检索结果-南通市图书馆

Journal of Integrative Plant Biology 2008年第4期50卷 484-490页

作者： Tayyaba Sultana Abdul Ghafoor Government College University Faisalabad PakistanPlant Genetic Resources Program Institute of Agri Biotechnology and Genetic Resources National Agricultural Research Centre Islamabad Pakistan Government College University Faisalabad Pakistan

Lentil, one of the oldest legumes was investigated for diversity based on botanical descriptors, total seed proteins, isozymes and random amplified polymorphic DNA （RAPD） markers. About one fourth of accessions were heterogeneous for botanical descriptors and a seed protein profile. The germplasm collected from the province of Baluchiatan revealed the prevalence of indigenous landraces as high diversity was observed for all of the techniques. Diversity explored through various techniques revealed validity irrespective of the sample size or geographic pattem, RAPD being the best choice for investigating both inter- and intra-acceaaion variation in lentil. Although all of the techniques were able to resolve genetic diversity in lentil, isozymea and seed proteins gave low levels of genetic diversity, suggesting that more investigation into isozymea of specific proteins is required. RAPD is the best option for determining inter- and intra-acceaaion variation, and will be required to extend germplaama and primers to continue the study of botanical descriptors.

关键词： DNA marker genetic resource lentil sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2016年第30期22卷 6800-6816页

作者： Mahmoud Fathy Dondeti Eman Anwar El-Maadawy Roba Mohamed Talaat genetic Engineering and biotechnology Research Division National Research Centre Molecular Biology Department Genetic Engineering and Biotechnology Research Institute University of Sadat City

Hepatocellular carcinoma(HCC) is a primary liver cancer, which is one of the most prevalent cancers among humans. Many factors are involved in the liver carcinogenesis as lifestyle and environmental factors. Hepatitis virus infections are now recognized as the chief etiology of HCC; however, the precise mechanism is still enigmatic till now. The inflammation triggered by the cytokine-mediated immune response, was reported to be the closest factor of HCC development. Cytokines are immunoregulatory proteins produced by immune cells, functioning as orchestrators of the immune response. Genes of cytokines and their receptors are known to be polymorphic, which give rise to variations in their genes. These variations have a great impact on the expression levels of the secreted cytokines. Therefore, cytokine gene polymorphisms are involved in the molecular mechanisms of several diseases. This piece of work aims to shed much light on the role of cytokine gene polymorphisms as genetic host factor in hepatitis related HCC.

关键词： Hepatocellular carcinoma Hepatitis C virus Hepatitis B virus Cytokines Polymorphism

Functional genomics in the rice blast fungus to unravel the fungal pathogenicity

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2008年第10期9卷 747-752页

作者： Junhyun JEON Jaehyuk CHOI Jongsun PARK Yong-Hwan LEE Department of Agricultural biotechnology Center for Fungal Genetic Resources and Center for Agricultural BiomaterialsSeoul National UniversitySeoul 151-921Korea Department of Agricultural biotechnology Center for Fungal Genetic Resourcesand Center for Agricultural BiomaterialsSeoul National UniversitySeoul 151-921Korea

A rapidly growing number of successful genome sequencing projects in plant pathogenic fungi greatly increase the demands for tools and methodologies to study fungal pathogenicity at genomic scale. Magnaporthe oryzae is an economically important plant pathogenic fungus whose genome is fully sequenced. Recently we have reported the development and application of functional genomics platform technologies in M. oryzae. This model approach would have many practical ramifications in design and implementation of upcoming functional genomics studies of filamentous fungi aimed at understanding fungal pathogenicity.

关键词： Functional genomics Magnaporthe oryzae Plant pathogenic fungus Pathogenicity Rice blast

Isolation and Characterization of a C-repeat Binding Transcription Factor from Maize

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2008年第8期50卷 965-974页

作者： Lei Wang Yanzhong Luo Lan Zhang Jun Zhao Zhiqiu Hu Yunliu Fan Chunyi Zhang biotechnology Research Institute National Key Facility for Crop Gene Resources and Genetic Improvement Chinese Academy of Agricultural Sciences Beijing 100081 China

C-repeat binding proteins （CBFs） are a group of transcription factors that have been proven to be important for stress tolerance in plants. Many of these transcription factors transactivate the promoters of cold-regulated genes via binding to low temperature-or dehydration-responsive c/s-elements, thus conferring plants cold acclimation. In the present study, we isolated a C-repeat binding transcription factor from maize using the yeast one-hybrid system with the C-repeat motif from the promoter of the Arabidopsis COR15a gene as bait. The isolated transcription factor is highly similar to the Arabidopsis CBF3 in their predicted amino acid sequences, and is therefore designated ZmCBF3. Point mutation analyses of the ZmCBF3-binding c/s-element revealed （A/G）（C/T）CGAC as the core binding sequence. Expression analyses showed that ZmCBF3 was upregulated by both abscisic acid and low temperature, and was actively expressed during embryogenesis, suggesting that ZmCBF3 plays a role in stress response in maize.

关键词： C-repeat DNA-binding transcription factor Zea mays ZmCBF3

Development and drought tolerance assay of marker-free transgenic rice with OsAPX2 using biolistic particle-mediated co-transformation

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2017年第4期5卷 271-281页

作者： Dan Feng Yanwei Wang Jinxia Wu Tiegang Lu Zhiguo Zhang biotechnology Research Institute/national Key Facility for Crop Gene resources and genetic Improvement Chinese Academy of Agricultural Sciences Beijing 100081 China

Abiotic stresses such as drought, salinity, and low temperature cause–losses in rice production worldwide. The emergence of transgenic technology has enabled improvements in the drought resistance of rice plants and helped avert crop damage due to drought *** marker genes conferring resistance to antibiotics or herbicides have been widely used to identify genetically modified plants. However, the use of such markers has limited the public acceptance of genetically modified organisms. Marker-free materials (i.e., those containing a single foreign gene) may be more easily accepted by the public and more likely to find common use. In the present study, we created marker-free drought-tolerant transgenic rice plants using particle bombardment. Overall, 842 T_0plants overexpressing the rice ascorbate peroxidase-coding gene OsAPX2 were generated. Eight independentmarker-free lines were identified from T_1 seedlings using the polymerase chain *** molecular characteristics of these lines were examined, including the expression level,copy number, and flanking sequences of OsAPX2, in the T_2 progeny. A simulated drought test using polyethylene glycol and a drought-tolerance test of seedlings confirmed that the marker-free lines carrying OsAPX2 showed significantly improved drought tolerance in seedlings. In the field, the yield of the wild-type plant decreased by 60% under drought conditions compared with normal conditions. However, the transgenic line showed a yield loss of approximately 26%. The results demonstrated that marker-free transgenic lines significantly improved grain yield under drought-stressed conditions.

关键词： Rice Particle bombardment-mediated co-transformation Marker-free Drought-tolerance

Establishment of an in vitro micropropagation protocol for Boscia senegalensis (Pers.) *** Poir.

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2011年第4期12卷 303-312页

作者： Mutasim M.KHALAFALLA Hussien M.DAFFALLA Eltayb ABDELLATEF Elsadig AGABNA Hany A.EL-SHEMY Commission for biotechnology and genetic Engineering National Centre for Research Desertification Research Institute National Centre for Research Faculty of Agriculture Research Park (FARP) and Biochemistry Department Faculty of AgricultureCairo University

This report describes in vitro micropropagation of Boscia senegalensis,so-called famine foods,that helped the people in Darfur and Kordofan,Sudan survive during the 1984-1985 *** types of explants prepared from green mature zygotic embryos were cultured on Murashige and Skoog (MS) medium augmented with 1-5 mg/L 6-benzyladenine (BA).The highest number of shoots per explant (14.3±0.9) was achieved on MS medium supplemented with 3 mg/L BA,while the highest shoot length [(3.5±0.4) cm] was obtained with 1 mg/L *** shoot cluster,when subcultured to its same medium,significantly increased the rate of shoot multiplication by the end of the third *** maximum mean number of shoots per explant (86.5±3.6) was produced after three multiplication cycles on 3 mg/L BA-supplemented *** vitro induced shoots were excised and rooted on half strength MS medium fortified with 0.25 mg/L indole-3-butyric acid (IBA) to obtain complete ***-regenerated plantlets obtained in vitro for the first time,were hardened and 95% survived under greenhouse conditions.

关键词： Boscia senegalensis Famine food Mature embryo Organogenesis

Overexpression of Brassica napus cytosolic fructose-1,6-bisphosphatase and sedoheptulose-1,7-bisphosphatase genes significantly enhanced tobacco growth and biomass

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2022年第1期21卷 49-59页

作者： LI Yan-yan GUO Li-na LIANG Cheng-zhen MENG Zhi-gang Syed TAHIRA GUO San-dui ZHANG Rui biotechnology Research Institute Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic ImprovementBeijing 100081P.R.China

Elevated activities of cytosolic fructose-1,6-bisphosphatase(cyFBPase) and sedoheptulose-1,7-bisphosphatase(SBPase)are associated with higher yields in plants. In this study, the expression levels of the cyFBPase and SBPase genes were increased by overexpressing rape(Brassica napus) cDNA in tobacco(Nicotiana tabacum) plants. The transgenic plants coexpressing cy FBPase and SBPase(TpFS), or expressing single cy FBPase(TpF) or SBPase(TpS) had 1.77-, 1.55-, 1.23-fold cyFBPase and 1.45-, 1.12-, 1.36-fold SBPase activities as compared to the wild-type(WT), respectively. Photosynthesis rates of TpF, TpS and TpFS increased 4, 20 and 25% compared with WT plants. The SBPase and cyFBPase positively regulated each other and functioned synergistically in transgenic tobacco plants. In addition, the sucrose contents of the three transgenic plants were higher than that of WT plants. The starch accumulation of the TpFS and TpS plants was improved by 53 and 37%, but slightly decreased in TpF plants. Moreover, the transgenic tobacco plants harbouring SBPase and/or cyFBPase genes showed improvements in their growth, biomass, dry weight, plant height, stem diameter, leaf size,flower number, and pod weight. In conclusion, co-expression of SBPase and cyFBPase may pave a new way for improving crop yield in agricultural applications.

关键词： tobacco cytosolic fructose-1,6-bisphosphatase(cy FBPase) sedoheptulose-1,7-bisphosphatase(SBPase) overexpression transformation

OsEIL1 and OsEIL2,two master regulators of rice ethylene signaling,promote the expression of ROS scavenging genes to facilitate coleoptile elongation and seedling emergence from soil

在线全文

学校读者我要写书评

暂无评论

Plant Communications 2024年第3期5卷 93-105页

作者： Jinzhu Qiao Ruidang Quan Juan Wang Yuxiang Li Dinglin Xiao Zihan Zhao Rongfeng Huang Hua Qin biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China national Key Facility of Crop Gene resources and genetic Improvement Beijing 100081China

Successful emergence from the soil is a prerequisite for survival of germinating seeds in their natural *** rice,coleoptile elongation facilitates seedling emergence and establishment,and ethylene plays an important role in this ***,the underlying regulatory mechanism remains largely ***,we report that ethylene promotes cell elongation and inhibits cell expansion in rice coleoptiles,result-ing in longer and thinner coleoptiles that facilitate seedlings emergence from the *** analysis showed that genes related to reactive oxygen species(ROS)generation are upregulated and genes involved in ROS scavenging are downregulated in the coleoptiles of ethylene-signaling *** investiga-tions showed that soil coverage promotes accumulation of ETHYLENE INSENSITIVE 3-LIKE 1(OsEIL1)and OsEIL2 in the upper region of the coleoptile,and both OsEIL1 and OsEIL2 can bind directly to the promoters of the GDP-mannose pyrophosphorylase(VTC1)gene OsVTC1-3 and the peroxidase(PRX)genes OsPRX37,OsPRX81,OsPRX82,and OsPRX88 to activate their *** leads to increased ascorbic acid content,greater peroxidase activity,and decreased ROS accumulation in the upper region of the *** of ROS accumulation promotes coleoptile growth and seedling emergence from *** deepen our understanding of the roles of ethylene and ROS in controlling coleoptile growth,and this information can be used by breeders to produce rice varieties suitable for direct seeding.

关键词： ethylene ROS coleoptile growth emergence rate rice

Female competition for availability of males in insects:the Nezara viridula(Linnaeus,1758)model

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2020年第4期27卷 801-814页

作者： Andrej Cokl Alenka Zunic Kosi Raul Alberto Laumann Meta Virant-Doberlet Department of Organisms and Ecosystems Research National Institute of BiologyLjubljanaSI-1000Slovenia Semiochemicals Laboratory Embrapa Genetic Resources and BiotechnologyBrasiliaDF 71070Brazil

Multimodal communication in solitary stinkbugs enables them to meet,mate and *** plant-dwelling species exchange information during the calling phase of mating behavior using substrate-borne vibratory signals.A female-biased gender ratio induces rivalry and competition for a sexual *** competition for males,first described among Heteroptera in three stinkbug species,revealed species specific differences and opened the question of plasticity in individually emitted temporal and frequency signal characteristics during calling and rival *** address this question and gain an insight into the mechanisms underlying stinkbug female rivalry,we compared the characteristics of alternated signals in the southern green stinkbug Nezara viridula(Linnaeus,1758)(Hemiptera:Pentatomidae).Compared to male rivalry,female rivalry is more complex,lasts longer and runs through successive phases by a combination of different song *** male pheromone triggers alternation between females,producing song pulses that occasionally overlap each *** female initiates the rivalry by changing individual pulses into pulse trains of three different *** competing female alternates with pulses of changed temporal characteristics at lower levels of rivalry and by varying the frequency characteristics of pulse trains at higher *** female rivalry,the male either stops responding or occasionally emits calling and courtship signals in response to the female that has produced signals of steady temporal *** rivalry shows complex and species specific patterns of information exchange at different levels with a broad-range variation of temporal and frequency characteristics of,until now,unidentified vibratory emissions.

关键词： biotremology female social conflict Nezara viridula rivalry signal discrimination

Scarabaeid Larvae- and Herbicide-Resistant Transgenic Perennial Ryegrass (Lolium perenne L.) Obtained by Agrobacterium tumefaciens-Mediated Transformation of cry8Ca2, cry8Ga and bar Genes

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2012年第1期11卷 53-61页

作者： WU Jin-xia ZHANG Zhi-guo ZHANG Qian LANG Zhi-hong SUN Xue-hui biotechnology Research Institute National Key Facility for Crop Gene Resources and Genetic ImprovementChinese Academy of Agricultural SciencesBeijing 100081P.R.China

Insect pest and weeds are two major problems for forage and turf grasses. In this study, scarab larvae- and herbicide-resistant transgenic perennial ryegrass （Lolium perenne L.） was obtained by transforming it with cry and bar genes simultaneously via the Agrobacterium-mediated method. To optimize the callus induction and plant regeneration conditions, various concentrations of 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine were assayed. The transformation efficiencies of different Agrobacterium suspension media, used during Agrobacterium-mediated transformation, were compared. Then, plasmids of pCAMBIA3301 containing cry gene （cry8Ca2 or cry8Ga） and bar gene, driven by ubiquitin promoter, were transformed into perennial ryegrass. The transformants were generated and confirmed by both Southern hybridization analysis and Western hybridization analysis. Further, the resistance of transgenic perennial ryegrass plants to scarab larvae and herbicide were analyzed. After 30 d of co-cultivation with scarab larvae, the damage to the root system of transgenic plants was less than that of non-transgenic control plants. Additionally, the leaves of transgenic plants were resistant to Basta, while leaves of the wild plants wilted after Basta spraying. These results show that cry gene and bar gene were successfully transferred into perennial ryegrass by the Agrobactgerium-mediated method, and convey resistance to scarab larvae and herbicide in transgenic perennial ryegrass plants.

关键词： Agrobacterium tumefaciens-mediated transformation transgenic perennial ryegrass scarab larvae resistance herbicide resistance