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SARS-CoV-2 ORF8 does not function in the nucleus as a histone mimic

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Protein & Cell 2024年第2期15卷 79-82页

作者： Ping Liu Junjie Hui Lei Wang national Laboratory of biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing100101China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China

Disruption of epigenetic regulation in host cells is a common evasion strategy used by viruses(Ozturkler and Kalkan 2021).Recently,Kee et al.(2022)reported that accessory protein ORF8 of SARS-CoV-2 can diffuse into the cell nucleus and mimic histone H3 through the ARKS motif,thereby interfering with H3 post-translational modifications(PTMs),disrupting gene transcription,and diminishing the antiviral response.

关键词： mimic thereby function

Engineering The Neck Hinge Reshapes The Processive Movement of Kinesin-3

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生物化学与生物物理进展 2024年第10期51卷 2730-2740页

作者： LI Dong REN Jin-Qi SONG Yin-Long LIANG Xin FENG Wei Key Laboratory of biomacromolecules(CAS) National Laboratory of BiomacromoleculesCAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China School of Life Sciences Tsinghua UniversityBeijing 100084China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China

Objective In kinesin-3,the neck coil correlates with the following segments to form an extended neck that contains a characteristic hinge diverse from a proline in KIF13B to a long flexible linker in *** function of this neck hinge for controlling processive movement,however,remains *** We made a series of modifications to the neck hinges of KIF13B and KIF1A and tested their movement using a single-molecule motility *** In KIF13B,the insertion of flexible residues before or after the proline differentially impacts the processivity or velocity,while the removal of this proline increases the *** KIF1A,the deletion of entire flexible neck hinge merely enhances the *** engineering of these hinge-truncated necks of kinesin-3 into kinesin-1 similarly boosts the processive movement of *** The neck hinge in kinesin-3 controls its processive movement and proper modifications tune the motor motility,which provides a novel strategy to reshape the processive movement of kinesin motors.

关键词： intracellular transport molecular motor kinesin-3 neck hinge processive movement

Complicated target recognition by archaeal box C/D guide RNAs

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Science China(Life Sciences) 2024年第4期67卷 631-644页

作者： Jiayin Wang Songlin Wu Keqiong Ye Key Laboratory of RNA Science and Engineering CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Box C/D RNAs guide the site-specific formation of 2′-O-methylated nucleotides(Nm)of RNAs in eukaryotes and *** C/D RNAs have been profiled in several archaea,their targets have not been experimentally ***,we mapped Nm in r RNAs,t RNAs,and abundant small RNAs(s RNAs)and profiled C/D RNAs in the crenarchaeon Sulfolobus *** targets of C/D RNAs were assigned by analysis of base-pairing interactions,in vitro modification assays,and gene deletion experiments,revealing a complicated landscape of C/D RNA-target interactions.C/D RNAs widely use dual antisense elements to target adjacent sites in r RNAs,enhancing modification at weakly bound *** consecutive sites can be guided with the same antisense element upstream of box D or D′,a phenomenon known as doublespecificity that is exclusive to internal box D′in eukaryotic C/D *** C/D RNAs guide modification at a single non-canonical *** study reveals the global landscape of RNA-guided 2′-O-methylation in an archaeon and unexpected targeting rules employed by C/D RNA.

关键词： C/D RNA 2′-O-methylation archaea guide RNA RNA modification

Join the club:YIPF3 and YIPF4 act as Golgiphagy receptors

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Life Metabolism 2024年第1期3卷 7-9页

作者： Xiaoli Ma Hong Zhang national Laboratory of biomacromolecules New Cornerstone Science LaboratoryInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China

Autophagic degradation of membrane-bound organelles such as the endoplasmic reticulum(ER),mitochondria,and peroxisomes is mediated by a family of *** a recent study,Hickey et *** that autophagy triggered by nutrient stress,previously thought to degrade bulk cytoplasmic contents,prioritizes the ER and Golgi as cargoes for *** further demonstrated that Yip1 domain family member 3(YIPF3)and YIPF4 act as receptors to mediate the delivery of Golgi to the lysosomes for Golgi remodelling during nutrient stress and also neuronal differentiation.

关键词： PF4 reticulum

Deep learning-based optical aberration estimation enables offline digital adaptive optics and super-resolution imaging

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Photonics Research 2024年第3期12卷 474-484页

作者： CHANG QIAO HAOYU CHEN RUN WANG TAO JIANG YUWANG WANG DONG LI Department of Automation Tsinghua UniversityBeijing 100084China Institute for Brain and Cognitive Sciences Tsinghua UniversityBeijing 100084China national Laboratory of biomacromolecules New Cornerstone Science LaboratoryCAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China Beijing national Research Center for Information Science and Technology Tsinghua UniversityBeijing 100084China

Optical aberrations degrade the performance of fluorescence *** adaptive optics(AO)leverages specific devices,such as the Shack–Hartmann wavefront sensor and deformable mirror,to measure and correct optical ***,conventional AO requires either additional hardware or a more complicated imaging procedure,resulting in higher cost or a lower acquisition *** this study,we proposed a novel space-frequency encoding network(SFE-Net)that can directly estimate the aberrated point spread functions(PSFs)from biological images,enabling fast optical aberration estimation with high accuracy without engaging extra optics and image *** showed that with the estimated PSFs,the optical aberration can be computationally removed by the deconvolution ***,to fully exploit the benefits of SFE-Net,we incorporated the estimated PSF with neural network architecture design to devise an aberration-aware deeplearning super-resolution model,dubbed *** demonstrated that the combination of SFE-Net and SFT-DFCAN enables instant digital AO and optical aberration-aware super-resolution reconstruction for live-cell imaging.

关键词： optics resolution enable

Wnt/β-catenin Signaling Cascades in Cardiovascular Diseases

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生物化学与生物物理进展 2024年第10期51卷 2328-2339页

作者： QI Yan-Fei LIU Yan-Ping XU Wen-Qing YAN Xiao-Xue national Laboratory of biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China Shanghai Institute for Advanced Immunochemical Studies School of Life Science and TechnologyShanghai Tech UniversityShanghai 201210China

Cardiovascular diseases are a group of disorders of the heart and blood vessels,primarily including coronary heart disease,stroke,and other *** is the world’s leading cause of death,and its incidence is increasing *** is a major risk factor for cardiovascular *** signaling comprises a series of highly conservative cascading events controlling fundamental biological *** signaling pathways include the canonical Wnt pathway(or Wnt/β-catenin pathway),the non canonical planar cell-polarity pathway,and the non-canonical calcium-dependent *** Wnt signaling promotes cell proliferation and differentiation,cardiac malformations,various malignancies,so drugs targeting Wnt signaling play a great therapeutic ***/β-catenin pathway is involved in the occurrence and development of cardiovascular diseases such as atherosclerosis and stroke by regulating cell proliferation,migration,apoptosis,blood-brain barrier permeability,inflammation,oxidative stress,and immune *** on the latest research progress,this review summarizes the role of Wnt/β-catenin signaling in cardiovascular diseases,in order to provide new ideas for the prevention and treatment of cardiovascular diseases.

关键词： Wnt/β-catenin pathway cardiovascular diseases pathological process

CRL2^(APPBP2)-mediated TSPYL2 degradation counteracts human mesenchymal stem cell senescence

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Science China(Life Sciences) 2024年第3期67卷 460-474页

作者： Daoyuan Huang Qian Zhao Kuan Yang Jinghui Lei Ying Jing Hongyu Li Chen Zhang Shuai Ma Shuhui Sun Yusheng Cai Guibin Wang Jing Qu Weiqi Zhang Si Wang Guang-Hui Liu Advanced Innovation Centerfor Human Brain Protection National Clinical Research Centerfor Geriatric DisordersXuanwu Hospital Capital Medical UniversityBeijing 100053China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Aging Translational MedicineCenter International Centerfor Aging and CancerBejing Municipal GeriatricMedical Research CenterXuanwu HospitalCapital Medical UniversityBeijing 100053China State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Beijing Institute for Stem Cell and Regenerative Medicine Beijing100101China CAS Key Laboratory of Genomic and Precision Medicine Bejing Institute of Genomics and China National Center for BioinformationChinese Academy of SciencesBeijing 100101China Institute for Stem Cell and Regeneration CASBeijing 100101China The Fifth People's Hospital of Chongqing Chongqing 400062China Sino-Danish Cllege University of Chinese Academy of SciencesBeijing101408China national Laboratory of biomacromolecules CAS Center for Excelence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing100101China State Key Laboratory of Proteomics Beijing Proteome Research CenterNational Center for Protein Sciences(Beijing)Beijing Institute of LifeomicsBeijing 102206China

Cullin-RING E3 ubiquitin ligases(CRLs),the largest family of multi-subunit E3 ubiquitin ligases in eukaryotic cells,represent core cellular machinery for executing protein degradation and maintaining ***,we asked what roles Cullin proteins play in human mesenchymal stem cell(hMSC)homeostasis and *** this end,we conducted a comparative aging phenotype analysis by individually knocking down Cullin members in three senescence models:replicative senescent hMSCs,Hutchinson-Gilford Progeria Syndrome hMSCs,and Werner syndrome *** all family members,we found that CUL2 deficiency rendered hMSCs the most susceptible to *** investigate CUL2-specific underlying mechanisms,we then applied CRISPR/Cas9-mediated gene editing technology to generate CUL2-deficient human embryonic stem cells(hESCs).When we differentiated these into h MSCs,we found that CUL2 deletion markedly accelerates hMSC ***,we identified that CUL2 targets and promotes ubiquitin proteasome-mediated degradation of TSPYL2(a known negative regulator of proliferation)through the substrate receptor protein APPBP2,which in turn downregulates one of the canonical aging marker-P21^(waf1/cip1),and thereby delays *** work provides important insights into how CRL2^(APPBP2)-mediated TSPYL2 degradation counteracts hMSC senescence,providing a molecular basis for directing intervention strategies against aging and aging-related diseases.

关键词： Cullins stem cell senescence aging proteostasis ubiquitination APPBP2 TSPYL2

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破译pH值和金属离子编程的家蚕纺丝机制

Science Bulletin 2024年第6期69卷 792-802页

作者：宋凯王叶菁董文杰李珍珍夏庆友朱平何华伟 Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science City Biological Science Research CenterSouthwest UniversityChongqing 400715China national Laboratory of biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China Chongqing Key Laboratory of Sericultural Science Chongqing Engineering and Technology Research Center for Novel Silk MaterialsSouthwest UniversityChongqing 400715China University of Chinese Academy of Sciences Beijing 100049China Chongqing Key Laboratory of Soft-Matter Material Chemistry and Function Manufacturing Chongqing 400715China

Silk is one of the toughest fibrous materials known despite spun at ambient temperature and pressure with water as a *** is a great challenge to reproduce high-performance artificial fibers comparable to natural silk by bionic for the incomplete understanding of silkworm spinning in ***,we found that amphipol and digitonin stabilized the structure of natural silk fibroin(NSF)by a large-scale screening in vitro,and then studied the close-to-native ultrastructure and hierarchical assembly of NSF in the silk gland *** study showed that NSF formed reversible flexible nanofibrils mainly composed of random coils with a sedimentation coefficient of 5.8 S and a diameter of about 4 nm,rather than a micellar or rod-like structure assembled by the aggregation of globular NSF *** ions were required for NSF nanofibril *** successive p H decrease from posterior silk gland(PSG)to anterior silk gland(ASG)resulted in a gradual increase in NSF hydrophobicity,thus inducing the sol-gelation transition of NSF *** nanofibrils were randomly dispersed from PSG to ASG-1,and self-assembled into anisotropic herringbone patterns at ASG-2 near the spinneret ready for silkworm *** findings reveal the controlled self-assembly mechanism of the multi-scale hierarchical architecture of NSF from nanofibrils to herringbone patterns programmed by metal ions and p H gradient,which provides novel insights into the spinning mechanism of silk-secreting animals and bioinspired design of high-performance fibers.

关键词： Natural silk fibroin Nanofibril Self-assembly Silkworm Cryo-EM

The romance of replication:Hand in hand,from dawn to dusk

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Science Bulletin 2024年第16期69卷 2474-2475页

作者： Runze Ma Zijing Liu Bing Zhu national Laboratory of biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Key Laboratory of Epigenetic Regulation and Intervention Chinese Academy of SciencesBeijing 100101China New Cornerstone Science Laboratory Institute of BiophysicsChinese Academy of SciencesBeijing 100101China

Faithful DNA duplication is the fundamental event for all life *** to replicate the whole genome quickly and efficiently is a challenge that must be faced within every S phase of the cell cycle[1].Considering the increased size and complexity of mammalian genomes,it is plausible that the regulation of DNA replication is more intricate in *** of thousands of replication origins have been identified throughout mammalian genomes[2].Whether replication forks originated from different origins cooperate during replication is a long-term concern in the field.

关键词： originated field. replication