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A molecular probe carrying anti-tropomyosin 4 for early diagnosis of cerebral ischemia/reperfusion injury

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Neural Regeneration Research 2023年第6期18卷 1321-1324页

作者： Teng-Fei Yu Kun Wang Lu Yin Wen-Zhe Li Chuan-Ping Li Wei Zhang Jie Tian Wen He department of Ultrasound Beijing Tiantan HospitalCapital Medical UniversityBeijingChina CAS key laboratory of molecular Imaging Institute of AutomationChinese Academy of SciencesBeijingChina State key laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijingChina Anhui Province key laboratory of Functional Coordinated Complexes for Materials Chemistry and Application School of Chemical and Environmental EngineeringAnhui Polytechnic UniversityWuhuAnhui ProvinceChina Beijing Advanced Innovation Center for Big Data-Based Precision Medicine School of Engineering MedicineBeihang UniversityBeijingChina

In vivo imaging of cerebral ischemia/reperfusion injury remains an important challenge.We injected porous Ag/Au@SiO_(2) bimetallic hollow nanoshells carrying anti-tropomyosin 4 as a molecular probe into mice with cerebral ischemia/reperfusion injury and observed microvascular changes in the brain using photoacoustic imaging with ultrasonography.At each measured time point,the total photoacoustic signal was significantly higher on the affected side than on the healthy side.Twelve hours after reperfusion,cerebral perfusion on the affected side increased,cerebrovascular injury worsened,and anti-tropomyosin 4 expression increased.Twenty-four hours after reperfusion and later,perfusion on the affected side declined slowly and stabilized after 1 week;brain injury was also alleviated.Histopathological and immunohistochemical examinations confirmed the brain injury tissue changes.The nanoshell molecular probe carrying anti-tropomyosin 4 has potential for use in early diagnosis of cerebral ischemia/reperfusion injury and evaluating its progression.

关键词： cerebral ischemia/reperfusion injury diagnosis dynamic monitoring ischemic stroke middle cerebral artery occlusion molecular probe nanoshells photoacoustic imaging tropomyosin 4 ultrasound

Self-assembled Conformations of a Core-shell Comb-like Chain with Adjustable Architectural Parameters

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Chinese Journal of Polymer Science 2023年第9期41卷 1439-1446,I0008页

作者： Huan-Li Qi Heng-Wei Zhou Chao Duan Wei-Hua Li Ming-Ming Ding Xinjiang laboratory of Phase Transitions and Microstructures in Condensed Matter Physics College of Physical Science and TechnologyYili Normal UniversityYining835000China State key laboratory of molecular Engineering of Polymers Department of Macromolecular ScienceFudan UniversityShanghai200433China School of Chemical Engineering and Light Industry Guangdong University of TechnologyGuangzhou510006China

Combining Brownian dynamics simulations and self-consistent field theory,we demonstrate that the architectural parameters of coreshell comb-like chains have a decisive influence on their final self-assembled conformations.When the ratio of hydrophilic and hydrophobic segments is N_(g,g)∶N_(g,r)=3∶1,no core-shell structure is observed,and unsegregated chains or clusters are substituted.When N_(g,g)∶N_(g,r)=3∶2or 3∶3,the core-shell comb-like chains can be assembled into single micelles or structures where several small micelles are strung together by the backbone,which is similar to the pearl-necklace structure formed by the polyelectrolytes in poor solvent.With the increase of backbone length or grafting density,the probability of forming single micelles becomes lower,but the structures of two,three or more small micelles strung together are more observed.Our results indicate how to obtain the desired self-assembled structures of core-shell comb-like chains by regulating the architectural parameters,which could provide insights for the optimization of molecular design in various applications.

关键词： Comb-like polymer Self-assembled conformations Brownian dynamics simulations

Organophosphine ligand derived sandwich-structural electrocatalyst for oxygen evolution reaction

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Journal of Energy Chemistry 2022年第7期31卷 74-83,I0003页

作者： Zejun Sun Wenjuan Xu Liutao Guo Qiang Han Jianyi Gao Jiaping Wang Yanru Feng Chengrui Li Qionglin Liang Hong-bin Sun Yang Yang department of Chemistry Northeastern UniversityShenyang 110819LiaoningChina key laboratory of Bioorganic Phosphorus Chemistry&Chemical biology(Ministry of Education) Department of ChemistryCenter for Synthetic and Systems BiologyTsinghua UniversityBeijing 100084China Astronaut Centre of China Beijing 100094China Nano Science Technology Center Department of Materials Science and EngineeringDepartment of ChemistryRenewable Energy and Chemical Transformation Cluster University of Central FloridaOrlandoFL 32826United States

The synchronous construction of metal phosphate and phosphorus-doped carbon structures is of great significance to innovate the design,synthesis,and application of catalysts,as these phosphoruscontaining composite materials have shown a remarkable contribution to electrocatalysts.However,their preparation procedure generally involves using large amounts of excess phosphorus sources for phosphorization,which inevitably release poisonous PH_(3) or dangerous phosphorus vapor.Here,a strategy for in-situ formation of FePO_(4) embedded in P-doped carbon 2D nano film(FePO_(4)/PdC)is developed using a highly integrated precursor,which is a small molecular organophosphine ligand,1,1’bis(diphenylphosphine)ferrocene(DPPF).The multi-source precursor DPPF that contains Fe,P,and C is molecular-vapor-deposited on the nickel foam(NF)supported ZIF-67 nanosheets to obtain the composite catalyst,namely DPPF-500/ZIF-67/NF.FePO_(4)/PdC encapsulated the ZIF-67 derived Co/N-doped carbon matrix(Co NC)to form a sandwich structure FePO_(4)/PdC@CoNC.The constructed catalyst shows good performance for OER,requiring an overpotential of only 297 m V to deliver 600 m A/cm^(2) with a Tafel slope of 42.7 m V dec^(-1).DFT calculations demonstrate that the synergistic effects between the metal active center and P-doped carbon film reduce the energy barriers and improve electron transport.This method of constructing P-containing catalysts overcomes the demand for additional P sources to realize eco-friendly fabrication and yields a unique structure with good catalytic activity.

关键词： Organophosphorus ligand Synchronous Synthesis FePO_(4) P-doped Carbon 2D nano film Oxygen evolution reaction

Development and application of bionic systems consisting of tumor-cell membranes

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2022年第9期23卷 770-777页

作者： Tianjiao PENG Jun YAO Clinical Medical College Henan University of Science and TechnologyLuoyang471003China department of Oncology the First Affiliated Hospital of Henan University of Science and TechnologyLuoyang471003China Epigenetics and molecular biology laboratory the First Affiliated Hospital of Henan University of Science and TechnologyLuoyang471003China

Malignant tumors pose a serious threat to human health but during the past decade,great progress has been made in the treatment of tumors.The tumorcell membrane is well constructed and can be used to solve problems in tumor therapy.Tumor-cell membranes exhibit not only high biocompatibility duc to their homology but also enhanced therapeutic ffects when combined with nanotechnology.Meanwhile,nanoma-tcrials show high selectivity,sensitivity,and clinical transformation potential.Enhanced immunotherapy or tumor vaccines have potential clinical application because of tumor-membranc surfacc-specific antigens.Several studies have confirmed the feasibility and ad-vantages of using tumorcell membrane incorporated nanosystems for tumor therapy.Considering all this,we focus in this review on the application of tumor-cell-membrane bionic platforms and,in the summary,provide ideas for new scientific developments.

关键词： membranes tumor clinical

Transcription factors AS1 and AS2 interact with LHP1 to repress KNOX genes in Arabidopsis

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Journal of Integrative Plant biology 2016年第12期58卷 959-970页

作者： Zhongfei Li Bin Li Jian Liu Zhihao Guo Yuhao Liu Yan Li Wen-Hui Shen Ying Huang Hai Huang Yijing Zhang Aiwu Dong State key laboratory of Genetic Engineering International Associated Laboratory of CNRS-Fudan-HUNAU on Plant Epigenome Research Collaborative Innovation Center for Genetics and Development Institute of Plant Biology School of Life Sciences Fudan University Shanghai 200433 China national laboratory of Plant molecular Genetics Shanghai Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China national Center for Protein Science Shanghai State Key Laboratory of Molecular Biology Shanghai State Key Laboratory of Molecular Andrology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China Institut de Biologie Moléculaire des Plantes du CNRS Université de Strasbourg 12 rue du General Zimmer 67084 Strasbourg Cedex France

Polycomb group proteins are important repressors of numerous genes in higher eukaryotes. However, the mechanism by which Polycomb group proteins are recruited to specific genes is poorly understood. In Arabidopsis, LIKE HETEROCHROMATIN PROTEIN 1 （LHP1）, also known as TERMINAL FLOWER 2, was originally proposed as a subunit of polycomb repressive complex 1 （PRC1） that could bind the tri-methylated lysine 27 of histone H3 （H3K27me3） established by the PRC2. In this work, we show that LHP1 mainly functions with PRC2 to establish H3K27me3, but not with PRC1 to catalyze monoubiquitination at lysine 119 of histone H2A. Our results show that complexes of the transcription factors ASYMMETRIC LEAVES 1 （AS1） and AS2 could help to establish the H3K27me3 modification at the chromatin regions of Class-I KNOTTED＇t-like homeobox （KNOX） genes BREVIPEDICELLU5 and KNAT2 via direct interactions with LHP1. Additionally, our transcriptome analysis indicated that there are probably more common target genes of AS1 and LHP1 besides Class-I KNOX genes during leaf development in Arabidopsis.

关键词： Arabidopsis thaliana ASYMMETRIC LEAVES 1

Targeting PSAT1 to mitigate metastasis in tumors with p53-72Pro variant

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Signal Transduction and Targeted Therapy 2023年第3期8卷 1237-1250页

作者： Jingwen Jiang Hai-Ning Chen Ping Jin Li Zhou Liyuan Peng Zhao Huang Siyuan Qin Bowen Li Hui Ming Maochao Luo Na Xie Wei Gao Edouard C.Nice Qiang Yu Canhua Huang State key laboratory of Biotherapy and Cancer Center West China HospitalSichuan Universityand Collaborative Innovation Center for BiotherapyChengdu 610041P.R.China West China School of Basic Medical Sciences&Forensic Medicine Sichuan UniversityChengdu 610041P.R.China Colorectal Cancer Center Department of General SurgeryState Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan UniversityChengdu 610041P.R.China department of Biochemistry and molecular biology Monash UniversityClaytonVICAustralia Cancer Precision Medicine Genome Institute of SingaporeAgency for ScienceTechnologyand ResearchBiopolisSingapore 138672Singapore

The single-nucleotide polymorphism(SNP)of p53,in particular the codon 72 variants,has recently been implicated as a critical regulator in tumor progression.However,the underlying mechanism remains elusive.Here we found that cancer cells carrying codon 72-Pro variant of p53 showed impaired metastatic potential upon serine supplementation.Proteome-wide mapping of p53-interacting proteins uncovered a specific interaction of the codon 72 proline variant(but not p5372R)with phosphoserine aminotransferase 1(PSAT1).Interestingly,p53^(72P)-PSAT1 interaction resulted in dissociation of peroxisome proliferator-activated receptor-γcoactivator 1α(PGC-1α)that otherwise bound to p53^(72P),leading to subsequent nuclear translocation of PGC-1αand activation of oxidative phosphorylation(OXPHOS)and tricarboxylic acid(TCA)cycle.Depletion of PSAT1 restored p53^(72P)-PGC-1αinteraction and impeded the OXPHOS and TCA function,resulting in mitochondrial dysfunction and metastasis suppression.Notably,pharmacological targeting the PSAT1-p53^(72P)interaction by aminooxyacetic acid(AOA)crippled the growth of liver cancer cells carrying the p53^(72P)variant in both in vitro and patient-derived xenograft models.Moreover,AOA plus regorafenib,an FDA-proved drug for hepatocellular carcinoma and colorectal cancer,achieved a better anti-tumor effect on tumors carrying the p53^(72P)variant.Therefore,our findings identified a gain of function of the p53^(72P)variant on mitochondrial function and provided a promising precision strategy to treat tumors vulnerable to p53^(72P)-PSAT1 perturbation.

关键词： metastasis impaired tumors

通过给桑蚕喂食稀土离子改性的饲料制备高强高韧的蚕丝纤维

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Science Bulletin 2023年第23期68卷 2973-2981,M0005页

作者：陆浩杰蹇木强干林丽张勇李硕梁晓平王灏珉朱梦嘉张莹莹 key laboratory of Organic Optoelectronics and molecular Engineering of Ministry of Education Department of ChemistryTsinghua UniversityBeijing 100084China Beijing Graphene Institute Beijing 100095China

蚕丝纤维具有优异的生物相容性和良好的力学性能,但以简单且大规模的方式生产强韧的蚕丝纤维仍是一项重大挑战.本文报道了一种通过给桑蚕喂食稀土离子改性桑叶饲料以制备强韧蚕丝纤维的方法.所得蚕丝纤维具有显著提高的拉伸强度和韧性,... 详细信息

蚕丝纤维具有优异的生物相容性和良好的力学性能,但以简单且大规模的方式生产强韧的蚕丝纤维仍是一项重大挑战.本文报道了一种通过给桑蚕喂食稀土离子改性桑叶饲料以制备强韧蚕丝纤维的方法.所得蚕丝纤维具有显著提高的拉伸强度和韧性,其平均值分别为0.85±0.07 GPa和156±13 MJ m^(-3),且最大值达到0.97±0.04 GPa和188±19 MJ m^(-3),这些性能接近蜘蛛牵引丝(自然界最强的丝纤维之一)的拉伸强度和韧性.研究结果表明,稀土离子(La^(3+)或En^(3+))成功掺入蚕丝纤维并与其形成相互作用.稀土离子减小了蚕丝纤维的直径,降低了其内部结晶组分的含量并提升了取向度.这些相互作用与结构演变同时提升了蚕丝纤维的强度和韧性.该研究提出了一种简单、可扩展且有效的生产超强韧丝纤维的策略,制备的蚕丝纤维有望应用于个人防护和航空航天等需要轻质结构材料的领域.

关键词： Silkworm silk Super strong and tough High orientation Low crystallinity Rare earth ions

molecular mechanisms of adaptive evolution in wild animals and plants

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Science China(Life Sciences) 2023年第3期66卷 453-495页

作者： Yibo Hu Xiaoping Wang Yongchao Xu Hui Yang Zeyu Tong Ran Tian Shaohua Xu Li Yu Yalong Guo Peng Shi Shuangquan Huang Guang Yang Suhua Shi Fuwen Wei CAS key Lab of Animal Ecology and Conservation biology Chinese Academy of SciencesBeijing 100101China State key laboratory for Conservation and Utilization of Bio-Resources in Yunnan School of Life SciencesYunnan UniversityKunming 650091China State key laboratory of Systematic and Evolutionary Botany Institute of BotanyChinese Academy of SciencesBeijing 100093China State key laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunming 650201China Institute of Evolution and Ecology School of Life SciencesCentral China Normal UniversityWuhan 430079China College of Life Sciences Nanjing Normal UniversityNanjing 210023China State key laboratory of Biocontrol Guangdong Key Lab of Plant ResourcesSchool of Life SciencesSun Yat-sen University Southern Marine Science and Engineering Guangdong laboratory(Guangzhou) Guangzhou 511458China

Wild animals and plants have developed a variety of adaptive traits driven by adaptive evolution,an important strategy for species survival and persistence.Uncovering the molecular mechanisms of adaptive evolution is the key to understanding species diversification,phenotypic convergence,and inter-species interaction.As the genome sequences of more and more non-model organisms are becoming available,the focus of studies on molecular mechanisms of adaptive evolution has shifted from the candidate gene method to genetic mapping based on genome-wide scanning.In this study,we reviewed the latest research advances in wild animals and plants,focusing on adaptive traits,convergent evolution,and coevolution.Firstly,we focused on the adaptive evolution of morphological,behavioral,and physiological traits.Secondly,we reviewed the phenotypic convergences of life history traits and responding to environmental pressures,and the underlying molecular convergence mechanisms.Thirdly,we summarized the advances of coevolution,including the four main types:mutualism,parasitism,predation and competition.Overall,these latest advances greatly increase our understanding of the underlying molecular mechanisms for diverse adaptive traits and species interaction,demonstrating that the development of evolutionary biology has been greatly accelerated by multi-omics technologies.Finally,we highlighted the emerging trends and future prospects around the above three aspects of adaptive evolution.

关键词： adaptive evolution adaptive trait coevolution comparative genomics convergent evolution genetic convergence molecular mechanism mutualism parasitism phenotype convergence

PDX1.1-dependent biosynthesis of vitamin B6 protects roots from ammonium-induced oxidative stress

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molecular Plant 2022年第5期15卷 820-839页

作者： Ying Liu Rodolfo A.Maniero Ricardo F.H.Giehl Michael Melzer Priscille Steensma Gabriel Krouk Teresa B.Fitzpatrick Nicolaus von Wirén molecular Plant Nutrition Leibniz Institute of Plant Genetics and Crop Plant Research(IPK)Corrensstrasse 306466 GaterslebenGermany Structural Cell biology Leibniz Institute of Plant Genetics and Crop Plant Research(IPK)Corrensstrasse 306466 GaterslebenGermany department of Botany and Plant biology University of Geneva1211 GenevaSwitzerland BPMP Universitéde MontpellierCNRSINRASupAgroMontpellierFrance State key laboratory of Crop Genetics and Germplasm Enhancement College of Resources and Environmental SciencesNanjing Agricultural UniversityNanjing 210095China

Despite serving as a major inorganic nitrogen source for plants,ammonium causes toxicity at elevated con-centrations,inhibiting root elongation early on.While previous studies have shown that ammonium-inhibited root development relates to ammonium uptake and formation of reactive oxygen species(ROS)in roots,it remains unclear about the mechanisms underlying the repression of root growth and how plants cope with this inhibitory effect of ammonium.In this study,we demonstrate that ammonium-induced apo-plastic acidification co-localizes with Fe precipitation and hydrogen peroxide(H_(2)O_(2))accumulation along the stele of the elongation and differentiation zone in root tips,indicating Fe-dependent ROS formation.By screening ammonium sensitivity in T-DNA insertion lines of ammonium-responsive genes,we identified PDX1.1,which is upregulated by ammonium in the root stele and whose product catalyzes de novo biosyn-thesis of vitamin B6.Root growth of pdx1.1 mutants is hypersensitive to ammonium,while chemical complementation or overexpression of PDX1.1 restores root elongation.This salvage strategy requires non-phosphorylated forms of vitamin B6 that are able to quench ROS and rescue root growth from ammo-nium inhibition.Collectively,these results suggest that PDX1.1-mediated synthesis of non-phosphorylated B6 vitamers acts as a primary strategy to protect roots from ammonium-dependent ROS formation.

关键词： ammonium nutrition apoplastic pH Fe mobilization root elongation pyridoxine ROS scavenging