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In vitro larvicidal potential against Anopheles stephensi and antioxidative enzyme activities of Ginkgo biloba,Stevia rebaudiana and Parthenium hysterophorous

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Asian Pacific Journal of Tropical Medicine 2011年第3期4卷 169-175页

作者： Nisar Ahmad Hina Fazal Bilal H Abbasi Mazhar Iqbal Department of Biotechnology Faculty of Biological SciencesQuaid-i-Azam UniversityIslamabad 45320Pakistan Nuclear Institute for Food and Agriculture(NIFA) Peshawar 2500Pakistan Department of Plant Sciences Faculty of Biological SciencesQuaid-i-Azam UniversityIslamabad 45320Pakistan Pakistan Council of Scientific and Industrial Research(pcsir)laboratories Complex Peshawar 2500Pakistan

Objective:To investigate in vitro larvicidal and antioxidant enzymes potential of the medicinal plants Ginkgo biloba（***）,Stevia rebaudiana（***） and Parthenium hysterophorous（***） against Anopheles stephensi（***） 4th instars larvae. Methods:For evaluation of larvicidal potential,the ethanolic,methanolic and dichloromethane leaves extracts of three different plants were used in dose-dependent experiments in two media, while the antioxidant enzymes activities were investigated using four different methods viz., superoxide dismutase,peroxidase,ascorbate and ***:*** has developed resistance to various synthetic insecticides,making its control increasingly *** comparative performance of ethanolic extracts（65%-90%） was found better than the methanolic extract（70%-87%） and dichloromethane extract（60%-70%）.Among the three plants extracts tested in two media,*** exhibited higher larvicidal activity with LC50（24 h） in methanolic extract than *** and *** and *** exhibited the strongest antioxidative enzymes activity and *** were less active and no significant difference was ***:These three plants exhibit larvicidal potential and can be further used for vector control alternative to synthetic insecticide due to eco-friendly and diseases control,furthermore these plant species have potent antioxidative enzyme activities, therefore,making them strong natural candidate particularly for diseases which are caused due to free radicals.

关键词： Larvicidal activity Antioxidative enzymes activities Anopheles stephensi

Estimation and inter-comparison of dust aerosols based on MODIS, MISR and AERONET retrievals over Asian desert regions

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Journal of Environmental Sciences 2019年第2期31卷 154-166页

作者： Ammara Habib Bin Chen Bushra Khalid Saichun Tan Huizheng Che Tariq Mahmood Guangyu Shi Muhammad Tahir Butt Institute of Atmospheric Physics Chinese Academy of Sciences University of Chinese Academy of Sciences Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters Nanjing University of Information Science &Technology International Institute of Applied Systems Analysis Department of Environmental Science International Islamic University Key Laboratory of Atmospheric Chemistry (LAC) Institute of Atmospheric CompositionChinese Academy of Meteorological Sciences (CAMS)CMA Pakistan Meteorological Department Center for Environmental Protection Studies PCSIR Laboratories Complex

This study presents detailed analysis of spatiotemporal variations and trend of dust optical properties i.e., Aerosol Optical Depth(AOD) and Angstrom component over Asian desert regions using thirteen years of data(i.e., 2001–2013) retrieved from Aerosol Robotic Network(AERONET), Moderate Resolution Imaging Spectroradiometer(MODIS) and Multi-angle Imaging Spectroradiometer(MISR). These regions include Solar Village, Dunhuang and Dalangzadgad and are considered as origin of desert aerosols in Asia. Mann–Kendall trend test was used to show the trend of AOD. The relationship of AOD with weather parameters and general AOD trend over different wavelengths has also been shown. AOD's trend has been observed significant throughout the year in Solar Village, while in Dunhuang and Dalanzadgad the significant trend has been found only in peak period(March–June).Analysis show high values of AOD and low values of angstrom in Solar Village during peak period. In Chinese desert regions, high values of AOD have been found during peak period and low values in pre-peak period. Significant relationship has been observed between AOD and average temperature in Solar Village and Dalanzadgad whereas rainfall and wind speed showed no significant impact on AOD in all desert regions.

关键词： AOD Angstrom exponent AERONET MODIS MISR Desert

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Microwaves spark emission spectroscopy for the analysis of cations:A simple form of atomic emission spectroscopy

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Chinese Chemical Letters 2011年第9期22卷 1084-1086页

作者： Zahid Hussain Khalid Mohammed Khan Khadim Hussain Sadam Hussain Shahnaz Perveen Department of Chemistry Abdul Wali Khan UniversityMardanPakistan H.E.J.Research Institute of Chemistry International Center for Chemical and Biological SciencesUniversity of KarachiKarachi 75270Pakistan Department of Chemistry Hazara UniversityMansehraPakistan Department of Electronics Quaid-I-Azam UniversityIslamabadPakistan pcsir laboratories Complex Shahrah-e-Dr.Salimuzzaman SiddiquiKarachi 75280Pakistan

A novel method for the cation analysis was investigated. The analysis is based on the sparking of the salts of metals in a microwave oven after placing in a graphite cell. The graphite cell absorbs microwaves and produces high temperature which converts the salt into light emitting species. The colour of light was found to dependent on the nature of cation, however, the intensity of the emitted light was found to be depending upon the form and shape of the graphite assembly in addition to the concentration of the salt. This communication presents explanation for all these observations and for the systematic and quantitative analysis using microwave spark emission technique.

关键词： Microwave spark Cation analysis Spark colour Graphite cell Atomic emission

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Synthesis,Crystal Structure and Spectroscopic Properties of 1,2-Benzothiazine Derivatives:An Experimental and DFT Study

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Chinese Journal of Structural Chemistry 2015年第1期34卷 15-25页

作者： MUHAMMAD Nadeem Arshad TARIQ Mahmood ATHER Faroque Khan MUHAMMAD Zia-Ur-Rehman ABDULLAH M.Asiri ISLAM Ullah Khan RIFFAT-Un-Nisa KHURSHID Ayub AZAM Mukhtar MUHAMMAD Tariq Saeed Chemistry Department Faculty of ScienceKing Abdulaziz University Centre of Excellence for Advanced Materials Research (CEAMR) King Abdulaziz University Department of Chemistry COMSATS Institute of Information TechnologyAbbottabad IRCBM COMSATS Institute of Information Technology Applied Chemistry Research Center PCSIR Laboratories ComplexFerozpur RoadLahore 54600Pakistan Materials Chemistry Laboratory Department of ChemistryGC University Department of Chemistry College of ScienceKing Faisal University Department of Chemistry University of EducationOkara CampusOkara-Pakistan

1,2-Benzothiazine derivatives methyl 3-methoxy-4-oxo-3,4-dihydro-2H-benzo[e] [1,2]thiazine-3-carboxylate 1,1-dioxide（1） and methyl 2-ethyl-3-hydroxy-4-oxo-3,4-dihydro-2Hbenzo[e][1,2]thiazine-3-carboxylate 1,1-dioxide（2） were synthesized, and characterized by spectroscopic techniques; 1H-NMR and infrared（IR） spectroscopy. Crystals of 1 and 2 were grown by slow evaporation of methanol and ethyl acetate, respectively and their crystal structures were investigated by single-crystal X-ray diffraction analysis. Geometric properties were calculated by the B3 LYP method of density functional theory（DFT） at the 6-31G＋（d） basis set to compare with the experimental data. Simulated properties were found in strong agreement with the experimental ones. Intermolecular forces have also been modeled in order to investigate the strength of packing and strong hydrogen bonding was observed in both compounds 1 and 2. Electronic properties such as Ionization Potential（IP）, Electron Affinities（EA） and coefficients of the highest occupied molecular orbital（HOMO） and the lowest unoccupied molecular orbital（LUMO） of com- pounds 1 and 2 were simulated for the first time.

关键词： 1,2-benzothiazine B3LYP/6-31+G (d) density functional theory (DFT) HUMO-LUMO crystal structures

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菲律宾蛤仔过敏原可视化抗体微阵列玻片的检测方法

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水产学报 2010年第3期34卷 422-427页

作者：李小燕李振兴林洪 SAMEE Haider 张轶群中国海洋大学水产品安全性实验室 Food and Marine Research Center PCSIR Laboratories ComplexOff University Road

采用辣根过氧化物酶(HRP)与3,3′,5,5′—四甲基联苯胺(TMB)—H2O2作为信号示踪系统,建立了一种可视化抗体微阵列检测菲律宾蛤仔过敏原的方法。对孵育时间、抗体浓度等免疫条件进行了优化。采用改良双抗体夹心法,将菲律宾蛤仔过敏原的... 详细信息

采用辣根过氧化物酶(HRP)与3,3′,5,5′—四甲基联苯胺(TMB)—H2O2作为信号示踪系统,建立了一种可视化抗体微阵列检测菲律宾蛤仔过敏原的方法。对孵育时间、抗体浓度等免疫条件进行了优化。采用改良双抗体夹心法,将菲律宾蛤仔过敏原的兔多克隆抗体固定于琼脂糖三维芯片上,依次加入待检样品、菲律宾蛤仔鼠多克隆抗体和HRP-羊抗鼠IgG,孵育后加TMB显色,肉眼观察后,用平板扫描仪获取扫描图象,采用GenePixPro6.0软件分析灰度值。试验结果表明,该方法最低可检出10ng/mL的菲律宾蛤仔过敏原,片内平均变异系数(CV)为5.99%,片间为10.3%;香肠及蟹棒中3个不同浓度的加标回收率为73.54%～95.44%;4℃存放4个月内抗体微阵列玻片活性保持稳定。该方法不需要大型精密仪器,结果直观可见,可以发展为对多种过敏原进行同时检测,具有良好的实用和推广价值。

关键词：菲律宾蛤仔过敏原夹心法抗体微阵列可视化

Dengue fever treatment with Carica papaya leaves extracts

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Asian Pacific Journal of Tropical Biomedicine 2011年第4期1卷 330-333页

作者： Nisar Ahmad Hina Fazal Muhammad Ayaz Bilal Haider Abbasi Ijaz Mohammad Lubna Fazal Faculty of Biological Sciences Quaid-i-Azam UniversityIslamabad 45320Pakistan Nuclear Institute for Food and Agriculture(NIFA) Tarnab Peshawar 25000Pakistan Pakistan Council of Scientific and Industrial Research(pcsir) laboratories Complex Peshawar 25000Pakistan Department of Environmental Sciences University of PeshawarPeshawar 25000Pakistan Postgraduate Medical Institute(PGMI) Lady Reading HospitalPeshawar 25000Pakistan

The main objective of the current study is to investigate the potential of Carica papaya leaves extracts against Dengue fever in 45 year old patient bitten by carrier *** the treatment of Dengue fever the extract was prepared in water.25 mL of aqueous extract of *** leaves was administered to patient infected with Dengue fever twice daily *** and evening for five consecutive *** the extract administration the blood samples from patient were *** count(PLT),White Blood Cells(WBC) and Neutrophils(NEUT) decreased from 176×10~3/μ L,8.10×10~3/μ L,84.0%to 55×10~3/μ L,3.7×10~3/μL and 46.0%.Subsequently,the blood samples were rechecked after the administration of leaves *** was observed that the PLT count increased from 55×10~3/μ L to 168×10~3/μ L,WBC from 3.7×10~3/μ L to 7.7×10~3/μ L and NEUT from 46.0%to 78.3%.From the patient feelings and blood reports it showed that Carica papaya leaves aqueous extract exhibited potential activity against Dengue ***,the different parts of this valuable specie can be further used as a strong natural candidate against viral diseases.

关键词： Dengue fever Carica papaya Leaves extracts Platelets Potential activity