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Valorization of Asparagus leafy by-product by ionic-liquid extraction and characterization of bioactive co.pounds in the extracts

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Food Bioscience 2022年第2期46卷 857-864页

作者： Bimal Chitrakar Min Zhang Sakamon Devahastin Benu Adhikari Xiaohu Zhang State Key Laboratory of Food Science and technology Jiangnan University214122WuxiJiangsuChina co.lege of Food Science and technology Hebei Agricultural UniversityBaoding071001HebeiChina Jiangsu Province International Joint Laboratory on Fresh Food Smart Processing and Quality Monitoring Jiangnan University214122WuxiJiangsuChina Advanced Food Processing Research Laboratory Department of Food EngineeringFaculty of EngineeringKing Mongkut’s University of Technology Thonburi126 Pracha u-tid RoadBangkok10140Thailand School of Science RMIT UniversityMelbourneVIC3083Australia nantong shuangyang ecological agriculture technology co. Ltd.NantongJiangsuChina

Asparagus(Asparagus officinalis)is a high-value low-calorie vegetable with array of bioactive *** processing nevertheless generates substantial quantity of by-products;their valorization is of utmost importance for the sustainability of the ***,feasibility of using four imidazolium-based ionic liquids and their co.binations as well as water and methanol to extract bioactive co.pounds from asparagus leafy by-product was *** of ultrasound-microwave-assisted heating to augment extraction was also assessed.1-ethyl-3-methylimidazolium-bromide(EMIMBr)resulted in the highest extraction yield of rutin(25.7 mg/g),which is the most abundant co.pound in the by-product;such a yield was co.parable to that achievable via methanol ***-microwave-assisted extraction did not show significant improvement in rutin yield when EMIMBr was ***,although methanolic extract exhibited slightly higher co.tent of rutin,it also co.tained the higher number of impurities;EMIMBr extract co.tained less impurities with higher relative co.tent of rutin(70.9%)than that in methanolic extract(67.7%).This co.ld be a milestone in extraction work for an efficient purification.

关键词： 1-Ethy-3-methylimidazolium bromide Ionic-liquid Methanol-extraction Rutin Ultrasound-microwave extraction

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Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature

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Chinese Journal of Oceanology and Limnology 2015年第1期33卷 11-19页

作者：柳意樊马道远肖志忠徐世宏王彦丰王雨福肖永双宋宗诚滕照军刘清华李军 Institute of Oceanology Chinese Academy of Sciences University of Chinese Academy of Sciences nantong Branch Institute of OceanoIogyChinese Academy of Sciences Weihai Shenghang Aquatic Product Science and technology co.Ltd. Rizhao Proliferation Station of Marine Aquatic Resources

High temperature influences the homeostasis of fish. We investigated the effects of elevated temperature on tissues of Japanese flounder （Paralichthys olivaceus） by analyzing the histology and heat shock protein 70 （hsp70） expression of fish reared in warm co.ditions. In this study, temperature was increased at 1±0.5℃/day starting at 24±0.5℃, and was kept at that temperature for 5 days before the next rise. After raising temperature at the rate up to 32±0.5℃, tissue samples from midgut, spleen, stomach, liver, muscle, gill, heart, trunk kidney and brain were co.lected for histological analysis and mRNA assay. Almost all the tissues showed changes in morphological structure and hsp70 level at 32±0.5℃. Histological assessment of the tissues indicated that the gill had the most serious damage, including highly severe epithelial lifting and edema, curved tips and hyperemia at the ending of the lamellars, desquamation and necrosis. The next most severe damage was found in liver and kidney. The hsp70 levels in all the tissues first increased and then decreased. The gut, stomach, muscle, heart, and brain had the highest expressions in 6 h, whereas the spleen, liver, gill and kidney had the highest expressions in 2 h. Therefore, tissues with the most significant lesions （especially gill and liver） responded much earlier （2 h） in hsp70 expression than other tissues, and these tissues demonstrated the most marked histological disruption and elevated mRNA levels, making them ideal candidates for further studies on the thermal physiology of this species.

关键词： temperature Paralichthys olivaceus histology hsp70 tissue expression

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