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2020-2022年湖南省326824名宫颈癌筛查和健康体检者HPV感染率及亚型分布

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中华肿瘤防治杂志 2024年第7期31卷 399-404页

作者：陈少强吴颖谢艳郭俊龙李菁谢小兵李萍湖南中医药大学第一附属医院医学检验与病理中心湖南长沙410007 长沙迪安医学检验所有限公司pcr室湖南长沙410007

目的分析湖南省女性人乳头瘤病毒(HPV)感染情况以及HPV亚型与宫颈上皮细胞和组织病理改变的关系,为当地HPV流行病学提供数据支持。方法采集2020-2022年湖南省326824名参加宫颈癌筛查和健康体检人群的宫颈脱落上皮细胞,采用荧光pcr技术... 详细信息

目的分析湖南省女性人乳头瘤病毒(HPV)感染情况以及HPV亚型与宫颈上皮细胞和组织病理改变的关系,为当地HPV流行病学提供数据支持。方法采集2020-2022年湖南省326824名参加宫颈癌筛查和健康体检人群的宫颈脱落上皮细胞,采用荧光pcr技术检测其HPV分型,统计分析不同年龄、地区各HPV分型感染状况;其中9940例进行液基薄层细胞学检查(TCT)检查或病理学检查,分析其HPV感染情况与细胞病理学阳性结局的关系。结果HPV总体感染率为10.9%(35646/326824),其中单一型感染占81.9%(29201/35646),多重型感染占18.1%(6445/35646);单一型占比前6位型别依次为HPV52(20.5%)、HPV58(16.0%)、HPV16(12.9%)、HPV53(11.9%)、HPV81(8.7%)和HPV70(8.6%)。≥60岁组感染率最高,为15.7%(3672/23315),HPV感染阳性人群中,不同年龄组多重型感染占比总体差异有统计学意义,χ^(2)=365.910,P<0.001。按病例来源划分,感染率最高的地区为张家界市〔13.1%(2089/15982)〕,最低的地区为永州市〔8.7%(3219/37058)〕。单一型感染者组织和细胞学阳性率为47.1%(3618/7683),多重型感染者阳性率为58.8%(1326/2257),χ^(2)=94.867,P<0.001。在7683例单一型HPV感染者中,HPV39、HPV66、HPV16、HPV6、HPV33、HPV42、HPV59和HPV58组织和细胞学阳性率均超过50%。结论≥60岁人群HPV感染率最高,应加强对这一人群HPV感染的防治力度;HPV52和HPV58是全部人群中最为常见的型别;多重型HPV感染更可能导致宫颈疾病的发生。

关键词：人乳头瘤病毒感染率宫颈肿瘤宫颈上皮内瘤样病变湖南省

Evaluation of immunogenicity and reactogenicity of recombinant DNA hepatitis B vaccine produced in India

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World Journal of Gastroenterology 2005年第45期11卷 7165-7168页

作者： Zahid Hussain Syed S Ali Syed A Husain Mohammad Raish Deepika R Sharma Premashis Kar pcr hepatitis laboratory Panacea Biotec Ltd Mohammad Raish

AIM：（1） To gain information on immune responses to an accelerated schedule of 0, 1, and 2 mo in paramedical staff and BDS students who are at an increased risk of getting hepatitis B infection and come under high risk groups. （2） To assess the efficacy and safety of En/vac- HB in different age groups, using genetically modified yeast strain Pichia pastoris, a new recombinant hepatitis B accine developed and manufactured in India. METHODS： A prospective, comparative, and single blinded trial of rapid （0, 1, and 2 mo） hepatitis B immunization schedulewas reported. A total of three hundred and seven （212 females and 95 males） healthy volunteers divided into three age groups （18-29, 30-39, and 40-49） were enrolled after screening for markers of hepatitis B. All the volunteers received 20 mg of the vaccine intramuscularly at 0, 1, and 2 mo. RESULTS： Geometric mean titers were calculated pre and post vaccination. Before immunization the GMT was 0.0124 mIU/mL. One month after the administration of the third dose of recombinant vaccine 296/307 （96.5%） subjects achieved seroprotective levels of anti-HBs. The geometric mean anti-HBs titers achieved after one month of the third dose was 2 560.0 mIU/mL. The geometric mean anti-HBs titer of males was 2 029.0 mIU/mL, while that of the females was 2 759.0 mIU/mL. In the age group of 18-29 years, anti-HBs titer was 3 025.0 mIU/ mL, while that in the age group of 30-39 years was 2 096.0 mlU/mL. In third age group of 40-49 years, anti- HBs titer was 1 592.0 mIU/mL. Hyper-responses （anti-HBs≥100 mIU/mL） were shown in 88.0% （271/307） of subjects. Eleven （3.5%） subjects responded poorly to the vaccine in the age group of 40-49 years. There was only mild pain at the site of injection otherwise there were no other adverse drug reactions （ADRs）. CONCLUSION： This vaccine （Enivac-HB） is safe and efficacious, providing significant protection after the third dose and rapid hepatitis B immunization schedule of 0, 1, and 2 mo can be recommended

关键词： Enivac-HB Pichia pastoris Anti-HBsantibody hepatitis B vaccine

Virological course of hepatitis A virus as determined by real time RT-pcr: Correlation with biochemical, immunological and genotypic profiles

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World Journal of Gastroenterology 2006年第29期12卷 4683-4688页

作者： Zahid Hussain Bhudev C Das Syed A Husain Sunil K Polipalli Tanzeel Ahmed Nargis Begum Subhash Medhi Alice Verghese Mohammad Raish Apiradee Theamboonlers Yong Poovorawan Premashis Kar pcr hepatitis laboratory Department of Medicine Maulana Azad Medical CollegeNew Delhi 110002India Human Genetics LaboratoryDepartment of BiosciencesJamia Millia IslamiaNew Delhi 110025India Division of Molecular Oncology Institute of Cytology and Preventive Oncology (ICMR)NoidaSector 39Uttar PradeshIndia Human Genetics laboratory Department of Biosciences Jamia Millia IslamiaNew Delhi 110025India pcr hepatitis laboratory Department of Medicine Maulana Azad Medical CollegeNew Delhi 110002India Advance Center for AIDS National Institute of Communicable Diseases New Delhi 110041India Viral hepatitis Research Unit Department of Pediatric Faculty of MedicineChulalolongkorn University and HospitalRama Ⅳ RoadBangkok 10330Thailand pcr hepatitis laboratory Department of Medicine Maulana Azad Medical College New Delhi 110002India

AIM： To undertake analysis of hepatitis A viral load, alanine aminotransferase （ALT）, and viral genotypes with duration of viremia, and to correlate these parameters with CD4^＋/ CD8^＋ lymphocyte populations that control cell-mediated immunity. METHODS： Cell counts were carried out using fresh whole blood collected in EDTA vials using a fluorescence activated cell sorter. hepatitis A virus （HAV） RNA was extracted from blood serum, reverse transcribed into cDNA and quantified by Real-Time polymerase chain reaction and was genotyped. RESULTS： Among 11 patients, 10 could be analyzed completely. Of these, 3 had severe acute hepatitis （s-AH） and the remainder had a self-limited acute hepatitis A （AHA）, with one patient with fulminant disease （encephalopathy Grade IV） dying on the 4^th d. The ALT level was significantly higher both in AHA （1070.9±894.3; P = 0.0014） and s-AH （1713.9±886.3; P = 0.001） compared to normal controls （23.6±7.2）. The prothrombin time in s-AH patients （21.0 ±2.0; P=0.02） was significantly higher than in AHA （14.3±1.1;P = 0.44）. The CD4^＋/CD8^＋ ratio in AHA patients （1.17 ＋ 0.11; P = 0.22） and s-AH （0.83 ＋ 0.12; P = 0.0002） were lower than seen in normal healthy controls （1.52）. Self-limited cases had peak viral load at the beginning of analysis while in s-AH patients this occurred at the 15TM or 30^th d. In acute and severe groups, one patient each belonged to genotype IA, with the remaining 8 cases belonging to genotype IIIA. The only fulminant hepatic failure case belonged to genotype IA. HAV viral load and AIT values collected during the entire course of the selflimited infection were directly correlated but this was not the case for s-AH patients.CONCLUSION： Based on a small-scale study, the persistently higher viral load of s-AH might be due to diminished cellular immunity and hemolysis. The duration of viremia was dependent on the host, as the viral genotype had no apparent role in clinical outcome of AVH and s-AH cases.

关键词： Viral load Real-time pcr Immunologicalresponse Severe acute hepatitis Self-limited acute henatitis

CALCULATION OF HIGH-PRESSURE VAPORLIQUID EQUILIBRIA

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Science China Mathematics 1992年第9期35卷 1126-1135页

作者：罗瑞贤陈亮环陈霭璠 Department of Applied Chemistry Beijing Institute of Chemical Technology Beijing 100029 PCR

Using the principle of corresponding states (PCS) of molecular shape factors, the highpressure vapor-liquid equilibrium for a binary system of light gases with hydrocarbons is calculated. The van der Waals one-fluid mixture model, with two binary interaction parameters, is used to determine the conformal parameters needed to describe mixture behavior, and the Marquardt optimization nonlinear program is used to determine optimum values of parameters. The calculated equilibrium ratios by specifying temperatures as functions of pressures agree well with experimental data. Only two conformal parameters are needed in the shape factor method for calculating mixtures.

关键词： equilibrium ratio principle of corresponding states shape factor.

乙丙型肝炎病毒双重感染外周血淋巴细胞亚群表达

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中华医院感染学杂志 2024年第14期34卷 2099-2102页

作者：刘伶王栋贺松赵偲韩凌飞安徽省马鞍山市临床检验中心pcr实验室安徽马鞍山243000 江苏省南京医科大学第四附属医院检验科江苏南京210031

目的探讨乙丙型肝炎病毒双重感染外周血淋巴细胞亚群表达.方法选取2022年10月-2023年3月于安徽省马鞍山市传染病医院收治的67例慢性病毒性肝炎患者为研究对象.根据感染病毒类型分为慢性乙型肝炎组30例、慢性丙型肝炎组25例与双重感染组1... 详细信息

目的探讨乙丙型肝炎病毒双重感染外周血淋巴细胞亚群表达.方法选取2022年10月-2023年3月于安徽省马鞍山市传染病医院收治的67例慢性病毒性肝炎患者为研究对象.根据感染病毒类型分为慢性乙型肝炎组30例、慢性丙型肝炎组25例与双重感染组12例,另选取同期30名健康体检者为对照组.采用流式细胞技术检测各组外周血淋巴细胞亚群:总T淋巴细胞数(CD_(3)^(+))、总B淋巴细胞数(CD_(19)^(+))、辅助性T淋巴细胞数(CD_(4)^(+))、细胞毒性T淋巴细胞数(CD_(8)^(+))、NK淋巴细胞数(CD_(3)^(-)CD_(16)^(+)CD5_(6)^(+)),及CD_(4)^(+)/CD_(8)^(+)比值,分析各组之间相关性.结果不同组别的年龄与性别比较,无统计学差异;双重感染组CD_(3)^(+)、CD_(19)^(+)、CD_(4)^(+)、CD_(8)^(+)、NK细胞、CD_(4)^(+)/CD_(8)^(+)水平低于健康对照组和慢性丙型肝炎组(P<0.05),与慢性乙型肝炎组比较,无统计学差异.慢性乙型肝炎组CD_(3)^(+)、CD_(4)^(+)、NK细胞水平低于健康对照组(P<0.05);慢性乙型肝炎组CD_(3)^(+)、CD_(4)^(+)、CD_(19)^(+)、CD_(8)^(+)水平低于慢性丙型肝炎组(P<0.05);慢性丙型肝炎组NK细胞水平低于健康对照组(P<0.05).结论外周血淋巴细胞亚群检测可以作为评估乙丙型肝炎病毒双重感染、乙型肝炎及丙型肝炎的免疫水平指标,对患者的免疫治疗和预后判断具有重要意义.

关键词：乙丙型肝炎病毒双重感染慢性乙型肝炎慢性丙型肝炎淋巴细胞亚群免疫水平

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Viral Genotypes and Associated Risk Factors of Hepatocellular Carcinoma in India

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Clinical oncology and cancer researeh 2012年第3期9卷 172-181页

作者： Manash Pratim Sarma Mohammad Asim Subhash Medhi Thayumanavan Bharathi Richa Diwan Premashis Kar pcr hepatitis laboratory Department of MedicineMaulana Azad Medical CollegeUniversity of Delhi Department of Gastroenterology Rajaji Government Hospital

Objective This study aims to investigate the etiological relationship among hepatitis B virus （HBV）, hepatitis C virus （HCV）, and alcohol as risk factors in a cohort of hepatocellular carcinoma （HCC） patients from India. The clinical and biochemical profiles and tumor characteristics in the HCC cases were also evaluated. Methods A total of 357 consecutive cases of HCC fulfilling the diagnostic criteria from the Barcelona-2000 EASL conference were included in the study. The blood samples were evaluated for serological evidence of HBV and HCV infection, viral load, and genotypes using serological tests, reverse transcription-polymerase chain reaction, and restriction fragment length polymorphism. Results The male/female ratio for the HCC cases was 5.87：1. Majority of the HCC patients （33.9%） were 50 to 59 years of age, with a mean age of 4±13.23 years. More than half the cases （60.8%） had underlying cirrhosis at presentation. Among the HCC patients, 68.9% were HBV related, 21.3% were HCV related, 18.8%, were alcoholic, and 18.2% were of cryptogenic origin. The presence of any marker positive for HBV increased the risk for developing HCC by almost 27 times [OR： 27.33; （12.87-60.0）]. An increased risk of 10.6 times was observed for HCC development for cases positive for ally HCV marker [OR： 10.55; （3.13-42.73）]. Heavy alcohol consumption along with HCV RNA positivity in cirrhotic patients was found to be a risk for developing HCC by 3 folds ]OR： 3.17; （0.37-70.71）]. Conclusions Patients of chronic HBV infection followed by chronic HCV infection were at higher risk of developing HCC in India. Chronic alcohol consumption was found to be a risk factor in cirrhotic cases only when it was associated with HCV RNA positivity. Most of the patients had a large tumor size （〉5 cm） with multiple liver nodules, indicating an advanced stage of the disease thus making curative therapies difficult.

关键词： hepatocellular carcinoma hepatitis B virus hepatitis C virus risk factors

A Binomial Model Approach: Comparing the R0 Values of SARS-CoV-2 rRT-pcr Data from laboratories across Northern Cyprus

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Computer Modeling in Engineering & Sciences 2021年第8期128卷 717-729页

作者： Nazife Sultanoglu Nezihal Gokbulut Tamer Sanlidag Evren Hincal Bilgen Kaymakamzade Murat Sayan Department of Medical Microbiology and Clinical Microbiology Faculty of MedicineNear East UniversityNicosia99138Cyprus DESAM Research Institute Near East UniversityNicosia99138Cyprus Department of Mathematics Near East UniversityNicosia99138Cyprus Faculty of Medicine Kocaeli UniversityClinical LaboratoryPCR UnitKocaeli41001Turkey

Northern Cyprus has implemented relatively strict measures in the battle against the outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The measures were introduced at the beginning of the COVID-19pandemic, in order to prevent the spread of the disease. One of these measures was the use of two separate realtime reverse transcription polymerase chain reaction (rRT-pcr) tests for SARS-CoV-2 referred to as the doublescreening procedure, which was adopted following the re-opening of the sea, air and land borders for passengersafter the first lockdown. The rRT-pcr double screening procedure involved reporting a negative rRT-pcr testwhich was carried in 72 to 120 h before departure whilst presenting no known symptoms of the COVID-19and performing a second rRT-pcr test at the point of arrival. This study compares the results of SARS-CoV-2rRT-pcr tests performed on incoming flight passengers from the 1st July to 9th of September 2020 to NorthernCyprus. The rRT-pcr test results collected by the Near East University (NEU) DESAM COVID-19 laboratory werecompared with the rRT-pcr test results collected by the Ministry of Health and/or private COVID-19 laboratoriesin Northern Cyprus. This comparative study was conducted using binomial distribution. In addition, by applyingthe Susceptible-Exposed-Infected-Removed (SEIR) model to Northern Cyprus, overall basic reproduction number(R0) value of the COVID-19 was analysed for the same time period to act as a threshold for this *** both the statistical and SEIR mathematical model, R0 was calculated. It was assumed that, the more similarthe R0 results of NEU DESAM COVID-19 laboratory and other laboratories were with the overall R0 value ofNorthern Cyprus, the more reliable the results would be. We calculated that the median R0 values of the NEUDESAM COVID-19 laboratory and other laboratories performing the SARS-CoV-2 rRT-pcr on air passengersduring the studied period to be 0.96 and 1.29, respectively, com

关键词： COVID-19 tests performed basic reproduction number statistical analysis comparison

荧光定量pcr技术在产前诊断中的应用专家共识

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中华妇产科杂志 2016年第5期51卷 321-324页

作者：荧光定量pcr技术在产前诊断中的应用协作组荧光定量pcr技术在产前诊断中的应用协作组

自2002年起，我国启动了以唐氏综合征等常见染色体异常为主要目标疾病的产前筛查及产前诊断，染色体核型分析技术是确诊的“金标准”。染色体核型分析技术准确、可靠，但以手工操作为主，需要进行细胞培养，存在耗时长、检测通量低、需... 详细信息

自2002年起，我国启动了以唐氏综合征等常见染色体异常为主要目标疾病的产前筛查及产前诊断，染色体核型分析技术是确诊的“金标准”。染色体核型分析技术准确、可靠，但以手工操作为主，需要进行细胞培养，存在耗时长、检测通量低、需培养专业人员及实验室建设周期长等诸多问题，已难以满足日益增长的临床产前诊断需求。

关键词：荧光定量pcr技术产前诊断染色体核型分析专家应用细胞培养染色体异常唐氏综合征

Serum levels of mi RNA in patients with hepatitis B virus-associated acute-on-chronic liver failure

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Hepatobiliary & Pancreatic Diseases International 2018年第2期17卷 126-132页

作者： Ya Wen Shi-Fang Peng Lei Fu Xiao-Yu Fu Da-Xian Wu Bing-Jie Liu De-Ming Tan Yi Ouyang Key laboratory of Hunan Viral hepatitis Department of Infectious Disease Xiangya Hospital Central South University

Background: hepatitis B virus(HBV)-associated acute-on-chronic liver failure(HBV-ACLF) is a lifethreatening condition and its exact pathophysiology and progression remain unclear. The present study aimed to assess the role of serum mi RNAs in the evaluation of HBV-ACLF and to develop a model to predict the outcomes for ***: Serum was collected from 41 chronic hepatitis B and 55 HBV-ACLF patients in addition to30 chronic asymptomatic HBV carriers as controls. The mi RNAs expressions were measured by real-time quantitative pcr(q-pcr). Statistical analyses were conducted to assess the ability of differentially expressed mi RNAs and other prognostic factors in identifying ACLF prognosis and to develop a new predictive ***: Real-time q-pcr indicated that serum miR-146 a-5 p, mi R-122-3 p and mi R-328-3 p levels were significantly upregulated in ACLF patients compared to chronic hepatitis B and chronic asymptomatic HBV carriers patients. In addition, multivariate regression analyses indicated that Na+, INR, gastrointestinal bleeding and mi R-122-3 p are all independent factors that are reliable and sensitive to the prognosis of HBV-ACLF. Therefore, we developed a new model for the prediction of HBV-ACLF disease state: Y = 0.402 × Na+-1.72 × INR-4.963 × gastrointestinal bleeding(Yes = 0; No = 1)-0.278 ×(mi R-122-3 p) + 50.449. The predictive accuracy of the model was 95.3% and the area under the receiver operating characteristic curve(AUROC) was ***: Expression levels of these mi RNAs(miR-146 a-5 p, mi R-122-3 p and mi R-328-3 p) positively correlate with the severity of liver inflammation in patients with ACLF and may be useful to predict HBV-ACLF severity.

关键词： MicroRNA hepatitis B virus Acute-on-chronic liver failure Prognosis