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Cost of external fixation vs external fixation then nailing in bone infection

World Journal of Orthopedics 2015年第1期6卷 145-149页

作者： Khaled Mohamed Emara Ramy Ahmed Diab Khaled Abd EL Ghafar orthopaedic Surgery Ain Shams University

AIM: To study the cost benefit of external fixation vs external fixation then nailing in treatment of bone infection by segment ***: Out of 71 patients with infected nonunion tibia treated between 2003 and 2006, 50 patients fitted the inclusion criteria(26 patients were treated by external fixation only, and 24 patients were treated by external fixation early removal after segment transfer and replacement by internal fixation). Cost of inpatient treatment, total cost of inpatient and outpatient treatment till full healing, and the weeks of absence fromschool or work were calculated and compared between both ***: The cost of hospital stay and surgery in the group of external fixation only was 22.6 ± 3.3 while the cost of hospital stay and surgery in the group of early external fixation removal and replacement by intramedullary nail was 26.0 ± 3.2. The difference was statistically significant regarding the cost of hospital stay and surgery in favor of the group of external fixation only. The total cost of medical care(surgery, hospital stay, treatment outside the hospital including medications, dressing, physical therapy, outpatient laboratory work, etc.) in group of external fixation only was 63.3 ± 15.1, and total absence from work was 38.6 ± 6.6 wk. While the group of early removal of external fixation and replacement by IM nail, total cost of medical care was 38.3 ± 6.4 and total absence from work or school was 22.7 ± 4.1. The difference was statistically significant regarding the total cost and absence from work in favor of the group of early removal and replacement by IM ***: Early removal of external fixation and replacement by intramedullary nail in treatment of infected nonunion showed more cost effectiveness. orthopaedic society needs to show the cost effectiveness of different procedures to the community, insurance, and health authorities.

关键词： Cost Fixator Nailing Infection

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Niclosamide(NA)overcomes cisplatin resistance in human ovarian cancer

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Genes & Diseases 2023年第4期10卷 1687-1701页

作者： Linjuan Huang Jing Zhang Youling Deng Hao Wang Piao Zhao Guozhi Zhao Wei Zeng Yonghui Wang Connie Chen William Wagstaff Rex C.Haydon Russell R.Reid Tong-Chuan He Le Shen Hue H.Luu Ling Zhao Departments of Obstetrics and Gynecology Orthopaedic Surgery and UrologyThe First Affiliated Hospital of Chongqing Medical UniversityChongqing 400046China Molecular Oncology laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA Ministry of Education Key laboratory of Diagnostic Medicine and Department of Clinical BiochemistryThe School of Laboratory MedicineChongqing Medical UniversityChongqing 400016China Department of Neurology The Second Affiliated Hospital of Jianghan UniversityWuhanHubei 430050China Department of Clinical laboratory Medicine Shanghai Jiaotong University School of MedicineShanghai 200000China laboratory of Craniofacial Suture Biology and Development Department of Surgery Section of Plastic SurgeryThe University of Chicago Medical CenterChicagoIL 60637USA Department of Surgery The University of Chicago Medical CenterChicagoIL 60637USA

Ovarian cancer(OC)is one of the most lethal malignancies of the female reproduc-tive *** patients are usually diagnosed at advanced stages due to the lack of early *** standard treatment for OC includes a combination of debulking surgery and platinum-taxane chemotherapy,while several targeted therapies have recently been approved for maintenance *** vast majority of OC patients relapse with chemoresistant tu-mors after an initial ***,there is an unmet clinical need to develop new therapeu-tic agents to overcome the chemoresistance of *** anti-parasite agent niclosamide(NA)has been repurposed as an anti-cancer agent and exerts potent anti-cancer activities in human cancers including ***,we investigated whether NA could be repurposed as a therapeutic agent to overcome cisplatin-resistant(CR)in human OC *** this end,we first established two CR lines SKOV3CR and OVCAR8CR that exhibit the essential biological characteristics of cisplatin resistance in human *** showed that NA inhibited cell proliferation,sup-pressed cell migration,and induced cell apoptosis in both CR lines at a low micromole ***,NA inhibited multiple cancer-related pathways including AP1,ELK/SRF,HIF1,and TCF/LEF,in SKOV3CR and OVCAR8CR *** was further shown to effectively inhibit xenograft tumor growth of SKOV3CR ***,our findings strongly suggest that NA may be repurposed as an efficacious agent to combat cisplatin resistance in chemoresistant hu-man OC,and further clinical trials are highly warranted.

关键词： Chemotherapy resistance Cisplatin Drug repurposing Niclosamide Ovarian cancer

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Transcriptomic landscape regulated by the 14 types of bone morphogenetic proteins(BMPs)in lineage commitment and differentiation of mesenchymal stem cells(MSCs)

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Genes & Diseases 2019年第3期6卷 258-275页

作者： Linghuan Zhang Qing Luo Yi Shu Zongyue Zeng Bo Huang Yixiao Feng Bo Zhang Xi Wang Yan Lei Zhenyu Ye Ling Zhao Daigui Cao Lijuan Yang Xian Chen Bin Liu William Wagstaff Russell R*Reid Hue H*Luu Rex C*Haydon Michael J*Lee Jennifer Moriatis Wolf Zhou Fu Tong-Chuan He Quan Kang Stem Cell Biology and Therapy laboratory Ministry of Education Key Laboratory of Child Development and DisordersThe Children’s Hospital of Chongqing Medical UniversityChongqing 400014China Molecular Oncology laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA c Ministry of Education Key laboratory of Diagnostic Medicine School of Laboratory MedicineChicagoIL 60637USA d The Affiliated Hospitals of Chongqing Medical University Chongqing 400016China e Department of Clinical laboratory Medicine The Second Affiliated Hospital of Nanchang UniversityNanchang 330006China f Key laboratory of orthopaedic Surgery of Gansu Province Departments of Orthopaedic Surgery and Obstetrics and GynecologyThe First and Second Hospitals of Lanzhou UniversityLanzhou 730030China g Department of General Surgery The Second Affiliated Hospital of Soochow UniversitySuzhou 215004China Department of Clinical laboratory Medicine The Affiliated Hospital of Qingdao UniversityQingdao 266061China Department of Biology School of Life SciencesSouthwest UniversityChongqing 400715China Department of Surgery Section of Plastic SurgeryThe University of Chicago Medical CenterChicagoIL 60637USA

Mesenchymal stem cells(MSCs)are ubiquitously-existing multipotent progenitors that can self-renew and differentiate into multiple lineages including osteocytes,chondrocytes,adipocytes,tenocytes and *** represent one of the most commonly-used adult progenitors and serve as excellent progenitor cell models for investigating lineagespecific differentiation regulated by various cellular signaling pathways,such as bone morphogenetic proteins(BMPs).As members of TGFb superfamily,BMPs play diverse and important roles in development and adult *** least 14 BMPs have been identified in *** BMPs exert distinct but overlapping biological *** a comprehensive analysis of 14 BMPs in MSCs,we demonstrated that BMP9 is one of the most potent BMPs in inducing osteogenic differentiation of ***,a global mechanistic view of BMP signaling in regulating the proliferation and differentiation of MSCs remains to be fully ***,we conducted a comprehensive transcriptomic profiling in the MSCs stimulated by 14 types of *** clustering analysis classifies 14 BMPs into three subclusters:an osteo/chondrogenic/adipogenic cluster,a tenogenic cluster,and BMP3 *** also demonstrate that six BMPs(e.g.,BMP2,BMP3,BMP4,BMP7,BMP8,and BMP9)can induce ISmads effectively,while BMP2,BMP3,BMP4,BMP7,and BMP11 up-regulate Smad-independent MAP kinase ***,we show that many BMPs can upregulate the expression of the signal mediators of Wnt,Notch and PI3K/AKT/mTOR *** the reported transcriptomic changes need to be further validated,our expression profiling represents the first-of-its-kind to interrogate a comprehensive transcriptomic landscape regulated by the 14 types of BMPs in MSCs.

关键词： Bone morphogenetic proteins(BMPs) MAP kinase signaling Mesenchymal stem cells Notch signaling PI3K/AKT/mTOR pathway Smad signaling TGFb superfamily Wnt signaling

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Global trends and hot topics in clinical applications of perovskite materials:a bibliometric analysis

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Biomaterials Translational 2023年第3期4卷 131-141页

作者： Tai-Long Shi Yi-Fan Zhang Meng-Xuan Yao Chao Li Hai-Cheng Wang Chuan Ren Jun-Sheng Bai Xu Cui Wei Chen Department of orthopaedic Surgery Third Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina Key laboratory of Biomechanics of Hebei Province ShijiazhuangHebei ProvinceChina NHC Key laboratory of Intelligent orthopaedic Equipment ShijiazhuangHebei ProvinceChina Center for Human Tissues and Organs Degeneration Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina

In recent years,perovskite has received increasing attention in the medical ***,there has been a lack of related bibliometric analysis in this research *** study aims to analyse the research status and hot topics of perovskite in the medical field from a bibliometric perspective and explore the research direction of *** study collected 1852 records of perovskite research in the medical field from 1983 to 2022 in the Web of Science(WOS)*** country,institution,journal,cited references,and keywords were analysed using CiteSpace,VOS viewer,and Bibliometrix *** number of articles related to perovskite research in the medical field has been increasing every *** and USA have published the most papers and are the main forces in this research *** University of London Imperial College of Science,Technology,and Medicine is the most active institution and has contributed the most *** Applied Materials&Interfaces is the most prolific journal in this field.“Medical electronic devices”,“X-rays”,and“piezoelectric materials”are the most researched directions of perovskite in the medical field.“Performance”,“perovskite”,and“solar cells”are the most frequently used keywords in this *** Materials is the most relevant and academically influential journal for perovskite *** perovskites have been a hot topic in this field in recent years and will be a future research trend.X-ray,electronic medical equipment,and medical stents are the main research directions.

关键词： bibliometrics bibliometrix CiteSpace perovskite R package

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Corrigendum to “The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs” [Genes & Diseases 5 (2018) 62–74]

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Genes & Diseases 2023年第2期10卷 627-629页

作者： Zongyue Zeng Bo Huang Shifeng Huang Ruyi Zhang Shujuan Yan Xinyi Yu Yi Shu Chen Zhao Jiayan Lei Wenwen Zhang Chao Yang Ke Wu Ying Wu Liping An Xiaojuan Ji Cheng Gong Chengfu Yuan Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Rex C. Haydon Hue H. Luu Lan Zhou Russell R. Reid Tong-Chuan He Xingye Wu Ministry of Education Key laboratory of Diagnostic Medicine and School of laboratory Medicine Chongqing Medical UniversityChongqing 400016China Molecular Oncology laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA Department of Clinical laboratory Medicine The Second Affiliated Hospital of Nanchang UniversityNanchang 330006China Departments of Clinical laboratory Medicine General SurgeryOrthopedic SurgeryNephrologyand CardiologyThe First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China The Children's Hospital Chongqing Medical UniversityChongqing 400014China Department of laboratory Medicine and Clinical Diagnostics The Affiliated Yantai HospitalBinzhou Medical UniversityYantai 264100China Department of Immunology and Microbiology Beijing University of Chinese MedicineBeijing 100029China Key laboratory of orthopaedic Surgery of Gansu Province and the Department of orthopaedic Surgery The Second Hospital of Lanzhou UniversityLanzhou 730030China Department of Surgery The Affiliated Zhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Biochemistry and Molecular Biology China Three Gorges University School of MedicineYichang 443002China Department of orthopaedic Surgery Chongqing Hospital of Traditional Chinese MedicineChongqing 400021China Department of orthopaedic Surgery Xiangya Second Hospital of Central South UniversityChangsha 410011China Chongqing Key laboratory for Oral Diseases and Biomedical Sciences Chongqing 401147China The Affiliated Hospital of Stomatology of Chongqing Medical University Chongqing 401147China Department of Surgery Laboratory of Craniofacial Biology and DevelopmentSection of Plastic SurgeryThe University of Chicago Medical CenterChicagoIL 60637USA

The authors regret having an image assembly error in Figure 5Ca,in which the image for the "Oh dBiFP-AdRFp"group was erroneously duplicated with an overlapping image from the"36h BiFP dBIFP-AdR-simH19"*** confirm the error is restricted to the image assembly,and the underlying data and conclusions are correct and *** authors would like to apologize for any inconvenience caused.

关键词： image Figure unchanged

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Cu nanoparticle-decorated two-dimensional carbon nanosheets with superior photothermal conversion efficiency of 65 % for highly efficient disinfection under near-infrared light

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Journal of Materials Science & Technology 2021年第28期87卷 83-94页

作者： Jie Song Jun Li Xiangren Bai Liang Kang Liying Ma Naiqin Zhao Shuilin Wu Yuan Xue Jiajun Li Xiaojian Ji Junwei Sha School of Materials Science and Engineering and Tianjin Key laboratory of Composites and Functional Materials Tianjin UniversityTianjin300350China Department of orthopaedic Surgery Tianjin Medical University General HospitalTianjin300052China Department of Rheumatology Chinese PLA General HospitalBeijing100853China

Low photothermal conversion efficiency restricts the antibacterial application of photothermal *** this work,two-dimensional carbon nanosheets(2D C)were prepared and decorated with Cu nanoparticles(2D C/Cu)by using a simple soluble salt template method combined with ultrasonic *** photothermal conversion efficiency of 2 D C/Cu system can be optimized by changing the content of Cu nanoparticles,where the 2D C/Cu2 showed the best photothermal conversion efficiency(á)of 65.05%under 808 nm near-infrared light *** addition,the photothermal performance can affect the release behavior of Cu *** superior photothermal property combined with released Cu ions can endow this 2D hybrid material with highly efficient antibacterial efficacy of 99.97%±0.01%,99.96%±0.01%,99.97%±0.01%against Escherichia coli,Staphylococcus aureus,and methicillin-resistant Staphylococcus aureus,respectively,because of the synergetic effect of photothermy and ion *** addition,this 2D hybrid system exhibited good ***,this study provides a novel strategy to enhance the photothermal performance of 2D materials and thus will be beneficial for development of antibiotics-free antibacterial materials with safe and highly efficient bactericidal activity.

关键词： Photothermal conversion Antibacterial Two-dimensional carbon nanosheet Cu nanoparticle Near-infrared light

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Ultrabright NIR AIEgen nanoparticles-enhanced lateral flow immunoassay platform for accurate diagnostics of complex samples

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Aggregate 2024年第4期5卷 308-318页

作者： Jia Shu Yujian Li Huan Cai Qing Fu Chunyang Li Jianbo Yuan Yan Zhao Changjin Liu Haiping Wu Doudou Ling Zhangluxi Liu Guannan Su Qingfeng Cao Xiaolin Huang Rui Chen Peizeng Yang The First Affiliated Hospital of Chongqing Medical University Chongqing Branch(Municipality Division)of National Clinical Research Center for Ocular DiseasesChongqingChina Department of Orthopedics The First Affiliated Hospital of Chongqing Medical UniversityOrthopaedic Research LaboratoryChongqing Medical UniversityChongqingChina Key laboratory of Clinical laboratory Diagnostics(Ministry of Education) College of Laboratory MedicineChongqing Medical UniversityChongqingChina State Key laboratory of Food Science and Resources School of Food Science and TechnologyNanchang UniversityNanchangChina

Accurate and sensitive near-infrared(NIR)luminescent lateralﬂow immunoassay(LFIA)has attracted considerable attention in theﬁeld of point-of-care testing(POCT).However,the detection accuracy and sensitivity are often compromised by the lowﬂuorescence quantum efﬁciency of the NIRﬂuorescent probe.(laboratory-built NIR-LFIA portable quantitative instruments(detected light range 800 nm)completely eliminated background interference and allowed>highly accurate and sensitive detection without any pre-treatment *** limits of detection(LODs)for aﬂatoxin B1(AFB1)in soy sauce,alpha hemolysin(Hla)of Staphylococcus aureus biomarker in jointﬂuid,and C-reactive protein(CRP)in human haemolysed samples were 0.01 ng mL-1,0.02µg mL-1,and 0.156 mg L-1,respectively,commensurating with those of the corresponding gold standard assays and covering the detection range of *** is anticipated that the ultrabright NIR AIEgen nanoparticles will serve as a universally applicable signal probe for NIR-LFIA diagnostics,promising to expand the range of applications for quantitative detection of complex samples.

关键词： aggregation-induced emission complex sample lateralﬂow immunoassay near-infrared rapid diagnostic testing

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SV40 large T antigen-induced immortalization reprograms mouse cardiomyocyte progenitors with mesenchymal stem cell characteristics and osteogenic potential

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Genes & Diseases 2023年第4期10卷 1161-1164页

作者： Yichun Yu Jiamin Zhong Connie Chen Yannian Gou Guozhi Zhao Piao Zhao Yonghui Wang Wei Zeng Annie Wang William D.Wagstaff Jr Rex C.Haydon Tong-Chuan He Russell R.Reid Michael J.Lee Hue H.Luu Jiaming Fan Emergency Department Beijing Hospitaland The National Center of GerontologyInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijing 100051China Molecular Oncology laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA Ministry of Education Key laboratory of Diagnostic Medicine and the School of Laboratory Diagnostic MedicineChongqing Medical UniversityChongqing 400016China Departments of orthopaedic Surgery and Urology The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Clinical laboratory Medicine Shanghai Jiaotong University School of MedicineShanghai 200025China Department of Neurology The Second Affiliated Hospital of Jianghan UniversityWuhanHubei 430050China laboratory of Craniofacial Suture Biology and Development Department of Surgery Section of Plastic SurgeryThe University of Chicago Medical CenterChicagoIL 60637USA

While progenitor cell-based cardiomyocyte regeneration holds great promise of repairing an injured heart,primary cardiomyogenic progenitors(CPs)have a limited life span in culture,hampering the use of CPs for in vitro and in vivo *** previously isolated primary CPs from mouse E15.5 fetal heart,and reversibly immortalized them with SV40 large T antigen(SV40 LTA),resulting in immortalized CPs(iCPs),which maintain long-term proliferation and ex-press cardiomyogenic markers and retain differentiation potential under appropriate differentiation conditions.

关键词： progenitor cardio potential

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A botanical medicine dragon’s blood exhibited clinical antithrombosis efficacy similar to low molecular weight heparin

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Science China(Life Sciences) 2021年第10期64卷 1691-1701页

作者： Jiangbin Liang Song Mei Xiangyu Qiao Wei Pan Yan Zhao Shaohui Shi Yaling Zhai Haizhao Wen Guoping Wu Chengyu Jiang Department of orthopaedic Trauma Aviation General HospitalBeijing 100012China State Key laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical SciencesDepartment of BiochemistryPeking Union Medical CollegeBeijing 100005China

Deep vein thrombosis(DVT)is a common complication following traumatic fracture with a 0.5%–1%annual *** molecular weight heparin(LMWH)is the most commonly used anticoagulation drug for DVT prevention,but treatment with LMWH is *** aim is to compare the antithrombotic effect of dragon’s blood,an oral botanical anticoagulant medicine approved by the Chinese FDA,with LMWH in patients undergoing hip fracture surgery and to explore the molecular mechanisms of anticoagulation *** study recruited patients and divided them into LMWH and dragon’s blood treatment *** index tests,Doppler ultrasound and mRNA sequencing were performed before and after anticoagulation *** was no significant difference in postoperative DVT incidence between the two groups(23.1%versus 15.4%,P=0.694).D-dimer(D-D)and fibrinogen degradation product(FDP)showed significant reductions in both groups after anticoagulation *** identified SLC4A1,PROS1,PRKAR2B and seven other genes as being differentially expressed during anticoagulation therapy in both *** correlated with coagulation indexes were also ***’s blood and LMWH showed similar effects on DVT and produced similar gene expression changes in patients undergoing hip fracture surgery,indicating that dragon’s blood is a more convenient antithrombosis medicine(oral)than LMWH(hypodermic injection).

关键词： dragon’s blood low molecular weight heparin deep vein thrombosis transcriptome

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High formability Mg-Zn-Gd wire facilitates ACL reconstruction via its swift degradation to accelerate intra-tunnel endochondral ossification

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Journal of Magnesium and Alloys 2024年第1期12卷 295-315页

作者： Xuan He Ye Li Hongwei Miao Jiankun Xu Michael Tim-yun Ong Chenmin Wang Lizhen Zheng Jiali Wang Le Huang Haiyue Zu Zhi Yao Jie Mi Bingyang Dai Xu Li Patrick Shu-hang Yung Guangyin Yuan Ling Qin Musculoskeletal Research laboratory of Department of orthopaedics&Traumatology and Innovative orthopaedic Biomaterial and Drug Translational Research laboratory of Li Ka Shing Institute of Health Sciences The Chinese University of Hong KongHong KongChina National Engineering Research Center of Light Alloy Net Forming School of Materials Science&EngineeringShanghai Jiao Tong UniversityShanghaiChina Department of orthopaedics and Traumatology Li Ka Shing Faculty of MedicineThe University of Hong KongHong KongChina orthopaedic Department Peking University Third HospitalBeijingChina Department of Rehabilitation Sciences The Hong Kong Polytechnic UniversityHong KongChina

After reconstructing the anterior cruciate ligament(ACL),unsatisfactory bone tendon interface healing may often induce tunnel enlargement at the early healing *** good biological features and high formability,Magnesium-Zinc-Gadolinium(ZG21)wires are developed to bunch the tendon graft for matching the bone tunnel during ***,tensile strength,degradation,and cytotoxicity of ZG21 wire are *** rabbit model is used for assessing the biological effects of ZG21 wire by Micro-CT,histology,and mechanical *** SEM/EDS,immunochemistry,and in vitro assessments are performed to investigate the underlying *** tests demonstrate the high formability of ZG21 wire as surgical ***-CT shows ZG21 wire degradation accelerates tunnel bone formation,and histologically with earlier and more fibrocartilage regeneration at the healing *** mechanical test shows higher ultimate load in the ZG21 *** SEM/EDS presents ZG21 wire degradation triggered calcium phosphate(Ca-P)*** results demonstrate upregulation of Wnt3a,BMP2,and VEGF at the early phase and TGFβ3 and Type II collagen at the late phase of *** vitro tests also confirmed the Ca-P in the metal extract could elevate the expression of Wnt3a,βcatenin,ocn and opn to stimulate *** vivo tests of clinical samples indicated suturing with ZG21 wire did not weaken the ultimate loading of human tendon *** conclusion,the ZG21 wire is feasible for tendon graft *** degradation products accelerated intra-tunnel endochondral ossification at the early healing stage and therefore enhanced bone-tendon interface healing in ACL reconstruction.

关键词： Magnesium wire ACL reconstruction Magnesium alloy Biomaterials Endochondral ossification

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