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Exploration of an Efficient Simultaneous Molecular Detection Method of hiv,HCV,and Syphilis from a Single Dried Blood Spot

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Biomedical and Environmental Sciences 2021年第4期34卷 257-264页

作者： MA Jie Qiong XU Qing Qing HE Lin HE Xiao Xia CHEN Kai WANG Yue Hua XING Wen Ge JIANG Yan national hiv reference laboratory National Center for AIDS/STD Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China

Objective The aim of the present study was to evaluate the performance of the simultaneous detection of hiv-1 RNA,hiv-1 DNA,and HCV RNA using one dried blood spot(DBS)as an alternative sample to *** A total of 571 paired DBS/plasma samples were collected from men who have sex with men(MSM)and injection drug users(IDUs),and serological and molecular assays were *** plasma results as the reference standard,the performance of DBS tests for hiv-1 RNA,hiv-1 DNA,and HCV RNA was ***’s correlation coefficients and Bland-Altman analysis were performed to assess the correlation and concordance between DBS and *** Among paired plasma/DBS samples with detectable hiv-1 RNA and HCV RNA,five samples(5/32)were not detectable in DBS,while measurable hiv-1 RNA levels were present in plasma(1.44 to3.99 log10 copies/m L).There were two samples(2/94)with undetectable HCV RNA in DBS,while measurable HCV RNA levels were present in plasma(-5 to 5.99 log10 copies/m L).The correlation between hiv-1 RNA light chain variable region(VL)values obtained from plasma and DBS showed that r=0.683(Phiv-1 RNA,the mean(±SD)difference between hiv-1 RNA in plasma and DBS was 1.00±1.01 log10 copies/m L,and all samples were within±1.96 SD(-0.97 to 2.97 log10 copies/m L)for *** mean difference(±SD)in HCV RNA was 0.15±1.08 log10 copies/m L,and 94.38%(84/89)were within±1.96 SD(-1.96 to 2.67 log10 copies/m L).Overall,hiv-1 RNA and HCV RNA levels obtained from a DBS were lower than those obtained from ***-1 DNA in a DBS showed concordant results with hiv-1 RNA in ***-1 DNA RT-PCR using a DBS showed acceptable *** The performance of the simultaneous detection of hiv-1 RNA,hiv-1 DNA,and HCV RNA using one DBS was ***,as an alternative sample to plasma,may be a viable option for the simultaneous detection of hiv-1 RNA,hiv-1 DNA,and HCV R

关键词： Dried blood spot(DBS) Correlation Bland-Altman hiv-1 RNA hiv-1 DNA HCV RNA

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Estimating hiv Incidence Rates among MSM in an Urban Area of Chongqing Using Three Approaches

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Biomedical and Environmental Sciences 2017年第12期30卷 913-916页

作者： HAN Mei PAN Ping Liang WU Guo Hui ZHOU Chao YAO Yuan LUO Juan ZOU Jing Bo ZHANG Bo Department of laboratory Medicine Southwest Hospital Third Military Medical University Department of AIDS/STDs Control and Prevention Chongqing Municipal Centers for Disease Control and Prevention national reference hiv/AIDS laboratory National Center for AIDS/STDs Control and Prevention

To evaluate the hiv pandemic in Chongqing, the pooled PCR, IgG-capture BED enzyme immunoassay （BED-CEIA）, and cohort observations were used to estimate the hiv incidences among men who have sex with men （MSM）. 617 MSM subjects completed the survey at a voluntary counseling and testing （VCT） site. The observed hiv incidence was 12.5 per 100 P-Ys （95% CI = 9.1-15.7）.

关键词： Estimating hiv Urban Area

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In vitro inhibition of hiv-1 replication in autologous CD4*T cells indicates viral containment by multifactorial mechanisms

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Virologica Sinica 2017年第6期32卷 485-494页

作者： Ting Tu Jianbo Zhan Danlei Mou Wei Li Bin Su Tong Zhang Tao Li Ning Li Hao Wu Cong Jin Huabiao Chen Jiangsu Key laboratory of Clinical laboratory Medicine Jiangsu University School of MedicineZhenjiang 212013China national hiv/HCV reference laboratory National Center for AIDS/STD Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China Hubei Provincial Center for Disease Control and Prevention Wuhan 430079China Beijing You'an Hospital Capital Medical UniversityBeijing 100069China

hiv-1-specific cytotoxic T lymphocytes(CTLs) and neutralizing antibodies(NAbs) are present during chronic infection, but the relative contributions of these effector mechanisms to viral containment remain unclear. Here, using an in vitro model involving autologous CD4+ T cells,primary hiv-1 isolates, hiv-1-specific CTLs, and neutralizing monoclonal antibodies, we show that b12, a potent and broadly neutralizing monoclonal antibody to hiv-1, was able to block viral infection when preincubated with virus prior to infection, but was much less effective than CTLs at limiting virus replication when added to infected cell cultures. However, the same neutralizing antibody was able to contain viruses by antibody-dependent cell-mediated virus inhibition in vitro,which was mediated by natural killer cells(NKs) and dependent on an Fc-Fc receptor ***, bulk CTLs from hiv-1 controllers were more effective in suppression of virus replication than those from progressors. These findings indicate that control of hiv-1 replication in activated CD4^+ T cells is ineffectively mediated by neutralizing antibodies alone, but that both CTLs and antibody-dependent NK-mediated immune mechanisms contribute to viral containment. Our study systemically compared three major players in controlling hiv-1 infection, CTLs, NAbs, and NKs, in an autologous system and highlighted the multifactorial mechanisms for viral containment and vaccine success.

关键词： hiv-1 infection viral replication cytotoxic T lymphocyte(CTL) natural killer cells neutralizing antibody

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A multiplex real-time PCR assay for simultaneous detection of classical swine fever virus,African swine fever virus,and atypical porcine pestivirus

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Journal of Integrative Agriculture 2023年第2期22卷 559-567页

作者： SONG Xiang-peng XIA Ying-ju XU Lu ZHAO Jun-jie WANG Zhen ZHAO Qi-zu LIU Ye-bing ZHANG Qian-yi WANG Qin China Institute of Veterinary Drug Control/national reference laboratory for Classical Swine Fever Beijing 100081P.R.China

With the implementation of the C-strain vaccine,classical swine fever(CSF) has been under control in China,which is currently in a chronic atypical epidemic *** swine fever(ASF) emerged in China in 2018 and spread quickly across the *** is presently occurring sporadically due to the lack of commercial vaccines and farmers’ increased awareness of *** porcine pestivirus(APPV) was first detected in Guangdong Province,China,in 2016,which mainly harms piglets and has a local epidemic situation in southern *** three diseases have similar clinical symptoms in pig herds,which cause considerable losses to the pig *** are difficult to be distinguished only by clinical ***,developing an early and accurate simultaneous detection and differential diagnosis of the diseases induced by these viruses is *** this study,three pairs of specific primers and Taq-man probes were designed from highly conserved genomic regions of CSFV(5’ UTR),African swine fever virus(ASFV)(B646L),and APPV(5’ UTR),followed by the optimization of reaction conditions to establish a multiplex real-time PCR detection *** results showed that the method did not cross-react with other swine pathogens(porcine circovirus type 2(PCV2),porcine reproductive and respiratory syndrome virus(PRRSV),foot-and-mouth disease virus(FMDV),pseudorabies virus(PRV),porcine parvovirus(PPV),and bovine viral diarrhea virus BVDV).The sensitivity results showed that CSFV,ASFV,and APPV could be detected as low as 1 copy μL–1;the repeatability results showed that the intra-assay and interassay coefficient of variation of ASFV,CSFV,and APPV was less than 1%.Twenty-two virus samples were detected by the multiplex real-time PCR,compared with national standard diagnostic and patented method assay for CSF(GB/T 27540–2011),ASF(GB/T 18648–2020),and APPV(CN108611442A),*** sensitivity of this triple real-time PCR for CSFV,ASFV,and APPV was almost the

关键词： classical swine fever virus African swine fever virus atypical porcine pestivirus real-time PCR

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A Seroprevalence Survey of Maedi-Visna Among Twenty-Four Ovine Floks from Twelve Regions of China

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Journal of Integrative Agriculture 2013年第12期12卷 2321-2323页

作者： ZHANG Ke-shan HE Ji-jun LIU Yong-jie SHANG You-jun LIU Xiang-tao State Key laboratory of Veterinary Etiological Biology/national Foot and Mouth Disease reference laboratory Lanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences

Maedi-visna virus（MVV） is an ovine lentivirus that is widespread in many countries *** clinical and subclinical MVV infections cause substantial economic *** infection in live sheep is usually diagnosed serologically,with antibody-positive sheep being regarded as *** have been few reports of maedi-visna in China,with no detailed epidemic analysis of MVV infection in ovine *** order to investigate the seroprevalence and spatial distribution of maedi-visna among ovine flocks in China,a total of 672 serum samples were collected from different ovine flocks in 12 regions（provinces,autonomous regions or municipalities） of China in 2011,and serum antibody levels were determined using a commercial ELISA *** study represents the first investigation of the seroepidemiology of maedi-visna in China,indicating a circulation of MMV among sheep.

关键词： seroprevalence spatial distribution maedi-visna

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Influence of PPV,PRV and PRRSV on Efficacy of the Lapinized Hog Cholera Vaccine and Pathogenicity of Classical swine fever virus

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Journal of Integrative Agriculture 2012年第11期11卷 1892-1897页

作者： NING Yi-bao ZHAO Yun WANG Qin FAN Xue-zheng QIN Yu-ming ZHANG Guang-chuan XU Lu QIU Hui-shen WANG Zai-shi SONG Li SHEN Qing-chun ZHAO Qi-zu China national Classical Swine Fever reference laboratory/China Institute of Veterinary Drug Control Beijing 100081 P.R. China

Classical swine fever caused by Classical swine fever virus （CSFV） is a serious problem for swine industries in developing countries, which successful control of the disease have been relying on vaccination. However, classical swine fever still occurs in some immunized swine herds for various reasons. In this study, we conducted animal experiments to examine the influence of single or mixed infection with Porcine parvo virus （PPV）, Pseudorabies virus （PRV） and Porcine reproductive and respiratory syndrome virus （PRRSV） on the protective immunity induced by the Lapinized hog cholera virus （HCLV） vaccine and the pathogenicity of CSFV. In experiment 1, pigs were first inoculated with PPV, PRV or PRRSV, then immunized with HCLV, and finally challenged with a highly virulent CSFV Shimen strain. All of the pigs immunized with HCLV survived after the challenge, while all of the pigs in the non-immunized control group died after the challenge. The pigs in the group immunized with HCLV did not show any clinical symptoms of classical swine fever and were negative with CSFV after the challenge. The pigs infected with the non-CSFV before HCLV immunization did not display any clinical symptoms after the challenge with CSFV Shiman strain, but 11 of the 12 pigs were positive with CSFV. In experiment 2, pre-infections with PPV, PRV, and PRRSV were followed by inoculation with a low-virulence CSFV strain （CSFV 39）, and then the pigs were challenged with the CSFV Shimen strain. Infections by either PPV, PRV or PRRSV did not enhance the virulence of CSFV-39, but pigs infected by a mixture of the 3 viruses developed clinical symptoms after inoculation with CSFV-39. The mixed infection also increased mortality caused by the challenge with the CSFV Shimen strain. Together, these results showed PPV, PRV and PRRSV infections in pigs can reduce the efficacy of the HCLV vaccine and enhance the pathogenicity of CSFV, which may partly explain the immunization failure against CSFV in some swine

关键词： Lapinized hog cholera virus vaccination pathogenicity mixed infection influence

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Screening and Identification of Marburg Virus Entry Inhibitors Using Approved Drugs

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Virologica Sinica 2020年第2期35卷 235-239页

作者： Li Zhang Shan Lei Hui Xie Qianqian Li Shuo Liu Qiang Liu Weijin Huang Xinyue Xiao Youchun Wang Division of hiv/AIDS and Sexually-transmitted Virus Vaccines National Institutes for Food and Drug ControlBeijing 102629China Institute for reference Standards and Standardization National Institutes for Food and Drug ControlBeijing 102629China

Dear Editor,Marburg virus(MARV)belongs to the Filoviridae family,along with the related Ebola virus(EBOV).Although MARV is less renowned than EBOV,it causes an equally devastating disease,with clinical symptoms similar to EBOV infection and a remarkably high mortality *** date,about 600 MARV cases have been reported globally,with an average mortality rate of around 80%(Brauburger et al.2012).Due to the severity of disease caused by MARV,as well as its threat to global public health,MARV is classified as a risk group 4 pathogen by WHO,necessitating containment measures equivalent to biosafety level 4(BSL-4).

关键词： mortality al. clinical

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Assessing healthcare workers’knowledge and confidence in the diagnosis,management and prevention of Monkeypox

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World Journal of Clinical Cases 2025年第1期13卷 38-47页

作者： Epipode Ntawuyamara Thierry Ingabire Innocent Yandemye Polycarpe Ndayikeza Bina Bhandari Yan-Hua Liang Department of Dermatology Cosmetology and VenereologyShenzhen Hospital of Southern Medical UniversityShenzhen 518101Guangdong ProvinceChina Department of Dermatology and Venereology Kamenge Teaching HospitalUniversity of BurundiBujumbura 1020Burundi Department of Infectious Diseases Kamenge Teaching HospitalUniversity of BurundiBujumbura 1020Burundi General Directorate of Healthcare Supply Modern and Traditional MedicineNutrition and RegistrationMinistry of Public Health and Fight Against HIV/AIDSBujumbura 1055Burundi Department of Epidemiology National Institute of Public HealthBujumbura 350Burundi General Directorate of Planification Ministry of Public Health and Fight Against HIV/AIDSBujumbura 1055Burundi

BACKGROUND Monkeypox(Mpox),is a disease of global public health concern,as it does not affect only countries in western and central *** To assess Burundi healthcare workers(HCWs)s’level of knowledge and confidence in the diagnosis and management of *** We conducted a cross-sectional study via an online survey designed mainly from the World Health Organization course distributed among Burundi HCWs from June-July *** questionnaire comprises 8 socioprofessional-related questions,22 questions about Mpox disease knowledge,and 3 questions to assess confidence in Mpox diagnosis and *** data were analyzed via SPSS software version 25.0.A P value<0.05 was considered to indicate statistical *** The study sample comprised 471 HCWs who were mainly medical doctors(63.9%)and nurses(30.1%).None of the 22 questions concerning Mpox knowledge had at least 50%correct responses.A very low number of HCWs(17.4%)knew that Mpox has a *** confidence level to diagnose(21.20%),treat(18.00%)or prevent(23.30%)Mpox was low among *** confidence level in the diagnosis of Mpox was associated with the HCWs’age(P value=0.009),sex(P value<0.001),work experience(P value=0.002),and residence(P value<0.001).The confidence level to treat Mpox was significantly associated with the HCWs’age(P value=0.050),sex(P value<0.001),education(P value=0.033)and occupation(P value=0.005).The confidence level to prevent Mpox was associated with the HCWs’education(P value<0.001),work experience(P value=0.002),residence(P value<0.001)and type of work institution(P value=0.003).CONCLUSION This study revealed that HCWs have the lowest level of knowledge regarding Mpox and a lack of confidence in the ability to diagnose,treat or prevent *** is an urgent need to organize continuing medical education programs on Mpox epidemiology and preparedness for Burundi *** encourage future researchers to assess potential hesitancy toward Mpox vaccination and its asso

关键词： Monkeypox Public health emergency of international concern Healthcare workers Epidemic Preparedness Knowledge Confidence

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Comparative study on anti-HCV testing using plasma, dried plasma spots (DPS), and dried blood spots (DBS)

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Biosafety and Health 2021年第1期3卷 50-55页

作者： Jieqiong Ma Lin He Kai Chen Yuehua Wang Yanan Ren Wenge Xing Yan Jiang national hiv/HCV reference laboratory National Center for AIDS/STD Control and PreventionChina CDCBeijing 102206China

The aim of this study was to evaluate the performance of an assay using dried plasma spot(DPS)and dried blood spot(DBS)samples for the serological detection of anti-hepatitis C virus(HCV)*** January and July 2019,plasma,DPS and DBS specimens were collected from individuals at high-risk for HCV *** were tested for anti-HCV by ELISA,and the performance of DPS and DBS specimens was examined using results from the plasma testing,as the *** samples were collected from 329 persons,including 129 men who have sex with men and 200 intravenous drug *** from the plasma testing indicated that 118 samples(59.0%)were HCV *** from the DPS sample testing showed sensitivity as 99.2%(95%confidence interval[CI]:0.95-1.00)and specificity as 100%(95%CI:0.98-1.00)for HCV detection,with Kappa of 99.3%(95%CI:0.98-1.00)while in DBS sample testing the sensitivity as 98.3%(95%CI:0.93-1.00)and specificity as 100%(95%CI:0.98-1.00),with Kappa of 98.7%(95%CI:0.97-1.00),***’s correlation coefficients for the comparisons between plasma and DPS specimen,plasma and DBS specimens,DPS and DBS specimens were 0.857,0.750,and 0.739,*** with the results in plasma,1 sample was not detected using the DPS specimens,and 2 samples were failed for the positive detection,using the DBS *** DPS and DBS samples were promising alternatives to plasma,for the detection of anti-HCV antibodies.

关键词： HCV Plasma Dried plasma spots Dried blood spots Spearman's correlation coefficient

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African Swine Fever Virus MGF-110-9L-deficient Mutant Has Attenuated Virulence in Pigs

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Virologica Sinica 2021年第2期36卷 187-195页

作者： Dan Li Yinguang Liu Xiaolan Qi Yuan Wen Pan Li Zhao Ma Yongjie Liu Haixue Zheng Zhijie Liu State Key laboratory of Veterinary Etiological Biology and OIE/national Foot and Mouth Disease reference laboratory Lanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou 730046China

African swine fever virus(ASFV)is the etiological agent of African swine fever(ASF),an often lethal disease in domestic and wild *** represents a major threat to the swine industry ***,no commercial vaccine is available because of the complexity of ASFV or biosecurity *** attenuated viruses that are naturally isolated or genetically manipulated have demonstrated reliable protection against homologous ASFV strain *** the present study,a mutant ASFV strain with the deletion of ASFV MGF-110-9L(ASFV-D9L)was generated from a highly virulent ASFV CN/GS/2018 parental strain,a genotypeⅡ*** to the parental ASFV isolate,deletion of the MGF-110-9L gene significantly decreased the ability of ASFV-D9L to replicate in vitro in primary swine macrophage cell *** majority of animals inoculated intramuscularly with a low dose of ASFV-D9L(10 HAD50)remained clinically normal during the 21-day observational *** of five ASFV-D9L-infected animals displayed low viremia titers and low virus shedding and developed a strong virus-specific antibody response,indicating partial attenuation of the ASFV-D9L strain in *** findings imply the potential usefulness of the ASFV-D9L strain for further development of ASF control measures.

关键词： African swine fever virus(ASFV) MGF-110-9L Mutant Attenuated virulence Pig

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