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Morphological phylogeny among 15 accessions of bamboos growing in Kokrajhar district of the Bodoland Territorial Area Districts,Assam

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Journal of Forestry research 2018年第5期29卷 1379-1386页

作者： Anusriti Basumatary Sushil Kumar Middha Talambedu Usha Samik Bhattacharya Birendra Kumar Brahma Arvind Kumar Goyal centre for Bamboo Studies and Department of biotechnology Bodoland University DBT-BIF centre Department of BiotechnologyPG Research CentreMaharani Lakshmi Ammanni College for Women Department of Biochemistry Maharani Lakshmi Ammanni College for Women Department of Molecular Ecology Max Planck Institute of Chemical Ecology

Bamboo is nature＇s wonderful gift historically associated with the rural population of the Bodoland Territorial Area ***,indiscriminate use of the resource and the ambiguity in the identification of bamboos in the absence of flowering characters pose a potential risk to the bamboo ***,we considered revising the taxonomic positions and phylogenetic relationships among 15 tropical bamboo species using 35 key morphological descriptors（MD） to assess the reliability of vegetative characters in identifying *** phylogenetic tree constructed from the similarity matrix,derived from an unweighted pair group method of analysis（UpgMA） of the 35 MDs,marginally deviated from the conventional taxonomic positions of the bamboo *** the two varieties,one forma of Bambusa vulgaris（***,***,***） and two species of the genus Dendrocalamus（*** and ***）clustered together in a single clade,and *** was distantly placed from their *** discrepancy with the conventional classification might have ensued since only vegetative characters such as culm anatomy,culm sheath,leaf,and leaf sheath were considered in the present *** the phylogenetic relationship among the 15 tropical bamboos（computed from exhaustive MDs）grossly corroborate the conventional taxonomic grouping,a molecular-marker-based multidisciplinary approach should resolve the remaining conflicts in bamboo identification.

关键词： Bamboo Bodoland Culm Culm-sheath Leaf-sheath Phylogenetic relationship conflict

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Potential of phloroglucinol to improve erectile dysfunction associated with streptozotocin-induced diabetes in rats

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Journal of Integrative Medicine 2019年第4期17卷 282-287页

作者： Syed Kashif Rema Razdan Ramanaiah Illuri Department of Pharmacology Al-Ameen College of Pharmacy Bengaluru Karnataka 560027 India pg and research centre in biotechnology MGR College Hosur Tamil Nadu 635109 India

Objective: Diabetes is a common metabolic disease with several complications in its patients. Often, people living with diabetes develop erectile dysfunction (ED). The primary aim of this work was to investigate the effect of phloroglucinol in diabetes-induced ED in rats. Methods: Male Wistar rats were given 52 mg/kg of streptozotocin, by intraperitoneal injection, to induce diabetes and ED. Subsequently, animals were grouped into three groups: group 1, diabetic control;group 2, low-dose phloroglucinol (150 mg/kg body weight);and group 3, high-dose phloroglucinol (250 mg/kg body weight). A group of six normal rats served as a normal control. The rats were treated with phloroglucinol for six weeks and then were assessed for treatment effects. Sexual behavior, glycosylated hemoglobin Ale (HbAlc) values, serum testosterone, serum nitric oxide (NO), blood pressure and sperm count were measured after the end of treatment. Results: After a 6-week treatment period, the high dose of phloroglucinol significantly decreased HbAlc values in diabetic rats. Rats treated with phloroglucinol had increased serum testosterone, NO and sperm count. Animals treated with 250 mg/kg phloroglucinol performed similar to normal rats in the sexual behavioral study, suggesting the reversal of complications of ED. Conversely, a decrease in the blood pressure in treated groups was observed. Conclusion: The results highlight the protective effect of phloroglucinol in diabetes-induced ED in rats warranting further studies.

关键词： Diabetes Streptozotocin Erectile dysfunction Phloroglucinol Glycated hemoglobin A

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Expression and secretion of Aspergillus niger glucoamylase in Saccharomyces cerevisiae

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Science China Chemistry 1995年第2期38卷 195-201页

作者：李文清何鸣罗进贤 biotechnology research centre Zhongshan University Guagnzhou 510275 China

Aspergillus niger glucoamylase GA 1 cDNA was inserted in between the yeast pgK promoter and terminator on plasmid pMA91. The resultant plasmid pMAG69 was introduced into Saccharomyces cerevisiae GRF18 by protoplast transformation. The A niger GA I cDNA was expressed efficiently under the contiol of pgK promoter and 99% of the gene products were secreted into the culture medium using its own signal sequence The recombmant yeast can digest 87% of starch in 2 d in the medium containing 10% starch. The recombinant plasmid pMAG69 can exist stably in 5. cerevisiae.

关键词： Aspergillus idger glucoamylase gene expression Sacehammyces cerevisiae plasmid stability.

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Genetic variation for phytic acid content in mungbean(Vigna radiata L. Wilczek)

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The Crop Journal 2015年第2期3卷 157-162页

作者： Vinod Janardan Dhole Kandali Srinivasalu Reddy Nuclear Agriculture and biotechnology Division Bhabha Atomic Research Centre

Mungbean(Vigna radiata L. Wilczek) is a short-duration legume crop cultivated for seeds that are rich in protein and carbohydrates. Mungbeans contain phytic acid(PA), an anti-nutritional factor that is the main storage form of organic phosphorus in seeds. It is a strong inhibitor against the absorption of nutrients including iron, zinc, calcium and magnesium in monogastric animals. Genotypes with low phytic acid(lpa) in seed may show increased assimilation of nutrients and be useful in breeding lpa cultivars. The present study was conducted to identify lpa sources, genetic variation, heritability, and association with seed coat color, inorganic phosphorus(IP), and seed size in 102 mungbean genotypes including released varieties, land races, mutants, and wild species grown in two seasons: summer 2011 and rabi 2012. PA and IP in dry seeds were estimated by modified colorimetric method and Chen's modified method,respectively. PA, IP, and 100-seed weight differed significantly in the two seasons. PA content in102 genotypes ranged from 5.74 to 18.98 mg g-1and 5.85 to 20.02 mg g-1in summer 2011 and rabi 2012, respectively. High heritability was found for PA(0.87 and 0.86) and seed size(0.82 and0.83) but low heritability for IP(0.61 and 0.60). A negative correlation was found between PA and seed size(r =-0.183 and-0.267). Yellow and green seed coat genotypes contained significantly less PA than black seed coat genotypes. Cluster analysis revealed the distinctness of wild species, land races and cultivated varieties on the basis of PA content. The genotypes YBSM(6.001 mg g-1) and JL-781(6.179 mg g-1) showed lowest PA. These lpa sources can be used to develop high-yielding mungbean cultivars with low phytic acid.

关键词： Mungbean Phytic acid Inorganic phosphorus Cluster analysis

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Pentachlorophenol degradation by Pseudomonas stutzeri CL7 in the secondary sludge of pulp and paper mill

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Journal of Environmental Sciences 2010年第10期22卷 1608-1612页

作者： Santosh Kr.Karn S.K.Chakrabarty M.S.Reddy Department of biotechnology Thapar University Thapar centre for Industrial research and Development

A pentachlorophenol （PCP） mineralizing bacterium was isolated from the secondary sludge of pulp and paper mill and identified as Pseudomonas stutzeri strain CL7. This isolate used PCP as its sole source of carbon and energy and was capable of degrading this compound as indicated by stoichiometric release of chloride and biomass formation. P. stutzeri （CL7） was able to mineralize a high concentration of PCP （600 mg/L） than any previously reported Pseudomonad with PCP as sole carbon source. As the concentration of PCP increased from 50 to 600 mg/L, the reduction in the cell growth was observed and the PCP degradation was more than 90% in all studied concentrations. This isolate was able to remove 66.8% of PCP from the secondary sludge of pulp and paper mill when supplemented with 100 mg/L of PCP and grown for two weeks. This study showed that the removal efficiency of PCP by CL7 was found to be very effective and can be used in PCP remediation of pulp paper mill waste in the environment.

关键词： pentachlorophenol 16S rRNA secondary sludge bioremediation pulp and paper mill

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Statistical modeling of radiolytic (6OCo γ) degradation of Ofloxacin, antibiotic: Synergetic effect, kinetic studies & assessment of its degraded metabolites

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Frontiers of Environmental Science & Engineering 2019年第3期13卷 147-161页

作者： G. S. Muthu Iswarya B. Nirkayani A. Kavithakani V. C. Padmanaban centre for research Department of Biotechnology Kamaraj College of Engineering & Technology Madurai Tamilnadu India

The impact of human activities in the past few decades has paved the way for the release of pollutants due to the improper effluent treatment. Recent studies revealed that, Ofloxacin, an antibiotic as one of the major pollutant affecting surface water and ground water. In this study, the radiolytic potential of Ofloxacin was investigated. The effects of pH, dose and concentration of Ofloxacin were analyzed using One Factor At a Time (OFAT) and the interactive effects between the parameters were studied using Face Centered Central Composite Design. The statistically optimised developed model shows 30% degradation at initial antibiotic concentration of ImM at pH 3.0 and at 2 kGy dose of gamma ray. The process efficiency was evaluated in terms of G value and its correlation with the concentration of antibiotic was also established. The process of degradation was augmented by the addition of H2O2 (1.5 mM). The reaction kinetics for the process was evaluated, the dose rate constant and the rate of degradation for the augmented process was found to be 0.232 kGy^-1 and 0.232 mM/kGy, respectively. The degraded metabolites of the radiolytic degradation of Ofloxacin were analyzed through change in pH, reduction in TOC and GC-MS spectrum.

关键词： Ofloxacin Gamma irradiation Face centered central composite design Reaction kinetics Gas Chromatography-Mass spectrum

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In vitro antioxidant and cytotoxic activities of essential oil of Feronia elephantum Correa

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Asian Pacific Journal of Tropical Biomedicine 2014年第4期4卷 290-293页

作者： Ramaraj Thirugnanasampandan Delma David pg and research Department of biotechnology Kongunadu Arts and Science College

Objective:To analyse the chemical composition and evaluation of antioxidant,cytotoxic and DN A fragmentation activities of essential oil of Feronia elephantum ***:Chemical composition analysis of hydrodistilled essential oil was determined by gas chromatography-mass spectrometry and in vitro antioxidant activity of oil was determined by DPPH free radical,hydroxyly radical scavenging,metal chelating and prevention of deoxyribose *** and ONA fragmentation activities against breast cancer cells(MCF-7)were also ***:Gas chromatography-mass spectrometry analysis revealed the presence of 24compounds with caryophyllene oxide(62.29%)as major compound.A considerable antioxidant,cyotoxic and DN A fragmentation activities of oils was ***:The result of this study clearly indicates oil could be useful for food preservation and preparation.

关键词： Feronia elephantum Essential oil Antioxidant Cytotoxicity DNA fragmentation

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Genetic variation for seed phosphorus and yield traits in Indian sorghum landraces and varieties

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The Crop Journal 2015年第4期3卷 358-365页

作者： Ashok Badigannavar G.Girish T.R.Ganapathi Nuclear Agriculture and biotechnology Division Bhabha Atomic Research Centre (BARC) Agricultural research Station

Phytic acid is the major storage form of phosphorus in cereals. It binds with nutritionally important metals and affects mineral bioavailability. The present study analyzed phytic acid, inorganic phosphorus(IP) content, seed weight, and grain yield in 98 sorghum landraces and varieties grown in two environments to evaluate genotypic and environmental effects and to determine trait stability. Genotypic effects and genotype × interaction were significant for phytic acid concentration and yield components. A promising landrace, Malkhed-1, had the lowest phytic acid(0.015 mg g-1)concentration, with a higher yield(70.02 g plant-1), than the check variety M-35-1 in both environments. Similarly, among the varieties, Phule Maulee showed the lowest phytic acid(0.07 mg g-1) and a higher grain yield of 53.15 g plant-1in both environments. Phytic acid and IP were negatively correlated(r =- 0.34), whereas grain yield and seed weight were positively correlated(r = 0.20). Cluster analysis based on seed phosphorus traits and yield components identified five and six clusters, respectively. Genotypes containing low phytic acid with high yield identified in this study would be helpful for increasing the bioavailability of mineral nutrients.

关键词： Phytic acid Sorghum Genetic variability Correlation

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Responses of Soil Microbial Activity and Biomass to Salinity After Repeated Additions of Plant Residues

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Pedosphere 2015年第2期25卷 177-185页

作者： Bannur ELMAJDOUB Petra MARSCHNER School of Agriculture Food and WineThe Waite Research InstituteThe University of Adelaide biotechnology research centre

Microbial adaptation to salinity can be achieved through synthesis of organic osmolytes,which requires high amounts of energy;however,a single addition of plant residues can only temporarily improve energy supply to soil ***,a laboratory incubation experiment was conducted to evaluate the responses of soil microbes to increasing salinity with repeated additions of plant residues using a loamy sand soil with an electrical conductivity in saturated paste extract(EC_e) of 0.6 dS m^(-1).The soil was kept non-saline or salinized by adding different amounts of NaCl to achieve EC_e of 12.5,25.0 and 50.0 dS m^(-1).The non-saline soil and the saline soils were amended with finely ground pea residues at two rates equivalent to 3.9 and 7.8 g C kg^(-1) soil on days 0,15 *** soils receiving no residues were included as a *** respiration per g C added over 2 weeks after each residue addition was always greater at 3.9 than 7.8 g C kg^(-1) soil and higher in the non-saline soil than in the saline *** the saline soils,the cumulative respiration per g C added was higher after the second and third additions than after the first addition except with3.9 g C kg^(-1) at EC_e of 50 dS m^(_1).Though with the same amount of C added(7.8 g C kg^(-1)),salinity reduced soil respiration to a lesser extent when 3.9 g C kg^(-1) was added twice compared to a single addition of 7.8 g C kg^(-1).After the third residue addition,the microbial biomass C concentration was significantly lower in the soils with EC_e of 25 and 50 dS m^(_1) than in the non-saline soil at3.9 g C kg^(-1),but only in the soil with EC_e of 50 dS m^(-1) at 7.8 g C kg^(-1).We concluded that repeated residue additions increased the adaptation of soil microbial community to salinity,which was likely due to high C availability providing microbes with the energy needed for synthesis of organic osmolytes.

关键词： C availability electrical conductivity microbial biomass C microbial community respiration saline soil

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STUDIES ON INTRODUCTION OF FOREIGN GENES INTO CULTURED CELLS OF Oryza sativa INDICA USINC Agrobacterium Ti PLASMID SYSTEM

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Science China Chemistry 1991年第1期34卷 54-63页

作者：李宝健欧阳学智许耀 biotechnology research centre Zhongshan University Guangzhou 510275 PRC

Either bacterial attachment or cellulose fibrillar elaboration was hardly observedduring the cocultivation of the cultured suspension cells of Oryza sativa Indica with thestrain C58C1 Rif^r of Agrobacterium tumefaciens that was not specially pretreated. Onthe other hand, quite a lot of Agrobacterium cells were found to adhere to the surface ofcultured rice cells and a number of cellulose fibrils were produced around the specifiedbacteria when phenolics-pretreated bacteria were cocultivated with rice suspension cellsin common culture media, especially in complex culture solutions. The complex culturesolution was the bacterium-free filtrate of hormone-containing MS medium which hadbeen utilized to incubate carrot cells and the newly wounded hypocotyl segments fromtomato and Agrobacterium cells. Detecting experiments demonstrated that both NPT Ⅱ andNOS genes, located on the T-DNA segment of chimaeric plasmid pgV3850 :: 1103neo, weretransferred and expressed in the cultured cells of O. sativa Indica in the optimum trans-formation condition of the present experiment. Integration of the foreign genes into riceDNA was confirmed by Southern blot hybridization.

关键词： rice cell transformation Vir gene activation phenolic compounds interaction of Agrobacterium cells with host cells scanning electron microscope

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