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Superhydrophobic Surfaces Produced by Applying a Self- Assembled Monolayer to Silicon Micro/Nano-Textured Surfaces

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Nano Research 2009年第2期2卷 143-150页

作者： Yong Song Rahul Premachandran Nair Min Zou Yongqiang Wang Department of Mechanical Engineering University of ArkansasFayettevilleAR 72701USA ocean nanotech LLC.2143 Worth LaneSpringdaleAR 72764USA

A novel way of producing superhydrophobic surfaces by applying a self-assembled monolayer(SAM)to silicon micro/nano-textured surfaces is presented in this *** micro/nano-textured surfaces on silicon substrates were generated by the aluminum-induced crystallization(AIC)of amorphous silicon(a-Si)***(OTS)SAMs were then applied to the textured surfaces by dip *** topography and wetting properties of the resulting surfaces were characterized using scanning electron microscopy(SEM)and a video-based contact angle measurement *** results show that by introducing OTS SAMs on the silicon micro/nano-textured surfaces,superhydrophobic surfaces with water contact angles(WCAs)of 155°were obtained,as compared to the WCAs of OTS-modified smooth silicon surfaces of about 112°.Surface topography was found to directly influence the WCA as predicted by the Cassie-Baxter model.

关键词： Superhydrophobic wetting property micro/nano-textured surfaces octadecyltrichlorosilane self-assembled monolayer aluminum-induced crystallization of amorphous silicon

Expression and Characterization of ArgR, An Arginine Regulatory Protein in Corynebacterium crenatum

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Biomedical and Environmental Sciences 2014年第6期27卷 436-443页

作者： CHEN Xue Lan ZHANG Bin TANG Li JIAO Hai Tao XU Heng Yi XU Feng XU Hong WEI Hua XIONG Yong Hua Key Laboratory of Functional Small Organic Molecule Ministry of Education Jiangxi Normal University State Key Laboratory of Food Science and Technology Nanchang University ocean nanotech LLC

Objective Corynebacterium crenatum MT, a mutant from C. crenatum AS 1.542 with a lethal argR gene, exhibits high arginine production. To confirm the effect of ArgR on arginine biosynthesis in C. crenatum, an intact argR gene from wild-type AS 1.542 was introduced into C. crenatum MT, resulting in C. crenatum MT. sp, and the changes of transcriptional levels of the arginine biosynthetic genes and arginine production were compared between the mutant strain and the recombinant strain. Methods Quantitative real-time polymerase chain reaction was employed to analyze the changes of the related genes at the transcriptional level, electrophoretic mobility shift assays were used to determine ArgR binding with the argCJBDF, argGH, and carAB promoter regions, and arginine production was determined with an automated amino acid analyzer. Results Arginine production assays showed a 69.9% reduction in arginine from 9.01±0.22 mg/mL in C. crenatum MT to 2.71±0.13 mg/mL （P〈0.05） in C. crenatum MT. sp. The argC, argB, argD, argF, argJ, argG, and carA genes were down-regulated significantly in C. crenatum MT. sp compared with those in its parental C. crenatum MT strain. The electrophoretic mobility shift assays showed that the promoter regions were directly bound to the ArgR protein. Conclusion The arginine biosynthetic genes in C crenatum are clearly controlled by the regulator ArgR, and intact ArgR in C. crenatum MT results in a significant descrease in production. negative arginine production.

关键词： Corynebacterium crenatum ArgR protein Arginine biosynthetic genes Real-time PCR ElectrophoretJc mobility shift assay

In vitro cytotoxicity of CdSe/ZnS quantum dots with different surface coatings to human keratinocytes HaCaT cells

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Journal of Environmental Sciences 2013年第1期25卷 163-171页

作者： Kavitha Pathakoti Huey-Min Hwang Hong Xu Zoraida P.Aguilar Andrew Wang Department of Biology Jackson State University ocean nanotech 2143 Worth LaneSpringdale Arkansas 72764KUSA

Quantum dots （QD） nanoparticles have been widely used in biomedical and electronics fields, because of their novel optical properties. Consequently it confers enormous potential for human exposure and environmental release. To increase the biocompatibility of QDs, a variety of surface coatings or functional groups are added to increase their bioactivity and water solubility. Human adult low calcium high temperature （HaCaT） cells are the epithelial cells derived from adult human skin that exhibits normal differentiation capacity and a DNA fingerprint pattern that is unaffected by long-term cultivation, transformation, or the presence of multiple chromosomal alternations. Human keratinocytes, HaCaT cells were used to systematically evaluate the cytotoxicity of biocompatible QD made of CdSe metal core and ZnS shell with three different coatings and at three different wavelengths （530, 580 and 620 nm）. In terms of half- maximal inhibitory concentration, QSA-QDs with amine-polyethyleneglycol coating and QSH-QDs with amphiphilic polymer coating were not cytotoxic, while QEI-QDs with polyethylenimine coating were highly toxic to the HaCaT cells in comparison to a reference CulnS2/ZnS. QEI-QDs led to significant increase in reactive oxygen species, decrease in mitochondrial membrane potential and DNA damage in HaCaT cells. The mechanisms of toxicity of QEI-530 and QEI-580 can be attributed to the combination of intracellular reactive oxygen species production and loss of MMP. The QDs toxicity can be attributed to the polyethylemimine surface coating which was highly toxic to cells in comparison with amine-polyethyleneglycol, but not due to the release of cadmium ions.

关键词： nanoparticles quantum dots HaCaT cytotoxicity genotoxicity

Preparation and Control of the Formation of Single Core and Clustered Nanoparticles for Biomedical Applications Using a Versatile Amphiphilic Diblock Copolymer

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Nano Research 2010年第12期3卷 852-862页

作者： Hongwei Chen Julie Yeh Liya Wang Hafsa Khurshid Nan Peng Andrew YWang Hui Mao Department of Radiology Center for Systems ImagingEmory University School of MedicineAtlantaGeorgia 30322USA Department of Physics and Astronomy University of DelawareNewarkDelaware 19716USA Department of Rehabilitation Medicine Chinese PLA General Hospital28 Fuxing RoadBeijing 100853China ocean nanotech LLC2143 Worth LaneSpringdaleArkansas 72764USA

We report the application of a versatile diblock copolymer,poly(ethylene oxide)-b-poly(γ-methacryloxypropyl trimethoxysilane)(PEO-b-PγMPS),to prepare nanocrystals such as iron oxide nanoparticles or quantum dots,with either a single core or multi-core cluster,for biomedical *** amphiphilic copolymer comprises both a hydrophilic PEO segment and a hydrophobic segment with a“surface anchoring moiety”(the silane group)which can interact effectively with the hydrophobic nanocrystals through ligand *** of the unique features of this work is that we can control the formation of either single core nanoparticles or multi-core nanoclusters by simply varying the conditions of ligand exchange and aging of the mixture of block copolymer and nanoparticles without needing to change the *** morphologies of the resulting single core nanoparticles or multi-core nanoclusters were confirmed by dynamic light scattering and transmission electron *** clustered nanoparticles exhibit enhanced physicochemical properties that are beyond those expected from a simple accumulation of individual ***,the hybrid nanoparticles containing both magnetic iron oxide nanoparticles and optical quantum dots obtained using our strategy provide have combined magnetic and optical functionalities that allow for potential new and expanded biomedical applications,as demonstrated by their use for magnetic resonance imaging and biomarker-targeted cell imaging.

关键词： Magnetic nanoparticles quantum dots nanoparticle coating copolymer multifunctional nanoparticles imaging

IN SITU IMAGING OF BREAST CANCER CELLS USING GREEN SEMICONDUCTOR QUANTUM DOTS

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Transactions of Nanjing University of Aeronautics and Astronautics 2010年第1期27卷 13-20页

作者：许恒毅 Zoraida P. Aguilar 苏怀朋 Benjamin J. Jones John. D. Dixon 熊勇华魏华 Andrew Y. Wang 南昌大学食品科学与技术国家重点实验室 ocean nanotech llc 2143 Worth LaneSpringdaleusa72764

The breast cancer is the most common cause of cancer death in women. To establish an early stage in situ imaging of breast cancer cells, green quantum dots （QDs） are used as a fluorescent signal generator. The QDs based imaging of breast cancer cells involves anti-HER2/neu antibody for labeling the over expressed HER2 on the surface of breast cancer cells. The complete assay involves breast cancer cells, biotin labeled antibody and streptavidin conjugated QDs. The breast cancer cells are grown in culture plates and exposed to the biotin labeled antibodies, and then exposed to streptavidin labeled QDs to utilize the strong and stable biotin-streptavidin interaction. Fluorescent images of the complete assay for breast cancer cells are evaluated on a microscope with a UV light source. Results show that the breast cancer cells in the complete assay are used as fluorescent cells with brighter signals compared with those labeled by the organic dye using similar parameters and the same number of cells.

关键词： in situ processing quantum optics breast cancer cells non-specific binding immuno-histochemical imaging

Dynamic Analysis for the Global Performance of An SPM-Feeder-Cage System Under Waves and Currents

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China ocean Engineering 2015年第3期29卷 415-430页

作者： Cristian CIFUENTES M.H.KIM ocean Engineering Program Department of Civil Engineering Texas A&M University College Station

In the present study, the dynamic response of a coupled SPM-feeder-cage system under irregular waves and shear currents is analyzed. A numerical model is developed by using the commercial software Orca Flex. Hydrodynamics coefficients of the vessel are calculated by using a 3D diffraction/radiation panel program. First- and second-order wave forces are included in the calculations. Morison equation is used to compute the drag force on line elements representing the net. Drag coefficients are determined at every time step in the simulation considering the relative normal velocity between the structural elements and the fluid flow. The dynamic response of the coupled system is analyzed for various environments and net materials. The results of the study show the effects of solidity ratio of the net and vertical positions of the cage on the overall dynamic response of the system, confirming the viability of this type of configuration for future development of offshore aquaculture in deep waters.

关键词： offshore aquaculture SPM(single point mooring) feeder vessel connecting lines operational/survival environment drag force equivalent net cage grouping net modeling line tensions solidity ratio

量子点荧光微球免疫层析试纸条定量检测恶性疟原虫

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分析化学 2015年第3期43卷 338-343页

作者：段宏陈雪岚江湖沈骏董胜明熊勇华 Andrew Wang 南昌大学食品科学与技术国家重点实验室江西师范大学生命科学学院北京金源佳和生物科技有限公司 4. ocean nanotech LLC.San Diego CA92126 USA

以羧基化Cd Te/Zn Se量子点荧光微球为荧光标记物,采用1-乙基-(3-二甲基氨基丙基)碳酰二亚胺(EDC)法偶联抗恶性疟原虫富组氨酸蛋白(Pf)单克隆抗体制备荧光探针;以羊抗恶性疟原虫组氨酸多克隆抗体和驴抗鼠二抗分别喷涂硝酸纤维膜,形成试... 详细信息

以羧基化Cd Te/Zn Se量子点荧光微球为荧光标记物,采用1-乙基-(3-二甲基氨基丙基)碳酰二亚胺(EDC)法偶联抗恶性疟原虫富组氨酸蛋白(Pf)单克隆抗体制备荧光探针;以羊抗恶性疟原虫组氨酸多克隆抗体和驴抗鼠二抗分别喷涂硝酸纤维膜,形成试纸条检测线和质控线,建立了免疫层析试纸条定量检测血清中恶性疟原虫的方法。所使用的羧基化量子点荧光微球的荧光强度为单个量子点的2800倍。实验结果表明,该荧光试纸条定量检测血清中恶性疟原虫线性范围为5.8~8010 Parasite/μL,最低灵敏度达到5.8 Parasite/μL,单个样品检测时间只需15 min。加标回收实验显示,试纸条批内回收率为93.0%~111.8%,批间回收率为98.3%~115.1%,且批内、批间的相对标准偏差均小于5%。

关键词：量子点荧光微球免疫层析试纸条恶性疟原虫定量检测

Interface Engineered Microcellular Magnetic Conductive Polyurethane Nanocomposite Foams for Electromagnetic Interference Shielding

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Nano-Micro Letters 2021年第10期13卷 131-146页

作者： Guolong Sang Pei Xu Tong Yan Vignesh Murugadoss Nithesh Naik Yunsheng Ding Zhanhu Guo Department of Polymer Science and Engineering School of Chemistry and Chemical Engineeringand Anhui Key Laboratory of Advanced Functional Materials and DevicesHefei University of TechnologyHefei 230009People’s Republic of China Advanced Materials Division Engineered Multifunctional Composites(EMC)Nanotech.LLCKnoxvilleTN 37934USA Integrated Composites Laboratory(ICL) Department of Chemical and Biomolecular EngineeringUniversity of TennesseeKnoxvilleTN 37996USA Department of Mechanical and Manufacturing Engineering Manipal Institute of TechnologyManipal Academy of Higher EducationManipalKarnataka 576104India

Lightweight microcellular polyurethane(TPU)/carbon nanotubes(CNTs)/nickel-coated CNTs(Ni@CNTs)/polymerizable ionic liquid copolymer(PIL)composite foams are prepared by non-solvent induced phase separation(NIPS).CNTs and Ni@CNTs modified by PIL provide more heterogeneous nucleation sites and inhibit the aggregation and combination of microcellular *** with TPU/CNTs,the TPU/CNTs/PIL and TPU/CNTs/Ni@CNTs/PIL composite foams with smaller microcellular structures have a high electromagnetic interference shielding effectiveness(EMI SE).The evaporate time regulates the microcellular structure,improves the conductive network of composite foams and reduces the microcellular size,which strengthens the multiple reflections of electromagnetic *** TPU/10CNTs/10Ni@CNTs/PIL foam exhibits slightly higher SE values(69.9 dB)compared with TPU/20CNTs/PIL foam(53.3 dB).The highest specific EMI SE of TPU/20CNTs/PIL and TPU/10CNTs/10Ni@CNTs/PIL reaches up to 187.2 and 211.5 dB/(g cm^(−3)),*** polarization losses caused by interfacial polarization between TPU substrates and conductive fillers,conduction loss caused by conductive network of fillers and magnetic loss caused by Ni@CNT synergistically attenuate the microwave energy.

关键词： Polyurethane Nanocomposites Microcellular Electromagnetic interference shielding

Simulations of Eurasian Winter Temperature Trends in Coupled and Uncoupled CFSv2

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Advances in Atmospheric Sciences 2018年第1期35卷 14-26页

作者： Thomas W.COLLOW Wanqiu WANG Arun KUMAR INNOVIM LLC NOAA/NCEP Climate Prediction Center College Park

Conflicting results have been presented regarding the link between Arctic sea-ice loss and midlatitude cooling, particularly over Eurasia. This study analyzes uncoupled(atmosphere-only) and coupled(ocean–atmosphere) simulations by the Climate Forecast System, version 2(CFSv2), to examine this linkage during the Northern Hemisphere winter, focusing on the simulation of the observed surface cooling trend over Eurasia during the last three decades. The uncoupled simulations are Atmospheric Model Intercomparison Project(AMIP) runs forced with mean seasonal cycles of sea surface temperature(SST)and sea ice, using combinations of SST and sea ice from different time periods to assess the role that each plays individually,and to assess the role of atmospheric internal variability. Coupled runs are used to further investigate the role of internal variability via the analysis of initialized predictions and the evolution of the forecast with lead *** AMIP simulations show a mean warming response over Eurasia due to SST changes, but little response to changes in sea ice. Individual runs simulate cooler periods over Eurasia, and this is shown to be concurrent with a stronger Siberian high and warming over Greenland. No substantial differences in the variability of Eurasian surface temperatures are found between the different model configurations. In the coupled runs, the region of significant warming over Eurasia is small at short leads, but increases at longer leads. It is concluded that, although the models have some capability in highlighting the temperature variability over Eurasia, the observed cooling may still be a consequence of internal variability.

关键词： Eurasian winter climate sea-ice loss SST increase CFSv2