检索结果-南通市图书馆

High-Level Accumulation of Exogenous Small RNAs Not Affecting Endogenous Small RNA Biogenesis and Function in Plants

引用

Journal of Integrative Agriculture 2014年第5期13卷 1017-1023页

作者： SHEN Wan-xia Neil A Smith ZHOU Chang-yong WANG Ming-bo College of Plant Protection Southwest University CSIRO Plant Industry national Citrus engineering research center Citrus Research Institute Chinese Academy of Agricultural Sciences

RNA silencing is a fundamental plant defence and gene control mechanism in plants that are directed by 20-24 nucleotide （nt） small interfering RNA （siRNA） and microRNA （miRNA）. Infection of plants with viral pathogens or transformation of plants with RNA interference （RNAi） constructs is usually associated with high levels of exogenous siRNAs, but it is unclear if these siRNAs interfere with endogenous small RNA pathways and hence affect plant development. Here we provide evidence that viral satellite RNA （satRNA） infection does not affect siRNA and miRNA biogenesis or plant growth despite the extremely high level of satRNA-derived siRNAs. We generated transgenic Nicotiana benthamiana plants that no longer develop the specific yellowing symptoms generally associated with infection by Cucumber mosaic virus （CMV） Y-satellite RNA （Y-Sat）. We then used these plants to show that CMV Y-Sat infection did not cause any visible phenotypic changes in comparison to uninfected plants, despite the presence of high-level Y-Sat siRNAs. Furthermore, we showed that the accumulation of hairpin RNA （hpRNA）-derived siRNAs or miRNAs, and the level of siRNA-directed transgene silencing, are not significantly affected by CMV Y-Sat infection. Taken together, our results suggest that the high levels of exogenous siRNAs associated with viral infection or RNAi-inducing transgenes do not saturate the endogenous RNA silencing machineries and have no significant impact on normal plant development.

关键词： Nicotiana benthamiana transformation satellite RNA RNA silencing small RNA

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The potential of ocean acidification on suppressing larval development in the Pacific oyster Crassostrea gigas and blood cockle Area inflata Reeve

引用

Chinese Journal of Oceanology and Limnology 2014年第6期32卷 1307-1313页

作者：李加琦蒋增杰张继红毛玉泽卞大鹏方建光 Mariculture Ecology and Carrying Capacity Laboratory Yellow Sea Fisheries Research InstituteChinese Academy of Fisheries Sciences national engineering research center of Marine Shellfish

We evaluated the effect of pH on larval development in larval Pacific oyster(Crassostrea gigas) and blood cockle(Arca inflata Reeve).The larvae were reared at pH 8.2(control),7.9,7.6,or 7.3beginning 30 min or 24 h post *** to lower pH during early embryonic development inhibited larval shell formation in both *** with the control,larvae took longer to reach the D-veliger stage when reared under pH 7.6 and *** to lower pH immediately after fertilization resulted in significantly delayed shell formation in the Pacific oyster larvae at pH 7.3 and blood cockle larvae at pH 7.6 and ***,when exposure was delayed until 24 h post fertilization,shell formation was only inhibited in blood cockle larvae reared at pH ***,the early embryonic stages were more sensitive to acidified *** results suggest that ocean acidification will have an adverse effect on embryonic development in *** the effects appear subtle,they may accumulate and lead to subsequent issues during later larval development.

关键词： ocean acidification larval development bivalve calcifier

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

RNA-Seq transcriptome analysis of porcine cloned and in vitro fertilized blastocysts

引用

Journal of Integrative Agriculture 2015年第5期14卷 926-938页

作者： XU Wei-hua LI Zi-cong OUYANG Zhi-ping YU Bo SHI Jun-song LIU De-wu WU Zhen-fang national engineering research center for Breeding Swine Industry South China Agricultural University College of Life Science Longyan University Wen's research Institute

Somatic nuclear transfer technology has become increasingly promising in biomedicine and *** the approach remains inefficient and underlying mechanisms remain *** cloned embryos have similar in vitro developmental capacity as in vitro fertilized（IVF） embryos before implantation,they appeared to have much lower full-term developmental efficiency in pig and cattle,and thus it would be reasonable to postulate that profound distinction at the molecular level should exist between ***,RNA sequencing technique was used to screen differentially expressed genes in cloned and IVF blastocysts,and in total 628 differentially expressed transcripts were obtained,among which,280 transcripts are up-regulated and 348 transcripts are down-regulated in cloned ***,one statistically significant pathway associated with endoplasmic reticulum（ER） protein processing was enriched,and some ER-stress markers such as ATF4,ATF6,PDIA3,HSPA1 B,HSP40 and HSP90 between cloned and IVF blastocysts were ***,some developmentally important genes such as lipid metabolism related genes（MGLL,DDHD2 and FADS2） and epigenetic modification genes（DNMT1,KDM5 C and MBD3L5） were found differentially expressed between cloned and IVF embryos.

关键词： cloned transcripts RNA porcine developmental endoplasmic reticulum differentially cloning enriched

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Thrust control of superconducting DC linear motor for electromagnetic launch

Thrust control of superconducting DC linear motor for electr...

引用

第36届中国控制会议

作者： Guanchun Li Jie Li engineering research center of Maglev Technology National University of Defense Technology

This paper presents a thrust control based on current *** controller is designed based on the relationship between current and back-EMF,and the control scheme is assessed through simulation *** results demonstrate cur... 详细信息

ISBN: (纸本)9781538629185

关键词： Thrust control current compensation SDCLM

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

A lateral-immobilization zebrafish microfluidic chip-based system for in vivo real-time evaluation of antithrombotic agents

引用

Chinese Chemical Letters 2024年第3期35卷 312-315页

作者： Lijuan He Hongxia Du Yi Yang Zhihua Guan Jinjin Li Honglin Li Xudong Lin Lili Zhu Shanghai Key Laboratory of New Drug Design School of PharmacyEast China University of Science&TechnologyShanghai 200237China Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument School of Biomedical EngineeringShenzhen Campus of Sun Yat-sen UniversityShenzhen 518000China Innovation center for AI and Drug Discovery East China Normal UniversityShanghai 200062China

Thrombosis remains a major global health concern mainly characterized by high rates of morbidity and *** models serve as an indispensable tool to understand the underlying pathogenesis of thrombosis and assess the efficacy of novel antithrombotic ***,zebrafish has emerged as a valuable model organism for thrombosis ***,the traditional method of studying zebrafish thrombosis requires a laborious and time-consuming procedure,including anesthesia and manual immobilization of *** this study,based on hydrodynamic force,a lateral-immobilization zebrafish microfluidic chip(LIZMC)was designed to evaluate the cardiovascular system of multiple larvae within a single microscope field of ***,coupling with microscope imaging,real-time monitoring of the peripheral blood circulation in the tail of phenylhydrazine(PHZ)-induced zebrafish thrombosis was ***,the reliability of LIZMC for in vivo evaluation of antithrombotic agents in zebrafish was verified using ***,this novel LIZMC-based system can be used for in vivo zebrafish thrombosis studies and rapid screening of antithrombotic agents.

关键词： Microfluidic chip Zebrafish Thrombosis In vivo evaluation Real-time monitor

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Microfluidic synthesis of hollow CsPbBr_(3) perovskite nanocrystals through the nanoscale Kirkendall effect

引用

Nano research 2024年第9期17卷 8487-8494页

作者： Yue Chen Xiaoyu Zhang Jinzhou Jiang Gaoyu Chen Kunhong Zhou Xinwen Zhang Fajing Li Caojin Yuan Jianchun Bao Xiangxing Xu Jiangsu Collaborative Innovation center of Biomedical Functional Materials Jiangsu Key Laboratory of New Power BatteriesSchool of Chemistry and Materials ScienceNanjing Normal UniversityNanjing 210023China Key Laboratory for Organic Electronics and Information Displays Institute of Advanced Materials(IAM)Nanjing University of Posts and TelecommunicationsNanjing 210023China Frontiers Science center for Flexible Electronics Institute of Flexible Electronics(IFE)Northwestern Polytechnical UniversityXi’an 710072China Jiangsu Key Laboratory for Opto-Electronic Technology School of Physics and TechnologyNanjing Normal UniversityNanjing 210023China State Key Laboratory of Coordination Chemistry School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210093China

All inorganic metal halide perovskite nanocrystals(NCs)have attracted much attention for their outstanding optoelectronic properties,which can be tuned by the composition,surface,size and morphology in ***,we report the microfluidic synthesis of hollow CsPbBr_(3)perovskite NCs through the nanoscale Kirkendall *** formation mechanism of the hollow structure(Kirkendall void)controlled by the temperature,flow rate,ratios of precursors and ligands was *** with the solid CsPbBr_(3)NCs of the same size,the hollow CsPbBr_(3)NCs exhibit blue shifts in ultraviolet-visible(UV-vis)absorption and photoluminescence(PL)spectra,and remarkably longer PL average lifetime(~98.2 ns).Quantum confinement effect,inner surface induced additional trap states and lattice strain of the hollow CsPbBr_(3)NCs were discussed in understanding their unique optoelectronic *** hollow CsPbBr_(3)NC based photodetector exhibits an outstanding negative photoconductivity(NPC)detectivity of 8.9×10^(12)*** also show potentials in perovskite NC based photovoltaic and light emitting diodes(LEDs).

关键词： perovskite nanocrystals hollow nanocrystals nanoscale Kirkendall effect microfluidic synthesis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synthesis, Structure and Magnetic Property of a Binuclear Nickel(Ⅱ) Complex

引用

Chinese Journal of Structural Chemistry 2010年第4期29卷 582-586页

作者：何敏李庆丰谢婷徐国敏于杰李伟 Materials and Metallurgy College Guizhou University Guiyang national engineering research center for Compounding and Modification of Polymeric Materials Guiyang

A new binuclear complex [Ni2（2,2＇-bpy）2（C8H3NO6）2（H2O）4] 1 （2,2＇-bpy = 2,2＇- bipyridine, C8H3NO6 = 4-nitrophthalate） has been synthesized by a hydrothermal reaction and characterized by X-ray single-crystal diffraction analysis. The complex crystallizes in triclinic, space group P1 with a = 7.206（2）, b = 10.337（4）, c = 12.480（4） , α = 89.887（11）, β = 77.033（9）, γ = 78.349（6）o, V = 886.3（5） 3, Z = 1, C36H30Ni2N6O16, Mr = 920.08, Dc = 1.724 g cm-3, F（000） = 472, μ = 1.152 mm-1, the final R = 0.0458 and wR = 0.1163 for 3372 observed reflections with I 2σ（Ⅰ）. The two Ni（Ⅱ） ions are bridged by two 4-nitrophthalates both in a bis-monodentate mode. Magnetic measurements reveal that the intramolecular exchange couplings in the dimer are ferromagnetic with 2J/k = 2.32 K, D =-1.44 K and g = 2.18.

关键词： crystal structure Ni(Ⅱ) complex 4-nitrophthalate ferromagnetic interactions

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Global assessment of terrestrial productivity in response to water stress

引用

Science Bulletin 2024年第15期69卷 2352-2356页

作者： Qi Liu Huadong Guo Jiahua Zhang Shijie Li Ji Li Fengmei Yao Miguel D.Mahecha Jian Peng School of Computer engineering Jiangsu University of TechnologyChangzhou 213001China College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijing 101408China Department of Remote Sensing Helmholtz Centre for Environmental ResearchUFZLeipzig 04318Germany Remote Sensing Centre for Earth System research Leipzig UniversityLeipzig 04103Germany Aerospace Information research Institute Chinese Academy of SciencesBeijing 100094China Remote Sensing and Digital Earth center School of Computer Science and TechnologyQingdao UniversityQingdao 266071China Key Laboratory of Earth Observation of Hainan Province Hainan Aerospace Information Research InstituteChinese Academy of SciencesSanya 572000China School of Geographical Sciences Nanjing University of Information Science and TechnologyNanjing 210044China Key Laboratory of Water Cycle and Related Land Surface Processes Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China German Centre for Integrative Biodiversity research(iDiv) Halle-Jena-Leipzig 04103Germany

In recent decades, climate warming has led to an increase in atmospheric water demand, a potential reduction in soil moisture and a rise in the frequency and intensity of droughts. This has intensified the effects of water stress on plants, leading to more vegetated areas experiencing water stress [1,2]. Soil moisture (SM)and vapor pressure deficit (VPD) are considered the two main factors that exert water stress on vegetation activity and terrestrial productivity, thereby affecting the global carbon cycle [3].

关键词： soil moisture terrestrial

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of Rolling on a Warm Compacted Ti_3SiC_2 Particulate Reinforced Cu Matrix Composite

引用

稀有金属材料与工程 2011年第S3期40卷 56-58页

作者： Ngai Tungwai national engineering research center of Near-net-shape Forming Technology for Metallic Materials South China University of Technology

Warm compaction was employed to fabricate a Ti3SiC2 particulate reinforced copper matrix composite for electro-friction application. Copper matrix composite reinforced with 5wt% of copper-coated Ti3SiC2 particulates were prepared by compacting mixed powder with a pressure of 700 MPa at 145 ℃, and then sintered at 1000 ℃ under cracked ammonia atmosphere for 60 min. In order to improve the density, rolling process was applied on the sintered samples, their density, hardness, electrical conductivity, ultimate tensile strength and tribological behaviors were studied. Results showed that the rolled composite with 30% deformation has a density of 8.28 g/cm3, a hardness of 1060 MPa (HB), an ultimate tensile strength of 288 MPa, an electrical resistivity of 7.0 ×10-8 Ω·m and a friction coefficient of 0.17.

关键词： Cu-Ti3SiC2 composite powder metallurgy electro-friction material

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Identifying superword level parallelism with extended directed dependence graph reachability

引用

Science China(Information Sciences) 2017年第1期60卷 214-216页

作者： Jie ZHAO Rongcai ZHAO national Digital Switching System engineering & Technological research center Computer Science Department cole Normale Supérieure

Dear editor,The increasing trend of multimedia applications’proliferation brings about multimedia extensions’wide use among existing microprocessors of mainstream manufacturers,e.g.,Intel’s MMX,AMD’s3DNow!,IBM’s VMX/Altivec,***,the implementation details behind these multimedia extensions vary from one architecture to another;hence,it generally comes in form of SIMD(single

关键词： node SIMD Identifying superword level parallelism with extended directed dependence graph reachability SLP

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：