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Establishment of Recombinase Polymerase Amplification for Rapid Detection of Spring Viremia of Carp Virus(SVCV)

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Animal Husbandry and Feed Science 2020年第2期12卷 29-32页

作者： Liang Junni Yin Weili Shang Di Liu Ning Duan Xiaohui Lin Sen Technology center of Yantai Customs Yantai 264000China

[Objective]The paper was to develop a rapid method for the detection of spring Viremia of carp virus(SVCV).[Method]The specific primers were designed by targeting the G gene of *** recombinase polymerase amplification(RPA)assay for detecting SVCV was estab-lished by optimizing the reaction *** optimal amplification temperature of RPA assay was 30℃,and the test could be finished within 20 min.[Result]The method was specific with no cross-reaction with other common fish infectious *** test showed that the lowest detection limit of the method was 89.2 copies/μL,higher than that of traditional ***,a total of 80 clinical samples were detected by RPA and RT-PCR,*** weak positive samples tested by RT-PCR could be detectable with RPA,indicating that RPA assay could be used in clinical detection.[Conclusion]The method established is rapid,simple,specific and sensitive for testing SVCV,and it will be widely used in grassroots laboratory and on-site inspection.

关键词： Recombinase polymerase amplification Rapid detection Spring viremia of carp virus(SVCV)

Data Analysis for High Throughput Detection of 18 Phthalates in Textiles Based on High Resolution Mass Spectrometry

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Journal of Donghua University(English Edition) 2019年第2期36卷 158-162页

作者： QU Lianyi LUO Xin NIU Zengyuan YE Xiwen ZHANG Li TANG Zhixu ZHANG Lin ZHU Ping College of Textiles&Clothing Qingdao UniversityQingdao 266000China Inspection and Quarantine Technical center Qingdao CustomsQingdao 266002China

Liquid chromatography coupled to high resolution mass spectrometry(LC-HRMS)was used to establish a high throughput and sensitive method for 18 phthalic acid esters(PAEs)in *** method was applied to screen PAEs in 413 textile samples and 7 434 pieces of test data were *** discussion was conducted about specific compounds,textile materials,detected value distribution and positive rate of ***-nine percent of samples were detected with PAEs,and no samples were detected beyond *** phthalate(DiBP),Dibutyl phthalate(DBP)and Di-2-ethylhexyl phthalate(DEHP)were detected with high frequency,and most samples were fabrics with coating and *** study can be used to reflect distribution of phthalate residues in textiles on the market and provide data support for risk assessment of PAEs for textile producers and consumers.

关键词： high resolution mass spectrometry textile phthalic acid esters PAEs data analysis

Screening of DNA Barcode of Quarantine Phytophthora

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Agricultural Biotechnology 2017年第1期6卷 46-51,54页

作者： Ruifang GAO Yinghui CHENG Ying WANG Ying WANG Liyun GUO Guiming ZHANG Animal and Plant Inspection and Quarantine Technology center Shenzhen Entry-Exit Inspection and Quarantine Bureau Key Laboratory of Exotic Pest Detection Technology Research of Shenzhen College of Agriculture and Biotechnology China Agricultural University

Phytophthora is genus of plant-damaging Oomycetes, whose member species are capable of causing enormous economic losses on crops worldwide. In the present study, four candidate genes ITS, CO1, EF-1α and β-tubulin were tested using 123 strains of 80 species of Phytophthora to investigate the feasibility of ser- ving as DNA barcoding markers. The results showed that among the four candidate genes, ITS and CO1 had the highest success rate of PCR amplification and se- quencing, up to 100% and 96.7%. There were obvious barcode gaps in ITS, CO1 andβ-tubulin, but their frequency distributions of intra- and interspecific genetic distances were slightly overlapped. Wilcoxon rank sum test on intraspecific genetic distances of the four genes showed ITS = CO1 = β - tubulin = EF-1α indicating they bad the same effect on intraspecific discrimination, while the test on interspecific genetic distances of the four genes showed ITS 〉 C01 〉 β- tubulin 〉 EF - 1α. In summary, ITS and COl should be used in combination as the primary barcodes, β-tubulin as the complementary barcede for the identification of 11 quaran- tine Phytophthora species.

关键词： Quarantine Phytophthora DNA barcoding Genetic distance Barcoding gap

Expert consensus on the technical specifications of the in vitro skin penetration test of cosmetic product by Franz diffusion cell

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Journal of Dermatologic Science and Cosmetic Technology

Journal of Dermatologic Science and Cosmetic Technology 2024年第3期1卷 79-85页

作者： Bingwen Liang Liling Zhou Chuanbin Wu Liang Fang Lisheng Wang Lihua Peng Peng Shu Lifeng Tang Yu Bei Xiaodong Cui Xiaojia Chen Yuan Gao Chong Han Yan Jia Huaqing Lim Yifang Li Ying Liu Yiguang Li Zhiwei Li Zixiu Liu Shaohua Ren Jun Shi Wanyang Sun Lijuan Tian Qun Wang Yuan Wang Yuehong Xu Ming Zeng Quangang Zhu Shu Zhang Yifan Zhang Qi Xiang The transdermal Drug Delivery Committee of the World Federation of Chinese Medicine Societies Beijing 100020China Guangzhou University of Chinese Medicine Guangzhou 510006China Jinan University Guangzhou 510632China Shenyang Pharmaceutical University Shenyang 110016China Zhejiang University Hangzhou 310058China Shenzhen Hujia Technology Co. Ltd.Shenzhen 518066China Guangzhou Xika Technology Co. Ltd.Guangzhou 510653China Guangzhou Kingtest Technology Co. Ltd.Guangzhou 510663China China Council for the Promotion of national Trade Beijing 100020China Fudan University Shanghai 200433China Guangdong Lewwin Pharmaceutical Research Institute Co. Ltd.Guangzhou 510900China Beijing Technology and Business University Beijing 10048China Guangdong Pharmaceutical University Guangzhou 510006China Heizhimei(Guangzhou)Biotechnology Co. Ltd.Guangzhou 510700China Jinan University Cosmetics and Medical Industry Alumni Association Guangzhou 510000China Novozen Technology(Shanghai)Co. Ltd.Shanghai 200120China Sun Yat-Sen University Zhuhai 510275China The Sixth Affiliated Hospital Sun Yat-sen University Guangdong Gastrointestinal HospitalGuangzhou 510655China Shanghai Skin Diseases Hospital Shanghai 200443China Biopharmaceutical R&D center of Jinan University Guangzhou 510665China

Background:Diffusion cell test is one of the most commonly used in vitro model to detect the percutaneous absorption of cosmetic *** PRC State Administration of quality supervision and Quarantine has issued the guideline for the in vitro test method for the percutaneous absorption of drug molecules,there is still a lack of guideline for the percutaneous absorption test of cosmetic ***:In order to improve the standardized level and make the in vitro percutaneous absorption test of cosmetic ingredients more comparable,based on the international and domestic experience,Transdermal Drug Delivery Committee of the World Federation of Chinese Medicine Societies organized the experts to discuss and formed this consensus as reference for the percutaneous absorption test of cosmetic *** and Methods:To standardize the in vitro diffusion cell test technique for transdermal penetration of functional cosmetic ingredients,the Transdermal Drug Delivery Professional Committee of the World Federation of Societies of Traditional Chinese Medicine has organized many discussions among experts.

关键词： Cosmetics Percutaneous absorption Diffusion cell Expert consensus