检索结果-南通市图书馆

A novel mutation—L539fs/47 of hERG in a Chinese long QT syndrome family

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2010年第3期22卷 188-191页

作者： Jiang-Fang lian,Xiao-Yan Huang,Wei-Feng Xu,Xi Yang,Ying Wang,Di li,Jian-Qing Zhou li huili hospital,medical School of ningbo University,ningbo 315041,China li huili hospital Medical School of Ningbo University Ningbo 315041 China

Objective To identify the mutation of human ether-a-go-go-related gene(hERG)and analyze the clinical characteristics of a Chinese family with long ST syndrome(LQTS).Methods The electrocardiogram and DNA samples were obtained from a Chinese LQTS family of 26 *** was performed with polymorphic short tandem repeat(STR)markers at the known LQT1,LQT2,and LQT3 *** analysis was used to find aberrant *** mutation was confirmed by cloning and *** Three gene carriers were linked to chromosome 7q35-36,where the potassium channel gene hERG was encoded.A 19-base pair deletion was *** mutation was located at nucleotide position 1 619-1 637 between transmembrane domains S4 and ***,A1692G polymorphism was found both in the normal control and *** A novel 19 bp deletion mutation of hERG is identified in a Chinese *** gene carriers are demonstrated to be typical LQT2 ECG phenotype.

关键词： long QT syndrome human ether-a-go-go-related gene(hERG) potassium channel mutation

Combined detection of plasma GATA5 and SFRP2 methylation is a valid noninvasive biomarker for colorectal cancer and adenomas

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2015年第9期21卷 2629-2637页

作者： Xie Zhang Yu-Fei Song Hong-Na Lu Dan-Ping Wang Xue-Song Zhang Shi-liang Huang Bei-Lei Sun Zhi-Gang Huang Department of Gastroenterology Ningbo Medical Treatment Center Li Huili Hospital School of Medicine Ningbo University Department of Gastroenterology Zhejiang Provincial People’s Hospital

AIM:To investigate GATA5,SFRP2,and ITGA4 methylation in plasma DNA as noninvasive biomarkers for colorectal cancer(CRC) or ***:There were 57 CRC patients,30 adenomas patients,and 47 control patients enrolled in this ***-specific polymerase chain reaction was used to determine the promoter methylation status of GATA5,SFRP2,and ITGA4 genes in plasma DNA,and their association with clinical outcome in *** predictive ability of GATA5,SFRP2,and ITGA4 methylation,individually or in combination,to detect CRC or adenomas was further ***:Hypermethylated GATA5 was detected in plasma in 61.4%(35/57) of CRC cases,43.33%(13/30) of adenoma cases,and 21.28%(10/47) of control *** hypermethylation of SFRP2 was detected in 54.39%(31/57),40.00%(12/30),and 27.66%(13/47) in plasma samples from CRC,adenomas,and controls,***4 methylation was detected in 36.84%(21/57) of plasma samples of CRC patients and in 30.00%(9/30) of plasma samples from patients with colorectal adenomas,and the specificity of this individual biomarker was 80.85%(9/47).Moreover,GATA5 methylation in the plasma was significantly correlated with larger tumor size(P =0.019),differentiation status(P =0.038),TNM stage(P =0.008),and lymph node metastasis(P =0.008).SFRP2 and ITGA4 methylation in plasma significantly correlated with differentiation status(SFRP2,P =0.012; ITGA4,P =0.007),TNM stage(SFRP2,P =0.034; ITGA4,P =0.021),and lymph node metastasis(SFRP2,P =0.034; ITGA4,P =0.021).From the perspective of predictive power and cost-performance,using GATA5 and SFRP2 together as methylation markers seemed the most favorable predictor for CRC(OR =8.06;95%CI:2.54-25.5; P < 0.01) and adenomas(OR =3.35; 95%CI:1.29-8.71; P =0.012).CONCLUSION:A combination of GATA5 and SFRP2 methylation could be promising as a marker for the detection and diagnosis of CRC and adenomas.

关键词： Colorectal cancer GATA binding protein 5 Secreted

Design, synthesis and biological evaluation of chalcone analogues with novel dual antioxidant mechanisms as potential anti-ischemic stroke agents

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2019年第2期9卷 335-350页

作者： Jiabing Wang lili Huang Chanchan Cheng Ge li Jingwen Xie Mengya Shen Qian Chen Wulan li Wenfei He Peihong Qiu Jianzhang Wu Chemical Biology Research Center School of Pharmaceutical Sciences Wenzhou Medical University Municipal hospital Affiliated to medical School of Taizhou University ningbo medical centre li huili hospital College of Information Science and Computer Engineering Wenzhou Medical University

Scavenging reactive oxygen species(ROS) by antioxidants is the important therapy to cerebral ischemia-reperfusion injury(CIRI) in stroke. The antioxidant with novel dual-antioxidant mechanism of directly scavenging ROS and indirectly through antioxidant pathway activation may be a promising CIRI therapeutic strategy. In our study, a series of chalcone analogues were designed and synthesized, and multiple potential chalcone analogues with dual antioxidant mechanisms were screened. Among these compounds, the most active 33 not only conferred cytoprotection of H2 O2-induced oxidative damage in PC12 cells through scavenging free radicals directly and activating NRF2/ARE antioxidant pathway at the same time, but also played an important role against ischemia/reperfusion-related brain injury in animals. More importantly, in comparison with mono-antioxidant mechanism compounds, 33 exhibited higher cytoprotective and neuroprotective potential in vitro and in vivo. Overall, our findings showed compound 33 couldemerge as a promising anti-ischemic stroke drug candidate and provided novel dual-antioxidant mechanism strategies and concepts for oxidative stress-related diseases treatment.

关键词： Reactive oxygen species Cerebral ischemiareperfusion injury Stroke Dual-antioxidant mechanism Chalcones Antioxidants Oxidative stress NRF2/ARE

Author correction to ‘Design, synthesis and biological evaluation of chalcone analogues with novel dual antioxidant mechanisms as potential anti-ischemic stroke agents’ [Acta Pharmaceutica Sinica B 9 (2019) 335e350]

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2022年第8期12卷 3451-3452页

作者： Jiabing Wang lili Huang Chanchan Chen Ge li Jingwen Xie Mengya Shen Qian Chen Wulan li Wenfei He Peihong Qiu Jianzhang Wu Chemical Biology Research Center School of Pharmaceutical SciencesWenzhou Medical UniversityWenzhou 325035China Municipal hospital Affiliated to medical School of Taizhou University Taizhou 318000China ningbo medical centre li huili hospital Ningbo 315041China College of Information Science and Computer Engineering Wenzhou Medical UniversityWenzhou 325035China

The authors regret that there was a picture error in Fig.5D and Fig.5E,owing to the authors’mistakes of copying and pasting in the process of assembling figures and making a *** Fig.5D,the image of the edaravone 6 h group(ED-6h group)was repeated with the model group(NS group),and thus the image of the ED-6h group needs to be *** Fig.5E,the data of the edaravone 3h group(ED-3h group)was repeated with the 336h group(33-6h group),and thus the data of the ED-3h group needs to be corrected.

关键词： correction corrected figures

AAV mediated carboxyl terminus of Hsp70 interacting protein overexpression mitigates the cognitive and pathological phenotypes of APP/PS1 mice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第1期20卷 253-264页

作者： Zhengwei Hu Jing Yang Shuo Zhang Mengjie li Chunyan Zuo Chengyuan Mao Zhongxian Zhang Mibo Tang Changhe Shi Yuming Xu Department of Neurology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina Academy of medical Sciences of Zhengzhou University ZhengzhouHenan ProvinceChina NHC Key Laboratory of Prevention and treatment of Cerebrovascular Diseases The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina Henan Key Laboratory of Cerebrovascular Diseases The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina Institute of Neuroscience Zhengzhou UniversityZhengzhouHenan ProvinceChina Sino-British Research centre for Molecular Oncology National Centre for International Research in Cell and Gene TherapySchool of Basic Medical SciencesAcademy of Medical SciencesZhengzhou UniversityZhengzhouHenan ProvinceChina Department of Gerontology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina

The E3 ubiquitin ligase,carboxyl terminus of heat shock protein 70(Hsp70)interacting protein(CHIP),also functions as a co-chaperone and plays a crucial role in the protein quality control *** this study,we aimed to investigate the neuroprotective effect of overexpressed CHIP on Alzheimer’s *** used an adeno-associated virus vector that can cross the blood-brain barrier to mediate CHIP overexpression in APP/PS1 mouse *** overexpression significantly ameliorated the performance of APP/PS1 mice in the Morris water maze and nest building tests,reduced amyloid-βplaques,and decreased the expression of both amyloid-βand phosphorylated *** also alleviated the concentration of microglia and astrocytes around *** APP/PS1 mice of a younger age,CHIP overexpression promoted an increase in ADAM10 expression and inhibitedβ-site APP cleaving enzyme 1,insulin degrading enzyme,and neprilysin *** of HSP70 and HSP40,which have functional relevance to CHIP,were also *** nuclei transcriptome sequencing in the hippocampus of CHIP overexpressed mice showed that the lysosomal pathway and oligodendrocyte-related biological processes were up-regulated,which may also reflect a potential mechanism for the neuroprotective effect of *** research shows that CHIP effectively reduces the behavior and pathological manifestations of APP/PS1 ***,overexpression of CHIP could be a beneficial approach for the treatment of Alzheimer’s disease.

关键词： adeno-associated virus Alzheimer’s disease APP/PS1 mice carboxyl terminus of Hsp70 interacting protein gene therapy

Genomic and Phenotypic Diversity of Carbapenemase-Producing Enterobacteriaceae Isolates from Bacteremia in China: A Multicenter Epidemiological, Microbiological, and Genetic Study

在线全文

学校读者我要写书评

暂无评论

Engineering 2022年第5期8卷 90-100页

作者： Beiwen Zheng Hao Xu lihua Guo Xiao Yu Jinru Ji Chaoqun Ying Yunbo Chen Ping Shen Huiming Han Chen Huang Shuntian Zhang Tao Lv Yonghong Xiao State Key Laboratory for Diagnosis and Treatment of Infectious Diseases Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesThe First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhou 310003China medical College of Beihua University Jilin 132013China Department of Infectious Diseases Ningbo Medical Center Li Huili HospitalNingbo 315040China

Carbapenemase-producing Enterobacteriaceae(CPE) isolates are recognized as one of the most severe threats to public health. However, the population structure and genetic characteristics of CPE isolates among bloodstream infections(BSIs) are largely unknown. To address this knowledge gap, in this study,we included patients with clinically significant BSIs due to Enterobacterales isolates, recruited from 26 sentinel hospitals in China(2014–2015). CPE isolates were microbiologically and genomically characterized,including their susceptibility profiles, molecular typing, phylogenetic features, and genetic context analysis of carbapenemase-encoding genes. Of the 2569 BSI Enterobacterales isolates enrolled, 42(1.6%) were carbapenemase-positive. Moreover, among the 2242 investigated isolates, 1111(49.6%) extendedspectrum β-lactamase(ESBL)-producing isolates were identified in Escherichia coli(E. coli), Klebsiella pneumoniae(K. pneumoniae), Proteus mirabilis(P. mirabilis), and Klebsiella oxytoca. Whole genome sequencing analysis showed the clonal spread of K. pneumoniae carbapenemase(KPC)-2-producing K. pneumoniae sequence type(ST) 11 and New Delhi metallo-β-lactamase(NDM)-5-producing E. coli ST167 in our collection. Plasmid analysis revealed that carbapenemase-encoding genes were located on multiple plasmids. A high prevalence of biofilm-encoding type 3 fimbriae clusters and yesiniabactin-associated genes was observed in K. pneumoniae isolates. This work demonstrates the high prevalence of ESBLs and the wide dissemination of CPE among BSI isolates in China, which represent real clinical threats. Moreover, our findings first illustrate a more comprehensive genome scenario of CPE isolates among BSIs. The clonal spread of KPC-2-producing K. pneumoniae ST11 and NDM-5-producing E. coli ST167 needs to be closely monitored.

关键词： Carbapenemase Carbapenemase-producing Enterobacteriaceae Plasmid-mediated China Extended-spectrum b-lactamase

The Effect of integrated Traditional Chineseand Western Medicine on the Recovery of Patients with Early Acute Cerebral Hemorrhage

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Integrative Medicine 1996年第4期2卷 258-261页

作者：吴延林 li huili hospital of ningbo medical Center Ninbo Zhejiang Province

The author compared in this study the effects of the treatment with integrated traditional andwestern medicine with that of routine western medicine alone on the recovery of patients with acute hyperten-sive cerebral hemorrhage. The results of the treatments showed that integrated traditional Chinese and west-ern medicine had better effect on speeding up the absorption of intracranial hematoma, elimination of en-cephaledema and restoration of neural functions than routine western medicine. There is significant differencebetween the results of the two kinds of treatment (P< 0. 05￣0. 01 ) , which suggested that treating patientsearly with traditional Chinese medicine greatly helps the recovery of patients with cerebral hemorrhage.

关键词： cerebral hemorrhage intracranial hematoma tonify the Kidney and promote blood flow,restoration of neural function

Two Cases of Autoimmune Encephalitis with Multiple Anti-neuronal Antibodies

在线全文

学校读者我要写书评

暂无评论

Journal of Clinical and Nursing Research 2024年第8期8卷 15-20页

作者： Guomin Xie Wen Zhao Xiping Wu Department of Neurology Ningbo Medical Center Li Huili HospitalNingbo UniversityNingboZhejiang 315040China

Autoimmune encephalitis(AE)can arise from various etiologies and present with complex clinical manifestations,especially in cases involving multiple anti-neuronal *** report presents two cases of AE with multiple anti-neuronal antibodies admitted to ningbo medical Center li huili hospital on October 9,2020,and March 12,*** 1 is a 15-year-old boy with positive anti-N-methyl-D-aspartate receptor(NMDAR)and anti-metabotropic glutamate receptor 5(mGluR5)antibodies in his serum and cerebrospinal fluid(CSF).Case 2 is a 14-year-old boy with positive NMDAR and myelin oligodendrocyte glycoprotein(MOG)antibodies in his *** with AE who have multiple anti-neuronal antibodies present significant diagnostic and therapeutic challenges,warranting close clinical attention.

关键词： Autoimmune encephalitis Anti-neuronal antibody Treatment

Comparing the inter-observer reliability of the Tada formula among neurosurgeons while estimating the intracerebral haematoma volume

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Comparing the inter-observer reliability of the Tada formula...