

作者： Yong Li LI Zhong Fang LIU Li Sha XIE Ling KONG Shao Pu LIU School of Chemistry and Chemical Engineering Southwest University Chongqing 400715 Sichuan entry-exit inspection and quarantine Chengdu 610041

In this work, a sensitive, rapid and simple method for the determination of trace amounts of potassium ferrocyanide in salinized foods and table salt using EV as a RRS probe is established. The detection limit （3σ） of the EV system is 7.8 ng/mL. This new method is more suitable for the determination of the trace amounts of potassium ferrocyanide in colour salinized foods and it can not be disturbed by the color of salinized foods.

关键词： Resonance Rayleigh scattering basic triaminotdphenylmethane dyes potassiumferrocyanide.

Highly sensitive detection of NT-proBNP by molecular motor

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Genes & Diseases 2017年第1期4卷 37-40页

作者： Jie Zhang Yao-Gen Shu Beijing entry-exit inspection and quarantine bureau Beijing100026China Institute of Theoretical Physics Chinese Academy of SciencesBeijing100190China

FoF1-ATPase is an active rotary motor,and generates three-ATP for each *** saturated substrate concentration,the motor can achieve about 103 r.p.m,which means one motor can generate about 105 ATP molecules during 30 ***,we constituted a novel nanodevice with a molecular rotary motor and a“battery”,FoF1-ATPase and chromatophore,and presented a novel method of sandwich type rotary biosensor based on εsubunit with one target-to-one motor,in which one target corresponds 105 ATP molecules as detection signals during 30 *** target such as NT-proBNP detection demonstrated that this novel nanodevice has potential to be developed into an ultrasensitive biosensor to detect low expressed targets.

关键词： Active nanodevice FoF1-ATPase Highly sensitive detection Molecular motor NT-proBNP