检索结果-南通市图书馆

Fatty acid oxidation and autophagy promote endoxifen resistance and counter the effect of AKT inhibition in ER-positive breast cancer cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2021年第6期13卷 433-444页

作者： Lei Duan Sarah Calhoun Daeun Shim Ricardo E.Perez Lothar A.Blatter Carl G.Maki Department of Cell and Molecular Medicine Rush University Medical CenterChicagoIL 60612USA Department of Molecular Biophysics and Physiology Rush University Medical CenterChicagoIL 60612USA

Tamoxifen(TAM)is the first-line endocrine therapy for estrogen receptor-positive(ER+)breast cancer(BC).However,acquired resistance occurs in∼50%***,although the PI3K/AKT/mTOR pathway is a viable target for treatment of endocrine therapy-refractory patients,complex signaling feedback loops exist,which can counter the effectiveness of inhibitors of this ***,we analyzed signaling pathways and metabolism in ER+MCF7 BC cell line and their TAM-resistant derivatives that are co-resistant to endoxifen using immunoblotting,quantitative polymerase chain reaction,and the Agilent Seahorse XF *** found that activation of AKT and the energy-sensing kinase AMPK was increased in TAM and endoxifen-resistant ***,ERRα/PGC-1βand their target genes MCAD and CPT-1 were increased and regulated by AMPK,which coincided with increased fatty acid oxidation(FAO)and autophagy in TAM-resistant *** of AKT feedback-activates AMPK and ERRα/PGC-1β-MCAD/CPT-1 with a consequent increase in FAO and autophagy that counters the therapeutic effect of endoxifen and AKT ***,our results indicate increased activation of AKT and AMPK with metabolic reprogramming and increased autophagy in TAM-resistant *** inhibition of AKT and FAO/autophagy is necessary to fully sensitize resistant cells to endoxifen.

关键词： endoxifen fatty acid oxidation autophagy AMPK AKT

A wake-up call to action for smoking cessation interventions

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Pediatrics 2021年第4期17卷 434-437页

作者： Farahnak Assadi Division of Nephrology Department of PediatricsRush University Medical Center445 East North Water StreetChicagoIL60611USA

Recent data obtained through analysis of the World Health Organization(WHO)on Tobacco epidemic 2019 indicate that about 5 million people(65%)of the world's population are now covered by at least by one MPOWER measure(Monitor tobacco use and prevention policies;protect people from tobacco smoke;offer help to quit tobacco use;warn about the dangers of tobacco;enforce bans on tobacco advertising,promotion and sponsorship;raise taxes on tobacco)at the highest achievement levels,which has been quadrupled since 2007,when one billion people(15%)were covered^([1]).Of the 5 million people protected by at least one complete POWER measure,3.9 billion live in low-and middle-income *** and Turkey are the only two middle-income countries that have adopted all POWER measures at the highest level.

关键词： measures income prevention

Hemoglobin integrated red blood cell membrane-coated metalorganic framework nano-platform for improving the self-adaptive blood glucose management

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2025年第1期18卷 618-627页

作者： Shuai Wu Li Zhu Sheng Ni Xiong Zhao Meng Yan Yuan Zhong Karissa Kusuma Yi Liang Kaiwen Bao Kai Qu Xian Qin Kun Zhang Wuquan Deng Da Sun Wei Wu Key Laboratory of Biorheological Science and Technology Ministry of EducationCollege of BioengineeringChongqing UniversityChongqing 400044China Chongqing University Three Gorges Hospital Chongqing Municipality Clinical Research Center for Endocrinology and Metabolic DiseasesChongqing 404000China Institute of Life Sciences&Biomedical Collaborative Innovation center of Zhejiang Province Wenzhou UniversityWenzhou 325035China Department of Endocrinology School of MedicineChongqing Emergency Medical CenterChongqing University Central HospitalChongqing 400014China

Diabetes,a prevalent chronic metabolic disorder,often leads to severe ***,existing treatment methods may pose life-threatening risks due to poor patient compliance and inaccurate dosing of subcutaneous insulin ***,a biomimetic red blood cell(RBC)membrane-coated glucose-responsive nanoplatform is developed for controlling insulin *** nanoplatforms with RBC membrane can prolong the half-life of nano-formulation in vivo mediated by the biomimetic immune ***,the cascade catalytic effect of glucose oxidase(GOx)encapsulated in metal-organic frameworks(MOFs)and hemoglobin(Hb)in the RBC membrane are able to not only facilitate glucose-responsive insulin release,but also eliminate the detrimental by-product hydrogen peroxide(H_(2)O_(2))resulting from the Hb mediated H_(2)O_(2)*** in vitro and in vivo studies have demonstrated the favorable glucose-responsive performances of this advanced nano-platform with a single intravenous injection maintaining blood glucose balance in Type 1 Diabetes(T1D)mice for an extended duration without the hypoglycemia ***,this biomimetic insulin delivery system is poised to function as a strategy for the intravenous insulin administration,offering a promising drug candidate for the self-adaptive long-term T1D treatment.

关键词： glucose-responsive metal-organic frameworks red blood cell membrane insulin delivery glucose oxidase hemoglobin

Surgery for Peyronie＇s disease

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2013年第1期15卷 27-34页

作者： Laurence A Levine Stephen M Larsen Department of Urology Rush University Medical Center Chicago IL 60612 USA

Peymnie＇s disease （PD） is most simply referred to as a fibrotic wound-healing disorder of the tunica albuginea. It is both a physically and psychologically devastating disorder that causes penile deformity, curvature, hinging, narrowing and shortening, which may compromise sexual function. Although a variety of non-surgical treatments have been suggested, none to date offer a reliable and effective correction of the penile deformity. As a result, surgery remains the gold standard treatment option, offering the most rapid and reliable treatment which will be the focus of this article. We review the preoperative evaluation, surgical algorithm, graft materials and postoperative management of PD. Outcomes for tunical shortening, tunical lengthening and penile prosthesis placement for penile straightening are reviewed. Tunica albuginea plication is the preferred method of straightening for men with adequate rigidity and less severe disease defined as curvature less than 70° without narrowing/hinging. Men who have more severe, complex deformity, but maintain strong preoperative erectile function should be considered candidates for straightening with plaque incision or partial excision and grafting. Finally, for those men who have inadequate rigidity and PD, penile prosthesis placement with straightening is the best approach to address both problems.

关键词： penile prosthesis Peyronie's disease, penile reconstruction plaque excision and grafting plaque incision tunica albuginea plication

An overview of the management of post-vasectomy pain syndrome

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2016年第3期18卷 332-337,I0006页

作者： Wei Phin Tan Laurence A Levine Department of Urology Rush University Medical Center Chicago Illinois USA.

Post-vasectomy pain syndrome remains one of the more challenging urological problems to manage. This can be a frustrating process for both the patient and clinician as there is no well-recognized diagnostic regimen or reliable effective treatment. Many of these patients will end up seeing physicians across many disciplines, further frustrating them. The etiology of post-vasectomy pain syndrome is not clearly delineated. Postulations include damage to the scrotal and spermatic cord nerve structures via inflammatory effects of the immune system, back pressure effects in the obstructed vas and epididymis, vascular stasis, nerve impingement, or perineural fibrosis. Post-vasectomy pain syndrome is defined as at least 3 months of chronic or intermittent scrotal content pain. This article reviews the current understanding of post-vasectomy pain syndrome, theories behind its pathophysiology, evaluation pathways, and treatment options.

关键词： epididymectomy microdenervation orchalgia post-vasectomy pain management post-vasectomy pain syndrome testicular pain vasectomy reversal vaso-vasostomy

Modern treatment strategies for penile prosthetics in Peyronie’s disease:a contemporary clinical review

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2020年第1期22卷 51-59页

作者： Matthew J Ziegelmann M Ryan Farrell Laurence A Levine Division of Urology Rush University Medical CenterChicagoIL 60612USA

Peyronie's disease is a common condition resulting in penile deformity,psychological bother,and sexual *** dysfunction is one common comorbid condition seen in men with Peyronie’s disease,and its presence significantly impacts treatment *** a man with Peyronie's disease and significant erectile dysfunction who desires the most reliable treatment,penile prosthesis placement should be strongly *** some instances,such as those patients with relatively mild curvature,prosthesis placement alone may result in adequate ***,many patients will require additional straightening maneuvers such as manual modeling,penile plication,and tunica albuginea incision with or without *** patients with severe penile shortening,penile length restoration techniques may also be ***,we provide a comprehensive clinical review of penile prosthesis placement in men with Peyronie's ***,we discuss preoperative indications,intraoperative considerations,adjunctive straightening maneuvers,and postoperative outcomes.

关键词： collagenase grafting penile length penile prosthesis Peyronie's

Evolving management of colorectal cancer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2008年第25期14卷 3956-3967页

作者： Jochem van der Voort van Zijp Harald J Hoekstra Marc D Basson Department of Radiation Oncology Leiden University Medical Center Department of Surgery University of Groningen Medical Center John D. Dingell va medical center and Wayne State University

This article reviews recent advances in surgical techniques and adjuvant therapies for colorectal cancer, including total mesorectal excision, the resection of liver and lung metastasis and advances in chemoradiation and foreshadows some interventions that may lie just beyond the frontier. In particular, little is known about the intracellular and extracellular cascades that may influence colorectal cancer cell adhesion and metastasis. Although the phosphorylation of focal adhesion kinases and focal adhesion associated proteins in response to integrin-mediated cell matrix binding ("outside in integrin signaling") is well described, the stimulation of cell adhesion by intracellular signals activated by pressure prior to adhesion represents a different signal paradigm. However, several studies have suggested that increased pressure and shear stress activate cancer cell adhesion. Further studies of the pathways that regulate integrin-driven cancer cell adhesion may identify ways to disrupt these signals or block integrin-mediated adhesion so that adhesion and eventual metastasis can be prevented in the future.

关键词： Adhesion Colorectal Signaling Cancer Surgery

Conditionally Reprogrammed Human Normal Airway Epithelial Cells at ALI: A Physiological Model for Emerging Viruses

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Virologica Sinica 2020年第3期35卷 280-289页

作者： Xuefeng Liu Yuntao Wu Lijun Rong Department of Pathology Center for Cell ReprogrammingGeorgetown University Medical CenterWashingtonDCUSA Department of Oncology Lombardi Comprehensive Cancer CenterGeorgetown University Medical CenterWashingtonDCUSA National center for Biodefense and Infectious Diseases School of Systems BiologyGeorge Mason UniversityManassasVA 20110USA Department of Microbiology and Immunology University of Illinoi at ChicagoChicagoIL 60612USA

Cancer cell lines have been used widely in cancer biology, and as biological or functional cell systems in many biomedical research fields. These cells are usually defective for many normal activities or functions due to significant genetic and epigenetic changes. Normal primary cell yields and viability from any original tissue specimens are usually relatively low or highly variable. These normal cells cease after a few passages or population doublings due to very limited proliferative capacity. Animal models(ferret, mouse, etc.) are often used to study virus-host interaction. However, viruses usually need to be adapted to the animals by several passages due to tropism restrictions including viral receptors and intracellular restrictions. Here we summarize applications of conditionally reprogrammed cells(CRCs), long-term cultures of normal airway epithelial cells from human nose to lung generated by conditional cell reprogramming(CR) technology, as an ex vivo model in studies of emerging viruses. CR allows to robustly propagate cells from non-invasive or minimally invasive specimens, for example, nasal or endobronchial brushing. This process is rapid(2 days) and conditional. The CRCs maintain their differentiation potential and lineage functions, and have been used for studies of adenovirus, rhinovirus, respiratory syncytial virus, influenza viruses, parvovirus, and SARS-CoV. The CRCs can be easily used for airliquid interface(ALI) polarized 3 D cultures, and these coupled CRC/ALI cultures mimic physiological conditions and are suitable for studies of viral entry including receptor binding and internalization, innate immune responses, viral replications, and drug discovery as an ex vivo model for emerging viruses.

关键词： Normal cells Cell senescence Conditional reprogramming Physiological conditions Functional models Air-liquid interface(ALI) Emerging viruses SARS-CoVs

Melanoma:Molecular genetics,metastasis,targeted therapies,immunotherapies,and therapeutic resistance

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genes & Diseases 2022年第6期9卷 1608-1623页

作者： William Wagstaff Rimel N.Mwamba Karina Grullon Mikhayla Armstrong Piao Zhao Bryce Hendren-Santiago Kevin H.Qin Alexander J.Li Daniel A.Hu Andrew Youssef Russell R.Reid Hue H.Luu Le Shen Tong-Chuan He Rex C.Haydon The Pritzker School of Medicine and the Medical Scientist Training ProgramThe University of Chicago Medical CenterChicagoIL 60637USA Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA Department of Orthopaedic Surgery The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016PR China Laboratory of Craniofacial Suture Biology and Development Department of Surgery Section of Plastic SurgeryThe University of Chicago Medical CenterChicagoIL 60637USA Department of Surgery The University of Chicago Medical CenterChicagoIL 60637USA

Cutaneous melanoma is a common cancer and cases have steadily increased since the mid *** some patients,early diagnosis and surgical removal of melanomas is lifesaving,while other patients typically turn to molecular targeted therapies and immunotherapies as treatment *** sampling of melanomas allows the scientific community to identify the most prevalent mutations that initiate melanoma such as the BRAF,NRAS,and TERT genes,some of which can be therapeutically *** initially effective,many tumors acquire resistance to the targeted therapies demonstrating the need to investigate compensatory *** represent an alternative to molecular targeted ***,inter-tumoral immune cell populations dictate initial therapeutic response and even tumors that responded to treatment develop resistance in the long *** the protocol for combination therapies develop,so will our scientific understanding of the many pathways at play in the progression of *** future direction of the field may be to find a molecule that connects all of the ***,noncoding RNAs have been shown to play important roles in melanoma development and *** noncoding RNAs may help us to understand how resistance e both primary and acquired e develops;ultimately allow us to harness the true potential of current *** review will cover the basic structure of the skin,the mutations and pathways responsible for transforming melanocytes into melanomas,the process by which melanomas metastasize,targeted therapeutics,and the potential that noncoding RNAs have as a prognostic and treatment tool.

关键词： BRAF inhibitors Checkpoint inhibitors Drug resistance Immunotherapy Melanoma Melanoma metastasis Skin cancer Targeted therapy Therapeutic resistance