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Loss of Masticatory Function Affects Morphology of the Tooth Root in Rats

Open Journal of Stomatology 2021年第1期11卷 37-54页

作者： Takamichi Yamada Kei Ogawa Takehiko Shimizu Department of Pediatric dentistry Nihon University Graduate School of Dentistry at Matsudo Chiba Japan Department of Pediatric dentistry Nihon University School of Dentistry at Matsudo Chiba Japan nihon university Research Institute of Oral Science Chiba Japan

Masticatory hypofunction and soft food affect the tooth rows, occlusion, and jawbone. This study aimed to clarify the influence of tooth loss and a soft diet on morphology of the tooth root during the growth period. We divided 3-week-old Wistar rats into the following three groups: Hard diet group (rats raised on solid standard diet), Powder diet group (rats raised on powdered standard feed diet), and Extraction group (rats raised on powdered standard diet with maxillary molars extraction). Length, width, cross-sectional area, and volume of the root of the mandibular M1 and M2 were measured using micro-CT analysis. Non-decalcified thin-slice specimens of sagittal sections of the M1 were obtained at the age of 20 weeks, and the roots were observed. The root length of all roots in the Extraction group was significantly longer than that in the other groups. The root width and cross-sectional area at the apical side 1/4 of all roots in the Extraction group were significantly smaller than those in the other groups. The root volume of the M1 mesial root in the Extraction group was significantly smaller than that in the other groups. This study clarified that when masticatory stimulus in the immature teeth is reduced by the extraction of opposing teeth and a powder diet, the root length increases due to the promotion of cellular cementum addition at the apex, and the root width and cross-sectional area decrease due to the suppression of cellular cementum addition at the apical side 1/4 of the roots.

关键词： Rats Tooth Root Morphology Powder Diet Extraction of Molars Micro-CT

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Pemphigus vulgaris macroscopically and cytologically resembling oral squamous cell carcinoma

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Open Journal of Stomatology 2012年第1期2卷 33-38页

作者： Kayo Kuyama Yan Sun Hiroyasu Endo Etsuko Kaneda Miyuki Morikawa Masanobu Wakami Takanori Ito Hirotsugu Yamamoto Department of Crown and Bridge Prosthodontics Nihon University School of Dentistry at Matsudo Matsudo City Japan Department of Diagnostic Pathology Hospital of Nihon University School of Dentistry at Matsudo Matsudo City Japan Department of Oral Diagnosis Nihon University School of Dentistry at Matsudo Matsudo City Japan Department of Oral Pathology Nihon University School of Dentistry at Matsudo Matsudo City Japan Department of Pathology Nihon University School of Dentistry Chiyoda-Ku Japan

We describe the clinical, macroscopic, cytological, histopathological, immunohistochemical, serodiagnostic and aspects of pemphigus vulgaris (PV) in the oral gingiva that clinically mimicked oral squamous cell carcinoma (OSCC) in a 57-year-old Japanese man. He developed slight haphalgesia of the buccal gingiva around teeth numbers 18 and 19 2 years ago. A dentist diagnosed intractable ulcer, but the patient ignored the condition for about 2 years until a sharp pain in the gingiva worsened. He consulted an otolaryngologist, who referred the patient to our hospital under a cytological diagnosis of OSCC. An oral examination revealed several extensive painful erosions/ ulcers from the buccal and lingual gingiva around teeth numbers 18 to 21 to the distal alveolar mucosa of no. 18 and the buccal and lingual gingiva around tooth number 31. A presumptive diagnosis of PV with dysplastic changes was determined from cytological smears. The cytological Nikolsky test was positive. The diagnosis of PV was confirmed from clinical and histopathological findings of a biopsy specimen obtained from the perilesional site. Although the definitive diagnosis of PV required only 2 weeks after this patient presented at our hospital, 2 years had elapsed since the onset of oral lesions.

关键词： Pemphigus Vulgaris Oral Squamous Cell Carcinoma Exfoliative Cytology

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Histopathological Examination and Literature Review of Orthokeratinized Odontogenic Cyst

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Open Journal of Stomatology 2019年第5期9卷 125-135页

作者： Hirotoshi Iwai Takehiro Suzuki Tomoko Suzuki Juri Muramori Hiroyuki Seto Mitsuko Nakayama Tadahiko Utsunomiya Masaaki Suemitsu Kayo Kuyama Satoshi Hirayama Department of Operative dentistry Nihon University School of Dentistry at Matsudo Chiba Japan Department of Pathology Nihon University School of Dentistry at Matsudo Chiba Japan

Background: With transitions in the disease concept of orthokeratinized odontogenic cyst (OOC), the pathogenesis and etiology have not been sufficiently elucidated. Objectives: OOC cases were reclassified and observed to understand the clinico-histopathological characteristics. In addition, literature review of OOC was performed to better organize the pathology. Materials and methods: Subjects with jawbone cysts lined by keratinized stratified squamous epithelium from 2005 to 2018 were reclassified, and clinico-histopathological findings were analyzed. Previous reports of OOC/orthokeratinized-type odontogenic keratocyst (OKC) from 1980 to 2019 were organized. Results: Five cases of OOC were diagnosed, representing 2.1% of odontogenic developmental cysts (total, 239 cases). Mean age was 37.6 years, with a female predominance. The mandibular molar area was the frequent site, and all cysts were solitary. Sixty percent involved an impacted tooth. Mean maximum diameter of the cyst was 2.2 cm. Histopathologically, 4 cases were unilocular, and partial palisading of the basal layer and scattered epithelial islands were observed in 2 cases each and formation of daughter cysts was noted in 3 cases. Previous papers described that most were unilocular and related to impacted teeth. Mean age ranged between 20 and 40 years, and the mandibular molar region was dominant. Recurrence rates were low. Conclusion: OOC shows a different biological attitude to OKC and is closer to dentigerous cyst. Meanwhile, OOC shows a similar histology of epidermoid cysts accompanying the granular layer. We thus surmised that OCC represents an independent concept as an odontogenic developmental cyst.

关键词： Orthokeratinized Odontogenic Cyst (OOC) Odontogenic Developmental Cyst Literature Review

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Pleomorphic Adenoma Consisting of Multiple Cysts with Squamous Epithelial Lining: Findings on MRI, FNAC, and Histopathological Examination

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Open Journal of Stomatology 2021年第6期11卷 221-230页

作者： Hiroshi Yamamoto Sakurako Yamaguchi Erika Iwai Yukiko Iizuka Shu Fushimi Kunio Hayashi Takumi Kondo Satoshi Tokunaga Masaaki Suemitsu Takashi Kaneda Kayo Kuyama Masamichi Komiya Department of Oral Surgery Nihon University School of Dentistry at Matsudo Chiba Japan Department of Radiology Nihon University School of Dentistry at Matsudo Chiba Japan Department of Pathology Nihon University School of Dentistry at Matsudo Chiba Japan

The incidence of pleomorphic adenoma (PA) of the minor salivary glands is reported to be 10%, and while the histological findings in PA can be diverse, keratin-filled cysts lined by squamous epithelium are rarely reported. The condition can, however, present with cyst formation in some cases. We review a rare case of pleomorphic adenoma in the buccal mucosa that involved the formation of multiple squamous epithelium-lined cysts in a 69-year-old woman. Magnetic resonance imaging (MRI), fine needle aspiration cytology, and histopathological examination were performed. Physical examination revealed a painless, mobile, elastic hard mass in the right buccal mucosa, measuring 2.5 × 1.0 cm. The MRI revealed a well-defined lesion with different signal intensities in the medial and distal regions of the right cheek. The medial side of the lesion showed a low signal intensity on T1-weighted imaging (T1WI) and T2-weighted imaging (T2WI), while the distal side showed a low signal intensity on T1WI, and a high signal intensity on T2WI and short tau inversion recovery (STIR) imaging. Fine needle aspiration of the lesion was performed under local anesthesia and a cytological diagnosis of an epidermoid or dermoid cyst was made. The tumor was completely resected under local anesthesia combined with intravenous sedation. The histopathological examination demonstrated the proliferation of atypical tumor cells with poor atypia and the formation of glandular, alveolar, large, and small cysts. The cysts were lined by keratinized squamous epithelial cells, their cavities were filled with keratinous material, and foreign body reaction was observed after rupture. Histopathological evaluation led to the diagnosis of pleomorphic adenoma. The patient had no evidence of recurrence, 2 years and 3 months after the surgery. In conclusion, the presence of multiple cysts lined by squamous epithelium can pose a significant diagnostic challenge in patients with PA. It is important to make the correct

关键词： Salivary Gland Tumor Pleomorphic Adenoma Cystic Formation

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Nontraumatic Myositis Ossificans in a Muscle of Mastication

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Open Journal of Stomatology 2016年第5期6卷 135-139页

作者： Tadahiko Utsunomiya Masaaki Suemitsu Miyuki Morikawa Masanobu Matsuno Hirotsugu Yamamoto Hitomi Sakata Kazuhiro Hasegawa Masahiro Higuchi Kayo Kuyama Department of Oral Pathology Nihon University School of Dentistry at Matsudo Matsudo Japan Department of Anatomy Nihon University School of Dentistry at Matsudo Matsudo Japan

Myositis ossificans (MO) is an extraskeletal tumor-like lesion with bone formation, and is relatively rare in the head and neck region. We report herein a clinicopathological analysis of MO associated with a muscle of mastication. The patient was a 70-year-old Japanese woman who presented with a tumorous mass in the right parotid area and no history of trauma. The surgically excised tumorous lesion consisted of proliferating fibroblastic cells with no atypia in the central portion, and a formation of trabecular osteoid and/or bony tissue in the peripheral portion as the so-called “zonal phenomenon”. The final histological diagnosis was MO, and the lesion was located in the posterior belly of the digastric muscle on computer tomography. A review of the literature showed that many cases of MO predominantly affected the medial pterygoid and masseter muscle and showed a history of previous trauma. The present case represents a rare case of nontraumatic MO in the uncommon location of the posterior belly of the digastric muscle.

关键词： Myositis Ossificans Muscle of Mastication Heterotopic Ossification Histopathology

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Study on Distribution of Four Pseudomonas Species in Living Environment Using Multiplex PCR

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Open Journal of Stomatology 2024年第2期14卷 77-86页

作者： Sachiyo Hayashi Koji Umezawa Osamu Tsuzukibashi Akira Fukatsu Mana Fuchigami Chiaki Komine Hiroshi Yamamoto Mio Hagiwara-Hamano Yukiko Iizuka Satoshi Uchibori Masanobu Wakami Hiroshi Murakami Taira Kobayashi Masahiko Fukumoto Takato Nomoto Department of Special Needs dentistry Nihon University School of Dentistry at Matsudo Chiba Japan Department of Laboratory Medicine for dentistry for the Compromised Patient Nihon University School of Dentistry at Matsudo Chiba Japan Department of Oral Surgery Nihon University School of Dentistry at Matsudo Chiba Japan Department of Fixed Prosthodontics Nihon University School of Dentistry at Matsudo Chiba Japan Department of Oral Implantology Nihon University School of Dentistry at Matsudo Chiba Japan

Purpose: The genus Pseudomonas is a ubiquitous microorganism frequently detected from immunocompromised patients. The inherent resistance to numerous antimicrobial agents contributes to the opportunistic character of this pathogen exhaustive monitoring of this pathogen is considered of critical importance to public health organizations. The reliable identification method able to distinguish genetic close Pseudomonas species is needed, because these organisms are difficult to differentiate by phenotypic or biochemical methods. The purpose of the present study was to design species-specific primers in order to identify and detect four Pseudomonas species which are frequently detected from the human oral cavities, and to investigate the distribution of these organisms in the living environment using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the rpoD gene of four Pseudomonas species. Swab samples were collected from fifty washstands, and the distribution of Pseudomonas species was investigated using a conventional PCR at genus level and a multiplex PCR at species level. Results: Multiplex PCR method developed in this study was able to distinguish four Pseudomonas species clearly. The genus Pseudomonas was detected from all samples (100%), whereas P. putida, P, aeruginosa, P. stutzeri and P. fluorescens were detected at 44%, 8%, 4% and 2% in fifty swab samples, respectively. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction. It was indicated that washstands were the uninhabitable environment for P. putida, P, aeruginosa, P. stutzeri and P. fluorescens.

关键词： Genus Pseudomonas Multiplex PCR Living Environment

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One Step Multiplex PCR for Identification of Candida haemulonii complex and Candia auris

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Advances in Microbiology 2023年第12期13卷 602-613页

作者： Mana Fuchigami Osamu Tsuzukibashi Akira Fukatsu Koji Umezawa Sachiyo Hayashi Yuji Takahashi Hiroshi Yamamoto Chiaki Komine Mio Hagiwara-Hamano Yukiko Iizuka Satoshi Uchibori Masanobu Wakami Hiroshi Murakami Taira Kobayashi Masahiko Fukumoto Department of Laboratory Medicine for dentistry for the Compromised Patient School of Dentistry at Matsudo Nihon University Chiba Japan Department of Special Needs dentistry School of Dentistry at Matsudo Nihon University Chiba Japan Department of Oral Implantology School of Dentistry at Matsudo Nihon University Chiba Japan Department of Oral Surgery School of Dentistry at Matsudo Nihon University Chiba Japan Department of Fixed Prosthodontics School of Dentistry at Matsudo Nihon University Chiba Japan

Purpose: Recently, Candida haemulonii complex (Candida haemulonii, Candida duobushaemulonii and Candida haemulonii var. vulnera) and two genetically close species (Candida pseudohaemulonii and Candida auris) have emerged as an opportunistic fungal pathogen associated with various infectious diseases of humans, and most of those isolates have displayed antifungal resistance. Because it is difficult to differentiate these microorganisms by a current technique, unfortunately, it is important to establish a method for identifying them accurately. The purpose of the present study was to design species-specific primers in order to identify and detect C. auris, C. pseudohaemulonii, and C. haemulonii complex, i.e. , C. haemulonii, C. duobushaemulonii and C. haemulonii var. vulnera using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the 26S rRNA, 18S rRNA, and RPB1 genes and ITS region of five Candida species. Results: The multiplex PCR method developed in this study was able to distinguish five Candida species clearly. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction.

关键词： Genus Candida Candida haemulonii complex Multiplex PCR Candida auris

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Comparative Study of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry and Culture Test for <i>Candida</i>Identification

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Open Journal of Stomatology 2019年第12期9卷 295-306页

作者： Yoriko Kubota Chieko Taguchi Masanori Saito Noriko Shinozaki-Kuwahara Tomoko Suzuki Masaaki Suemitsu Mitsuko Nakayama Tadahiko Utsunomiya Hiroyasu Endo Kayo Kuyama Department of Pathology Nihon University School of Dentistry at Matsudo Chiba Japan Department of Microbiology and Immunology Nihon University School of Dentistry at Matsudo Chiba Japan Department of Community Oral Health Nihon University School of Dentistry at Matsudo Chiba Japan Department of Oral Diagnosis Nihon University School of Dentistry at Matsudo Chiba Japan

Background: A new microorganism identification method using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) has been developed, however, reports on its use for delineating Candida spp. are scarce. Objectives: The purpose of this study was to compare the identification accuracy of mixed infection between culture test and MALDI-TOF MS. Materials and Methods: Eighty-nine denture wearers (average 74.0 ± 9 years) were selected. Specimens were immediately inoculated onto selective medium for CHROMagarTM Candida, and were also carried out using MALDI-TOF MS. The distribution frequencies of them were analyzed. Results: The numbers and rates of detection/non-detection by MALDI-TOF MS of genus Candida were 58/31 (65.2%/34.8%), respectively. Infection types were single infection in 34 (38.2%), mixed infection in 24 (27.0%), and non-infection in 31 (34.8%) cases. Concerning the single infection, C. albicans was the most predominant (58.8%), followed by C. parapsilosis (17.6%), C. glabrata (14.7%), C. tropicalis (5.9%), and C. krusei (2.9%). As for the mixed infection, the most frequent combination was C. albicans and C. glabrata (50.0%), followed by C. albicans and C. parapsilosis (29.2%), C. albicans and C. tropicalis (8.3%), C. glabrata and C. tropicalis (4.2%), C. albicans, C. glabrata, and C. parapsilosis (4.2%), and C. albicans, C. parapsilosis, and C. glabrata (4.2%). There were four MALDI-TOF MS positive results that were negative by the culture test. Conversely, there were six MALDI-TOF MS negative results that were positive by the culture test. The sensitivity and specificity of MALDI-TOF MS were 0.929 and 0.840, respectively. The concordance rate of genus Candida was 0.644, indicating substantial agreement. Conclusion: Candida infection is complicated by disease type and oral cavity environment changes due to aging. A rapid microorganism detection method, such as MALDI-TOF MS, will be helpful to quickly determine the causative pathogen in

关键词： Candida Culture Test MALDI-TOF MS Non-C. Albicans Candida (NCAC)

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Investigation of Bacteria Species Most Involved in Peri-Implantitis

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Open Journal of Stomatology 2023年第10期13卷 353-366页

作者： Hiroshi Murakami Osamu Tsuzukibashi Akira Fukatsu Yuji Takahashi Keisuke Idei Keisuke Usuda Mana Fuchigami Chiaki Komine Satoshi Uchibori Koji Umezawa Sachiyo Hayashi Takashi Asano Masanobu Wakami Taira Kobayashi Masahiko Fukumoto Department of Oral Implantology Nihon University School of Dentistry at Matsudo Chiba Japan Department of Laboratory Medicine for dentistry for the Compromised Patient Nihon University School of Dentistry at Matsudo Chiba Japan Department of Fixed Prosthodontics Nihon University School of Dentistry at Matsudo Chiba Japan Department of Special Needs dentistry Nihon University School of Dentistry at Matsudo Chiba Japan

Purpose: Currently, bacteriological examinations of implant treatments target periodontopathic bacteria such as red complex bacteria, including Porphyromonas gingivalis, and detect them qualitatively or quantitatively. However, it seems that those examinations do not reflect the peri-implant tissue conditions precisely, because periodontopathic bacteria are also frequently detected from healthy peri-implant sites. The purpose of the present study was to investigate bacteria species most involved in peri-implantitis using a PCR method. Methods: Polymerase chain reaction (PCR) primers in this study were designed based on partial sequences of 16S rDNA of bacteria species involved in peri-implantitis that were described in numerous previous studies. Peri-implant sulcus fluid (PISF) samples were collected from thirty periodontally healthy patients with implants (HI) and thirty patients with peri-implantitis (PI). Each detection frequency of bacteria species in PISFs of both groups was investigated using a PCR method, and was compared using Fisher’s exact test. Results: In PI group, detection frequencies of Corynebacterium durum, Fretibacterium fastidiosum and Slackia exigua were significantly higher than those of HI group (p P. gingivalis and Tannerella forsythia belonging to red complex were frequently detected in the PISF samples of HI group (p > 0.05). Conclusion: It was suggested that monitoring C. durum and F. fastidiosum levels in PISF samples was useful as a clinical indicator for the evaluation of peri-implant tissue conditions.

关键词： Peri-Implantitis PCR Method Bacteria Flora in Peri-Implant Sulcus Red Complex

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One-Step Multiplex PCR for Simultaneous Detection and Identification of Eight Medically Important <i>Candida</i>Species

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Open Journal of Stomatology 2021年第1期11卷 14-24页

作者： Akira Fukatsu Osamu Tsuzukibashi Hidenori Suzuk Katsuhiro Asaka Yoshinori Ono Mana Fuchigami Taira Kobayashi Satoshi Uchibori Yuji Takahashi Chiaki Komine Yoshimi Konishi Yuki Ogura Hiroko Omori Masanobu Wakami Hiroshi Murakami Masahiko Fukumoto Division of Laboratory Medicine for dentistry Department of Oral Health Science Nihon University School of Dentistry at Matsudo Chiba Japan Laboratory Medicine for dentistry Nihon University Graduate School of Dentistry at Matsudo Chiba Japan Department of Fixed Prosthodontics and Oral Implantology Nihon University School of Dentistry at Matsudo Chiba Japan Division of Oral Function and Rehabilitation Department of Oral Health Science Nihon University School of Dentistry at Matsudo Chiba Japan

Recently, the incidence of Candida infections has substantially increased. Conventional identification methods for Candida species are technically difficult to conduct and cannot accurately distinguish each species. The purpose of the present study was to design primers to identify and detect simultaneously eight medically important Candida species using one-step multiplex PCR. PCR primers were designed based on partial sequences of intergenic spacer (IGS) and internal transcribed spacer (ITS) genes of eight medically important Candida species. These primers were able to distinguish each Candida species and did not display cross-reactivity with representative Candida species other than the eight Candida species. Moreover, our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and worked without requiring DNA extraction.

关键词： Candida Candida albicans One-Step Multiplex PCR PCR Method

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