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电热损伤法建立新西兰大白兔宫腔粘连模型（英文）

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2018年第5期19卷 383-389页

作者： Xin-xin XU Lian-bao CAO Zhe WANG Zhen XU Bing-qian ZHANG She-ling WU Sha-sha QI Lei YAN Zi-jiang CHEN center for reproductive Medicine Shandong Provincial Hospital Affiliated to Shandong University/National Research Center for Assisted Reproductive Technology and Reproductive Genetics/MOE Key Laboratory of Reproductive Endocrinology (Shandong University)/Shandong Provincial Key Laboratory of Reproductive Medicine center for reproductive Medicine the Fifth People’s Hospital of Jinan

目的:目前宫腔粘连(Intrauterine adhesion,IUA)发病机制及治疗方法仍未明确和统一,建立一个稳定有效的宫腔粘连动物模型是开展相关研究的前提和基础。本文旨在利用电热损伤法构建兔IUA模型,观察和评估该方法的建模效果。创新点:首次提... 详细信息

目的:目前宫腔粘连(Intrauterine adhesion,IUA)发病机制及治疗方法仍未明确和统一,建立一个稳定有效的宫腔粘连动物模型是开展相关研究的前提和基础。本文旨在利用电热损伤法构建兔IUA模型,观察和评估该方法的建模效果。创新点:首次提出利用电热损伤法建立兔宫腔粘连模型,并得出在损伤后7~14天内建立的兔IUA模型是有效的结论。方法:将21只成年雌性新西兰大白兔一侧子宫内膜用医用多功能高频电刀电灼损伤模拟宫腔粘连形成(A组,n=21),另一侧子宫不做处理作为自身对照(B组,n=21)。分别在损伤后7、14和28天收集兔双侧子宫组织,行苏木精-伊红染色法(HE)和Masson染色观察两侧子宫内膜病理改变,并对两侧子宫内膜的腺体个数和内膜纤维化面积比进行统计学分析和比较。另外,将损伤后7天的雌兔与成年雄兔合笼,14天后观察比较两侧子宫胚胎个数。结论:病理组织学观察显示,损伤后7和14天,A组子宫内膜腺体数量较B组减少,差异均有统计学意义(P<0.05);而损伤后30天,A组子宫内膜腺体数量与B组相比差异无统计学意义。损伤后7天,A组内膜纤维化面积较B组增大,差异有统计学意义(P<0.01);A组子宫胚胎个数较B组减少,差异有统计学意义(P<0.01)。综上所述,采用电热损伤法建立的兔宫腔粘连模型在损伤后7~14天是稳定有效的。

关键词：宫腔粘连电热损伤兔模型

Protect chromosomes from end-to-end fusion during meiotic bouquet

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Science China(Life Sciences) 2018年第6期61卷 736-738页

作者： Shunxin Wang Binyuan Zhai Xiao Yang Liangran Zhang center for reproductive Medicine Shandong University Shandong Provincial Hospital Affiliated to Shandong University The Key Laboratory of Reproductive Endocrinology of Ministry of Education National Research Center for Assisted Reproductive Technology and Reproductive Genetics Shandong Provincial Key Laboratory of Reproductive Medicine State Key Laboratory of Microbial technology Shandong University

Meiosis generates gametes with half the parental chromosome complements,and thus is crucial for successful sexual reproduction.During meiotic prophase I,a series of specialized events occur to ensure correct paternal and maternal homologous chromosomes(homologs)segregation during anaphase I.They include recognition,alignment,pairing and synapsis of the homologs and meiotic recombination

关键词：染色体端对端伤害熔化保护成熟分裂专业化事物

Low anti-mullerian hormone decreased clinical pregnancy and increased risk of poor ovarian response in women over 35 years of age

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Chinese Medical Journal 2023年第4期136卷 499-501页

作者： Jiaqi Chang Lihong Xu Yiming Qin Ran Liu Chenxi Li Shanshan Gao Yujie Dang center for reproductive Medicine Key Laboratory of Reproductive Endocrinology of Ministry of EducationShandong Key Laboratory of Reproductive MedicineShandong Provincial Clinical Research Center for Reproductive HealthNational Research Center for Assisted Reproductive Technology and Reproductive GeneticsCheeloo College of MedicineShandong UniversityJinanShandong 250012China Department of Gynaecology and Obstetrics Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinanShandong 250021China Shandong First Medical University JinanShandong 250021China

To the Editor:Infertility affects 15%to 20%of couples worldwide,and assisted reproductive technology(ART)is the most effective treatment strategy.In addition to female age,many other factors,such as antral follicle count(AFC),basal follicle-stimulating hormone(FSH)level,and anti-Mullerian hormone(AMH)level,collectively known as biomarkers for ovarian reserve tests(ORTs),are widely used to predict ovarian responses and assisted-pregnancy outcomes.[1]Among them,AMH and FSH levels were discordant in 20%to 43%of women undergoing ART treatment,and AMH was proven to be superior in predicting live birth rate(LBR).[2]

关键词： pregnancy collective treatment

Comparison of the Developmental Potential and Clinical Results of In Vivo Matured Oocytes Cryopreserved with Different Vitrification Media

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Chinese Medical Journal 2015年第22期128卷 3029-3034页

作者： Mei Li Miao-Miao Wang Hui Liu Ke-Liang Wu Shui-Ying Ma Cheng Li Hai-Bin Zhao Zi-Jiang Chen center for reproductive Medicine Provincial Hospital Affiliated to Shandong University National Research Center for Assisted Reproductive Technology andReproductive Genetics Key Laboratory for Reproductive Endocrinology of Ministry of EducatLon Shandong Provincial Key Laboratory of Reproductive Medicine Jinan Shandong 250021 China

Background： Oocyte vitrification is widely used throughout the world, but its clinical efficacy is inconsistent and depends on the vitrification media. This study compared the developmental potential and clinical results of in vivo matured oocytes cryopreserved with different vitrification media. Methods： This retrospective study involved vitrified-warmed oocytes at one in vitro fertilization laboratory. Vitrification media kits comprised the MC kit （ethylene glycol （EG] plus 1,2-propanediol [PROH]）, the KT kit （EG plus dimethyl sulphoxide [DMSO]）, and the Modified kit （EG plus DMSO and PROH kit）. Rates of oocyte survival and subsequent developmental potential were recorded and analyzed. The t-test and the Chi-square test were used to evaluate each method＇s efficacy. Results： Oocyte survival rate was significantly higher for the Modified kit （92.0%） than for the MC kit （88.2%）（P 〈 0.05） and the KT kit （77.3%）（P 〈 0.001）. The rate of high-quality embryo development in the Modified kit group （35.8%） was significantly higher than in the MC kit group （29.0%） and the KT kit group （28.3%）（P 〈 0.001 ）. No significant differences were observed in the clinical pregnancy and implantation rates among the MC, KT, and Modified kit groups （37.2% vs. 30.2% vs. 39.6%; 21.9% vs. 18.8% vs. 27.4%, respectively）（P 〉 0.05）. The high-quality embryo rate per warmed oocyte was significantly higher （23.4%） in the Modified kit group than in the other groups （P 〈 0.001）. The embryo utilization and live birth rates per warmed oocyte were the highest in the Modified kit group, but not significantly （P 〉 0.05）. Conclusions： Modified vitrification media are efficient for oocyte vitrification and. with further verification, may be able to replace commercially available media in future clinical applications.

关键词： Fertilization Live Birth Oocytes Pregnancy Vitrification Media

Roles of insulin-like growth factor Ⅱ in regulating female reproductive physiology

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Science China(Life Sciences) 2020年第6期63卷 849-865页

作者： Tahir Muhammad Mengjing Li Jianfeng Wang Tao Huang Shigang Zhao Han Zhao Hongbin Liu Zi-Jiang Chen center for reproductive Medicine Cheeloo College of MedicineShandong UniversityJinan 250012China National Research Center for Assisted Reproductive Technology and Reproductive Genetics Shandong UniversityJinan 250012China Key laboratory of reproductive Endocrinology of Ministry of Education Shandong UniversityJinan 250012China Shandong Provincial Clinical Medicine research center for reproductive Health Shandong UniversityJinan 250012China Shanghai Key Laboratory for assisted Reproduction and reproductive genetics Shanghai 20000China center for reproductive Medicine Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200000 China

The number of growth factors involved in female fertility has been extensively studied, but reluctance to add essential growth factors in culture media has limited progress in optimizing embryonic growth and implantation outcomes, a situation that has ultimately led to reduced pregnancy outcomes. Insulin-like growth factor Ⅱ(IGF-Ⅱ) is the most intricately regulated of all known reproduction-related growth factors characterized to date, and is perhaps the predominant growth factor in human ovarian follicles. This review aims to concisely summarize what is known about the role of IGF-Ⅱ in follicular development, oocyte maturation, embryonic development, implantation success, placentation, fetal growth, and in reducing placental cell apoptosis, as well as present strategies that use growth factors in culture systems to improve the developmental potential of oocytes and embryos in different species. Synthesizing the present knowledge about the physiological roles of IGF-Ⅱ in follicular development, oocyte maturation, and early embryonic development should, on the one hand, deepen our overall understanding of the potential beneficial effects of growth factors in female reproduction and on the other hand support development(optimization) of improved outcomes for assisted reproductive technologies.

关键词： growth factors IGF-Ⅱ oocyte maturation embryonic development placentation fetal growth

A new TEX11 mutation causes azoospermia and testicular meiotic arrest

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Asian Journal of Andrology 2021年第5期23卷 510-515页

作者： Xiao-Chen Yu Meng-Jing Li Fei-Fei Cai Si-Jie Yang Hong-Bin Liu Hao-Bo Zhang center for reproductive Medicine Cheeloo College of MedicineShandong UniversityJinan 250012China Key Laboratory of reproductive Endocrinology of Ministry of Education Shandong UniversityJinan 250012China Shandong Key Laboratory of reproductive Medicine Jinan 250012China Shandong Provincial Clinical research center for reproductive Health Jinan 25CX312China National Research Center for Assisted Reproductive Technology and Reproductive Genetics Shandong UniversityJinan 250012China

There are many unknown genetic factors that lead to infertility in nonobstructive azoospermia men.Here,we performed whole-exome sequencing in blood samples obtained from 40 azoospermia patients with meiotic arrest and found a novel c.151_154del(p.D51fs)frame-shift mutation in exon 3 of the testis expressed 11(TEX11)gene in one patient.Sanger sequencing analysis of the patient and 288 fertile men was performed to validate the mutation.Immunohistochemical analysis showed TEX11 expression in late-pachytene spermatocytes and in round spermatids in fertile human testes.In contrast,testes of the patient with TEX11 mutation underwent meiotic arrest and lacked TEX11 expression.Western blotting of human embryonic kidney(HEK293)cells transfected with a vector for the p.D51fs TEX11 variant detected no TEX11 expression.In conclusion,we identified a novel frame-shift mutation in the TEX11 gene in an azoospermia patient,emphasizing that this gene should be included in genetic screening panels for the clinical evaluation of azoospermia patients.

关键词： male infertility meiotic arrest nonobstructive azoospermia testis expressed 11

子宫内膜不典型增生或复杂性增生不孕患者孕激素和IVF后的妊娠结局分析（英文）

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第11期18卷 1022-1025页

作者： Miao LI Jia-lun SONG Ying ZHAO She-ling WU Hong-bin LIU Rong TANG Lei YAN center for reproductive Medicine Shandong Provincial HospitalAffiliated to Shandong UniversityJinan 250012China National Research Center for Assisted Reproductive Technology and Reproductive Genetics Jinan 250012China The Key Laboratory of reproductive Endocrinology(Shandong University) Ministry of EducationJinan 250012China

本回顾性队列研究包括了年龄在45岁以下被诊断为伴有或不伴有不典型增生的子宫内膜复杂性增生患者,对孕激素治疗后的临床结局及妊娠结局进行了比较。结果表明,伴有或不伴有不典型增生的子宫内膜复杂性增生接受孕激素治疗后,其临床及妊... 详细信息

本回顾性队列研究包括了年龄在45岁以下被诊断为伴有或不伴有不典型增生的子宫内膜复杂性增生患者,对孕激素治疗后的临床结局及妊娠结局进行了比较。结果表明,伴有或不伴有不典型增生的子宫内膜复杂性增生接受孕激素治疗后,其临床及妊娠结局并没有显著差异。我们认为对于这些患者,在短暂的孕激素治疗控制病情后,尽快施行体外受精-胚胎移植治疗(IVF)将有利于改善其妊娠结局。

关键词：孕激素治疗子宫内膜增生体外受精-胚胎移植治疗(IVF)

Elective single blastocyst transfer is more suitable for normal responders than for high responders

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Chinese Medical Journal 2013年第11期126卷 2125-2128页

作者： WU Ke-liang ZHAO Hai-bin LIU Hui ZHONG Wan-xia YU Guan-ling CHEN Zi-jiang center for reproductive Medicine Provincial Hospital Affiliated to Shandong University National Research Center for Assisted Reproductive Technology and Reproductive Genetics China Key laboratory for Reproductive Endocrinology of Ministry of Education Shandong Provincial Key Laboratory of Reproductive Medicine Jinan Shandong 250021 China

Background Embryo quality and receptivity of the endometrium are two factors that determine the results of in vitro fertilization/intra-cytoplasmic sperm injection-embryo transfer （IVF/ICSI-ET）. There is no consensus of the optimal transfer strategy for normal responders or high responders. The current study aimed to find the optimal transfer strategy for different subgroups of patients. Methods From April 2010 to December 2010, patients who meet the following criteria were included in this study; primary infertility, female age 〈35 years, FSH level on female cycle day 2-3 〈12 mlU/ml, at least six good quality embryos available on day three.The clinical outcomes using different transfer strategies between normal responders and high responders were reviewed and compared. Results For the normal responders, the clinical pregnancy rate of day three double-embryo transfer （DET） was comparable to that of day five elective single blastocyst transfer （eSBT）, 64.04% vs. 60.33% （P〉0.05）. For the high responders, the clinical pregnancy rate of day five eSBT was significantly lower than that of day three DET, 43.35% vs. 57.21% （P〈0.05）. For the high responders, the rates of clinical pregnancy and implantation in frozen-thawed embryo transfer （FET） cycles were notably higher than in eSBT cycles （64.56% vs. 43.35% and 62.11% vs. 43.35% respectively）（P〈0.05）. Conclusions For normal responders, eSBT might be an applicabte strategy to reduce multiple pregnancy rates while maintaining acceptable overall pregnancy rates. And in order to reduce multiple pregnancies and increase the chance of pregnancy of high responders, FET may be a preferable strategy.

关键词： embryo transfer elective single blastocyst transfer high responder normal responder

Effect of orlistat on obese women with polycystic ovary syndrome

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Journal of Bio-X research 2018年第3期1卷 128-131页

作者： Qi Jiang Yuhua Shi center for reproductive Medicine Shandong UniversityNational Research Center for Assisted Reproductive Technology and Reproductive Geneticsthe Key Laboratory for Reproductive Endocrinology of Ministry of EducationShandong Provincial Clinical Medicine Research Center for Reproductive HealthJinanShandong ProvinceChina

Polycystic ovary syndrome(PCOS)is a common gynecological endocrine syndrome characterized by metabolic abnormality and reproductive dysfunction,including ovulation abnormality,hyperandrogenism and polycystic ovarian manifestations.Most PCOS patients are overweight or obese,particularly the central type.This aggravates the clinical manifestations of PCOS and ultimately increases the risk of infertility.Orlistat is the only weight loss drug approved by the US Food and Drug Administration.As a long-lasting gastrointestinal lipase inhibitor,orlistat can effectively reduce the absorption of lipid and increase lipid excretion,thereby achieving weight loss.In obese PCOS patients,orlistat can reduce weight,improve lipid metabolism and insulin resistance,improve sex hormone disorder,and have a positive effect on pregnancy outcome.This review summarizes the impact of orlistat treatment on metabolism and fertility in obese women with PCOS.

关键词： hyperandrogenism insulin resistance obesity orlistat polycystic ovary syndrome pregnancy outcome

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