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Joint deletion of multifunctional MGF505-7R and H240R genes generates a safe and effective African swine fever virus attenuated live vaccine candidate

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Virologica Sinica 2024年第3期39卷 355-357页

作者： Changjiang Weng Li Huang Guangqiang Ye Division of Fundamental Immunology National African Swine Fever Para-reference LaboratoryState Key Laboratory for Animal Disease Control and PreventionHarbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China Heilongjiang Provincial Key laboratory of Veterinary Immunology Harbin 150069China

African swine fever(ASF)caused by the ASF virus(ASFV)is a highly contagious disease of pigs and wild ***,there are no safe,effective commercial vaccines *** genome of ASFV HLJ/18 contains more than 180 genes,including a few virulence-related *** these key virulence genes through individual deletion and subsequent pathogenicity testing in pigs is a laborious *** addition,some ASFV genes are indispensable for viral replication and cannot be *** the past 30 years,scientists have confirmed that certain ASFV genes particularly those host antiviral innate immune responses,are associated with the virulence of ASFV *** knockout of these genes does not impact viral replication,it does attenuate the virulence of ASFV in *** immunized with these live attenuated vaccine candidates can provide partial or complete *** on these findings,we propose a new concept that ASFV immunomodulatory genes involved in type I interferon(IFN)production,IFN-JAK-STAT signaling,inflammatory responses,cell death(involved in apoptosis,necrosis,and pyroptosis),and autophagy may be related to the pathogenicity of *** unbiased screening may identify more ASFV immunomodulatory or multifunctional genes,simplifying the confirmation of ASFV virulence-related *** line with these theories,we have identified MGF505-7R and H240R as key virulence genes determining the pathogenicity of the ASFV HLJ/18 *** of MGF505-7R,H240R alone or both attenuate the virulence of ASFV HLJ/18 in pigs,providing a paradigm for developing attenuated live vaccines from basic research results.

关键词： attenuated fever swine

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SARS-CoV-2 Surveillance Through China Influenza Surveillance Information System—China,December 1,2022 to February 12,2023

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China CDC weekly 2023年第7期5卷 152-158页

作者： Xiaoxu Zeng Yiran Xie Xiaokun Yang Zhibin Peng Jing Tang Lei Yang Shuxia Lin Jun Han Yanping Zhang Dayan Wang national Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention/WHO Collaborating Centre for Reference and Research on InfluenzaBeijing MunicipalityChina Chinese Center for Disease Control and Prevention Beijing MunicipalityChina Key laboratory for Medical Virology National Health CommissionBeijing MunicipalityChina

Introduction:The World Health Organization(WHO)proposed using influenza surveillance systems to carry out coronavirus disease 2019(COVID-19)surveillance due to the similarity between the two diseases in some respiratory *** assess the prevalence of COVID-19,we analyzed the influenzalike illness(ILI)and positive rate of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)detections in ILI patients reported to the influenza Surveillance Information System(CNISIS)since late ***:Data related to ILI were reported by national surveillance sentinel *** testing for SARS-CoV-2 and influenza viruses was conducted using real-time reverse transcription polymerase chain reaction(rRT-PCR)detection by the national influenza surveillance network *** data were reported to ***:Beginning on December 12,2022(Week 50),the ILI percentage increased dramatically,peaking in Week 51 at 12.1%.Subsequently,the ILI percentage began to decline rapidly from Week 52,2022,and by Week 6,2023(February 6-12),the ILI and ILI percentage had returned to the levels observed at the beginning of December *** December 1,2022 to February 12,2023,115,844 specimens were tested for both SARS-CoV-2 and influenza *** these,30,381(26.2%)were positive for SARSCoV-2 and 1,763(1.5%)were positive for influenza *** positive rate of SARS-CoV-2 tests peaked at 74.1%around December 23 and ***:Sentinel-based surveillance,previously established for influenza,is an effective way to track the circulation trend of SARS-CoV-2 during community-level *** was no coprevalence of SARS-CoV-2 and influenza virus during the outbreak of SARS-CoV-2,even during the winter influenza ***,it is important to remain vigilant for the potential rise of influenza activities following the COVID-19 epidemic.

关键词： winter December surveillance

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Porcine epidemic diarrhea virus nsp14 inhibits NF-κB pathway activation by targeting the IKK complex and p65

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Animal Diseases 2021年第3期1卷 162-172页

作者： Shasha Li Fan Yang Caina Ma Weijun Cao Jinping Yang Zhenxiang Zhao Hong Tian Zixiang Zhu Haixue Zheng State Key laboratory of Veterinary Etiological Biology National Foot and Mouth Diseases Reference LaboratoryKey Laboratory of Animal Virology of Ministry of AgricultureLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhouGansu 730046China

Coronaviruses(CoVs)are a group of related enveloped RNA viruses that have severe consequences in a wide variety of animals by causing respiratory,enteric or systemic *** epidemic diarrhea virus(PEDV)is an economically important CoV distributed worldwide that causes diarrhea in ***14 is a nonstructural protein of PEDV that is involved in regulation of innate immunity and viral ***,the function and mechanism by which nsp14 modulates and manipulates host immune responses remain largely ***,we report that PEDV nsp14 is an NF-κB pathway *** PEDV nsp14 protein remarkably decreases SeV-,poly(I:C)-and TNF-α-induced NF-κB ***,expression of proinflammatory cytokines is suppressed by ***14 inhibits the phosphorylation of IKKs by interacting with IKKs and ***,nsp14 suppresses TNF-α-induced phosphorylation and nuclear import of *** nsp14 considerably increases PEDV *** results suggest a novel mechanism employed by PEDV to suppress the host antiviral response,providing insights that can guide the development of antivirals against CoVs.

关键词： CoVs PEDV nsp14 NF-κB innate immunity

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A novel dual-functional MEMS sensor integrating both pressure and temperature units

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Journal of Semiconductors 2010年第7期31卷 65-71页

作者：陈涛张兆华任天令缪顾进周长见林惠旺刘理天 national laboratory for Information Science and Technology Institute of MicroelectronicsTsinghua University

This paper proposes a novel miniature dual-functional sensor integrating both pressure and temperature sensitive units on a single *** device wafer of SOI is used as a pizeoresistive diaphragm which features excellent consistency in *** conventional anisotropic wet etching has been abandoned,while ICP etching has been employed to etch out the reference cave to minimize the area of individual device in the way that the 57.4°slope has been *** a result,the average cost of the single chip is *** PN junctions with constant ratio of the areas of depletion regions have also been integrated on the same chip to serve as a temperature sensor,and each PN junction shows high linearity over -40 to 100℃and low power *** iron implanting process for PN junction is exactly compatible with the piezoresistor,with no additional *** pressure sensitivity is 86 mV/MPa,while temperature sensitivity is 1.43 mV/℃,both complying with the design objective.

关键词： SOI MEMS pressure sensor temperature sensor

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Spatial quantum coherent modulation with perfect hybrid vector vortex beam based on atomic medium

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Chinese Physics B 2024年第2期33卷 360-364页

作者：马燕杨欣常虹杨鑫琪曹明涛张晓斐高宏董瑞芳张首刚 Key laboratory of Time reference and Applications National Time Service CenterChinese Academy of SciencesXi’an 710600China School of Astronomy and Space Science University of Chinese Academy of SciencesBeijing 100049China Ministry of Education Key laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter Shaanxi Key Laboratory of Quantum Information and Quantum Optoelectronic DevicesSchool of ScienceXi’an Jiaotong UniversityXi’an 710049China Department of Physics Shaanxi University of Science and TechnologyXi’an 710021China Hefei national laboratory Hefei 230088China

The perfect hybrid vector vortex beam(PHVVB)with helical phase wavefront structure has aroused significant concern in recent years,as its beam waist does not expand with the topological charge(TC).In this work,we investigate the spatial quantum coherent modulation effect with PHVVB based on the atomic medium,and we observe the absorption characteristic of the PHVVB with different TCs under variant magnetic *** find that the transmission spectrum linewidth of PHVVB can be effectively maintained regardless of the ***,the width of transmission peaks increases slightly as the beam size expands in hot atomic *** distinctive quantum coherence phenomenon,demonstrated by the interaction of an atomic medium with a hybrid vector-structured beam,might be anticipated to open up new opportunities for quantum coherence modulation and accurate magnetic field measurement.

关键词： perfect hybrid vector vortex beam topological charge quantum coherence optical manipulation

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Linguistic Theory Based Contextual Evidence Mining for Statistical Chinese Co-reference Resolution

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Journal of Computer Science & Technology 2007年第4期22卷 608-617页

作者：赵军刘非凡 national laboratory of Pattern Recognition Institute of AutomationChinese Academy of Sciences

Under statistical learning framework, the paper focuses on how to use traditional linguistic findings on anaphora resolution as a guide for mining and organizing contextual features for Chinese co-reference resolution. The main achievements are as follows. （1） In order to simulate ＂syntactic and semantic parallelism factor＂, we extract ＂bags of word form and POS＂ feature and ＂bag of seines＂ feature from the contexts of the entity mentions and incorporate them into the baseline feature set. （2） Because it is too coarse to use the feature of bags of word form, POS tag and seme to determine the syntactic and semantic parallelism between two entity mentions, we propose a method for contextual feature reconstruction based on semantic similarity computation, in order that the reconstructed contextual features could better approximate the anaphora resolution factor of ＂Syntactic and Semantic Parallelism Preferences＂. （3） We use an entity-mention-based contextual feature representation instead of isolated word-based contextual feature representation, and expand the size of the contextual windows in addition, in order to approximately simulate ＂the selectional restriction factor＂ for anaphora resolution. The experiments show that the multi-level contextual features are useful for co-reference resolution, and the statistical system incorporated with these features performs well on the standard ACE datasets.

关键词： natural language processing information extraction co-reference resolution anaphora resolution

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Coherent optical frequency transfer via 972-km fiber link

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Chinese Physics B 2024年第2期33卷 277-282页

作者：邓雪张翔臧琦焦东东王丹刘杰高静许冠军董瑞芳刘涛张首刚 national Time Service Centre Chinese Academy of SciencesXi’an 710600China Key laboratory of Time reference and Application Chinese Academy of SciencesXi’an 710600China School of Communications and Information Engineering and School of Artificial Intelligence Xi’an University of Posts and TelecommunicationsXi’an 710121Chin

We demonstrate coherent optical frequency dissemination over a distance of 972 km by cascading two spans where the phase noise is passively compensated *** of employing a phase discriminator and a phase locking loop in the conventional active phase control scheme,the passive phase noise cancellation is realized by feeding double-trip beat-note frequency to the driver of the acoustic optical modulator at the local *** passive scheme exhibits fine robustness and reliability,making it suitable for long-distance and noisy fiber *** optical regeneration station is used in the link for signal amplification and cascaded *** phase noise cancellation and transfer instability of the 972-km link is investigated,and transfer instability of 1.1×10^(-19)at 10^(4)s is *** work provides a promising method for realizing optical frequency distribution over thousands of kilometers by using fiber links.

关键词： optical frequency transfer fiber link phase noise cancellation

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Development of High Performance Liquid Chromatography-Tandem Mass Spectrometry Method for the Detection of Tulathromycin in Swine Plasma

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Journal of Integrative Agriculture 2012年第3期11卷 465-473页

作者： HUANG Xian-hui ZHAO Yong-da HE Li-min LIANG Zi-sen GUO Li-li ZENG Zhen-ling CHEN ZHANG-liu ZHANG Min FANG Bing-hu national reference laboratory of Veterinary Drug Residues College of Veterinary Medicine South China Agricultural UniversityGuangzhou 510642 P.R. China Qingdao Conyinent Phaimaceutical Co. Ltd. Qingdao 266061 P.R. China

An accurate and precise method for the determination of tulathromycin in swine plasma was developed and *** samples were analyzed by high-performance liquid chromatography with tandem mass spectrometry detection （HPLC-MS/MS） using electrospray ionization （ESI）.Tulathromycin was extracted from plasma by precipitation with acetonitrile and separated using a Phenomenex Luna 5 μm C18 column （150 mm×2.0 mm） at a flow rate of 0.25 mL min^-*** A consisted of 0.002 mol L^-1 ammonium acetate and formic acid （999：1,v/v）,and solvent B was *** mass spectrometer was operated in the selected-ion mode with atmospheric pressure chemical ionization to monitor the respective MH＋ ions,namely,m/z 577.3 for tulathromycin and m/z 679.3 for the internal standard *** calibration curves were linear in a dynamic range of 2.0-500 ng mL^-1 on the *** accuracy was ranged from 95.25 to 109.75%,and the precision was ranged from 2.81 to 7.72%.The recoveries measured at 3 concentration levels （20,250,and 500 ng mL^-1） were higher than 98%.The method described above is efficient,and has the required accuracy and precision for rapid determination of tulathromycin in *** method was applied to study the pharmacokinetics of tulathromycin in swine,and tulathromycin demonstrated a rapid absorption,wide distribution,and slow elimination after intramuscular administration.

关键词： tulathromycin drug assay pharmacokinetics plasma swine HPLC-MS/MS

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New Simian Enterovirus 19(EV-A122) Strains in China Reveal Large-Scale Inter-Serotype Recombination between Simian EV-As

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Virologica Sinica 2021年第6期36卷 1652-1655页

作者： Zhenzhi Han Jinbo Xiao Yang Song Shuangli Zhu Dongyan Wang Huanhuan Lu Tianjiao Ji Dongmei Yan Wenbo Xu Yong Zhang WHO WPRO Regional Polio reference laboratory NHC Key Laboratory for BiosafetyNHC Key Laboratory for Medical VirologyNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing102206China Center for Biosafety Mega-Science Chinese Academy of SciencesWuhan430071China

Dear Editor,The genus Enterovirus(EV),belonging to the family Picornaviridae,order Picornavirales,comprises 15 species:Enterovirus A–L and rhinovirus A–C(Zell et al.2017).Enterovirus A(EV-A)has 25 serotypes,including several pathogens,such as EV-A71,coxsackievirus A16(CV-A16),and CV-A6 that infect humans,as well as four serotypes(EV-A122,123,124,and 125)isolated from simians(Zell et al.2017).EV genomes share a similar structure with a length of 7.5 kb,and two open reading frames(ORFs)flanked by 5′-and 3′-untranslated regions(UTRs)in some EVs(Oberste et al.2013b;Zell et al.2017;Guo et al.2019;Lulla et al.2019).

关键词： Enterovirus frames

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Core and variable antimicrobial resistance genes in the gut microbiomes of Chinese and European pigs

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Zoological Research 2024年第1期45卷 189-200页

作者： Cui-Hong Tong Zhi-Peng Huo Lu Diao Dan-Yu Xiao Ruo-Nan Zhao Zhen-Ling Zeng Wen-Guang Xiong Guangdong Provincial Key laboratory of Veterinary Pharmaceutics Development and Safety Evaluation South China Agricultural UniversityGuangzhouGuangdong 510642China national Risk Assessment laboratory for Antimicrobial Resistance of Animal Original Bacteria College of Veterinary MedicineSouth China Agricultural UniversityGuangzhouGuangdong 510642China national laboratory of Safety Evaluation(Environmental Assessment)of Veterinary Drugs South China Agricultural UniversityGuangzhouGuangdong 510642China national reference laboratory of Veterinary Drug Residues South China Agricultural UniversityGuangzhouGuangdong 510642China

Monitoring the prevalence of antimicrobial resistance genes(ARGs)is vital for addressing the global crisis of antibiotic-resistant bacterial *** its importance,the characterization of ARGs and microbiome structures,as well as the identification of indicators for routine ARG monitoring in pig farms,are still lacking,particularly concerning variations in antimicrobial exposure in different countries or ***,metagenomics and random forest machine learning were used to elucidate the ARG profiles,microbiome structures,and ARG contamination indicators in pig manure under different antimicrobial pressures between China and *** showed that Chinese pigs exposed to high-level antimicrobials exhibited higher total and plasmid-mediated ARG abundances compared to those in European pigs(P<0.05).ANT(6)-Ib,APH(3')-IIIa,and tet(40)were identified as shared core ARGs between the two pig ***,the core ARGs identified in pig populations were correlated with those found in human populations within the same geographical *** and Prevotella were identified as the dominant genera in the core microbiomes of Chinese and European pigs,*** ARG markers and 43 biomarkers were able to differentiate between the Chinese and European pig manure samples with accuracies of 100%and 98.7%,*** for assessing ARG contamination in Chinese and European pigs also achieved high accuracy(r=0.72-0.88).Escherichia flexneri in both Chinese and European pig populations carried between 21 and 37 *** results of this study emphasize the importance of global collaboration in reducing antimicrobial resistance risk and provide validated indicators for evaluating the risk of ARG contamination in pig farms.

关键词： Metagenomic Pig manure Antimicrobial pressure Antimicrobial resistance genes Microbiome

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