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Journal of Integrative Agriculture 2023年第3期22卷 881-896页

作者： ZHAO Yi-ran ZHAO Yu-zhong LIU Si-dang XIAO Yi-hong LI Ning LIU Kui-hao MENG Fan-liang ZHAO Jun LIU Meng-da LI Bao-quan College of animal Science and Veterinary Medicine Shandong Agricultural University/Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and PreventionShandong Agricultural UniversityTai’an 271018P.R.China Division of Zoonoses Surveillance China Animal Health and Epidemiology CenterQingdao 266033P.R.China

H9N2 avian influenza virus(AIV) has widely circulated in poultry worldwide and sporadic infections in humans and mammals. During our surveillance of chicken from 2019 to 2021 in Shandong Province, China, we isolated 11 H9N2AIVs. Phylogenetic analyses showed that the eight gene segments of the 11 isolates were closely related to several sublineages of Eurasian lineage: BJ/94-like clades(HA and NA genes), G1-like clades(PB2 and M genes), and SH/F/98-like clades(PB1, PA, NP and NS genes). The isolates showed mutation sites that preferentially bind to humanlike receptors(HA) and mammalian fitness sites(PB2, PB1 and PA), as well as mutations in antigen and drug resistance sites. Moreover, studies with mice revealed four isolates with varying levels of pathogenicity. The average antibody titer of the H9N2 AIVs was 8.60 log2. Based on our results, the epidemiological surveillance of H9N2 AIVs should be strengthened.

关键词： influenza virus phylogenetic analyses mutation sites pathogenicity antibody titer epidemiological surveillance

Stress and immunological response of heifers divergently ranked for residual feed intake following an adrenocorticotropic hormone challenge

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Journal of animal Science and Biotechnology 2018年第1期9卷 111-118页

作者： A.K.Kelly P.Lawrence B.Earley D.A.Kenny M.McGee School of Agriculture and Food Science College of Health and Agricultural SciencesUniversity College Dublin Livestock Systems Research Department Teagasc Animal&Grassland Research and Innovation CentreGrangeDunsanyCo. animal and Bioscience Research Department Animal&Grassland Research and Innovation CentreTeagascDunsanyCo.

Background： When an animal is exposed to a stressor, metabolic rate, energy consumption and utilisation increase primarily through activation of the hypothalamic-pituitary-adrenal（HPA） axis. Changes to partitioning of energy by an animal are likely to influence the efficiency with which it is utilised. Therefore, this study aimed to determine the physiological stress response to an exogenous adrenocorticotropic hormone（ACTH） challenge in beef heifers divergently ranked on phenotypic residual feed intake（RFI）.Results： Data were collected on 34 Simmental weaning beef heifers the progeny of a well characterized and divergently bred RFI suckler beef herd. Residual feed intake was determined on each animal during the postweaning stage over a 91-day feed intake measurement period during which they were individually offered adlibitum grass silage and 2 kg of concentrate per head once daily. The 12 highest [0.34 kg DM/d] and 12 lowest[-0.48 kg DM/d] ranking animals on RFI were selected for use in this study. For the physiological stress challenge heifers（mean age 605 ± 13 d; mean BW 518 ± 31.4 kg） were fitted aseptically with indwelling jugular catheters to facilitate intensive blood collection. The response of the adrenal cortex to a standardised dose of ACTH（1.98 IU/kg metabolic BW^（0.75）） was examined. Serial blood samples were analysed for plasma cortisol, ACTH and haematology variables. Heifers differing in RFI did not differ（P = 0.59） in ACTH concentrations. Concentration of ACTH peaked（P 〈 0.001） in both RFI groups at 20 min post-ACTH administration, following which concentration declined to baseline levels by 150 min. Similarly, cortisol systemic profile peaked at 60 min and concentrations remained continuously elevated for 150 min. A RFI × time interaction was detected for cortisol concentrations（P = 0.06） with high RFI heifers had a greater cortisol response than Low RFI from 40 min to 150 min relative to ACTH administration. Cortisol response was positively assoc

关键词： Beef cattle Cortisol Feed efficiency Residual feed intake Stress response

Hydroxyproline alleviates 4-hydroxy-2-nonenal-induced DNA damage and apoptosis in porcine intestinal epithelial cells

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animal Nutrition 2022年第2期9卷 7-15页

作者： Yun Ji Yu He Ying Yang Zhaolai Dai Zhenlong Wu State Key Laboratory of animal Nutrition Department of Animal Nutrition and Feed ScienceChina Agricultural UniversityBeijing 100193China Beijing Advanced Innovation Center for Food Nutrition and Human health China Agricultural UniversityBeijing 100193China

Oxidative stress has been confirmed in relation to intestinal mucosa damage and multiple bowel *** (Hyp) is an imino acid abundant in sow's milk. Compelling evidence has beengathered showing the potential antioxidative properties of Hyp. However, the role and mechanism ofHyp in porcine intestinal epithelial cells in response to oxidative stress remains unknown. In this study,small intestinal epithelial cell lines of piglets (IPEC-1) were used to evaluate the protective effects of Hypon 4-hydroxy-2-nonenal (4-HNE)-induced oxidative DNA damage and apoptosis. IPEC-1 pretreated with0.5 to 5 mmol/L Hyp were exposed to 4-HNE (40 mmol/L) in the presence or absence of Hyp. Thereafter,the cells were subjected to apoptosis detection by Hoechst staining, flow cytometry, and Western blot orDNA damage analysis by comet assay, immunofluorescence, and reverse-transcription quantitative PCR(RT-qPCR). Cell apoptosis and the upregulation of cleaved-caspase-3 induced by 4-HNE (40 mmol/L) wereinhibited by 5 mmol/L of Hyp. In addition, 5 mmol/L Hyp attenuated 4-HNE-induced reactive oxygenspecies (ROS) accumulation, glutathione (GSH) deprivation and DNA damage. The elevation in transcriptionof GADD45a (growth arrest and DNA-damage-inducible protein 45 alpha) and GADD45b(growth arrest and DNA-damage-inducible protein 45 beta), as well as the phosphorylation of H2AX(H2A histone family, member X), p38 MAPK (mitogen-activated protein kinase), and JNK (c-Jun N-terminalkinase) in cells treated with 4-HNE were alleviated by 5 mmol/L Hyp. Furthermore, Hyp supplementationincreased the protein abundance of Krüppel like factor 4 (KLF4) in cells exposed to *** of KLF4 expression by kenpaulone impeded the resistance of Hyp-treated cells to DNAdamage and apoptosis induced by 4-HNE. Collectively, our results indicated that Hyp serves to protectagainst 4-HNE-induced apoptosis and DNA damage in IPEC-1 cells, which is partially pertinent with theenhanced expression of KLF

关键词： Hydroxyproline Apoptosis DNA damage 4-Hydroxy-2-nonenal Oxidative stress

Detection and sequencing of plasmid encoded tetracycline resistance determinants(tetA and tetB) from food-borne Bacillus cereus isolates

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Asian Pacific Journal of Tropical Medicine 2012年第9期5卷 709-712页

作者： Mudasir Ali Rather Rabinder Singh Aulakh Jatinder Paul Singh Gill Abdul Qayoom Mir Mir Nadeem Hassan Division of Veterinary Public health Faculty of Veterinary Sciences and Animal HusbandrySKUAST-KJ&K(190 006)India Department of Veterinary Public health Guru Angad Dev Veterinary and Animal Sciences University-Ludhiana(141 004)PunjabIndia

Objective:To investigate the detection and sequencing of plasmid encoded tetracycline resistance genes(tet A and tet B) from food-borne and standard strains of Bacillus cereus(B. cereus).Methods:PCR was carried out to detect the tetracycline resistance genes(tet A and tet B) in food-borne *** strains and the amplified products were ***:The phenotypic resistance against tetracycline was observed in 39 of the 118 food-borne isolates and two reference strains(MTCC 430 and MTCC 1307) of *** the phenotypically resistant isolates,tet A was detected in 36 food-borne isolates and two reference strains(MTCC 430 and MTCC 1307).whereas,tel B was detected in 12 food-bome isolates and MTCC 1307 strain. Conclusions:A close association was therefore found between phenotypic resistance against tetracycline and presence of tetracycline resistance *** tet A and tet B gene fragments were amplified,purified and *** gene sequences of the isolates studied herein were found similar to tetA and tetB gene sequences of other bacteria available in *** occurrence of tetA and tetB genes in *** indicate the horizontal transfer of antibiotic resistance determinants from other bacteria into *** transfer of these resistant determinants to other potentially pathogenic bacteria may be a matter of great concern.

关键词： Bacillus cereus Tetracycline resistance tetA tetB Sequencing

The Distribution of Different Clades of Seneca Valley Viruses in Guangdong Province,China

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Virologica Sinica 2018年第5期33卷 394-401页

作者： Pan Chen Fan Yang Weijun Cao Huanan Liu Keshan Zhang Xiangtao Liu Zhiwen Xu Zixiang Zhu Haixue Zheng State Key Laboratory of Veterinary Etiological Biology National Foot and Mouth Diseases Reference LaboratoryKey Laboratory of Animal Virology of Ministry of Agriculture Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences animal Biotechnology Center Key Laboratory of Animal Disease and Human Health of Sichuan Province Sichuan Agricultural University

Seneca Valley virus (SVV), a newly determined etiological agent of vesicular disease in swine, causes porcine idiopathic disease and occasional acute death in piglets. Recently, an increased number of SVV infection cases have been reported in the United States (US) and China, resulting in significant economic losses to the swine industry. The first identification of SVV in China was reported in Guangdong Province, a major swine producing province. The cases of SVV were continuously reported in Guangdong in 2015 and 2016. However, the spread of SVV in Guangdong in 2017 remains *** this study, we determined two new SVV strains, CH-GD-2017-1 and CH-GD-2017-2, from Guangdong. The genetic analysis suggested that the two Guangdong strains showed different characteristics to previous Guangdong strains. They showed lower nucleotide similarity with strains isolated in 2015 and 2016, and were more similar to the US *** analyses indicated that the new strains were clustered in a different clade with previous Guangdong *** found 28 mutated amino acids in the new strains, compared with the first Guangdong strain, SVV CH-01-2015. In the geographic analysis, we found that the US and China reported more SVV cases than other countries, and most of the SVV cases were reported in east and central China—of which, Guangdong Province is one of the major epidemic regions. In conclusion, our findings indicate that SVV continued to spread in Guangdong Province in 2017, and two different clades of SVVs have emerged in this region.

关键词： Seneca Valley virus (SVV) Genomic sequence Phylogenetic tree Epidemiological analysis

Role of copper chelating agents: between old applications and new perspectives in neuroscience

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Neural Regeneration Research 2025年第3期20卷 751-762页

作者： Rosalba Leuci Leonardo Brunetti Vincenzo Tufarelli Marco Cerini Marco Paparella Nikola Puvača Luca Piemontese Department of Pharmacy-Pharmaceutical Science University of Bari Aldo MoroBariItaly Department of Precision and Regenerative Medicine and Jonian Area(DiMePRe-J) Section of Veterinary Science and Animal ProductionUniversity of Bari Aldo MoroBariItaly Department of Engineering Management in Biotechnology Faculty of Economics and Engineering Management in Novi SadUniversity Business Academy in Novi SadNovi SadSerbia

The role of copper element has been an increasingly relevant topic in recent years in the fields of human and animal health, for both the study of new drugs and innovative food and feed supplements. This metal plays an important role in the central nervous system, where it is associated with glutamatergic signaling, and it is widely involved in inflammatory processes. Thus, diseases involving copper(Ⅱ) dyshomeostasis often have neurological symptoms, as exemplified by Alzheimer's and other diseases(such as Parkinson's and Wilson's diseases). Moreover, imbalanced copper ion concentrations have also been associated with diabetes and certain types of cancer, including glioma. In this paper, we propose a comprehensive overview of recent results that show the importance of these metal ions in several pathologies, mainly Alzheimer's disease, through the lens of the development and use of copper chelators as research compounds and potential therapeutics if included in multi-target hybrid drugs. Seeing how copper homeostasis is important for the well-being of animals as well as humans, we shortly describe the state of the art regarding the effects of copper and its chelators in agriculture, livestock rearing, and aquaculture, as ingredients for the formulation of feed supplements as well as to prevent the effects of pollution on animal productions.

关键词： agriculture Alzheimer's disease chelators copper feed supplements multi-target

A gene expression estimator of intramuscular fat percentage for use in both cattle and sheep

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Journal of animal Science and Biotechnology 2014年第4期5卷 379-390页

作者： Bing Guo Kritaya Kongsuwan Paul L Greenwood Guanghong Zhou Wangang Zhang Brian P Dalrymple Key Laboratory of Meat Processing and Quality Control Synergetic Innovation Center of Food Safety and NutritionCollege of Food Science and TechnologyNanjing Agriculture University CSIRO animal Food and Health SciencesSt.Lucia QLD 4067Australia National Institute of animal health 50/2 KasetklangLadyaoBangkok 10900Thailand CSIRO animal Food and Health SciencesArmidale NSW 2350Australia NSW Department of primary Industries Armidale NSW 2350Australia

Background：The expression of genes encoding proteins involved in triacyglyceride and fatty acid synthesis and storage in cattle muscle are correlated with intramuscular fat（IMF）%.Are the same genes also correlated with IMF%in sheep muscle,and can the same set of genes be used to estimate IMF%in both species？Results：The correlation between gene expression（microarray） and IMF%in the longissimus muscle（LM） of twenty sheep was *** integrated analysis of this dataset with an equivalent cattle correlation dataset and a cattle differential expression dataset was undertaken.A total of 30 genes were identified to be strongly correlated with IMF%in both cattle and *** overlap of genes was highly significant,8 of the 13 genes in the TAG gene set and 8 of the 13 genes in the FA gene set were in the top 100 and 500 genes respertively most correlated with IMF%in sheep,P-value = *** the 30 genes,CIDEA,THRSP,ACSM1,DGAT2 and FABP4 had the highest average rank in both *** the data from two small groups of Brahman cattle（control and Hormone growth promotant-treated[known to decrease IMF%in muscle]） and 22 animals in total,the utility of a direct measure and different estimators of IMF%（ultrasound and gene expression） to differentiate between the two groups were *** measured IMF%and IMF%estimated from ultrasound scanning could not discriminate between the two ***,using gene expression to estimate IMF%discriminated between the two *** the number of genes used to estimate IMF%from one to five significantly increased the discrimination power;but increasing the number of genes to 15 resulted in little further ***：We have demonstrated the utility of a comparative approach to identify robust estimators of IMF%in the LM in cattle and *** have also demonstrated a number of approaches（potentially applicable to much smaller groups of animals than conventional methods） to using gene expression to rank animal

关键词： Cattle Gene expression phenotype IMF% Sheep

Ablation of NTPDase2+ cells inhibits the formation of filiform papillae in tongue tip

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animal Models and Experimental Medicine 2018年第2期1卷 143-151页

作者： Feng Li Bo-Wen Niu Meng-Min Zhu Department of Laboratory animal Science Shanghai Public Health Clinical CenterFudan University

Background: Lingual epithelia in the tongue tip are among the most rapidly regenerating tissues, but the mechanism of cell genesis in this tissue is still unknown. Previous study has suggested the existence of multiple stem cell pools in lingual epithelia and papillae. Like K14+ and Sox2+ cells, NTPDase2+ cells have characteristics of stem ***: We employed a system using doxycycline to conditionally ablate NTPDase2+ cells in lingual epithelia and papillae by regulated expression of the diphtheria toxin A(DTA) gene. Transgenic lines, which expressed the rtTA gene in NTPDase2+ cells, were produced by pronuclear injection of zygotes from C57 BL/6 mice using the BAC clone RP23-47 P18. The NTPDase2-rtTA transgenic mice were crossed with the TetO-DTA transgenic animals. The double transgenic mice were treated with doxycycline. Doxycycline(Dox) was diluted in 5% sucrose in water to a final concentration of 0.3-0.5 mg/mL and supplied as drinking ***: After 15 days of Dox induction, the expression of NTPDase2, Sox2 and K14 was ablated from lingual epithelia. DTA expression in NTPDase2+cells did not inhibit the turnover of GNAT3+ or PLCb2+ cells in taste buds,nor the expression of S100 b beneath lingual epithelia and papillae. After35 days ablation of NTPDase2+ cells, the basic structure of lingual epithelia and papillae remained intact. However, the ratio of cell to total tissue area was decreased in lingual epithelia and circumvallate(CV) papillae. DTA expression also inhibited the regeneration of filiform papillae on the dorsal surface of the tongue ***: These studies provide important insights into the understanding of dynamic equilibrium among the multiple stem cell populations present in the lingual epithelia and papillae.

关键词： cell ablation lingual epithelia NTPDase2 papillae tissue regeneration

Advances in fatty acids nutrition in dairy cows: from gut to cells and effects on performance

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Journal of animal Science and Biotechnology 2021年第1期12卷 1-36页

作者： Massimo Bionaz Einar Vargas-Bello-Pérez Sebastiano Busato Department of animal and Rangeland Sciences Oregon State UniversityCorvallisOR 97331USA Department of Veterinary and animal Sciences Faculty of Health and Medical SciencesUniversity of CopenhagenGrønnegårdsvej 3DK-1870 Frederiksberg CDenmark.

High producing dairy cows generally receive in the diet up to 5–6% of fat. This is a relatively low amount of fat in the diet compared to diets in monogastrics;however, dietary fat is important for dairy cows as demonstrated by the benefits of supplementing cows with various fatty acids(FA). Several FA are highly bioactive, especially by affecting the transcriptome;thus, they have nutrigenomic effects. In the present review, we provide an up-to-date understanding of the utilization of FA by dairy cows including the main processes affecting FA in the rumen,molecular aspects of the absorption of FA by the gut, synthesis, secretion, and utilization of chylomicrons;uptake and metabolism of FA by peripheral tissues, with a main emphasis on the liver, and main transcription factors regulated by FA. Most of the advances in FA utilization by rumen microorganisms and intestinal absorption of FA in dairy cows were made before the end of the last century with little information generated afterwards. However,large advances on the molecular aspects of intestinal absorption and cellular uptake of FA were made on monogastric species in the last 20 years. We provide a model of FA utilization in dairy cows by using information generated in monogastrics and enriching it with data produced in dairy cows. We also reviewed the latest studies on the effects of dietary FA on milk yield, milk fatty acid composition, reproduction, and health in dairy cows. The reviewed data revealed a complex picture with the FA being active in each step of the way, starting from influencing rumen microbiota, regulating intestinal absorption, and affecting cellular uptake and utilization by peripheral tissues, making prediction on in vivo nutrigenomic effects of FA challenging.

关键词： Absorption Dairy cow Dietary fatty acids Intestine Liver Metabolism Performance Transcription factors Transport

Amino acid sensing in the gut and its mediation in gut-brain signal transduction

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animal Nutrition 2016年第2期2卷 69-73页

作者： Jing Liu Kaifan Yu Weiyun Zhu Laboratory of Gastrointestinal Microbiology Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health College of Animal Science and TechnologyNanjing Agricultural University

animal gastrointestinal tract is not only a digestive organ, but also a nutrient sensing organ which detects luminal nutrient and thus can regulate food intake. There are many amino acid sensing receptors and transporters in the gut. Amino acids sensing by these receptors and transporters can stimulate the intestinal endocrine cells to release a variety of gut hormones. These hormones trigger a series of physiological effects via the nerve system. This review summarized the recent advance on the amino acid sensing receptors and transporters in the gastrointestinal tract, the gut hormones released from the intestinal endocrine cells and the hormones-induced signal transduction between the gut and brain. A better understanding of these processes may help to gain further insight into the specific role of amino acids in digestion and provide guidelines in developing strategy for the better use of amino acids in the diet.

关键词： Amino acid sensing Gut hormones Sensing receptor Amino acid transporter Gut-brain signaling