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环境昆虫学报 2011年第4期33卷 423-428页

作者： Gupta Tripti national bureau of agriculturally important Insects (ICAR)

Blaptostethus pallescens Poppius (Heteroptera:Anthocoridae),which is amenable to mass rearing,has proved to be a potential predator of sucking pests like mites and ***,the cotton mealybug Phenacoccus solenopsis Tinsley emerged as a serious pest on cotton in *** studies indicated that *** could feed on the cotton ***,further investigations were conducted to understand the stage of the predator which is most potential and the pest stage preferred by the *** nymphs and adults of *** could predate equally well on the neonates of cotton *** (three to four-day-old) nymphs of the predator could not feed on any stage of the cotton *** nymphs (seven-day-old and above) and adults could effectively predate on both young and mature cotton mealybug crawlers,though preference was more for the younger ***,nymphs and adults of *** could not predate on the adult stage of the *** predator:pest ratios were tested in cage studies and it emerged that *** adults released against the mealybug crawlers at a ratio of 1:5 and nymphal release at 1:10 caused maximum mortality of the mealybug *** the fast multiplication rate of cotton mealybug and the large number of predators which have to be released to manage them,further detailed studies are necessary on utilizing *** to target the neonates and younger stages of the cotton mealybug in field conditions.

关键词： Anthocoridae Blaptostethus pallescens feeding potential host stage preference mealybug Phenacoccus solenopsis

Production protocol for and storage efficacy of an anthocorid predator Cardiastethus exiguus Poppius

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环境昆虫学报 2012年第1期34卷 50-56页

作者： BALLAL Chandish R. national bureau of agriculturally important Insects(ICAR) India

Cardiastethus exiguus Poppius is an indigenous anthocorid predator of eggs and neonates of the notorious pest,coconut black-headed caterpillar Opisina arenosella Walker in *** the national bureau of agriculturally important Insects(Indian Council of Agricultural Research),Bangalore,India,a simple mass production protocol was developed for multiplying *** using UV-irradiated eggs of alternate laboratory host Corcyra cephalonica *** evaluation of the predator in the states of Kerala and Karnataka indicated that this predator could bring about a significant reduction in the pest ***,the need was felt to investigate the storage efficacy of the eggs and adults of *** so that sufficient numbers could be accumulated and transportation of the predator could be planned for field *** temperature storage studies indicated that *** eggs can be safely stored for up to 5 days at 10℃ and 10 days at 15℃ and incubation period could be staggered for up to 10 and 13 days,*** longevity of the *** adults was significantly reduced due to low temperature ***,for adult females,a storage temperature of 15℃ for 15 days could be recommended as they could live for a more than a month after removal from storage and their progeny production was comparable to that of the control adults.

关键词： Anthocoridae Cardiastethus exiguus coconut black-headed caterpillar Opisina arenosella production protocol storage

Prospecting microbial biofilms as climate smart strategies for improving plant and soil health: A review

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Pedosphere 2023年第1期33卷 129-152页

作者： Kulandaivelu VELMOUROUGANE Shobit THAPA Radha PRASANNA Division of Crop Production Indian Council of Agricultural Research-Central Institute for Cotton Research(CICR)Nagpur 440010India Indian Council of Agricultural Research-national bureau of agriculturally important microorganisms KushmaurMau Nath Bhanjan 275103India Division of Microbiology Indian Council of Agricultural Research-Indian Agricultural Research InstituteNew Delhi 110012India

Microbes and their products play key roles in complementing chemical fertilizers and plant protection chemicals by eliciting defence mechanisms in crop plants,thereby providing immunity and resistance against diverse *** the different environmental technologies used to mitigate climate change,several microbiological interventions are promising,among which biofilms,both naturally-existing and laboratory-developed,and their application have gained momentum *** biofilms are aggregations of microbial cells in a self-generated polymeric matrix,which are produced by several genera of bacteria,yeasts,cyanobacteria,and fungi as a mode of reproductive fitness,thereby advantageous in successful proliferation,even in extreme *** propensity of microbial biofilms to grow in diverse niches and adapt to biotic and abiotic stresses illustrates their immense potential as biofertilizers and disease suppression options in various crops across diverse *** the inhospitable habitats of deserts and denudated land,biofilms play an important role in preventing soil erosion and sustaining vegetation,microflora,and *** represent a mode of growth for several microbes with plant growth-promoting and biocontrol potential,which are important in seed establishment and the facilitation of exchanging nutrients and metabolites from the *** this review,we discuss the prospects of microbial biofilms as a green option in agriculture in general and,more specifically,their potential in mitigating climate change.

关键词： biocontrol bioremediation climate change environmental stress nutrient sequestration plant growth promotion

Biodiversity,current developments and potential biotechnological applications of phosphorus-solubilizing and-mobilizing microbes:A review

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Pedosphere 2021年第1期31卷 43-75页

作者： Divjot KOUR Kusam Lata RANA Tanvir KAUR Neelam YADAV Ajar Nath YADAV Manish KUMAR Vinod KUMAR Harcharan Singh DHALIWAL Anil Kumar SAXENA Department of Biotechnology Dr.Khem Singh Gill Akal College of AgricultureEternal UniversityBaru SahibSirmour 173101(India Gopi Nath P.G.College Veer Bahadur Singh Purvanchal UniversityGhazipur 275201(India Amity Institute of Biotechnology Amity UniversityGwalior 474005(India Biochemistry Forage SectionCollege of AgricultureCCS Agricultural UniversityHaryana 125004India ICAR-national bureau of agriculturally important microorganisms Kusmaur 275103(India)

As one of the most important and essential macronutrients next to nitrogen,phosphorus(P)is important for plant development,but it is the least mobile nutrient element in plant and ***,P is mined from geological sediments and added to agricultural soils so as to meet the critical requirements of crop plants for agronomic *** exists in soil in both organic and inorganic *** various inorganic forms of the element in soil are salts with calcium,iron,and aluminum,whereas the organic forms come from decaying vegetation and microbial *** is a huge diversity of plant microbiomes(epiphytic,endophytic,and rhizospheric)and soil microbiomes that have the capability to solubilize the insoluble P and make it available to *** main mechanism for the solubilization of inorganic P is by the production of organic acids,which lowers soil pH,or by the production of acid and alkaline phosphatases,which causes the mineralization of organic *** P-solubilizing and-mobilizing microorganisms belong to all three domains,comprising archaea,bacteria,and *** strains belonging to the genera Arthrobacter,Bacillus,Burkholderia,Natrinema,Pseudomonas,Rhizobium,and Serratia have been reported as efficient and potential P *** use of P solubilizers,alone or in combination with other plant growth-promoting microbes as an eco-friendly microbial consortium,could increase the P uptake of crops,increasing their yields for agricultural and environmental sustainability.

关键词： crop improvement gene expression microbial diversity organic acid production P mineralization P solubilizer phosphatase production plant growth-promoting traits

Induced Accumulation of Polyphenolics and Flavonoids in Cyanobacteria under Salt Stress Protects Organisms through Enhanced Antioxidant Activity

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American Journal of Plant Sciences 2014年第5期5卷 726-735页

作者： Dhananjaya P. Singh Ratna Prabha Kamlesh K. Meena Lalan Sharma Arun K. Sharma national bureau of agriculturally important microorganisms Indian Council of Agricultural Research Kushmaur Maunath Bhanjan 275103 India national Institute of Abiotic Stress Management Indian Council of Agricultural Research Baramati Pune 413115 India

Induced accumulation of polyphenolics and flavonoids in cyanobacterial strains grown under different salt concentrations is correlated with their growth under stress conditions and enhanced antioxidant activity. Plectonema boryanum, Hapalosiphon intricatus, Anabaena doliolum and Oscillatoria acuta grown for 21 days under different salt concentrations (80, 160, 240, 320 and 400 mM) in BG11 medium showed differential growth responses in terms of biomass, total protein, chlorophyll content, total content of polyphenol, flavonoid and carotenoid, accumulation of phenolic acids (gallic, caffeic, chlorogenic, ferullic and vanillic) and flavonoids (rutin and quercetin). Cyanobacterial extracts showed prominent free radical scavenging antioxidant activity (AOA) in terms of % DPPH discoloration. Highly significant (p < 0.05) and strong correlation was found between TPC and AOA (r = 0.974). Other positive but non-significant (p < 0.05) correlations were observed between AOA and gallic acid (r = 0.893) and AOA and caffeic acid (r = 0.931). Significant and strong correlation was also observed between gallic and caffeic acid (r = 0.973). Positive but lesser magnitude correlations were recorded between TPC and caffeic acid (r = 0.905), TPC and gallic acid (r = 0.920), gallic and vanillic acid (r = 0.916) and caffeic and vanillic acid (r = 0.814). An integrated combination of growth parameters, salt-induced accumulation of phenylpropanoids and stress-derived subsequent antioxidant property of cyanobacterial extracts is thought to provide evidence that secondary metabolic changes can act as the possible alternative mechanism to overcome stress-induced damages in cells.

关键词： Cyanobacteria Phenylpropanoids Flavonoids Antioxidants Stress Tolerance

Control of petroleum-hydrocarbon contaminated groundwater by intrinsic and enhanced bioremediation

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Journal of Environmental Sciences 2010年第6期22卷 864-871页

作者： Chih-Ming Kao Rao Y.Surampalli Mu-Sheng Lee Institute of Environmental Engineering "National" Sun Yat-Sen University U.S.Environmental Protection Agency Environmental Protection bureau Kaohsiung City Government

In the first phase of this study, the effectiveness of intrinsic bioremediation on the containment of petroleum hydrocarbons was evaluated at a gasoline spill site. Evidences of the occurrence of intrinsic bioremediation within the BTEX （benzene, toluene, ethylbenzene, and xylenes） plume included （1） decreased BTEX concentrations; （2） depletion of dissolved oxygen （DO）, nitrate, and sulfate; （3） production of dissolved ferrous iron, methane, and CO2; （4） deceased pH and redox potential; and （5） increased methanogens, total heterotrophs, and total anaerobes, especially within the highly contaminated areas. In the second phase of this study, enhanced aerobic bioremediation process was applied at site to enhance the BTEX decay rates. Air was injected into the subsurface near the mid-plume area to biostimulate the naturally occurring microorganisms for BTEX biodegradation. Field results showed that enhanced bioremediation process caused the change of BTEX removal mechanisms from anaerobic biodegradation inside the plume to aerobic biodegradation. This variation could be confirmed by the following field observations inside the plume due to the enhanced aerobic bioremediation process：（1） increased in DO, CO2, redox potential, nitrate, and sulfate, （2） decreased in dissolved ferrous iron, sulfide, and methane, （3） increased total heterotrophs and decreased total anaerobes. Field results also showed that the percentage of total BTEX removal increased from 92% to 99%, and the calculated total BTEX first-order natural attenuation rates increased from 0.0092% to 0.0188% per day, respectively, after the application of enhanced bioremediation system from the spill area to the downgradient area （located approximately 300 m from the source area）.

关键词： petroleum hydrocarbon groundwater natural attenuation intrinsic bioremediation

ECOLOGICAL DISTRIBUTION OF HYDROCARBON-DEGRADATION BACTERIA IN XIAMEN HARBOR

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Acta Oceanologica Sinica 1984年第1期 129-136页

作者：倪纯治周宗澄曾活水李志棠谢锦祥张南峰 Xiamen national bureau of Oceanography Third Institute of Oceanography

The ecology of hydrocarbon-degrading bacteria were investigated during five cruises in Xiamen Harbor. The results demonstrated that number of hydrocarbon-degrading bacteria were 2.1×102-7.5×106 cell/1 and 1.7×102-1.5×106 ceil/g dry and hetcrotrophic bacteria were 3.0× 104-5.9× 109 cell/1 and 2.53× 103-5.0× 103 cell/g dry in seawater and sediment respectively. The isolated strains which can degrade the petroleum belong to fifteen genera. Most, strains can only degsade one kind of hydro- carbon or petroleum. The results showed that the population and the species-composition of hydro carbon-degrading microorganisms were positively correlated with existing level of oil pollution and with water temperature, but independent of total microbtat count.

关键词： ECOLOGICAL DISTRIBUTION OF HYDROCARBON-DEGRADATION BACTERIA IN XIAMEN HARBOR

Characterization of a Bacteriocin-Like Substance Produced from a Novel Isolated Strain of Bacillus subtilis SLYY-3

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Journal of Ocean University of China 2014年第6期13卷 995-999页

作者： LI Junfeng LI Hongfang ZHANG Yuanyuan DUAN Xiaohui LIU Jie College of Chemical Engineering Qingdao University of Science & Technology Yantai Entry-Exit Inspection and Quarantine bureau

In the present research, the strain SLYY-3 was isolated from sediments of Jiaozhou Bay, Qingdao, China. The strain SLYY- 3, which produced a bacteriocin-like substance （BLS）, was characterized to be a strain of Bacillus subtillis by biochemical profiling and 16S rDNA sequence analysis. It is the first time to report that Bacillus subtilis from Jiaozhou Bay sediments could produce a BLS. The BLS of B. subtillis SLYY-3 exhibited strong inhibitory activity against gram-positive bacteria （including Staphylococcus aureus and B. subtillis） and some fimgi （including Penicillium glaucum, Aspergillus niger and Aspergillus flavus）. The antimicrobial activity was detected from culture in the exponential growth phase and reached its maximum when culture entered into stationary growth phase. It was thermo-tolerant even when being kept at 100~C for 60 min without losing any activity and stable over a wide pH range from 1.0 to 12.0 while being inactivated by proteolytic enzyme and trypsin, indicating the proteinaceous nature of the BLS. The BLS was purified by precipitation with hydrochloric acid （HC1） and gel filteration （Sephadex G-100）. SDS-PAGE analysis of the extracellular peptides of SLYY-3 revealed a bacteriocin-like protein with a molecular mass of 66 kDa. Altogether, these characteristics indicate the potential of the BLS for food industry as a protection against pathogenic and spoilage microorganisms.

关键词： bacteriocin-like substance Bacillus subtillis SLYY-3 antimicrobial activity food protection purification

Microbial community composition and environmental response characteristics of typical brackish groundwater in the North China Plain

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China Geology 2023年第3期6卷 383-394页

作者： Huai-sheng Zhang Wu-tian Cai Feng Guo Chao Bian Fu-dong Liu Lei Zhang Jin-wei Liu Miao Zhao Center for Hydrogeology and Environmental Geology Survey CGSBaoding 071051China national Research Center for Geoanalysis Key Laboratory of Eco-GeochemistryMinistry of Natural ResourcesBeijing 10037China Third Hydrological Engineering Geological Brigade Hebei Bureau of Geology and Mineral ResourcesHengshui 053000China

To reveal the microbial community composition of regional shallow porous brackish groundwater and its response characteristics to groundwater environment,the first and second aquifers in Taocheng District,Hengshui City were selected,and 10 groundwater source samples were collected for hydrochemical analysis and microbial 16S RNA gene V4-V5 regional *** results showed that the shallow brackish groundwater in the study area is weakly alkaline and has high ion *** hydrochemical types are SO_(4)·Cl-Na·Mg type and HCO3·Cl-Na·Mg type as a *** spatial zonation of the abundance and diversity of groundwater microorganisms is *** number of endemic bacteria in groundwater from upstream,midstream to downstream is 11,135 and 22 respectively,with a total of 22 *** is the most dominant in groundwater level(38.82%-86.88%),and there are obvious differences in different *** the genus level,the main dominant species in each group and sample are Pseudomonas and *** terms of composition difference,Pseudohongiella,Pseudorhodobacter and Limnohabitans are the representatives of UR,MR and *** the whole,the composition of flora in groundwater in the study area is sensitive and closely related to hydrochemical *** abundance is affected by alkaline and high salinity environmental indicators,while species diversity is related to depth and dissolved oxygen in weak reduction environment.

关键词： Groundwater microorganism Microbial Endemic bacteria Biochemistry Pseudomonas Hydrogenophage Brackish groundwater Environmental response Hydrogeological survey engineering Environmental geological survey engineering

Effects of lead pollution on soil microbial community diversity and biomass and on invertase activity

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Soil Ecology Letters 2023年第1期5卷 118-127页

作者： Xin Sun Mingjie Sun Ying Chao Xiaoyang Shang Hui Wang Hong Pan Quangang Yang Yanhong Lou Yuping Zhuge national Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources College of Resources and EnvironmentShandong Agricultural UniversityTai’an 271018China Tai’an Hi-Tech Industrial Development Zone Tai’an 271000China Rizhao Municipal bureau of Agricultural and Rural Affairs Rizhao 276800China Key Laboratory of Technologies and Models for Cyclic Utilization from Agricultural Resources Ministry of Agriculture and Rural AffairsBeiJing 100125China

Lead(Pb)pollution is one of the most widespread and harmful environmental problems *** of changes in soil properties and microbial functional diversity due to land use is needed to establish a basis for remediation of soil *** study aimed to investigate soils contaminated by Pb from different sources and to analyze the functional diversity and metabolism of soil microbial communities using Biolog *** pollution(>300 mg kg-1)significantly influenced the diversity and metabolic functions of soil microbial ***,Pb contamination significantly reduced soil microbial biomass carbon(C)and nitrogen(N)levels and catalase activity while increasing invertase ***,Biolog EcoPlate assays revealed that Pb pollution reduced the general activities of soil microorganisms,suppressing their ability to utilize C *** Pb-contaminated areas lacking vegetation cover,Shannon,Simpson,and McIntosh diversity indices of soil microorganisms were significantly *** microbial diversity and biomass C and N levels were affected by land use and soil properties,respectively,whereas soil enzyme activity was primarily affected by the interaction between land use and soil *** results provide a reference and a theoretical basis for developing soil quality evaluation and remediation strategies.

关键词： Lead pollution Biolog Microbial functional diversity Soil enzyme activities Environment toxicity