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A photoactivatable and phenylboronic acid-functionalized nanoassembly for combating multidrug-resistant gram-negative bacteria and their biofilms

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Burns & Trauma 2023年第1期11卷 765-791页

作者： Xiaoqing Zhou Lanlan Dong Baohua Zhao Guangyun Hu Can Huang Tengfei Liu Yifei Lu Mengxue Zheng Yanlan Yu Zengjun Yang Shaowen Cheng Yan Xiong Gaoxing Luo Wei Qian Rui Yin department of Dermatology Southwest HospitalArmy Medical University(Third Military Medical University)No.29 Gaotanyan RoadShapingba DistrictChongqing 400038China institute of Burn research State Key Laboratory of TraumaBurn and Combined InjuryKey Laboratory of Disease Proteomics of ChongqingSouthwest HospitalArmy Medical University(Third Military Medical University)No.29 Gaotanyan RoadShapingba DistrictChongqing 400038China department of Burn and Plastic Sugery No.906 Hospital of Joint Logistic Support Force of PLANo.377 Zhongshan East RoadYinzhou DistrictNingbo 315100China department of Wound Repair the First Affiliated Hospital of Hainan Medical UniversityNo.31 Longhua RoadHaikou 570102China department of orthopaedics Daping HospitalArmy Medical University(Third Military Medical University)No.10 Changjiang Branch RoadYuzhong DistrictChongqing 400042China

Background:Multidrug-resistant(MDR)gram-negative bacteria-related infectious diseases have caused an increase in the public health burden and ***,the formation of biofilms makes these bacteria difficult to ***,developing novel interventions to combat MDR gram-negative bacteria and their biofilms-related infections are urgently *** purpose of this study was to develop a multifunctional nanoassembly(IRNB)based on IR-780 and N,N-di-sec-butyl-N,N-dinitroso-1,4-phenylenediamine(BNN6)for synergistic effect on the infected wounds and subcutaneous abscesses caused by gram-negative ***:The characterization and bacteria-targeting ability of IRNB were *** bac-tericidal efficacy of IRNB against gram-negative bacteria and their biofilms was demonstrated by crystal violet staining assay,plate counting method and live/dead staining in *** antibacterial efficiency of IRNB was examined on a subcutaneous abscess and cutaneous infected wound model in vivo.A cell counting kit-8 assay,Calcein/PI cytotoxicity assay,hemolysis assay and intravenous injection assay were performed to detect the biocompatibility of IRNB in vitro and in ***:Herein,we successfully developed a multifunctional nanoassembly IRNB based on IR-780 and BNN6 for synergistic photothermal therapy(PTT),photodynamic therapy(PDT)and nitric oxide(NO)effect triggered by an 808 nm *** nanoassembly could accumulate specifically at the infected sites of MDR gram-negative bacteria and their biofilms via the covalent coupling *** irradiation with an 808 nm laser,IRNB was activated and produced both reactive oxygen species(ROS)and *** local hyperthermia could induce NO generation,which further reacted with ROS to generate ONOO−,leading to the enhancement of bactericidal ***,NO and ONOO−could disrupt the cell membrane,which converts bacteria to an extremely susceptible state and further enhances the phototherma

关键词： Photothermal therapy Photodynamic therapy Nitric oxide Synergistic Boronic acid Multidrug-resistant gram-negative bacteria

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The Chondrogenic Potential of Rabbit Bone-marrow-derived Mesenchymal Stem Cells (MSCs) Assayed under the Influence of Growth Factors

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The Journal of Biomedical research 2002年第3期16卷 121-126页

作者：尹战海刘淼王金堂张璟曹峻岭郑钧 department of orthopaedics the First Hospital of Xi'an Jiaotong Univer-sity Xian 710061 department of Hemotology the First Hospital of Xi'an Jiaotong Univer-sity Xian 710061 institute of Endemic Diseases Medical College of Xi'an Jiaotong UniversityXi'an 710061 P. R. China

Objective To establish a method of inducing rabbit bone marrow mesenchymal stem cells (MSCs) to express chondrocytic phenotype. Methods MSCs were seperated from bone marrow extracted from the iliac of New Zealand rabbit. TGF β 1, IGF Ⅰ and Vitamin C were applied into culture medium to induce proliferation and transformation of MSCs. Colony forming efficiency(CFE) was measured to reflect cell proliferation rate; procollagen α1(Ⅱ)mRNA in cells was detected by RT PCR; ultrastructure of cells was observed under scanning electronic microscope(SEM);alkline phosphatase(ALP) in culture medium was also detected. Results Under the influence of TGF β 1, IGF Ⅰ and Vitamine C, CFE of MSCs rose from control level of 3/106 to 21/106, expression of articular cartilage specific procollagen α1(Ⅱ)mRNA by cells was promoted; At the same time, flocculent matrixes synthesized were observed between connecting polygonal MSCs. ALP in culture medium declined to control level with culture time. Conclusion Expression of chondrocytic phenotype by MSCs could be induced by the synergistic action of TGF β 1,IGF Ⅰ and Vitamine C. Growth factor induced MSCs could be a good cell source for tissue engineered cartilage.

关键词： mesenchymal stem cells cell culture cartilage

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Influence of microstructural characteristics on corrosion behavior of Mg-5Sn-3In alloy in Hank’s solution

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Transactions of Nonferrous Metals Society of China 2021年第10期31卷 2999-3011页

作者： Xue-jian WANG Zong-ning CHEN Yu-bo ZHANG En-yu GUO Hui-jun KANG Pei HAN Tong-min WANG Key Laboratory of Solidification Control and Digital Preparation Technology(Liaoning Province) School of Materials Science and EngineeringDalian University of TechnologyDalian 116024China Ningbo institute of Dalian University of Technology Ningbo 315000China orthopaedic department Shanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghai 200233China

The microstructural observation,the mass loss test,potentiodynamic polarization measurements and corrosion morphology examinations were conducted to study the influence of microstructural characteristics on corrosion behavior of Mg–5Sn–3In alloys in Hank’s solution after *** results show that the corrosion rate of the as-cast alloy is similar to that of as-extruded alloy;however,the local corrosion susceptibility is greatly weakened in the as-extruded alloy,especially in the extrusion *** relatively uniform corrosion morphology of the as-extruded alloy is attributed to refined Mg_(2)Sn particles,uniform distribution of Mg_(2)Sn particles and favorable crystal ***,the cytotoxicity tests confirm that the Mg–5Sn–3In alloy exhibits cytotoxicity of Grade 0−1 for NIH3T3 cells,suggesting an acceptable cytotoxicity of this alloy in the vitro assay.

关键词： Mg–Sn alloy indium element microstructure corrosion behavior cytotoxicity

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Combining single-cell RNA sequencing and population-based studies reveals hand osteoarthritis-associated chondrocyte subpopulations and pathways

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Bone research 2023年第4期11卷 834-846页

作者： Hui Li Xiaofeng Jiang Yongbing Xiao Yuqing Zhang Weiya Zhang Michael Doherty Jacquelyn Nestor Changjun Li Jing Ye Tingting Sha Houchen Lyu Jie Wei Chao Zeng Guanghua Lei department of orthopaedics Xiangya HospitalCentral South UniversityChangsha 410008 HunanChina Hunan Key Laboratory of Joint Degeneration and Injury Changsha 410008 HunanChina Key Laboratory of Aging-related Bone and Joint Diseases Prevention and Treatment Ministry of EducationXiangya HospitalCentral South UniversityChangshaChina Division of Rheumatology Allergyand ImmunologyDepartment of MedicineMassachusetts General HospitalHarvard Medical SchoolBostonMA 02115USA The Mongan institute Massachusetts General HospitalHarvard Medical SchoolBostonMA 02115USA Academic Rheumatology School of MedicineUniversity of NottinghamNottingham NG51PBUK Pain Centre Versus Arthritis UK Nottingham NG51PBUK department of Endocrinology Endocrinology Research CenterXiangya HospitalCentral South UniversityChangsha 410008 HunanChina National Clinical research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangsha 410008 HunanChina Health Management Center Xiangya HospitalCentral South UniversityChangsha 410008 HunanChina department of Epidemiology and Health Statistics Xiangya School of Public HealthCentral South UniversityChangsha 410008 HunanChina

Hand osteoarthritis is a common heterogeneous joint disorder with unclear molecular mechanisms and no disease-modifying *** this study,we performed single-cell RNA sequencing analysis to compare the cellular composition and subpopulationspecific gene expression between cartilage with macroscopically confirmed osteoarthritis(n=5)and cartilage without osteoarthritis(n=5)from the interphalangeal joints of five *** 105142 cells,we identified 13 subpopulations,including a novel subpopulation with inflammation-modulating potential annotated as inflammatory *** chondrocytes exhibited extensive alteration of gene expression patterns in osteoarthritic cartilage compared with nonosteoarthritic *** inflammatory chondrocytes and fibrocartilage chondrocytes showed a trend toward increased numbers in osteoarthritic *** these two subpopulations from osteoarthritic cartilage,the ferroptosis pathway was enriched,and expression of iron overload-related genes,e.g.,FTH1,was *** verify these findings,we conducted a Mendelian randomization study using UK Biobank and a population-based cross-sectional study using data collected from Xiangya Osteoarthritis *** predisposition toward higher expression of FTH1 mRNA significantly increased the risk of hand osteoarthritis(odds ratio=1.07,95%confidence interval:1.02–1.11)among participants(n=332668)in UK *** levels of serum ferritin(encoded by FTH1),a biomarker of body iron overload,were significantly associated with a high prevalence of hand osteoarthritis among participants(n=1241)of Xiangya Osteoarthritis Study(P-for-trend=0.037).In conclusion,our findings indicate that inflammatory and fibrocartilage chondrocytes are key subpopulations and that ferroptosis may be a key pathway in hand osteoarthritis,providing new insights into the pathophysiology and potential therapeutic targets of hand osteoarthritis.

关键词： osteoarthritis subpopulations elevated

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Review: Bioengineering approach for the repair and regeneration of peripheral nerve

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Bioactive Materials 2019年第1期4卷 107-113页

作者： Joshua Moskow Bryan Ferrigno Nikhil Mistry Devina Jaiswal Ketan Bulsara Swetha Rudraiah Sangamesh G.Kumbar epartment of orthopaedic Surgery University of Connecticut Health263Farmington Ave.FarmingtonCT 06030USA department of Biomedical Engineering University of Connecticut260Glenbrook RoadUnit 3247StorrsCT 06269USA department of Surgery University of Connecticut Health263 Farmington Ave.FarmingtonCT 06030USA department of Pharmaceutical Sciences School of PharmacyUniversity of Saint Joseph229 Trumbull St.Hartford CT 06103USA

Complex craniofacial surgeries of damaged tissues have several limitations,which present complications and challenges when trying to replicate facial function and *** treatment techniques have shown suitable nerve function regeneration with various *** technology continues to advance,new methods have been explored in order to regenerate damaged nerves in an effort to more efficiently and effectively regain original function and *** article will summarize recent bioengineering strategies involving biodegradable composite scaffolds,bioactive factors,and external stimuli alone or in combination to support peripheral nerve *** emphasis is made on the contributions of growth factors and electrical stimulation on the regenerative process.

关键词： Peripheral nerve regeneration Composite materials Growth factor Electrical stimulation

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A novel classification method for mild adolescent idiopathic scoliosis using 3D ultrasound imaging

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Medicine in Novel Technology and Devices 2021年第3期11卷 151-161页

作者： D.Yang T.T.Y.Lee K.K.L.Lai Y.S.Wong L.N.Wong J.L.Yang T.P.Lam R.M.Castelein J.C.Y.Cheng Y.P.Zheng department of Biomedical Engineering The Hong Kong Polytechnic UniversityHong KongChina SH Ho Scoliosis research Lab Joint Scoliosis Research Center of the Chinese University of Hong Kong and Nanjing UniversityDepartment of Orthopaedics&TraumatologyThe Chinese University of Hong KongHong KongChina department of orthopaedic Surgery University Medical Center Utrechtthe Netherlands Spine Center Xinhua Hospital Affiliated to Shanghai Jiaotong University School of MedicineShanghaiChina

Mild adolescent idiopathic scoliosis(AIS),with Cobb<20°,was hypothesized as the right stage to intervene to prevent *** curve can be categorized into either structural or non-structural depending on the spine morphol... 详细信息

Mild adolescent idiopathic scoliosis(AIS),with Cobbresearch aimed at developing a mild AIs clasificationscheme through examining spine flexibility using 3D ultrasound *** the preliminary study,90 mild AIS subjects(21 M and 69 F;Age:14.5±1.7 years old;Cobb:18.2±6.4°)underwent both 3D ultrasound and X-ray scanning on the same *** each case,a clinician measured Cobbs and denoted major curve as ground *** Asymmetry Index(BAl)was developed to indicate the presence of a possible structural *** curve classification was coded to a modfied Lenke classification for mild cases(m-Lenke).The results of 3D ultrasound classification were evaluated with the *** was shown that 70.1%of the subjects had identical curve clssification results and 72.0%had the correct major curve ***-dominated curves had distinctive performance(p=0.91,r=0.91)against *** study demonstrated the possibility of a 3D ultrasound-based method for mild AIS curve *** could be partially explained by the limitations of the ultrasound scanning in proximal thoracicreg *** studies will validate the proposed method with a larger cohort.

关键词： AIS classification 3D ultrasound Mild AIS Spine flexibility

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3D cell-printing of gradient multi-tissue interfaces for rotator cuff regeneration

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Bioactive Materials 2023年第1期19卷 611-625页

作者： Suhun Chae Uijung Yong Wonbin Park Yoo-mi Choi In-Ho Jeon Homan Kang Jinah Jang Hak Soo Choi Dong-Woo Cho department of Mechanical Engineering Pohang University of Science and Technology77 Cheongam-roNam-guGyeongsangbuk-doPohang37673South Korea EDmicBio Inc. 111 Hoegi-roDongdaemun-guSeoul 02445South Korea institute for Convergence research and Education in Advanced Technology Yonsei University50 Yonsei-roSeodaemun-guSeoul03722South Korea Gordon Center for Medical Imaging Department of RadiologyMassachusetts General Hospital&Harvard Medical School14913th StreetBostonMA02114USA department of Convergence IT Engineering Pohang University of Science and Technology77 Cheongam-roNam-guGyeongsangbuk-doPohang37673South Korea School of Interdisciplinary Bioscience and Bioengineering Pohang University of Science and Technology77 Cheongam-roNam-guGyeongsangbuk-doPohang37673South Korea department of orthopaedic Surgery Asan Medical CenterCollege of MedicineUniversity of Ulsan86 Asanbyeongwon-gilSongpa-guSeoul05505South Korea

Owing to the prevalence of rotator cuff(RC)injuries and suboptimal healing outcome,rapid and functional regeneration of the tendon-bone interface(TBI)after RC repair continues to be a major clinical *** the essential role of the RC in shoulder movement,the engineering of biomimetic multi-tissue constructs presents an opportunity for complex TBI reconstruction after RC ***,we propose a gradient cell-laden multi-tissue construct combined with compositional gradient TBI-specific bioinks via 3D cell-printing *** vitro studies demonstrated the capability of a gradient scaffold system in zone-specific inducibility and multi-tissue formation mimicking *** regenerative performance of the gradient scaffold on RC regeneration was determined using a rat RC repair *** particular,we adopted nondestructive,consecutive,and tissue-targeted near-infrared fluorescence imaging to visualize the direct anatomical change and the intricate RC regeneration progression in real time in ***,the 3D cell-printed implant promotes effective restoration of shoulder locomotion function and accelerates TBI healing in *** summary,this study identifies the therapeutic contribution of cell-printed constructs towards functional RC regeneration,demonstrating the translational potential of biomimetic gradient constructs for the clinical repair of multi-tissue interfaces.

关键词： 3D cell-printing Tissue-specific bioink Gradient tissue scaffolds Near-infrared fluorophores Rotator cuff repair

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Cervical Spine （C4-C6） Test in Pure Bending Mode

Cervical Spine （C4-C6） Test in Pure Bending Mode

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第2届世界华人生物医学工程研讨会

作者： JCH Goh SL Toh PVS Lee department of orthopaedic Surgery National University of SingaporeDivision of Bioengineering National University of Singapore Division of Bioengineering National University of SingaporeDepartment of Mechanical Engineering National University of Singapore Division of Bioengineering National University of Singapore Defence Medical and Environmental Research Institute Defence Science Organisation

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Germinal center kinases in immune regulation

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Cellular & Molecular Immunology 2012年第6期9卷 439-445页

作者： Hailei Yin Zhubing Shi ShiJiao Cuicui Chen Wenjia Wang Mark I Greene Zhaocai Zhou department of Trauma and orthopaedics General Hospital of Jinan Military RegionJinanChina No.401 Hospital of People Liberation Army QingdaoChina State Key Laboratory of Cell Biology Institute of Biochemistry and Cell BiologyShangha nst tutes for Biological SciencesChinese Academy of SciencesShanghaiChina department of Pathology and Laboratory Medicine University of PennsylvaniaPhiladelphiaPAUSA

Germinal center kinases （GCKs） participate in a variety of signaling pathways needed to regulate cellular functions including apoptosis, cell proliferation, polarity and migration. Recent studies have shown that GCKs are participants in both adaptive and innate immune regulation. However, the differential activation and regulatory mechanisms of GCKs, as well as upstream and downstream signaling molecules, remain to be fully defined. It remains unresolved whether and how GCKs may cross-talk with existing signaling pathways. This review stresses the progresses in research of GCKs relevant to the immune system.

关键词： germinal center kinases immune regulation signal transduction

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Selective serotonin re-uptake inhibitor sertraline inhibits bone healing in a calvarial defect model

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International Journal of Oral Science 2018年第4期10卷 212-222页

作者： R.Nicole Howie Samuel Herberg Emily Durham Zachary Grey Grace Bennfors Mohammed Elsalanty Amanda C.LaRue William D.Hill James J.Cray Oral Health Sciences Medical University of South CarolinaCharlestonSCUSA Biomedical Engineering Case Western Reserve UniversityClevelandOHUSA Cellular Biology and Anatomy Augusta UniversityAugustaGAUSA Oral Biology Augusta UniversityAugustaGAUSA orthopaedic Surgery Augusta UniversityAugustaGAUSA Pathology and Laboratory Medicine Medical University of South CarolinaCharlestonSCUSA institute for Regenerative and Reparative Medicine Augusta UniversityAugustaGAUSA research Service of the Ralph H Johnson VA Medical Center CharlestonSCUSA Charlie Norwood VA Medical Center AugustaGAUSA department of Regenerative Medicine and Cellular Biology CharlestonSCUSA Wake Forest institute for Regenerative Medicine Wake Forest School of MedicineWinston-SalemNCUSA Division of Anatomy College of MedicineOhio State UniversityColumbusOHUSA

Bone wound healing is a highly dynamic and precisely controlled process through which damaged bone undergoes repair and complete regeneration. External factors can alter this process, leading to delayed or failed bone wound healing. The findings of recent studies suggest that the use of selective serotonin reuptake inhibitors(SSRIs) can reduce bone mass, precipitate osteoporotic fractures and increase the rate of dental implant failure. With 10% of Americans prescribed antidepressants, the potential of SSRIs to impair bone healing may adversely affect millions of patients’ ability to heal after sustaining trauma. Here, we investigate the effect of the SSRI sertraline on bone healing through pre-treatment with(10 mg·kg-1sertraline in drinking water, n = 26) or without(control, n = 30) SSRI followed by the creation of a 5-mm calvarial defect. Animals were randomized into three surgical groups:(a) empty/sham,(b) implanted with a DermaMatrix scaffold soak-loaded with sterile PBS or(c) DermaMatrix soak-loaded with542.5 ng BMP2. SSRI exposure continued until sacrifice in the exposed groups at 4 weeks after surgery. Sertraline exposure resulted in decreased bone healing with significant decreases in trabecular thickness, trabecular number and osteoclast dysfunction while significantly increasing mature collagen fiber formation. These findings indicate that sertraline exposure can impair bone wound healing through disruption of bone repair and regeneration while promoting or defaulting to scar formation within the defect site.

关键词： Selective serotonin re-uptake inhibitor sertraline inhibits bone healing in a calvarial defect model

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