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Analysis of digenic epistatic effects and QE interaction effects QTL controlling grain weight in rice

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Journal of Zhejiang University Science 2004年第4期5卷 371-377页

作者：高用明朱军宋佑胜何慈信石春海邢永忠 AgronomyDepartment ZhejiangUniversityHangzhou310029China nationalkeylaboratoryofcropgeneticimprovement HuazhongAgriculturalUniversityWuhan430070China

Immortalized F2 population of rice (Oryza sativa L.) was developed by randomly mating F1 among recombinant inbred (RI) lines derived from (Zhenshan 97B×Minghui 63),which allowed replications within and across *** (quantitative trait loci) mapping analysis on kilo-grain weight of immortalized F2 population was performed by using newly developed software for QTL mapping,QTL Mapper 2.0. Eleven distinctly digenic epistatic loci included a total of 15 QTL were located on eight *** main effects of additive,dominance,and additive×additive,additive×dominance,and dominance×dominance interactions were *** effects between QTL main effects and environments (QE) were *** than 40% of single effects,most of which were additive effects,for identified QTL were significant at 5% *** directional difference for QTL main effects suggested that these QTL were distributed in parents in the repulsion *** should make it feasible to improve kilo-grain weight of both parents by selecting appropriate new recombinants. Only few of the QE interaction effects were *** prospect for QTL mapping achievements in genetic breeding was discussed.

关键词： Immortalized F_2 population Rice Kilo-grain weight QTL Epistasis QTL×environment

Transfer of bacterial blight resistance from Oryza meyeriana to O. sativa L. by asymmetric somatic hybridization

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Chinese Science Bulletin 2004年第14期49卷 1481-1484页

作者： ZHUYongsheng CHENBaotang YUShunwu ZHANGDuanpin ZHANGXueqin YANQiusheng nationalkeylaboratoryofcropgeneticimprovement HuazhongAgriculturalUniversityWuhan430070China ChinaNationalRiceResearchInstitute Hangzhou310006China

Asymmetric somatic hybrid plants were pro-duced between cultivated rice (Oryza sativa L.) and wild spe-cies [O. meyeriana (Zoll. etMor. exSteud.)] with high resis-tance to rice bacterial blight. X-ray-irradiated protoplasts of the wild species were used as donor and chemically fused with iodoacetamide-inactivated protoplasts of rice cv. 02428 to produce hybrids. Seventy-two plants were regenerated from 623 calli based on metabolic complementation. The morphological characters of the plants closely resembled that of the rice. Simple sequence repeats were employed to iden-tify their hybridity. Cytological analysis of root-tips revealed that their chromosome number varied in the range of 27— 38. The somatic hybrids were inoculated with strains of Xanthamonas oryzae pv. oryzae at adult growth stage and demonstrated the resistance to bacterial blight introgression from the O. meyeriana.

关键词：体细胞杂交水稻细菌疫病 Oryza sativa L. Oryza 水稻代谢互补

Analysis of SSR in Citrus Sequences from EMBL Database

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Agricultural Sciences in China 2005年第7期4卷 501-506页

作者： MENGHai-jun CAOQing-qin HUZhi-yong LIUGao-ping CHENGYun-jiang DENGXiu-xin nationalkeylaboratoryofcropgeneticimprovement HuazhongAgriculturalUniversityWuhan430070P.R.China nationalkeylaboratoryofcropgeneticimprovement HuazhongAgriculturalUniversityWuhan430070P.R.China／／DepartmentofBiotechnologyBeijingAgriculturalCollegeBeijing102206P.R.China

Abundance of simple sequence repeat (SSR) in Citrus sequences from EMBL database was investigated by usingcomputer program MISA (MIcroSAtellite), which aimed to provide useful information for the development of SSR *** 32 896 sequences of Citrus, 4 987 SSRs were found in 4 167 sequences and the average distance between SSRs wasapproximately 3.5 kb. Mononucleotide repeats (50.6%) were the most abundant repeats. And di-, tri-, tetra-, penta- andhexa-nucleotide repeats were 22.8, 25.2, 1, 0.08, and 0.36%, respectively. The most abundant motif was A/T followed indescending order by AG/CT, AC/GT, AT/TA. AAT/ATT, AAG/CTT, AGC/CGT, ACG/CTG and C/G. They comprised about90% of all microsatellites. Ten primer pairs were designed, and three of them produced clear visible bands among Citrusand its related genera.

关键词： Citrus Simple sequence repeats Abundance Markers

Genetic Diversity of Chinese and Swedish Rapeseed (Brassica napus L.) Analyzed by Inter-Simple Sequence Repeats (ISSRs)

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Agricultural Sciences in China 2003年第2期2卷 137-143页

作者： MAChao－zhi StineTuevesson 等 nationalkeylaboratoryofcropgeneticimprovement HuazhongAgriculturalUniversityWuhan430070P.R.China SuavofWeibullAB S－26881SuavlovSweden

We have compared genetic diversity of 24 Chinese weak-winter, Swedish winter and spring B. napus accessions by inter-simple sequence repeats (ISSRs). By cluster analysis (UPGMA) based on 125 polymorphism bands amplified with 20 primers, the 24 accessions were divided into three groups. Six Swedish winter lines and eight Chinese weak-winter lines were in the group I and the groupⅡwere two Chinese weak-winter lines XiangyoulS and Bao81. The third group contained eight Swedish spring lines. Principal co-ordinates analysis (PCO) showed similar groupings to cluster analysis. Results from cluster analysis and PCO analysis showed very clearly that Chinese weak-winter, Swedish spring and winter accessions were distinguished from each other and Chinese weak-winter accessions in this study were genetically closer to Swedish winter accessions than to Swedish spring accessions. The Chinese weak-winter accessions had larger diversity than Swedish spring or winter accessions did. This study indicated that ISSR is a suitable and effective tool to evaluate genetic diversity among rapeseed germplasm.

关键词： Brassica napus L. Genetic diversity Inter-simple sequence repeats (ISSRs)

Relationship Between Hybrid Performance and Genetic Diversity Based on SSRs and ISSRs in Brassica napus L.

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Agricultural Sciences in China 2003年第10期2卷 1083-1090页

作者： FUTing-dong YANGGuang-sheng SHENJin-xiong OilCropsResearchInstitute ChineseAcademyofAgriculturalSciencesWuhan430062P.R.China nationalkeylaboratoryofcropgeneticimprovement HuazhongAgriculturalUniversityWuhan430070P.R.China

To investigate the relationship between genetic distance (GD) and hybrid performance, two types of molecular markers, microsatellites (simple sequence repeats, SSRs) and intro-simple sequence repeats (ISSRs), were employed to detect the genetic diversity of 3 double low self-incompatible lines and 22 male parental varieties of Brassica napus from different geographical origins. Hybrids were produced in a NCⅡ mating design by hand-pollination. The result indicated that 25 parental varieties (lines) could be divided into six groups by Un-weighted Pair Group Mathematics Average (UPGMA) clustering based on GDs. SI-1300 and SI-1320 could be singly clustered into one group, respectively. Varieties from China could be separated into another group, SI-1310 and varieties from foreign countries could be separated into other three groups. The grouping was generally consistent with parental pedigrees and geographical origins. Significant differences in yield, quality and phenological period traits were observed among these parent groups. Although hybrid yield/plant showed significantly positive correlation with genetic distance based on SSR and ISSR markers, but the determination coefficient was low. It appeared to be unsuitable for using the genetic distance based on SSR and ISSR markers to predict heterosis and hybrid performance in Brassica napus.

关键词： Brassica napus L. Genetic diversity Hybrid performance SSR ISSR

Phylogenetic Analysis on SLG and SRK Genes in Brassica and Raphanus

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Agricultural Sciences in China 2005年第1期4卷 7-14页

作者： HEYu-tang MAChao-zhi FUTing-dong TUJin-xing nationalkeylaboratoryofcropgeneticimprovement NationalRapeseedImprovementSub-centerHuazhongAgriculturalUniversityWuhan430070P.R.China KeyLaboratoryofFoodQualityandSafetyandFunctionalFoodResearchofLiaoningProvince BiologyandFoodScienceCollegeBohaiUniversityJinzhou121000P.R.China

S-locus genes were cloned from three Brassica napus and three B. campestris lines by using PCR walking and homologuesequence methods. A phylogenetic gene tree was constructed based on the six cloned genes and fifty-one previouslyreported SLG/SRK genes of Brassica and Raphanus. The SLGs from R. sativus were dispersed in the phylogenetic treeintermingling with SLG/SRKs from B. oleracea, B. napus and B. campestris. The SLG/SRK genes of classⅡclusteredindependently in one group. The SLG/SRK genes of classⅠshowed to be more divergent than classⅡgenes. Theseresults suggested that the divergence of classⅠand classⅡ should have occurred before the differentiation of thegenera Brassica and Raphanus. In addition, SLG and SRK of the same S haplotypes belonged to the same class. Itsuggested that class-Ⅰ and class-Ⅱ group divergence occurred first, and then SLG and SRK diverged. The three SC SRKgenes from B. napus and B. campestris were grouped into one cluster, displaying difference from the SC SLG of ***. These three SC SRK genes were close to SI SRK of SI1300, SI271 and guanyou in phylogenetic *** results indicated that SC and SI genes diverged more recently. It is not clear yet whether the differentiation of SCand SI genes was earlier than the differentiation of Brassica and Raphanus. Studies based on more genes are necessaryfor a comprehensive elucidation of the phylogenetic relationships in Brassicaceae.

关键词： PCR walking Evolution Phylogenetic relationships S-locus gene

Genetic Transformation of Brassica campestris *** via Agrobacterium-with Bt Gene

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Hunan Agricultural Science ＆ Technology Newsletter 2002年第4期3卷 10-14页

作者： WangFeng LiHanxia YeZhibiao LuYong'en nationalkeylaboratoryofcropgeneticimprovement Wuhan43007China CollegeofHorticultureandForestry HuazhongAgriculturalUniversityWuhan43007China AuthorforcorrespondenceAcceptedSeptember30 2002

By means of Agrobacterium-mediated transformation, 43 kanamycin-resistant buds of Chinese cabbage were got. PCR, PCR-Southern blot and dot blot analysis were used to identify and characterize the putative transgenic plants. 26 plants had the predicted bands of the fragment of npt Ⅱ gene. Insect bioassays of 4 transformants showed that toxic protein had been translated and the translation levels were different among these transformants.

关键词： Chinese cabbage Bt gene genetic transformation cry IA(b)