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Long-term clinical effects of intravitreal injections of conbercept for the treatment of choroidal neovascularization in patients with pathological myopia

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International Journal of Ophthalmology(English edition) 2022年第12期15卷 1971-1977页

作者： Si Zhang Zi-Fang He Fei-Fei Chen Wen-Wen Zhang Ya-Jun Liu Hui Chen Zheng-Gao Xie Nanjing Drum Tower Hospital the Affiliated Hospital of Nanjing University Medical SchoolNanjing 210008Jiangsu ProvinceChina

AIM:To observe the long-term clinical efficacy of intravitreal injections of conbercept,a novel vascular growth factor inhibitor,for the treatment of pathological myopia choroidal neovascularization(PM-CNV).METHODS:A total of 67 eyes(from 67 patients;mean age,54.90±12.7y)with PM-CNV were retrospectively *** on the different schemes used for the administration of the drug,the patients were divided into two groups:group A(n=35;average age,53.31±13.6y;average diopter,9.25±1.72 D),which received only one injection of pro re nata(PRN;1+PRN regimen),and group B(n=32;average age,56.49±11.8y;average diopter,9.63±2.24 D),which received one injection per month for 3mo(3+PRN regimen).Best-corrected visual acuity(BCVA)analysis,intraocular pressure(IOP)examination,slit-lamp microscopy,fundus examination and optical coherence tomography were per formed at each *** recurrence and treatment times of CNV were *** patients were followed up for at least ***:The BCVA was increased in 29 eyes(82.9%)in group A and 30 eyes(93.75%)in group B;no increase or decrease was observed in 6(17.1%)and 2(6.25%)eyes in groups A and B,*** BCVA(log MAR)values before treatment(0.67±0.48 and 0.71±0.56)were significantly higher than those 12mo after treatment(0.31±0.26 and 0.33±0.17)in groups A and B,respectively(P<0.05).The mean central macular thickness(CMT)values had significantly decreased from 346.49±65.99 and 360.10±82.31μm at baseline to 257.29±40.47 and 251.97±48.26μm in groups A and B,respectively,after 12mo of treatment.A total of 21 eyes in group A needed reinjection(60%;average number of injections,2.51±0.98);the corresponding values in group B were 6 eyes(18.75%;average number of injections,3.74±1.22).There were no adverse ocular and systemic complications during the treatment and ***:Intravitreal injection of conbercept with 1+PRN or 3+PRN improve the visual acuity,reduce macular edema and reduce the level of CMT in patie

关键词： pathological myopia choroidal neovascularization conbercept best-corrected visual acuity central macular thickness

Engineered macrophage-biomimetic versatile nanoantidotes for inflammation-targeted therapy against Alzheimer’s disease by neurotoxin neutralization and immune recognition suppression

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Bioactive Materials 2023年第8期26卷 337-352页

作者： Meng Cheng Caihua Ye Chunxiao Tian Dongju Zhao Haonan Li Zuhao Sun Yuyang Miao Qiang Zhang Junping Wang Yan Dou Department of Radiology and Tianjin Key Laboratory of Functional Imaging Tianjin Medical University General HospitalTianjin300052PR China school of Biomedical Engineering and Technology Tianjin Medical UniversityTianjin300070PR China school of Life Sciences Tianjin UniversityTianjin300072PR China Department of Geriatrics Tianjin Medical University General HospitalTianjin Geriatrics InstituteTianjin300052PR China

Immune recognition of excessive neurotoxins by microglia is a trigger for the onset of neuroinflammation in the brain,leading to neurodegeneration in Alzheimer’s disease(AD).Blocking active recognition of microglia while removing neurotoxins holds promise for fundamentally alleviating neurotoxin-induced immune responses,but is very ***,an engineered macrophage-biomimetic versatile nanoantidote(OT-Lipo@M)is developed for inflammation-targeted therapy against AD by neurotoxin neutralization and immune recognition *** macrophage membranes can not only endow OT-Lipo@M with anti-phagocytic and inflammation-tropism capabilities to target inflammatory lesions in AD brain,but also efficiently reduce neurotoxin levels to prevent them from activating *** loaded oxytocin(OT)can be slowly released to downregulate the expression of immune recognition site Toll-like receptor 4(TLR4)on microglia,inhibiting TLR4-mediated pro-inflammatory signalling *** from this two-pronged immunosuppressive strategy,OT-Lipo@M exhibits outstanding therapeutic effects on ameliorating cognitive deficits,inhibiting neuronal apoptosis,and enhancing synaptic plasticity in AD mice,accompanied by the delayed hippocampal atrophy and brain microstructural disruption by in vivo 9.4T MR *** work provides new insights into potential AD therapeutics targeting microglia-mediated neuroinflammation at the source.

关键词： Alzheimer’s disease Biomimetic nanomedicine Biological neutralization Immune recognition and response Neuroinflammation-targeted therapy 9.4T MR imaging

Hybrid discrete-continuum model of tumor growth considering capillary points

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Applied Mathematics and Mechanics(English Edition) 2013年第10期34卷 1237-1246页

作者：吕杰许世雄姚伟周瑜龙泉 Department of medical Instrumentation Engineering Shanghai Medical Instrumentation College Department of Mechanics and Engineering Science Fudan University Neusoft medical System Brunel Institute for Bioengineering School of Engineering and DesignBrunel UniversityUxbridge Middlesex UB8 3PH U. K

A hybrid discrete-continuum model of tumor growth in the avascular phase considering capillary points is established. The influence of the position of capillary points on tumor growth is also studied by simulation. The results of the dynamic tumor growth and the distribution of oxygen, matrix-degrading enzymes, and extracellular matrixconcentration in the microenvironment with respect to time are shown by graphs. The relationships between different oxygenated environments and the numbers of surviving, dead, proliferative, and quiescent tumor cells are also investigated.

关键词： mathematical model capillary point tumor growth oxygen

Decoding the brain:Unveiling comprehensive cellular atlases through multiomics mega-data

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The Innovation 2024年第4期5卷 9-10页

作者： Qianwen Wang Wenli Tang Lukui Chen Lin Deng Guangchuang Yu Department of Bioinformatics School of Basic Medical SciencesSouthern Medical UniversityGuangzhou 510515China Department of Neurosurgery Southern Medical University Hosptial of Integrated Traditional Chinese and Western MedicineSouthern Medical UniversityGuangzhou 510515China

The pursuit to comprehend the intricate nature of the brain,an organ of unparalleled complexity,remains a persistent endeavor in the field of neuroscience.1 Recent strides by the National Institutes of Health’s BRAIN Initiative–Cell Census Network(BICCN)have yielded comprehensive cellular atlases for mice and humans,2,3 published in Nature and Science,***,a 3D cell-type atlas of the macaque brain,contributed by Cell,offers profound insights into non-human primate cortical intricacies.4 These collaborative initiatives,driven by the US’s BICCN and China’s Brain Project,represent a significant leap forward in unraveling the cellular intricacies of the brain,culminating in groundbreaking exploration across mice,non-human primates,and *** confluence of these works serves to elucidate the intricate cellular landscapes of the brain through a multiomics approach,signifying a pivotal advancement in neuroscience research toward comprehensive understanding and collaborative exploration(Figure 1).

关键词： comprehensive Figure landscape

Modelling the development of biological structures displaying longitudinal geometries in vitro:culturing pluripotent stem cells on plasma-treated,growth factor-coupled polycaprolactone fibres

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Engineered Regeneration 2024年第1期5卷 124-138页

作者： Badwi B.Boumelhem Stuart T.Fraser Syamak Farajikhah Rachel A.Shparberg Michael B.Morris Marcela M.M.Bilek Anyu Zhang Behnam Akhavan Simon Fleming Maryanne Large school of Physics Faculty of ScienceUniversity of SydneyAustralia Sydney Nano Institute University of SydneyAustralia school of Chemical and Biomolecular Engineering University of SydneyAustralia school of medical Sciences Faculty of Medicine and HealthUniversity of SydneyAustralia school of Biomedical Engineering Faculty of EngineeringUniversity of SydneyAustralia school of Engineering University of NewcastleCallaghanNSW 2308Australia Hunter medical Research Institute(HMRI) Precision Medicine ProgramNew Lambton Heights NSW 2305Australia

Many biological structures such as nerves,blood and lymphatic vessels,and muscle fibres exhibit longitudinal ge-ometries with distinct cell types extending along both the length and width of internal linear *** these three-dimensional structures in vitro is challenging:the best-defined stem-cell differentiation systems are mono-layer cultures or organoids using pluripotent stem *** stem cells can differentiate into functionally mature cells depending on the signals received,holding great promise for regenerative ***,the integration of in vitro differentiated cell types into diseased tissue remains a *** scaffolds can bridge this gap if the appropriate signalling systems are incorporated into the ***,we have taken a biomimicry approach to generate longitudinal structures in *** this approach,mouse embryonic stem cells are directed to differentiate to specific cell types on the surface of polycaprolactone(PCL)fibres treated by plasma-immersion ion implantation and to which with lineage-specifying molecules have been covalently *** demonstrate the simplicity and utility of our method for efficiently generating high yields of the following cell types from these pluripotent stem cells:neurons,vascular endothelial cells,osteoclasts,adipocytes,and cells of the erythroid,myeloid,and lymphoid *** arranged plasma-treated scaffolds with differentiated cell types could ultimately serve as a means for the repair or treatment of diseased or damaged tissue.

关键词： Pluripotent stem cells Lineage commitment Growth factors Biomaterial engineering Plasma-immersion ion implantation Covalent biomolecule attachment Polycaprolactone

Laparoscopic management of totally intra-thoracic stomach with chronic volvulus

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World Journal of Gastroenterology 2013年第35期19卷 5848-5854页

作者： Toygar Toydemir Gkhan Cipe Oguzhan Karatepe Mehmet Ali Yerdel Department of General Surgery Istanbul Surgery Hospital Department of General Surgery Medical School of Bezmialem Vak f University

AIM:To evaluate the outcomes of patients who underwent laparoscopic repair of intra-thoracic gastric volvulus(IGV)and to assess the preoperative ***:A retrospective review of a prospectively collected database of patient medical records identified14 patients who underwent a laparoscopic repair of *** procedure included reduction of the stomach into the abdomen,total sac excision,reinforced hiatoplasty with mesh and construction of a partial *** perioperative data,operative details and complications were *** patients had at least 6 mo of ***:There were 4 male and 10 female *** mean age and the mean body mass index were 66years and 28.7 kg/m2,*** patients presented with epigastric discomfort and early *** was no mortality,and none of the cases were converted to an open *** mean operative time was235 min,and the mean length of hospitalization was 2 *** were no intraoperative *** minor complications occurred in 3 patients including pleuraleffusion,subcutaneous emphysema,dysphagia and delayed gastric *** minor complications resolved spontaneously without any *** the mean follow-up of 29 mo,one patient had a radiological wrap herniation without *** remains symptom free with daily ***:The laparoscopic management of IGV is a safe but technically demanding *** best outcomes can be achieved in centers with extensive experience in minimally invasive esophageal surgery.

关键词： Laparoscopic procedure Hiatal hernia Stomach Volvulus Mesh repair

Promising dawn in tumor microenvironment therapy:engineering oral bacteria

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International Journal of Oral Science 2024年第2期16卷 208-224页

作者： Zifei Wang Wansu Sun Ruixue Hua Yuanyin Wang Yang Li Hengguo Zhang Key Laboratory of Oral Diseases Research of Anhui Province College&Hospital of StomatologyAnhui Medical UniversityHefeiChina Department of Stomatology The First Affiliated Hospital of Anhui Medical UniversityHefeiChina Department of Genetics School of Life ScienceAnhui Medical UniversityHefeiChina

Despite decades of research,cancer continues to be a major global health *** human mouth appears to be a multiplicity of local environments communicating with other organs and causing diseases via ***,the role of oral microbes in the development and progression of cancer has received increasing *** the same time,bioengineering technology and nanotechnology is growing rapidly,in which the physiological activities of natural bacteria are modified to improve the therapeutic efficiency of *** engineered bacteria were transformed to achieve directed genetic reprogramming,selective functional reorganization and precise *** contrast to endotoxins produced by typical genetically modified bacteria,oral flora exhibits favorable biosafety *** outline the current cognitions upon oral microbes,engineered microbes and human cancers,related literatures were searched and reviewed based on the PubMed *** focused on a number of oral microbes and related mechanisms associated with the tumor microenvironment,which involve in cancer occurrence and *** engineering oral bacteria can be a possible application of cancer therapy is worth consideration.A deeper understanding of the relationship between engineered oral bacteria and cancer therapy may enhance our knowledge of tumor pathogenesis thus providing new insights and strategies for cancer prevention and treatment.

关键词： prevention organs precise

The cancer-testis gene,MEIOB,sensitizes triple-negative breast cancer to PARP1 inhibitors by inducing homologous recombination deficiency

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Cancer Biology & Medicine 2021年第1期18卷 74-87页

作者： Yayun Gu Cheng Wang Rongxuan Zhu Jianshui Yang Wenwen Yuan Yanhui Zhu Yan Zhou Na Qin Hongbing Shen Hongxia Ma Hongxia Wang Xiaoan Liu Zhibin Hu State Key Laboratory of Reproductive Medicine Center for Global HealthSchool of Public HealthNanjing Medical UniversityNanjing 211166China Jiangsu Key Lab of Cancer Biomarkers Prevention and TreatmentJiangsu Collaborative Innovation Center for Cancer Personalized MedicineNanjing Medical UniversityNanjing 211116China Department of Bioinformatics School of Biomedical Engineering and InformaticsNanjing Medical UniversityNanjing 211166China Department of Oncology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China Department of Breast Surgery The First Affiliated Hospital with Nanjing Medical UniversityNanjing 210029China

Objective:The newly defined cancer-testis(CT)gene,MEIOB,was previously found to play key roles in DNA double-strand break(DSB)*** this study,we aimed to investigate the effects and mechanisms of MEIOB in the carcinogenesis of triple-negative breast cancers(TNBCs).Methods:The Cancer Genome Atlas database was used to quantify the expression of *** regression analysis was used to evaluate the association between MEIOB expression and the prognosis of human *** effects of MEIOB on cell proliferation and migration in TNBCs were also assessed in ***-derived xenograft(PDX)models were used to assess the sensitivity of breast cancers with active MEIOB to PARP1 ***:We confirmed MEIOB as a CT gene whose expression was restricted to the testes and breast tumors,especially *** activation was significantly associated with poor survival in breast cancer patients[overall,hazard ratio(HR)=1.90(1.16–2.06);TNBCs:HR=7.05(1.16–41.80)].In addition,we found that MEIOB was oncogenic and significantly promoted the proliferation of TNBC *** analysis showed that MEIOB participated in DSB repair in ***,in contrast to its function in meiosis,it mediated homologous recombination deficiency(HRD)through the activation of poly ADP-ribose polymerase(PARP)1 by interacting with ***,activated MEIOB was shown to confer sensitivity to PARP inhibitors,which was confirmed in PDX ***:MEIOB played an oncogenic role in TNBC through its involvement in *** addition,dysregulation of MEIOB sensitized TNBC cells to PARP inhibitors,so MEIOB may be a therapeutic target of PARP1 inhibitors in TNBC.

关键词： Cancer-testis gene MEIOB triple-negative breast cancer PARP1 inhibitor cell proliferation

Influence of expressed TRAIL on biophysical properties of the human leukemic cell line Jurkat

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Cell Research 2004年第2期14卷 161-168页

作者： Kai CHEN~(1,*), Dan LI~(1,*), Yu Hui JIANG~1, Wei Juan YAO~1, Xin Juan WANG~2, Xiao Chao WEI~2, Jing GAO~3, Li De XIE~1, Zong Yi YAN~4, Zong Yao WEN~(1**), Shu CHIEN~5 ~1Hemorheology Center, Department of Biophysics, school of Basic medical Sciences, Peking University, Beijing, 100083, China. ~2Department of Biochemical and Molecular Biology, school of Basic medical Sciences, Peking University, Beijing, 100083, China. ~3Department of Urology, medical Center, New York University, USA. ~4Department of Mechanics and Engineering Science, Peking University, Beijing 100871, China. ~5Department of Bioengineering and Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla, CA 92093-0412, USA Department of Biophysics Hemorheology Center School of Basic Medical Sciences Peking University Beijing China Department of Biochemical and Molecular Biology School of Basic Medical Sciences Peking University Beijing China Department of Urology Medical Center New York University USA Department of Mechanics and Engineering Science Peking University Beijing China Department of Bioengineering and Whitaker Institute of Biomedical Engineering University of California La Jolla USA

The cDNA fragment of human TRAIL (TNF-related apoptosis inducing ligand) was cloned into RevTet-On, a Tetregulated and high-level gene expression system. The gene expression system was constructed in a human leukemic cell line: Jurkat. By using RevTet-On TRAIL gene expression system in Jurkat as a cell model, we studied the influence of TRAIL gene on the changes of cellular apoptosis before and after the TRAIL gene expression, which was induced by adding tetracycline derivative doxycycline (Dox). The results indicated that the cellular apoptosis ratio was largely dependent on the TRAIL gene expression level. Moreover, it was found that the apoptosis-inducing TRAIL could cause significant changes in the biophysical properties of Jurkat cells. The cell surface charge density decreased, the membrane fluidity declined, the elastic coefficients K_I increased, and the proportion of α-helix in membrane protein secondary structure decreased. Thus, the apoptosis-inducing TRAIL gene caused significant changes on

关键词： TRAIL Tet gene expression system Jurkat apoptosis.