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Effect of EGb761 on light-damaged retinal pigment epithelial cells

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International Journal of ophthalmology(English edition) 2014年第1期7卷 8-13页

作者： Yun-Yun Zhou Chang-Zheng Chen Yu Su Lu Li Zuo-Hui-Zi Yi Hang Qi Ming Weng Yi-Qiao Xing department of ophthalmology Renmin Hospital of Wuhan University Zhongshan ophthalmic Center Sun Yat-sen University

AIM: To investigate the protective mechanism of Gingko Biloba extract(EGb761) on the ability of retinal pigment epithelial(RPE) cells to resist light-induced damage in a comparative proteomics study. · METHODS: Human RPE cells(ARPE-19) were randomly distributed to one of three groups: normal control(NC group) and light-damaged model without or with EGb761 group(M and ME groups,respectively). The light-damaged model was formed by exposing to white light(2 200 ±300)lx for 6h. The RPE cells in ME group were conducted with EGb761(100μg/mL) before light exposure. The soluble cellular proteins extracting from each groups were separated by two-dimensional electrophoresis and stained by silver staining. Different proteins in the profiles of the gels were analyzed by Image Master Software. Two-fold expressing protein spots were identified by Matrix-assisted laser desorption/ ionization tandem time-of-flight(MALDI-TOF/TOF) mass spectrometry. ·RESULTS: NC,M and ME groups displayed 1 892±71,2 145 ±23 and 2 216 ±85 protein spots,respectively. We identified 33 proteins with different expression levels between the NC and M groups,25 proteins between the M and ME groups,and 11 proteins between the NC and ME groups. MALDI-TOF/TOF mass spectrometry successfully identified 16 proteins,including metabolic enzymes,cytoskeletal proteins,anti-oxidation proteins,and others. ·CONCLUSION: Differences in some important proteins,such as cathepsin B,heat shock protein,and cytochrome C reductase,indicated that multiple pathways may be induced in light-damaged RPE cells and the protective effect of EGb761.

关键词： EGb761 retinal pigment epithelial cells light damage protein mass spectrographic analysis

Studies of a pedigree with limbal dermoid cyst

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International Journal of ophthalmology(English edition) 2012年第5期5卷 641-643页

作者： Jing Zhu, Xu-Yang Liu ophthalmic laboratories and department of ophthalmology Translational neuroscience Center West China Hospital Sichuan University Chengdu 610041 Sichuan Province China Shenzhen Eye Hospital Shenzhen 518000 Guangdong Province China

AIM: To study clinical features and gene mutations within the paired-like homeodomain transcription factor 2 (PITX2) gene in a pedigree of bilateral limbal dermoids. METHODS: Complete eye examinations have been performed on each individual of the family. Exons of paired-like homeodomain transcription factor 2 ( PITX2)were amplified by polymerase chain reaction, sequenced, and compared with a reference database. RESULTS: We described the phenotype, clinic findings in a family with two affected members. The masses of the proband's eyes were excised surgically demonstrating a dermoid cyst by histopathological examination. No mutation was detected in the gene PITX2 in this pedigree. CONCLUSION: A family of limbal dermoid cyst was reported. In addition, no pathogenic sequence variations were found in PITX2, indicating that this phenotype in this family is a distinctive entity.

关键词： Pedigree dermoid cyst limbus PITX2 gene

Morphological and immunocytochemical analysis of human retinal glia subtypes in vitro

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International Journal of ophthalmology(English edition) 2013年第5期6卷 559-563页

作者： Shao-Fen Lin Yu-Xiang Mao Bin Li Wei Sun Shi-Bo Tang Key Laboratory of ophthalmology Zhongshan Ophthalmic CenterSun Yat-Sen University department of ophthalmology the Affiliated Wuhan Tongji Hospital of Huazhong University of Science and Technology

AIMTo examine the morphological characteristics and antigen expression patterns of cultured human retinal glia to define novel subtypes.

关键词： retina glia morphology

Inhibition of retinopathy of prematurity in rat by intravitreal injection of sorafenib

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International Journal of ophthalmology(English edition) 2014年第2期7卷 198-204页

作者： Li-Li Tian Bing Ren Xiao-Wei Gao Ying Luo Yan Cai Kun Zhou An-Jie Du Yong Zhao Medical College of Shihezi University ophthalmic Center No.474 Hospital of Chinese PLA department of ophthalmology Yuncheng Central Hospital

AIM:To investigate the effect of intravitreal injection administered sorafenib,a multikinase inhibitor,in a rat model of oxygen-induced retinopathy(OIR).METHODS:Seven-day-old Sprague-Dawley rats(n=144)were randomly assigned to six *** A received normal partial oxygen pressure and groups B,C,D,E and F were exposed to hyperoxia(75±2)%from postnatal 7d(P7)to P12 to induce retinopathy of *** rats in groups C,D,E and F were received intravitreal injections of either vehicle(DMSO)or sorafenib at P12(5,20 and 80μg,respectively).Then they returned to normoxia after *** retinas were whole-mounted and imaged with a confocal *** vascular branching points were counted to quantify neovascularization at ***-sections of the retina were stained with hematoxylin and eosin(HE).The nuclei of new vessels breaking the internal limiting membrane were counted to quantify the proliferative neovascular ***:The retinal vessel in groups B and C turned into tortuosity and a great deal of neovascularization were ***-treated rats had significantly less neovascularization as compared with vehicle-treated and control rats in a dose dependent manner(P<0.05).The number of vascular branching points in A,B,C,D,E and F were 16.50±3.90,37.44±6.47,37.08±5.10,30.80±6.85,26.08±5.08 and 19.83±3.51,*** number of the nuclei of retinal new vessel in A,B,C,D,E and F were 0.22±0.42,35.66±4.70,35.30±4.54,27.30±4.28,21.41±3.53,and 7.41±2.87,*** were significant difference between each group(P<0.05)except groups B and ***:In the rat OIR model,sorafenib could inhibit retinal neovascularization in a dose dependent manner.

关键词： sorafenib retinopathy of prematurity neovascularization tyrosine kinase inhibitors

Changes in corneal biomechanics and intraocular pressure following Femto-LASIK using Goldman applanation tonometry and ocular response analyzer

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International Journal of ophthalmology(English edition) 2020年第5期13卷 782-787页

作者： Ahmed Yassin Hemida Omar M Said Asser A.E.Abdel-Meguid Mohammed Iqbal Amani E Badawi department of ophthalmology Fayoum Ophthalmic HospitalFayoum 63514Egypt department of ophthalmology Faculty of MedicineFayoum UniversityFayoum 63514Egypt department of ophthalmology Faculty of MedicineCairo UniversityCairo 11562Egypt department of ophthalmology Faculty of MedicineSohag UniversitySohag 8242Egypt department of ophthalmology Faculty of MedicineMansoura UniversityMansoura 35516Egypt

AIM:To compare intraocular pressure(IOP)measurements before and after laser in situ keratomileusis(LASIK)with a femtosecond laser for flap creation using ocular response analyzer(ORA)and Goldmann applanation tonometry,and to identify factors that may influence the preoperative and postoperative ***:A prospective study conducted on myopic patients who underwent LASIK using a femtosecond laser for flap *** patients were evaluated preoperatively,6 wk and 3 mo postoperatively for manifest refraction(MR),keratometric(K)readings and central corneal thickness(CCT)using a scheimpflug-based *** resistance factor(CRF),corneal hysteresis(CH),Goldmann correlated IOP(IOPg)and corneal compensated IOP(IOPcc)were measured using ORA besides IOP assessment by Goldman applanation tonometry(GAT).RESULTS:There was a statistically significant decrease in measures of IOPg by 3.35±0.83 mm Hg,followed by GAT which decreased by 2.2±0.44 mm Hg,and the least affected by operation was IOPcc which decreased only by 0.87±0.1 mm Hg after 6 *** 3 mo follow up there was a statistically significant decrease in IOPcc which decreased only by 0.76±0.4 mm Hg,followed by IOP GAT by 1.6±0.5 mm Hg,and the most affected by operation was IOPg which decreased by 2.3±0.3 mm ***,there was a statistically significant decrease in CH and CRF after 6 wk and 3 *** 3 mo,the preoperative MR and preoperative GAT were prominent significant predictors of the postoperative GAT *** prediction equation was ***:IOP measurements and corneal biomechanical factors reduce significantly after LASIK with a femtosecond laser for flap *** IOPcc values are less influenced by changes in corneal properties than IOPg and GAT,indicating that IOPcc may provide the most reliable measurement of IOP after this procedure.

关键词： intraocular pressure laser in situ keratomileusis femtosecond laser ocular response analyzer corneal biomechanics myopia

Cholinergic neuronal differentiation of bone marrow mesenchymal stem cells in rhesus monkeys

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Science China(Life Sciences) 2010年第5期53卷 573-580页

作者： QI Ying1,ZHANG FengYan2,SONG Ge1,SUN XueRong1,JIANG RuZhang1,CHEN MengFei1 & GE Jian1 1State Key Laboratory of ophthalmology,Zhongshan ophthalmic Center,Sun Yat-sen University,Guangzhou 510060,China 2department of ophthalmology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052,China State Key Laboratory of ophthalmology Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou China department of ophthalmology the First Affiliated Hospital Zhengzhou University Zhengzhou China

The purpose of the present study was to determine the best cholinergic neuronal differentiation method of rhesus monkey bone marrow mesenchymal stem cells(BMSCs).Four methods were used to induce differentiation,and the groups were assigned accordingly:basal inducing group(culture media,bFGF,and forskolin);SHH inducing group(SHH,inducing group);RA inducing group(RA,basal inducing group);and SHH+RA inducing group(SHH,RA,and basal inducing group).All groups displayed neuronal morphology and increased expression of nestin and neuron-specific *** basal inducing group did not express synapsin,and cells from the SHH inducing group did not exhibit neuronal resting membrane *** contrast,results demonstrated that BMSCs from the RA and SHH+RA inducing groups exhibited neuronal resting membrane potential,and cells from the SHH+RA inducing group expressed higher levels of synapsin and *** conclusion,the induction of cholinergic differentiation through SHH+RA was determined to be superior to the other methods.

关键词： bone marrow mesenchymal stem cells neuronal differentiation acetylcholine

Prevalence and characteristics of peripheral myopic retinopathy in Guangzhou office workers

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International Journal of ophthalmology(English edition) 2018年第8期11卷 1390-1395页

作者： Ting Zhang Yan-Tao Wei Wen-Bin Huang Rong-Jiao Liu Ya-Jing Zuo Li-Wen He Li-Ting Zhong Shao-Chong Zhang State Key Laboratory of ophthalmology Zhongshan Ophthalmic CenterSun Yat-Sen University department of ophthalmology the Second Affiliated Hospital of Guangzhou Medical University

AIM： To determine the prevalence and characteristics of peripheral myopic retinopathy among a sample of Guangzhou office workers. METHODS： A cross-sectional study of Guangzhou Chinese office works in different departments and units of the Guangzhou Power Supply Bureau, China, in 2016. Myopic retinopathy was recorded and analyzed with a scanning laser ophthalmoscope and by slit-lamp microscopy combined with a three-mirror contact lens. RESULTS： In total, 1910 eyes of 955 subjects（508 females and 447 males） aged 21-59 y were included; 69.6% of these eyes were myopic. The myopia group had a younger age and worse uncorrected visual acuity（UCVA） and bestcorrected visual acuity（BCVA） when compared with hyperopia and emmetropia groups（P〈0.001）. The axial length（AL） was significantly longer, the spherical equivalent（SE） was more serious, and the optic nerve crescent was significantly larger in subjects with myopia than with hyperopia and emmetropia. Subjects with myopia, and especially high myopia, had the highest frequency of myopic retinal 18 changes（49.4%, P〈0.001） [white-without-pressure（43.8%, P〈0.001）, lattice degeneration（4.5%, P=0.044）] among the three groups. Logistic regression confirmed that any myopia（OR： 3.41, P〈0.001） [mild myopia（OR： 1.93, P=0.001）, moderate myopia（OR：3.64, P〈0.001）, and high myopia（OR：10.58, P〈0.001）], a greater AL（OR： 1.55, P〈0.001） and a much higher SE（OR： 0.77, P〈0.001） increased the risk for peripheral retinal ***： Myopia-related retinal changes are positively associated with greater AL, higher SE, and myopia.

关键词： peripheral myopic retinopathy prevalence characteristics office workers

Evaluation of fundus autofluorescence patterns in age-related macular degeneration

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International Journal of ophthalmology(English edition) 2016年第12期9卷 1779-1784页

作者： Pradeep Venkatesh Pradeep Sagar Rohan Chawla Varun Gogia Rajpal Vohra Yog Raj Sharma department of ophthalmology Dr. Rajendra Prasad Centre for Ophthalmic SciencesAll India Institute of Medical Sciences

AIM： To study the various morphological patterns of fundus autofluorescence （FAF） images in patients with age-related macular degeneration （AMD） in Indian ***： Totally 179 eyes of 104 patients with clinical diagnosis of AMD were recruited into the study. Autofluorescence images were captured using confocal scanning laser ophthalmoscope and the patterns of FAF were ***： Of 179 eyes, 27 （15.08%） were early AMD, 58 （32.41%） were intermediate AMD, 94 eyes （52.51%） were late AMD. Of 94 eyes with late AMD, 79 （84.04%） were neovascular AMD and 15 （15.96%） were central geographic atrophy. In eyes with early and intermediate AMD, 9 patterns of FAF were noted. Six patterns （normal, minimal change, focal increased, patchy increased, linear, reticular） were similar to that in the published classification. Two patterns （lacelike and speckled） described in the published classification were not found. Three new patterns （focal hypo-fluorescence, patchy hypo-fluorescence, mixed focal hypo-fluorescence and hyper-fluorescence） were detected. In eyes with neovascular AMD, 6 morphological patterns of FAF were noted. Two patterns （mixed hypo-fluorescence and hyper-fluorescence, central hypo-fluorescence with hyper-fluorescent rim） were similar to that in published classification. Two patterns （normal, near normal or normal background fluorescence in the centre of hypo-fluorescent area） described in the published classification were not found. Four new patterns （minimal change, hypo-fluorescent patch, central hypo-fluorescence with surrounding reticular, bull’s eye） were recognized. In eye with central geographic atrophy 5 morphological patterns were noted and these were similar to that in published ***： Phenotypic differences in the pattern of FAF exist in the study population compared to existing classification systems.

关键词： age-related macular degeneration fundus autofluorescence lipofuscin choroidal neovascularization

Diagnostic ability of ganglion cell complex thickness to detect glaucoma in high myopia eyes by Fourier domain optical coherence tomography

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International Journal of ophthalmology(English edition) 2018年第5期11卷 791-796页

作者： Wei-Wei Wang Huai-Zhou Wang Jian-Rong Liu Xi-Fang Zhang Meng Li Yan-Jiao Huo Xin-Guang Yang department of ophthalmology Xi'an No.4 HospitalShaanxi Ophthalmic Medical CenterAffiliated Guangren Hospital School of MedicineXi'an Jiaotong University department of ophthalmology Beijing Tongren HospitalCapital Medical University

AIM：To evaluate the ability of macular ganglion cell complex（GCC） thickness using Fourier domain optical coherence tomography（FD-OCT） to detect glaucoma in highly myopic ***：Cross-sectional study.A total of 114 participants,consecutively were *** GCC thickness and peripapillary retinal nerve fiber layer（RNFL） thickness were obtained with RTVue *** operating characteristics curves were constructed for each measurement parameter,and areas under the curves（AUCs） were ***：Both the average GCC and average RNFL thickness showed negative correlations with axial length（rGCC=-0.404,P=0.001;rRNFL=-0.561,P〈0.001）.The largest AUCs from GCC,and RNFL parameters were 0.968 [global loss volume（GLV）],and 0.855（average RNFL）,*** was significantly better for detecting high myopic glaucoma than average RNFL（P〈0.001）.CONCLUSION：Macular GCC thickness has higher diagnostic power than peripapillary RNFL thickness to discriminate glaucoma patients from non-glaucoma subjects in high myopia.

关键词： optical coherence tomography glaucoma high myopia ganglion cell complex