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Journal of Mountain Science 2016年第11期13卷 2046-2052页

作者： LI Qiang LI Shu-hong HUANG Wen-li LIU Cheng-yi XIONG Chuan LI Xiao-lin ZHENG Lin-yong Soil and Fertilizer Institute Sichuan Academy of Agricultural SciencesChengdu 610066China biotechnology&Germplasm Resources Institute Yunnan Academy of Agricultural SciencesKunming 650221China Institute of Biological&nuclear Technology Sichuan Academy of Agricultural SciencesChengdu 610066China Sichuan Panzhihua Academia of agriculture and Forestry Panzhihua 617061China

Fruiting bodies(500 g per site) of Tricholoma matsutake and the surface layer of soils collected from 20 spatially distant areas with pristine backgrounds across China were analyzed for potassium,magnesium,calcium,zinc,iron,copper,manganese and cadmium using inductively coupled plasma optical emission *** terms of the bioconcentration and bioexclusion concept,K,Mg,Zn,and Cu were highly bioconcentrated,and their bioconcentration factor values varied between 75-615,2-107,38-603 and 7-76,respectively,across the 20 ***,Mn and Cd were moderately bioconcentrated and their bioconcentration factors(BCFs) varied between 0.6-34.0,0.4-37.0 and 0.9-7.0 ***,Ca was excluded(BCF<1).*** is a species that is harvested in the wild as a valuable food and can contain a wide spectrum of both essential and hazardous mineral compounds that accumulate at elevated concentrations even if grown in pristine *** estimated intake rate of Cd in the fruiting bodies indicates cause for concern associated with this metal resulting from the daily consumption of between 200 and 400 g of fruiting bodies on a frequent basis during the mushrooming season.

关键词： Fungi Heavy metals Mineral composition Mushrooms Wild food

A pair of nuclear factor Y transcription factors act as positive regulators in jasmonate signaling and disease resistance in Arabidopsis

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Journal of Integrative Plant Biology 2024年第9期66卷 2042-2057页

作者： Chuyu Lin Chenghao Lan Xiaoxiao Li Wei Xie Fucheng Lin Yan Liang Zeng Tao Ministry of agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects Institute of BiotechnologyZhejiang UniversityHangzhou 310058China State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro‐products Institute of BiotechnologyZhejiang UniversityHangzhou 311400China

The plant hormone jasmonate(JA)regulates plant growth and immunity by orchestrating a genome-wide transcriptional *** the resting stage,JASMONATE-ZIM DOMAIN(JAZ)proteins act as main repressors to regulate the expression of JA-responsive genes in the JA signaling ***,the mechanisms underlying de-repression of JA-responsive genes in response to JA treatment remain ***,we report two nuclear factor Y transcription factors NF-YB2 and NF-YB3(thereafter YB2 and YB3)play key roles in such de-repression in ***2 and YB3 function redundantly and positively regulate plant resistance against the necrotrophic pathogen Botrytis cinerea,which are specially required for transcriptional activation of a set of JA-responsive genes following ***,YB2 and YB3 modulated their expression through direct occupancy and interaction with histone demethylase Ref6 to remove repressive histone ***,YB2 and YB3 physically interacted with JAZ repressors and negatively modulated their abundance,which in turn attenuated the inhibition of JAZ proteins on the transcription of JA-responsive genes,thereby activating JA response and promoting disease ***,our study reveals the positive regulators of YB2 and YB3 in JA signaling by positively regulating transcription of JA-responsive genes and negatively modulating the abundance of JAZ proteins.

关键词： histone demethylase jasmonate response JAZ repressor necrotrophic resistance nuclear factor

Water use efficiencies of maize cultivars grown under rain-fed conditions

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Agricultural Sciences 2011年第2期2卷 125-130页

作者： Daniel Kwasi Asare Justice Okona Frimpong Emmanuel Ofori Ayeh Harry Mensah Amoatey Department of Plant and Soil Sciences Biotechnology and Nuclear Agriculture Research Institute Ghana Atomic Energy Commission Legon-Accra Ghana

Enhancing water use efficiencies of rain-fed maize is a requirement for sustainable maize production, particularly in areas prone to low/drought and erratic rainfall patterns. This study was conducted to assess the relationship between total biomass/grain yield and water use efficiencies of three maize cultivars (Golden Crystal, Mamaba and Obatanpa) grown under rain-fed conditions in a coastal savannah agro-ecological environment of Ghana. Results of the study showed that a unified linear model, WUETDM = 0.03TDM with R2 = 0.765 and P ≤ 0.001, described adequately the relation between wa-ter use efficiency and total biomass (dry matter), which is applicable for the three maize cultivars for both the major and minor cropping seasons. A linear model could only, however, describe adequately well the relation between WUEGY and GY for the major (WUEGY = 0.001GY – 0.67;R2 = 0.996;P ≤ 0.001) and minor (WUEGY = 0.002GY + 0.289;R2 = 0.992;P ≤ 0.001) cropping seasons for all the maize cultivars. The linear models developed for the maize cultivars, re-lating WUEGY to GY, are specific to each of the crop growing seasons, indicating that seasonal rainfall impacts significantly on harvest index of the maize cultivars but differently in each of the crop growing seasons as a results of dif-ferences in seasonal rainfall. However, the models could be used to estimate water use efficiencies of each of the three maize cultivars given the appropriate TDM and GY as inputs for the environment under which the study was conducted.

关键词： Water Use Efficiency Maize Cultivars Rain-Fed

Development of mouse preimplantation embryos in space

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National Science Review 2020年第9期7卷 1437-1446页

作者： Xiaohua Lei Yujing Cao Baohua Ma Yunfang Zhang Lina Ning Jingjing Qian Liwen Zhang Yongcun Qu Tao Zhang Dehong Li Qi Chen Junchao Shi Xudong Zhang Chiyuan Ma Ying Zhang Enkui Duan State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of Sciences University of Chinese Academy of Sciences Shanghai Institute of Technical Physics Chinese Academy of Sciences division of Ionizing Radiation National Institute of Metrology College of Veterinary Medicine Northwest A&F University/Key Laboratory of Animal BiotechnologyMinistry of Agriculture

The development of life beyond planet Earth is a long-standing quest of the human race, but whether normal mammalian embryonic development can occur in space is still unclear. Here, we show unequivocally that preimplantation mouse embryos can develop in space, but the rate of blastocyst formation and blastocyst quality are compromised. Additionally, the cells in the embryo contain severe DNA damage, while the genome of the blastocysts developed in space is globally hypomethylated with a unique set of differentially methylated regions. The developmental defects, DNA damage and epigenetic abnormalities can be largely mimicked by the treatment with ground-based low-dose radiation. However,the exposure to simulated microgravity alone does not cause major disruptions of embryonic development,indicating that radiation is the main cause for the developmental defects. This work advances the understanding of embryonic development in space and reveals long-term extreme low-dose radiation as a hazardous factor for mammalian reproduction.

关键词： space-flight radiation microgravity preimplantation development DNA damage DNA methylation

Effects of December 2014 Great Flood on the Physico-Chemical Properties of the Soils in the Kelantan Plains, Malaysia

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Journal of Water Resource and Protection 2016年第2期8卷 263-276页

作者： Jusop Shamshuddin Qurban Ali Panhwar Radziah Othman Roslan Ismail Hamdan Jol Melissa Alina Yusoff Department of Land Management Faculty of Agriculture Universiti Putra Malaysia Serdang Malaysia Soil Science division Nuclear Institute of Agriculture Tandojam Pakistan

Heavy rain falling on land at the upper reaches of the Kelantan River, Malaysia, on December 2014, had resulted in severe soil erosion and untold damages to croplands. The lower reaches of the river were heavily silted with infertile materials considered unfit for crop production. A study was conducted to explain why the flood phenomenon occurred, to determine the physico-chemical properties of the sediments silted in the Kelantan Plains and to propose measures for soil mitigation. Results showed that the silted sediments were characterized by the presence of quarts, mica, feldspars, kaolinite, gibbsite and hematite believed to come from the top- and subsoil of the upland areas. The sediments’ pH was very low and Al and/or Fe contents were very high, while nitrogen and carbon contents varied from area to area. Soils in the Kelantan Plains badly affected by this great flood needed to undergo proper ameliorative program. The most appropriate measure would be to apply ground magnesium limestone in combination with bio-fertilizer fortified with beneficial microbes that would increase their pH to a level above 5, which consequently eliminates Al3+ and/or Fe2+ that causes toxicity to the crops growing on them. The organic material so added would enhance the formation of soil structures. It is advised that the farming communities in the upper reaches of the Kelantan River would have to follow the advice advocated by the Department of agriculture, Peninsular Malaysia, via MyGAP initiative, in order to sustain agricultural production on their land.

关键词： Crop Production Great Flood Heavy Rainfall Silted Sediments Soil Properties

Genomic insights into the genetic basis of cotton breeding in China

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Molecular Plant 2023年第4期16卷 662-677页

作者： Yiqian Li Zhanfeng Si Guoping Wang Zhuolin Shi Jinwen Chen Guoan Qi Shangkun Jin Zegang Han Wenhao Gao Yue Tian Yun Mao Lei Fang Yan Hu Hong Chen Xiefei Zhu Tianzhen Zhang zhejiang Provincial Key Laboratory of Crop Genetic Resources the Advanced Seed InstitutePlant Precision Breeding AcademyCollege of Agriculture and BiotechnologyZhejiang UniversityHangzhouChina Xinjang Production and Construction Corps Seventh division Agricultural Research Institute KuintunChina state Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricutural UniversityNanjingChina College of biotechnology Jiangsu University of Science and TechnologyZhenjiangChina Key Laboratory of China Northwestern Inland Region Ministry of AgricultureCotton Research InstituteXinjiang Academy of Agricultural and Reclamation ScienceShiheziChina

The excellent Upland cotton(Gossypium hirsutum)cultivars developed since 1949 have made a huge contribution to cotton production in China,the world's largest producer and consumer of ***,the genetic and genomic basis for the improvements of these cotton cultivars remains largely *** this study,we selected 16 Upland cotton cultivars with important historical status in Chinese cotton breeding and constructed a multiparent,advanced generation,intercross(MAGiC)population comprising 920 recombinant inbred lines.A genome-wide association study using the MAGIC population identified 54 genomic loci associated with lint yield and fiber *** them,25(46.30%)pleiotropic genomic loci cause simultaneous changes of lint yield and/or fiber quality traits,revealing complex trade-offs and linkage drags in Upland cotton agronomic *** sequencing data of 11 introduced ancestor cultivars and publicly available resequencing datasets of 839 cultivars developed in China during the past 70 years were integrated to explore the historical distribution and origin of the elite or selected ***,85%oftheseelitealleles were selectedandfixed fromdifferent Americanancestors,consistentwithcotton breeding practices in ***,seven elite alleles of native origin that are responsible for Fusarium wilt resistance,early maturing,good-quality fiber,and other characteristics were not found in American an-cestors but have greatly contributed to Chinese cotton breeding and wide *** together,these results provide a genetic basis for further improving cotton cultivars and reveal that the genetic composition of Chinese cotton cultivars is narrow and mainly derived from early introduced American varieties.

关键词： cotton MAGiC population GWAS whole-genome sequencing pleiotropic loci breeding history

Gene expression of CCL8 and CXCL10 in peripheral blood leukocytes during early pregnancy in cows

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Journal of Animal Science and biotechnology 2018年第4期9卷 813-823页

作者： Ryosuke Sakumoto Kosuke Iga Ken-Go Hayashi Shiori Fujii Hiroko Kanahara Misa Hosoe Tadashi Furusawa division of Animal Breeding and Reproduction Research Institute of Livestock and Grassland Science National Agriculture and Food Research Organization (NARO) division of Livestock and Forage Research Tohoku Agricultural Research CenterNARO division of Animal Sciences Institute of Agrobiological SciencesNARO

Background: The aim of the present study was to evaluate CCL8 and CXCL10 expression and its regulatory mechanism in peripheral blood leukocytes(PBLs) at the time of maternal recognition in cows. Blood samples were collected on 14, 15, 16, 17 and 18 d after artificial insemination(AI). Based on the day of return of estrus, cows were divided into three groups, pregnant(n = 5), early embryonic mortality(EEM; n = 5) and late embryonic mortality(LEM; n = 5). The gene expression levels in PBLs were assessed with quantitative real-time reverse transcription ***: The expression of CCL8 and CXCL10 mRNA in PBLs gradually increased from 14 to 18 d of pregnant cows and significant differences were observed on 18 d(P < 0.05), whereas no significant changes were observed both in EEM and LEM cows. Interferon-stimulated protein 15 k Da(ISG15), myxovirus-resistance gene(MX) 1 and MX2 mRNA expression in PBLs increased from 14 to 18 d which was significant on 18 d of pregnant cows as well as in LEM cows(P < 0.05), but no changes were observed in EEM cows. To determine whether the expression of CCL8 and CXCL10 in PBLs was regulated by pregnancy-related substances or not, expression level was assessed after exposure to interferon-τ(IFNT) and CCL16. Monocytes, granulocytes and lymphocytes were obtained using density-gradient centrifugation and flow cytometry. The addition of IFNT(100 ng/mL) and CCL16(100 ng/mL) to cultured PBLs increased the expression of CCL8 and CXCL10 mRNA(P < 0.05). The expression of ISG15, MX1 and MX2 mRNA in PBLs was also stimulated by IFNT and CCL16(P < 0.05).Conclusions: The expression of CCL8 and CXCL10 genes increased in PBLs during early pregnancy. Since IFNT stimulated CCL8 and CXCL10 expression in cultured PBLs, the increase of CCL8 and CXCL10 might be pregnancy-dependent *** expression of both CCL8 and CXCL10 in PBLs was stimulated by CCL16 as wel as IFNT, suggesting a chemokine interaction that at least includes CCL8, CXCL10 and CCL16, and m

关键词： CCL8 Cow CXCL10 Leukocytes Pregnancy

Life cycle greenhouse gas emissions from five contrasting rice production systems in the tropics

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Pedosphere 2023年第6期33卷 960-971页

作者： Pradeep K.DASH Pratap BHATTACHARYYA Soumya R.PADHY Amaresh K.NAYAK Annie POONAM Sangita MOHANTY Crop Production division Indian Council of Agriculture Research(ICAR)-National Rice Research Institute(NRRI)GuttackOdisha 753006India

Carbon footprint (CF) quantification of major rice production systems (RPSs) is a prerequisite for developing strategies for climate change mitigation in agriculture. Total life cycle greenhouse gas emissions (LC-GHGs) from rice production to consumption might provide precise CFs for RPSs. Therefore, we assessed three segments (pre-farm, on-farm, and post-farm) of LC-GHGs under five major contrasting RPSs, i.e., aerobic rice (AR), shallow lowland rice(SLR), system of rice intensification (SRI), deep water rice (DWR), and zero-tilled direct-seeded rice (ZTR), in India to determine the corresponding *** footprint was the lowest for ZTR, while LC-GHGs were the lowest for AR. Therefore, AR is an adequate option for short-term reduction of GHG emissions. However, ZTR might be promoted by incentives as a long-term strategy. Among segmental LC-GHGs, on-farm GHG emissions contributed less than the other two segmental GHG emissions. The post-farm (i.e., farm gate to consumption) segment contributed the largest proportion (54%–69%) of total LC-GHGs, followed by pre-farm (i.e., cradle to farm) segment (21%–27%) and on-farm operation (11%–23%). These findings suggest that post-farm components that contribute to maximum GHG emissions must be scientifically tackled with proactive policy initiatives. However, the data of this segment are limited and scattered. Therefore, real-time assessment of GHG emissions during post-farm operation and input transportation from cradle to farm requires more precise quantification. Although CF in SRI was higher, this system had the potential to achieve higher yields and better soil carbon storage. Therefore,SRI may be encouraged from the perspectives of food security and long-term sustainability by reducing GHG emissions by three to four times.

关键词： carbon-equivalent emission carbon footprint climate change mitigation global warming management practice soil carbon stock:

Antioxidant and anti-inflammatory roles of tea polyphenols in inflammatory bowel diseases

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Food Science and Human Wellness 2022年第3期11卷 502-511页

作者： Van-Long Truong Woo-Sik Jeong Food and Bio-industry Research Institute School of Food Science&BiotechnologyCollege of Agriculture and Life SciencesKyungpook National UniversityDaegu 41566Korea

Polyphenols,including phenolic acids,flavonoids,and procyanidins,are abundant in food and beverage derived from ***(Camellia sinensis)is particularly rich in polyphenols(e.g.,catechins,theaflavins,thearubigins,gallic acid,and flavonols),which are thought to contribute to the health benefits of *** intake of tea polyphenols has been described to prevent and/or attenuate a variety of chronic pathological conditions like cardiovascular diseases,neurodegenerative diseases,diabetes,and *** review focuses on established antioxidant and anti-inflammatory properties of tea polyphenols and underlying mechanisms of their involvement in inflammatory bowel diseases(IBD).Tea polyphenols act as efficient antioxidants by inducing an endogenous antioxidant defense system and maintaining intracellular redox *** polyphenols also regulate signaling pathways such as nuclear factor-κB,activator protein 1,signal transducer and activator of transcriptions,and nuclear factor E2-related factor 2,which are associated with IBD *** pieces of evidence have indicated that tea polyphenols enhance epithelial barrier function and improve gut microbial dysbiosis,contributing to the management of inflammatory ***,this study suggests that supplementation of tea polyphenols could prevent inflammatory conditions and improve the outcome of patients with IBD.

关键词： Antioxidant Anti-inflammation Epithelial barrier function Inflammatory bowel diseases Gut microbiota Tea polyphenols

Anatomical and Chemical Characteristics of a Rolling Leaf Mutant of Rice and Its Ecophysiological Properties

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Rice science 2008年第3期15卷 201-208页

作者： BAI Lei DUAN Zhuang-qin WANG Jun-min AN Li-zhe ZHAO Zhi-guang CHEN Kun-ming College of Life Sciences Lanzhou University Lanzhou 730000 China Institute of Crop Sciences College of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 China Institute of Crop and nuclear Technology Utilization Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China

The anatomical and chemical characteristics of a rolling leaf mutant （rlm） of rice （Oryza sativa L.） and its ecophysiological properties in photosynthesis and apoplastic transport were investigated. Compared with the wild type （WT）, the areas of whole vascular bundles and xylem as well as the ratios of xylem area/whole vascular bundles area and xylem area/phloem area were higher in rim, whereas the area and the width of foliar bulliform cell were lower. The Fourier transform infrared （FTIR） microspectroscopy spectra of foliar cell walls differed greatly between rim and WT. The rim exhibited lower protein and polysaccharide contents of foliar cell walls. An obvious reduction of pectin content was also found in rim by biochemical measurements. Moreover, the rate of photosynthesis was depressed while the conductance of stoma and the intercellular CO2 concentration were enhanced in rim. The PTS fluorescence, which represents the ability of apoplastic transport, was 11% higher in rim than in WT. These results suggest that the changes in anatomical and chemical characteristics of foliar vascular bundles, such as the reduction of proteins, pectins, and other polysaccharides of foliar cell walls, participate in the leaf rolling mutation, and consequently lead to the reduced photosynthetic dynamics and apoplastic transport ability in the mutant.

关键词： rice rolling leaf mutant anatomical characteristics chemical characteristics apoplastic transport ecophysiological property Fourier transform infrared microspectroscopy