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Neuroscience Bulletin 2006年第2期22卷 97-102页

作者： Jian-Zhong BAO Can-Rong NI Wei-Qiang ZHENG Department of Pathology Changhai Hospital Shanghai 200433 China Division of Reproductive Sciences Oregon Regional Primate Research Center Oregon Health ＆ Sciences University Beaverton OR 97006 USA

In the present study, we reported distribution of ERα and ER β mRNAs in the hypothalamus of young and old ovariectomized （OVX） rhesus macaques. The ERα were detected in all six major vestiblular nuclei which included arcuate nucleus （ARC） , paraventricularis nucleus （PVN） , periventricular nucleus （PeriV） , supraoptic nucleus （SON）, medial prioptic nucleus （MPN） and lateral hypotbalamus area （LHA）. However, the ERβ mRNA can also detected in those nuclei excerpt SON, but the signals of ERβ mRNA were weaker than those of ERα mRNA. We observed that the degree of expression of ERs mRNA were different in most nucleus of old and young monkeys. The ERα mRNAs were highly expressed in ARC and SON in young monkeys compared with old monkeys. Moderate amount of ERα mRNAs hybridization signals and weak signals were observed in LHA, and MPN both in young and old monkeys. In contrast, only lower level of ERα hybridization signal were observed in PVN and PeriV in young monkeys, and the signals of ERα were very low in those nucleus of old monkeys. In general, the expression of ERβ mRNA were weaker than that of ERα mRNA in above nucleus excerpt LHA. The relatively higher density of ERβ hybridization signals have been observed in the LHA in young monkey compared with old monkeys. Low amount of. ERβ mRNA hybridization signals were observed in the ARC, PVN and MPN, and no age differences were seen in PVN and MPN of those monkeys. In PeriV, we observed some signals in young monkey and a few signals in old monkeys. It was different from the rodent in which we did not found ERβ hybridization signal in SON. This study showed that both of the two estrogen receptors not only had the same pattern of expression but also had many different patterns of expression. The different expression of ERα and ERβ mRNAs in the young and old monkey brain may imply diverse functions in different regions of the monkey brain.

关键词： estrogen receptor mRNA hypothalamus Rhesus Macaque

Delivery strategies for novel vaccine formulations

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World Journal of Virology 2012年第1期1卷 4-10页

作者： Maria Trovato Shelly J Krebs Nancy L Haigwood Piergiuseppe De Berardinis Institute of Protein Biochemistry CNRNaples 80131 Italy Division of Pathobiology and Immunology Oregon National Primate Research Center and Vaccine and Gene Therapy Institute Oregon Health and Sciences UniversityBeaverton OR 97006 United States

A major challenge in vaccine design is to identify antigen presentation and delivery systems capable of rapidly stimulating both the humoral and cellular components of the immune system to elicit a strong and sustained immunity against different viral isolates. Approaches to achieve this end involve live attenuated and inactivated virions, viral vectors, DNA, and protein subunits. This review reports the state of current antigen delivery, and focuses on two innovative systems recently established at our labs. These systems are the filamentous bacteriophage fd and an icosahedral scaffold formed by the acyltransferase component(E2 protein) of the pyruvate dehydrogenase complex of Bacillus stearothermophilus.

关键词： Vaccines Antigen presentation Antigen delivery systems Filamentous bacteriophage fd E2 scaffold

FTY720,a sphingosine analog,altered placentome histoarchitecture in ewes

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Journal of Animal Science and Biotechnology 2020年第2期11卷 380-392页

作者： Kathrin A.Dunlap Bryan G.White David W.Erikson M.Carey Satterfield Christiane Pfarrer Guoyao Wu Fuller W.Bazer Robert C.Burghardt Kayla J.Bayless Greg A.Johnson Department of Animal Science Texas A&M UniversityCollege StationTX 77843USA Okanagan College Salmon Arm Campus Salmon ArmBritish ColumbiaCanada Endocrine Technologies Core Oregon National Primate Research CenterOregon Health&Science UniversityBeavertonORUSA Department of Anatomy University of Veterinary Medicine HannoverBischofsholer Damm 1530173 HannoverGermany Department of Veterinary Integrative Biosciences College of Veterinary Medicine and Biomedical SciencesTexas A&M UniversityCollege StationTX 77843USA Department of Molecular and Cellular Medicine Texas A&M Health Science CenterCollege StationTX 77843USA

Background:The lysosphingolipid,sphingosine-1-phosphate,is a well-described and potent pro-angiogenic ***,as well as the sphingosine phosphorylating enzyme sphingosine kinase 1,are expressed in the placentomes of sheep and the decidua of rodents;however,a function for this signaling pathway during pregnancy has not been *** objective of this study was to investigate whether sphingosine-1-phosphate promoted angiogenesis within the placentomes of pregnant *** were given daily jugular injections of FTY720(2-amino-2[2-(−4-octylphenyl)ethyl]propate-1,3-diol hydrochloride),an S1P ***:FTY720 infusion from days 30 to 60 of pregnancy did not alter maternal organ weights nor total number or mass of placentomes,but did alter placentome *** of caruncular crypts and cotyledonary villi was decreased,as was the relative area of cotyledonary tissue within ***,the percentage of area occupied by cotyledonary villi per unit of placentome was increased,while the thickness of the caruncular capsule was decreased in ewes treated with ***,FTY720 infusion decreased the number and density of blood vessels within caruncular tissue near the placentome capsule where the crypts emerge from the ***,FTY720 infusion decreased asparagine and glutamine in amniotic fluid and methionine in allantoic fluid,and decreased the crown rump length of day 60 ***:While members of the sphingosine-1-phosphate signaling pathway have been characterized within the uteri and placentae of sheep and mice,the present study uses FTY720 to address the influence of S1P signaling on placental *** present evidence that modulation of the S1P signaling pathway results in the alteration of caruncular vasculature,placentome architecture,abundance of amino acids in allantoic and amniotic fluids,and fetal growth during pregnancy in *** marked morphological changes in placentome histoarchitec

关键词： Placentome Pregnancy Sheep Sphingosine1 phosphate(S1P)

The Identity of Human Tissue-Emigrant CD8+T Cells

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四川生理科学杂志 2020年第4期42卷 426-426页

作者： Marcus Buggert center for Infectious Medicine Department of Medicine Huddinge Karolinska Institutet Karolinska University Hospital Huddinge Stockholm Sweden Department of Microbiology Perelman School of Medicine University of Pennsylvania Philadelphia PA USA Institute for Immunology Perelman School of Medicine University of Pennsylvania Philadelphia PA USA Division of Infectious Diseases Children’s Hospital of Philadelphia Philadelphia PA USA Department of Systems Pharmacology and Translational Therapeutics University of Pennsylvania Philadelphia PA USA Human Immunology Section Vaccine Research Center National Institute of Allergy and Infectious Diseases National Institutes of Health Bethesda MD USA Genome Analysis Core Vaccine Research Center National Institute of Allergy and Infectious Diseases National Institutes of Health Bethesda MD USA Neuroimmunology Unit Montreal Neurological Institute McGill University Montreal QC Canada Division of Human Genetics Children’s Hospital of Philadelphia Philadelphia PA USA center for Spatial and Functional Genomics Children’s Hospital of Philadelphia Philadelphia PA USA Vaccine and Gene Therapy Institute and oregon National primate research center Oregon Health & Science University Beaverton OR USA Department of Genetics Perelman School of Medicine University of Pennsylvania Philadelphia PA USA Division of Surgery Department of Clinical Science Intervention and Technology Karolinska Institutet Stockholm Sweden Division of Infection and Immunity Cardiff University School of Medicine Cardiff UK center for Neuroinflammation and Experimental Therapeutics Perelman School of Medicine University of Pennsylvania Philadelphia PA USA Department of Neurology Perelman School of Medicine University of Pennsylvania Philadelphia PA USA Division of Cardiology Department of Pediatrics Children’s Hospital of Philadelphia Philadelphia PA USA Department of Surgery Perelman School of Medicine University of Pennsylvania Philadelp

Lymphocyte migration is essential for adaptive immune ***,our current understanding of this process is rudimentary,because most human studies have been restricted to immunological analyses of blood and various *** address this knowledge gap,we used an integrated approach to characterize tissue-emigrant lineages in thoracic duct lymph(TDL).The most prevalent immune cells in human and non-human primate efferent lymph were T *** CD8+T cell subsets with effector-like epigenetic and transcriptional signatures were clonotypically skewed and selectively confined to the intravascular circulation,whereas non-cytolytic CD8+T cell subsets with stem-like epigenetic and transcriptional signatures predominated in tissues and ***,these anatomically distinct gene expression profiles were recapitulated within individual clonotypes,suggesting parallel differentiation programs independent of the expressed antigen *** collective dataset provides an atlas of the migratory immune system and defines the nature of tissue-emigrant CD8+T cells that recirculate via TDL.

关键词： signature collective Human