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Validity of long-term and short-term recall of occupational sitting time in Finnish and Chinese office workers

Journal of Sport and Health Science 2020年第4期9卷 345-351页

作者： Ying Gao Neil J.Cronin Nina Nevala Taija Finni neuromuscular research Center Faculty of Sport and Health SciencesUniversity of Jyv€askyl€aJyv€askyl€a FI-40014Finland Finnish Institute of Occupational Health Helsinki FI-00250Finland

Background:As sedentary behavior is a global health issue,there is a need for methods of self-reported sitting *** accuracy and reliability of these methods should also be tested in various populations and different cultural *** study examined the validity of longterm and short-term recall of occupational sitting time in Finnish and Chinese ***:Two cohort groups of office-based workers(58.6%female,age range 2267 years)participated:a Finnish group(FIN,n=34)and a Chinese group(CHI,n=36).Long-term(past 3-month sitting)and short-term(daily sitting assessed on 5 consecutive days)single-item measures were used to assess self-reported occupational sitting *** from each participant were compared to objectively measured occupational sitting time assessed via thigh-mounted accelerometers,with Spearman’s rho(r)used to assess validity and the Bland-Altman method used to evaluate *** of variation depicted day-to-day variability of time spent on sitting at ***:In the total study sample,the results showed that both long-term and short-term recall correlated with accelerometer-derived sitting time(r=0.532,95%confidence intervals(CI):0.3360.684,p<0.001;r=0.533,95%CI:0.4490.607,p<0.001,respectively).Compared to objectively measured sitting time,self-reported occupational sitting time was 2.4%(95%CI:0.5%to 5.3%,p=0.091)and 2.2%(95%CI:0.7%3.6%,p=0.005)greater for long-term and short-term recall,*** agreement level was within the range21.2%to 25.9%for long-term recall,and24.2%to 28.5%for short-term *** a 5-day work week,day-to-day variation of sitting time was 9.4%§11.4%according to short-term recall and 10.4%§8.4%according to accelerometry-derived occupational sitting ***:Overall,both long-term and short-term self-reported instruments provide acceptable measures of occupational sitting time in an office-based workplace,but their utility at the individual level is limited due

关键词： Accelerometry Daily recall Office workers Questionnaire Self-report Sitting time Validity

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Gene deletion and carrier detection in the family of Becker muscular dystrophy by short tandem repeat sequence polymorphism

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Chinese Medical Journal 1999年第3期112卷 50-53页

作者：蔡竖平沈定国汪江 Division of neuromuscular Disorders research Chinese General Hospital of PLA Beijing 100853 China

Objective To type haplotypes among the patients, carriers and normal offspring in a family of males with Becker muscular dystrophy in one generation by allelic fragment length polymorphism analysis. Methods Deletion analysis of the patients were performed using multiplex polymerase chain reaction (PCR) of amplification with 9 dystrophin exon primers. Intragenic short tandem repeat (STR) sequence (STR44, STR45, STR49 and STR50) were amplified by PCR to analyse allelic fragment length polymorphisms in the members of the family. Results The deletions of exons 17, 19 and 45, as well as deletions of allelic fragments at the loci of STR44 and STR45 were determined in the patients. Hemizygosity at those two loci were detected and carrier status ascertained in the mother of the patients. The normal haplotypes were typed in the sister of the patients. Conclusion The method of STR sequence polymorphism analysis can determine haplotypes at normal status or at risk status. It would be used in prenatal diagnosis and carrier detection in the families of Duchenne and Becker muscular dystrophy.

关键词： muscular dystrophy polymerase chain reaction repetitive sequences

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Chronic training status affects muscle excitation of the vastus lateralis during repeated contractions

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Sports Medicine and Health Science 2023年第1期5卷 42-49页

作者： Sunggun Jeon Stephanie A.Sontag Trent J.Herda Michael A.Trevino Applied neuromuscular Physiology laboratory Department of KinesiologyApplied Healthand RecreationOklahoma State UniversityStillwaterOKUSA Neuromechanics laboratory Department of HealthSportand Exercise SciencesThe University of KansasLawrenceKSUSA

This study examined electromyographic amplitude(EMGRMS)-force relationships during repeated submaximal knee extensor muscle actions among chronic aerobically-(AT),resistance-trained(RT),and sedentary(SED)*** adults(5/group)attempted 20 isometric trapezoidal muscle actions at 50%of maximal *** electromyography(EMG)was recorded from vastus lateralis(VL)during the muscle *** the first and last successfully completed contractions,linear regression models were fit to the log-transformed EMGRMS-force relationships during the linearly increasing and decreasing segments,and the b terms(slope)and a terms(antilog of y-intercept)were *** was averaged during steady *** the AT completed all 20 muscle *** the first contraction,the b terms for RT(1.3010.197)were greater than AT(0.9100.123;p?0.008)and SED(0.9120.162;p?0.008)during the linearly increasing segment,and in comparison to the linearly decreasing segment(1.0180.139;p?0.014),*** the last contraction,the b terms for RT were greater than AT during the linearly increasing(RT?1.3730.353;AT?0.8830.129;p?0.018)and decreasing(RT?1.5260.328;AT?0.9700.223;p?0.010)*** addition,the b terms for SED increased from the linearly increasing(0.9680.144)to decreasing segment(1.2680.126;p?0.015).There were no training,segment,or contraction differences for the a *** during steady force increased from the first-([64.0851.68]μV)to last-contraction([86.7349.55]μV;p?0.001)collapsed across training *** b terms differentiated the rate of change for EMGRMS with increments in force among training groups,indicating greater muscle excitation to the motoneuron pool was necessary for the RT than AT during the linearly increasing and decreasing segments of a repetitive task.

关键词： Electromyography Fatigue Isometric trapezoidal muscle action Motor unit control properties Natural log-transformed model Vastus lateralis

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Advances in the differentiation of pluripotent stem cells into vascular cells

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World Journal of Stem Cells 2024年第2期16卷 137-150页

作者： Yi-Chang Jiao Ying-Xin Wang Wen-Zhu Liu Jing-Wen Xu Yu-Ying Zhao Chuan-Zhu Yan Fu-Chen Liu Department of Neurology Qilu Hospital of Shandong UniversityJinan 250012Shandong ProvinceChina research Institute of neuromuscular and Neurodegenerative Diseases Qilu Hospital of Shandong UniversityJinan 250012Shandong ProvinceChina Mitochondrial Medicine laboratory Qilu Hospital(Qingdao)of Shandong UniversityQingdao 266103Shandong ProvinceChina Brain Science research Institute Shandong UniversityJinan 250012Shandong ProvinceChina

Blood vessels constitute a closed pipe system distributed throughout the body,transporting blood from the heart to other organs and delivering metabolic waste products back to the lungs and *** in blood vessels are related to many disorders like stroke,myocardial infarction,aneurysm,and diabetes,which are important causes of death *** research for new appro-aches to disease modeling and effective treatment is needed due to the huge socio-economic burden on healthcare *** mice or rats have been widely used,applying data from animal studies to human-specific vascular physiology and pathology is *** rise of induced pluripotent stem cells(iPSCs)provides a reliable in vitro resource for disease modeling,regenerative medicine,and drug discovery because they carry all human genetic information and have the ability to directionally differentiate into any type of human *** review summarizes the latest progress from the establishment of iPSCs,the strategies for differentiating iPSCs into vascular cells,and the in vivo trans-plantation of these vascular *** also introduces the application of these technologies in disease modeling,drug screening,and regenerative ***,the application of high-tech tools,such as omics analysis and high-throughput sequencing,in this field is reviewed.

关键词： Induced pluripotent stem cell Blood vessels Vascular organoids Endothelial cells Smooth muscle cells Pericytes Tissue engineering vascular graft

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Alterations in neuromuscular activation patterns associated with walking in short-leg walking boots

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Journal of Sport and Health Science 2012年第1期1卷 43-48页

作者： Douglas Powell Kurt Clowers Maria Keefer Songning Zhang neuromuscular Biomechanics laboratory Fairmont State UniversityFairmontWVUSA Anthropometry and Biomechanics Facility NASA Johnson Space CenterHoustonTXUSA University of Texas Health Science Center HoustonTXUSA Biomechanics/Sports Medicine laboratory The University of TennesseeKnoxvilleTNUSA

Short-leg walking boots are a common intervention for acute and chronic lower extremity *** studies have examined the neuromuscular adaptations associated with short-leg walking boots and no previous study has investigated timing characteristics of muscle activation during *** purpose of the current study was to examine the timing and amplitudes of muscle activation of the extrinsic ankle musculature during walking in two types of short-leg walking ***:Eleven healthy young adults performed five level walking trials at a self-selected pace in each of three conditions:normal walking,Gait Walker and Equalizer short-leg walking *** reaction forces were collected from a force platform while surface electromyography (EMG)was collected from the tibialis anterior,peroneus longus and medial *** signals were rectified and smoothed using the root mean squared with a 20-ms smoothing window and were normalized to the largest mean of the normal walking trials.A repeated measures analysis of variance was used to assess the effect of short-leg walking boots on the onset,duration and amplitude of muscle ***:Short-leg walking boots were generally associated with earlier onsets of muscle activation and longer durations of muscle ***,there was no reduction in EMG ***:The findings of this study show that the induced alterations in muscle activation patterns may limit the short-leg walking ***(C)2012,Shanghai University of *** and hosting by Elsevier *** rights reserved.

关键词： Electromyography Gait Short-leg walker Walking Walking boot

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Targeting muscle to treat Charcot-Marie-Tooth disease

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Neural Regeneration research 2024年第8期19卷 1653-1654页

作者： David Villarroel-Campos James N.Sleigh Department of neuromuscular Diseases and UCL Queen Square Motor Neuron Disease Centre UCL Queen Square Institute of NeurologyUniversity College LondonLondonUK UK Dementia research Institute University College LondonLondonUK

Charcot-Marie-Tooth disease(CMT)is a hereditary peripheral neuropathy causing muscle weakness/wasting and sensory dysfunction predominantly in limb *** patients display gait abnormalities,foot deformities,loss of sens... 详细信息

关键词： Marie sensation neuropathy

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Effects of areca nut consumption on cell differentiation of osteoblasts, myoblasts, and fibroblasts

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BIOCELL 2023年第2期47卷 283-287页

作者： YUNG-FU CHANG Department of Biomedical Science and Environmental Biology Kaohsiung Medical UniversityKaohsiung807Taiwan Department of Medical research Kaohsiung Medical University HospitalKaohsiung807Taiwan Translational research Center of neuromuscular Diseases Kaohsiung Medical University HospitalKaohsiung807Taiwan

Areca nut is used worldwide as a hallucinogenic addicting drug along the tropical ***,a toxic compound,is the most important alkaloid in areca *** adverse effects of oral uptake and chewing of areca nut are well *** example,the possibility of cancer caused by chewing areca nuts is widely *** areca nut has other adverse effects on other organs,including abnormal cell differentiation,oral cancer,and several other *** use of areca nut is also associated with low *** musculature is the largest organ in the body and is attached to the *** embryo development,the differentiation of bone and muscle cells is *** this article,we reviewed the effects of areca nut and arecoline on embryonic cell differentiation,particularly osteoblasts,myoblasts,and fibroblasts.

关键词： Areca nut Cell differentiation Osteoblast Myoblast Fibroblast

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Reply to “Phenotypic and Genotypic Peculiarities in Chinese Patients with Leigh Syndrome”

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Chinese Medical Journal 2019年第5期132卷 628-629页

作者： Xiao-Lin Yu Yu-Ying Zhao Department of Geriatrics Medicine Qilu Hospital Shandong University Jinan Shandong 250012 China Department of Neurology Research Institute of Neuromuscular and Neurodegenerative Diseases Qilu Hospital Shandong UniversityJinan Shandong 250012 China

To the Editor: We really appreciate the readers5 interest and comments about our article, and to have the opportunity to make an explanation[1].

关键词： Phenotypic Genotypic Peculiarities Chinese Patients with Leigh Syndrome

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Upregulation of Interleukin 21 and Interleukin 21 Receptor in Patients with Dermatomyositis and Polymyositis

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Chinese Medical Journal 2017年第17期130卷 2101-2106页

作者： Tao Liu Ying Hou Ting-Jun Dai Chuan-Zhu Yan research Institute of neuromuscular and Neurodegenerative Diseases Department of Neurology Qilu Hospital Shandong University JinanShandong 250012 China Department of Neurology The Affiliated Hospital of Qingdao University Qingdao Shandong 266003 China research Institute of neuromuscular and Neurodegenerative Diseases Department of Neurology Qilu Hospital Shandong University JinanShandong 250012 China research Institute of neuromuscular and Neurodegenerative Diseases Department of Neurology Qilu Hospital Shandong University JinanShandong 250012 China Brain Science research Institute Shandong University Jinan Shandong 250012 China

Background：The immunopathologic mechanism underlying dermatomyositis （DM） and polymyositis （PM） remains poorly *** cytokines play a pathogenic role in DM and *** 21 （IL-21） has a pleiotropic effect on inflammation *** study aimed to detect the serum IL-21 level and investigate the expression of IL-21 and IL-21 receptor （IL-21 R） in muscle tissues of patients with DM and ***：Biopsied muscle samples were obtained from 11 patients with DM,12 with PM,and six controls;mRNA levels of IL-21 and IL-21 R were analyzed by real-time quantitative reverse transcription-polymerase chain reaction;and immunohistochemical staining was used to evaluate the protein expression of IL-21 and *** samples were obtained from 36 patients with DM,19 with PM,and 20 healthy *** serum IL-21 level was detected by enzyme-linked immunosorbent ***：The expression of IL-21 was upregulated in patients with DM and *** IL-21 mRNA level was significantly increased in muscle tissues of patients with DM and PM （DM ***,P =0.001;PM ***,P =0.001）,whereas IL-21R mRNA level in patients with DM/PM was not statistically different from that of healthy *** staining showed both I L-21 and IL-21R were significantly expressed in the inflammatory cells in muscle tissues of patients with DM and *** serum IL-21 level was also significantly higher in patients with DM/PM than in controls （DM ***,49.12 [45.28,60.07] pg/ml vs.42.54 [38.69,48.85] pg/ml,P =0.00l;PM ***,50.77 [44.19,60.62] pg/ml vs.42.54 [38.69,48.85] pg/ml,P =0.005）.Conclusions：IL-21 expression is upregulated in patients with DM and PM in both muscle tissue and *** addition,IL-21R protein is highly expressed in affected muscle tissues of patients with DM and ***-21 may play a pathogenic role through IL-21R in patients with DM and PM.

关键词： Dermatomyositis Interleukin 21: lnterleukin 21 Receptor Polymyositis

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Effects of blood flow restriction without additional exercise on strength reductions and muscular atrophy following immobilization:A systematic review

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Journal of Sport and Health Science 2020年第2期9卷 152-159页

作者： Mikhail Santos Cerqueira Jose Diego Sales Do Nascimento Daniel Germano Maciel Jean Artur Mendonca Barboza Wouber Herickson De Brito Vieira Department of Physical Therapy Laboratory of Muscle PerformanceFederal University of Rio Grande do NorteNatalRio Grande do Norte 59072-970Brazil Department of Physical Therapy Neuromuscular Performance Analysis LaboratoryFederal University of Rio Grande do NorteNatalRio Grande do Norte 59072-970Brazil

Purpose:To investigate whether blood flow restriction(BFR) without concomitant exercise mitigated strength reduction and atrophy of thigh muscles in subjects under immobilization for lower ***:The following databases were searched:PubMed,CINAHL,PEDro,Web of Science,Central,and ***:The search identified 3 eligible studies,and the total sample in the identified studies consisted of 38 *** and isometric torque of the knee flexors and extensors was examined in 2 ***-sectional area of thigh muscles was evaluated in 1 study,and thigh girth was measured in 2 *** BFR protocol was 5 sets of 5 min of occlusion and 3 min of free flow,twice daily for approximately 2 *** a whole,the included studies indicate that BFR without exercise is able to minimize strength reduction and muscular atrophy after *** is crucial to emphasize,however,that the included studies showed a high risk of bias,especially regarding allocation concealment,blinding of outcome assessment,intention-to-treat analyses,and group similarity at ***:Although potentially useful,the high risk of bias presented by original stodies limits the indication of BFR without concomitant exercise as an effective countermeasure against strength reduction and atrophy mediated by immobilization.

关键词： Disuse Hypoxia Ischemia Muscle wasting Rehabilitation

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