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Emergence of Zika Virus in Culex tritaeniorhynchus and Anopheles sinensis Mosquitoes in China

Virologica Sinica 2021年第1期36卷 33-42页

作者： Jing Wang Hongbin Xu Song Song Rui Cheng Na Fan Shihong Fu Shaozai Zhang Ziqian Xu Ying He Wenwen Lei Fan Li Huanyu Wang Xiaoqing Lu Guodong Liang Qingdao University Qingdao 266071China State Key Laboratory of Infectious Disease prevention and control National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Jiangxi Province Center for Disease control and prevention Nanchang 330029China Qingdao Municipal Center for Disease control and prevention Qingdao 266071China Luoyang City Center for Disease control and prevention Luoyang 471000China

Zika virus(ZIKV)has been isolated from mosquitoes such as Aedes,Mansonia uniformis,and Culex perfuscus;However,the isolation of ZIKV from Anopheles sinensis and Culex tritaeniorhynchus has not yet been *** June and July2018,22,985 mosquitoes and 57,500 midges were collected in Jiangxi Province in southeastern *** them,six strains of ZIKV were isolated from mosquitoes:four from *** and two from *** genetic analysis showed that the ZIKV isolated from *** and *** belonged to genotype 2 in the Asian evolutionary branch of *** addition,the ZIKV strains isolated from *** and *** had amino acid substitutions identical to ZIKV strains prevalent in South America since *** study is the first to isolate ZIKV from mosquito specimens collected in the wild of Jiangxi Province,China;This is also the first time that ZIKV has been isolated from *** and *** that *** and *** have a very wide geographical distribution in China and even in eastern and southern Asia,the isolation of several strains of ZIKV from these two mosquitoes poses new challenges for the prevention and control of ZIKV infection in the mainland of China and countries and regions with the same distribution of mosquitoes.

关键词： Anopheles sinensis Culex tritaeniorhynchus Zika virus(ZIKV)

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A Nested-Polymerase Chain Reaction Assay to Identify and Genotype Brucella

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Biomedical and Environmental Sciences 2021年第3期34卷 227-231页

作者： TIAN Guo Zhong national Institute for Communicable Disease control and prevention Chinese Center for Disease Control and PreventionBeijing 102206China

Brucella is gram-negative,facultative,intracellular bacteria implicated in infectious zoonosis diseases,particularly among domestic animals,that are also transmittable to *** genus Brucella is classified based on the ... 详细信息

关键词： Chain Nested transmit

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A Case of Psittacosis-Qingdao City,Shandong Province,China,May 2023

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China CDC weekly 2023年第28期5卷 637-638页

作者： Ying Li Bei Pan Wenzhe Xiao Lizhu Fang Lijie Zhang Fachun Jiang Jing Jia Huilai Ma Chinese Field Epidemiology Training program Chinese Center for Disease Control and PreventionBeijingChina national Institute for Viral Disease control and prevention Chinese Center for Disease Control and PreventionBeijingChina Qingdao Municipal Center for Disease control and prevention Qingdao CityShandong ProvinceChina Jiaozhou Center for Disease control and prevention Qingdao CityShandong ProvinceChina

A case of psittacosis was identified in Qingdao City,Shandong Province on May 4,*** patient,a 69-year-old retired male,was admitted to a medical facility on April 22,*** symptoms,which had persisted for two days prior to his admission,included a fever(reaching 38.4°C at its peak),an escalating cough,and wheezing,none of which had been previously treated.

关键词： admitted Qingdao Shandong

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Full-Length Genome Sequencing of SARS-CoV-2 Directly from Clinical and Environmental Samples Based on the Multiplex Polymerase Chain Reaction Method

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Biomedical and Environmental Sciences 2021年第9期34卷 725-728页

作者： NIU Pei Hua ZHAO Xiang LU Rou Jian ZHAO Li HUANG Bao Ying YE Fei WANG Da Yan TAN Wen Jie national Institute for Viral Disease control and prevention Chinese Center for Disease Control and PreventionBeijing 102206China

The new coronavirus,severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),is spreading worldwide with the number of confirmed cases increasing dramatically[1,2].Coronaviruses have the largest genome among RNA vi... 详细信息

关键词： acute respiratory Clinical

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Food Frequency Questionnaire for Chinese Children Aged 12-17 Years: Validity and Reliability

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Biomedical and Environmental Sciences 2019年第7期32卷 486-495页

作者： LIU Dan JU La Hong YANG Zhen Yu ZHANG Qian GAO Jian Fen GONG Di Ping GUO Dan Dan LUO Shu Quan ZHAO Wen Hua national Institute for Nutrition and Health Chinese Center for Disease Control and Prevention Beijing 100050 China Yanqing Center for Disease control and prevention Beijing 102100 China Wanzhou Center for Disease control and prevention Chongqing 404000 China Beijing Center for Disease prevention and control Beijing 100013 China Chongqing Center for Disease control and prevention Chongqing 400042 China

Objective The primary objective of this study was to examine the validity and reliability of a semi-quantitative food frequency questionnaire(FFQ) among Chinese children aged 12-17 years. Methods A semi-quantitative 72-food item FFQ was developed for children aged 12-17 years. The reliability and validity of this FFQ were evaluated against 24-h dietary recalls(24 h DRs) to measure the consumption of foods and nutrients. We administered two FFQs and three DRs to children(N = 160) over a period of 1 month to evaluate the reliability and validity. Reliability was examined by quartile agreement and intraclass correlation coefficients(ICCs), and validity was examined by quartile agreement, Bland-Altman plots and correlation with DRs. Results For reliability, the ICCs between the two FFQs ranged from 0.21 to 0.76 for foods and nutrients, and the quartile agreement ranged from 70.0% to 95.0% in the same or adjacent quartiles. Spearman’s correlation coefficients of foods and nutrients between the second FFQ and the 24 h DRs ranged from-0.04 to 0.59. The Bland-Altman plots demonstrated good agreement across the range of intakes among nutrients. The quartile agreement ranged from 50.0% to 100.0%, with infrequent misclassification. Conclusion The FFQ assessment of dietary intakes demonstrated acceptable relative validity and high reproducibility for Chinese children aged 12-17 years.

关键词： Reliability Validity Food frequency questionnaire Chinese children

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Childhood BMI and Adult Obesity in a Chinese Sample:A 13-Year Follow-up Study

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Biomedical and Environmental Sciences 2019年第3期32卷 162-168页

作者： LIU Dan HAO Yun Xia ZHAO Ting Zhi SONG Peng Kun ZHAI Yi PANG Shao Jie ZHAO Yan Fang ZHANG Mei WANG Zhuo Qun MI Sheng Quan WANG Yu Ying ZHANG Jian ZHAO Wen Hua national Institute for Nutrition and Health Chinese Center for Disease Control and Prevention Yangcheng Center for Disease control and prevention Heshun Center for Disease control and prevention Beijing Tian Tan Hospital Capital Medical University Academy of State Administration of Grain national Center for Chronic and Non-Communicable Diseases control and prevention Chinese Center for Disease Control and Prevention College of Biochemical Engineering Beijing Union University International Life Sciences Institute Focal Point in China Chinese Center for Disease control and prevention

Objective Obesity is recognized as a significant risk factor for diabetes and hypertension. The present study aimed to examine the associations between adults' obesity risk and childhood and parental obesity. Methods A total of 204 children aged 6-17 years were recruited in 2002 with an average follow-up period of 13.2 years. Height and body weight were measured by trained staffs. Overweight and obesity were defined based on the Chinese standard for children and adults. T-test, analysis of variance, and Chi-square analysis were used for single factor analysis. Multiple linear and logistic regression analyses were used to perform multifactor analysis. Results The percentage of non-obese children who grew up to be non-obese adults was 62.6%, and that of obese children who grew up to be obese adults was 80.0%. There was a significant association between childhood body mass index(BMI) and adulthood BMI with a β regression coefficient of 3.76 [95% confidence interval(CI): 1.36-6.16], and between childhood obesity and adulthood obesity with an odds ratio of 5.76(95% CI: 1.37-24.34). There was no statistical difference between parental obesity at baseline and children's adulthood obesity, after adjustment of confounders. Male participants and those aged 10.0-13.0 years had a higher risk of adulthood obesity with odds ratios of 2.50(95% CI: 1.12-5.26) and 3.62(95% CI: 1.17-11.24), respectively. Conclusion Childhood obesity is an important predictor of adulthood obesity.

关键词： Obesity Children and adolescents Life course epidemiology Cohort study China

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Viral and Bacterial Etiology of Acute Febrile Respiratory Syndrome among Patients in Qinghai, China

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Biomedical and Environmental Sciences 2019年第6期32卷 438-445页

作者： LIU Gao Shan LI Hong ZHAO Sheng Cang LU Rou Jian NIU Pei Hua TAN Wen Jie NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China Center for Disease control and prevention of Qinghai Province Xining 810007 Qinghai China

Objective This study was conducted to investigate the viral and bacterial etiology and epidemiology of patients with acute febrile respiratory syndrome(AFRS) in Qinghai using a commercial routine multiplex-ligation-nucleic acid amplification test(NAT)-based assay. Methods A total of 445 nasopharyngeal swabs specimens from patients with AFRS were analyzed using the RespiFinderSmart22 kit(PathoFinder BV, Netherlands) and the LightCycler 480 real-time PCR system. Results Among the 225(225/445, 51%) positive specimens, 329 positive pathogens were detected, including 298(90.58%) viruses and 31(9%) bacteria. The most commonly detected pathogens were influenza virus(IFV;37.39%;123/329), adenovirus(AdV;17.02%;56/329), human coronaviruses(HCoVs;10.94%;36/329), rhinovirus/enterovirus(RV/EV;10.03%;33/329), parainfluenza viruses(PIVs;8.51%;28/329), and Mycoplasma pneumoniae(M. pneu;8.51%;28/329), respectively. Among the co-infected cases(17.53%;78/445), IFV/AdV and IFV/M. pneu were the most common co-infections. Most of the respiratory viruses were detected in summer and fall. Conclusion In our study, IFV-A was the most common respiratory pathogen among 22 detected pathogens, followed by AdV, HCoV, RV/EV, PIV, and M. pneu. Bacteria appeared less frequently than viruses, and co-infection was the most common phenomenon among viral pathogens. Pathogens were distributed among different age groups and respiratory viruses were generally active in July, September, and November. Enhanced surveillance and early detection can be useful in the diagnosis, treatment, and prevention of AFRS, as well as for guiding the development of appropriate public health strategies.

关键词： Acute febrile respiratory syndrome Viral and bacterial etiology Prevalence Seasonal distribution

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Acute Effects of Low Temperatures and Cold Waves on Elderly Infectious Pneumonia Mortality—Jinan City,Shandong Province,China,2014-2022

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China CDC weekly 2024年第5期6卷 77-82,I0002-I0005页

作者： Huiyun Chang Mingjun Li Ying Wang Liangliang Cui Tiantian Li Jinan Municipal Center for Disease control and prevention Jinan Municipal Center for Disease Control and Prevention Affiliated to Shandong UniversityJinan CityShandong ProvinceChina Chinese Filed Epidemiology Training program Chinese Center for Disease Control and PreventionBeijingChina China CDC Key Laboratory of Environment and Population Health National Institute of Environmental HealthChinese Center for Disease Control and PreventionBeijingChina

Summary What is already known about this topic?The mortality rate due to pneumonia varies depending on the infectious agents present in a low-temperature *** is added by this report?This study aimed to examine the relationship between low temperatures and cold waves and the risk of mortality from infectious pneumonia in the *** findings indicate a significant increase in the risk of infectious pneumonia.

关键词： environment Shandong finding

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Polyethylene glycol fusion repair of severed rat sciatic nerves reestablishes axonal continuity and reorganizes sensory terminal fields in the spinal cord

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Neural Regeneration Research 2025年第7期20卷 2095-2107页

作者： Emily A.Hibbard Liwen Zhou Cathy Z.Yang Karthik Venkudusamy Yessenia Montoya Alexa Olivarez George D.Bittner Dale R.Sengelaub program in Neuroscience Indiana UniversityBloomingtonINUSA Department of Neuroscience University of Texas at AustinAustinTXUSA

Peripheral nerve injuries result in the rapid degeneration of distal nerve segments and immediate loss of motor and sensory functions;behavioral recovery is typically *** used a plasmalemmal fusogen,polyethylene glycol(PEG),to immediately fuse closely apposed open ends of severed proximal and distal axons in rat sciatic *** have previously reported that sciatic nerve axons repaired by PEG-fusion do not undergo Wallerian degeneration,and PEG-fused animals exhibit rapid(within 2–6 weeks)and extensive locomotor ***,our previous report showed that PEG-fusion of severed sciatic motor axons was non-specific,i.e.,spinal motoneurons in PEG-fused animals were found to project to appropriate as well as inappropriate target *** this study,we examined the consequences of PEG-fusion for sensory axons of the sciatic *** adult male and female rats(Sprague–Dawley)received either a unilateral single cut or ablation injury to the sciatic nerve and subsequent repair with or without(Negative control)the application of *** action potentials recorded immediately after PEG-fusion repair confirmed conduction across the injury *** success of PEG-fusion was confirmed through Sciatic Functional Index testing with PEG-fused animals showing improvement in locomotor function beginning at 35 days *** 2–42 days postoperatively,we anterogradely labeled sensory afferents from the dorsal aspect of the hindpaw following bilateral intradermal injection of wheat germ agglutinin conjugated horseradish ***-fusion repair reestablished axonal *** to unoperated animals,labeled sensory afferents ipsilateral to the injury in PEG-fused animals were found in the appropriate area of the dorsal horn,as well as inappropriate mediolateral and rostrocaudal ***,despite having intact peripheral nerves,similar reorganizations of labeled sensory afferents were also observed contralateral to the injury and repa

关键词： axotomy dorsal horn peripheral nerve injury plasticity polyethylene glycol(PEG) sciatic nerve sensory terminals wheat germ agglutinin horseradish peroxidase

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An Outbreak of Serogroup Y Meningococcal Meningitis in a Private Secondary Vocational School-Guangzhou City,Guangdong Province,China,2023

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China CDC weekly 2024年第12期6卷 242-246,I0005页

作者： Xiang Zeng Qing He Yilan Li Yong Zhou Xiaoping Shao Pei Hu Jian Liang Yudan Song Chao Ma Lijie Zhang Limei Sun Lei Luo Chinese Field Epidemiology Training program Chinese Center for Disease Control and PreventionBeijingChina Zhuhai Center for Disease control and prevention Zhuhai CityGuangdong ProvinceChina Guangdong Center for Disease control and prevention Guangzhou CityGuangdong ProvinceChina Guangzhou Center for Disease control and prevention Guangzhou CityGuangdong ProvinceChina Institute of Public Health Guangzhou Medical University&Guangzhou Center for Disease Control and PreventionGuangzhou CityGuangdong ProvinceChina Department of the national Immunization programme Chinese Center for Disease Control and PreventionBeijingChina

What is already known about this topic?The inclusion of meningococcal vaccines in the national Immunization program(NIP)over several years has significantly reduced the incidence of meningococcal meningitis in China to historic ***,there has been a diversification of meningococcal serogroups,leading to a shift in dominant serogroups in China from serogroup A to serogroups C and B,accompanied by a rise in reports of serogroups Y and *** is added by this report?An outbreak of serogroup Y Neisseria meningitidis(Nm)in a secondary vocational school involved a single confirmed severe case and 24 individuals with laboratory-confirmed Nm *** investigation revealed that the outbreak was localized to the classroom of the confirmed *** close contact within a confined space was identified as a significant risk factor for Nm *** genotype sequence identified was type 1655(ST-1655),which is categorized under clonal complex 23(CC-23)and bears resemblance to 8 previously confirmed cases of serogroup Y meningococcal meningitis within Guangdong *** suggests that serogroup Y infections continue to sporadically emerge and have become prevalent *** are the implications for public health practice?This outbreak underscores the critical need to enhance surveillance of meningococcal serogroups and population carrier,and advocate for vaccination with MenY-containing vaccines.

关键词： break Secondary Guangdong

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