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Ionic Liquid-Polypyrrole-Gold Composites as Enhanced Enzyme Immobilization Platforms forHydrogen Peroxide Sensing

北京日化 2019年第2期 15-32页

作者： Meng Li Jing Wu Haiping Su Yazhuo Shang Yifan He Honglai Liu Key laboratory for Advanced Materials School of Chemistry and Molecular Engineering East China University of Science and Technology Shanghai 200237 China Department of Biotechnology School of Sciences Beijing Technology and Business University Beijing 100048 China

In this work, three different aqueous solutions containing imidazole-based ILs w让h different alkyl chain lengths ([Cnmim]Br, n = 2Z 6,12) were adopted as the medium for the synthesis of ionic liquid-polypyrrole (IL-PPy) composites. Herein, the ILs undertook the roles of the pyrrole solvent, the media for emulsion polymerization of PPy and PPy dopants, respectively. The electrochemical performances of the three IL-PPy composites on a glassy carbon electrode (GCE) were investigated by electrochemical experiments, which indicated that [Ci2mim]Br-PPy (Ci2~PPy) composites displayed better electrochemical performance due to their larger surface area and firmer immobilization on the GCE. Further, Ci2?PPy/GCE were decorated with Au microparticles by electrodeposition that can not only increase the conductivity, but also immobilize sufficient biomolecules on the electrode. Then, the obtained Ci2~PPy^Au/GCE with outstanding electrochemical performance was employed as a horseradish peroxidase (HRP) immobilization platform to fabricate a novel Ci2-PPy-Au-HRP/GCE biosensor for H2O2 detection. The results showed that the prepared Ci2-PPy-Au-HRP/GCE biosensor exhibited high sensitivity, fast response, and a wide detection range as well as low detection limit towards H2O2. This work not only provides an outstanding biomolecule immobilization matrix for the fabrication of highly sensitive biosensors, but also advances the understanding of the roles of ILs in improving the electrochemical performance of biosensors.

关键词： ionic liquids (ILs) polypyrrole (PPy) gold particles horseradish peroxidase (HRP) enzyme immobilization hydrogen peroxide sensor

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拉洛他赛的波谱解析及高效液相色谱法对其脂质体含量的测定

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北京大学学报（医学版） 2019年第3期51卷 467-476页

作者：李雪琦李建伟李秋红阎妍段嘉伦崔一诺苏展博罗倩许佳瑞杜亚菲王桂玲谢英吕万良天然药物与仿生药物国家重点实验室分子药剂学与新释药系统北京市重点实验室北京大学药学院北京100191 《中国药学(英文版)》 Asian Journal of Pharmaceutical Sciences Precision nanomedicine BioMed Research International 北京大学前沿交叉学科研究院北京100871 山西振东制药股份有限公司制剂所山西长治047100 山西大学中医药现代研究中心太原030006 中国药学会药剂专业委员会中国颗粒学会、生物颗粒专业委员会《北京大学学报(医学版)》《中国药学杂志》《中国新药杂志》

目的:拉洛他赛是国内外均未上市的新结构药物,未见质量研究相关报道。对拉洛他赛进行波谱解析以验证其分子式、相对分子质量和化学结构式,同时建立一种定量方法用于拉洛他赛脂质体制剂的含量测定。方法:利用质谱、红外吸收光谱、核磁共... 详细信息

目的:拉洛他赛是国内外均未上市的新结构药物,未见质量研究相关报道。对拉洛他赛进行波谱解析以验证其分子式、相对分子质量和化学结构式,同时建立一种定量方法用于拉洛他赛脂质体制剂的含量测定。方法:利用质谱、红外吸收光谱、核磁共振波谱测定方法,对拉洛他赛进行药物结构和光谱学解析;利用紫外-可见分光光度法对拉洛他赛进行全波长扫描,确定其吸收波长;利用高效液相色谱法,建立拉洛他赛定量方法并用于脂质体拉洛他赛包封率的测定。结果:揭示了拉洛他赛的四大光谱学特征并制订相应的标准图谱。确认了拉洛他赛的结构为三环二萜,分子式为C45H53NO14 ,相对分子质量为831.900 1。建立了拉洛他赛的高效液相色谱定量方法,其色谱柱为C18硅胶反相色谱柱(5 μm, 250 mm×4.6 mm),流动相为乙腈-水(体积比75 ∶ 25),检测波长为230 nm,该方法可用于测定脂质体制剂中拉洛他赛的包封率,稳定性、回收率和精密度高。此外,新制备了拉洛他赛脂质体,该脂质体粒径大约105 nm,均一性良好,药物包封率大于80%。结论:制订了拉洛他赛的质谱、红外吸收光谱、核磁共振波谱和紫外-可见光谱图谱,验证了拉洛他赛的分子式、相对分子质量和结构式,建立了拉洛他赛的高效液相色谱定量方法,该方法可用于拉洛他赛脂质体的质量控制。

关键词：拉洛他赛波谱解析高效液相色谱法

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FeeNeC single atom nanozymes with dual enzyme-mimicking activities for colorimetric detection of hydrogen peroxide and glutathione

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Journal of Materiomics 2022年第6期8卷 1251-1259页

作者： Wei Lu Shuhan Chen Haifeng Zhang Jiajun Qiu Xuanyong Liu State Key laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghai200050China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing100049China School of Chemistry and Materials Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou310024China

H_(2)O_(2) and glutathione(GSH)are critical redox molecules in the *** levels of cellular H_(2)O_(2) and GSH are closely related to some ***,it is imperative to detect H_(2)O_(2) and GSH *** this work,FeeNeC single atom nanozymes(SANs)with both peroxidase and oxidasemimicking activities were successfully prepared with the help of formamide condensation by one-step hydrothermal *** FeeNeC SANs show excellent peroxidase-like activity,which possess a higher affinity for H_(2)O_(2) and 3,30,5,50-tetramethylbenzidine(TMB)than horseradish peroxidase(HRP).Then,based on the chromogenic reaction of TMB,a colorimetric biosensor to detect H_(2)O_(2) and GSH was *** biosensor has the linear ranges of 10-600 mmol/L for H_(2)O_(2) with a low detection limit of 4.360 mmol/L and 100-400 mmol/L for GSH with a low detection limit of 78.33 mmol/***,this colorimetric biosensor exhibited a good recovery of H_(2)O_(2) and GSH in diluted human ***,the FeeNeC SANs were encapsulated into agar gel to self-quantitatively detect GSH by naked *** work provides a non-pyrolytic way to prepare SANs,which broadens the synthetic method of SANs and may promote the development of SANs for biosensor application.

关键词： Single atom nanozymes biosensor Peroxidase mimicking Hydrogen peroxide Glutathione

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A Simple and Reliable Assay for Detecting Specifi c Nucleotide Sequences in Plants Using Optical Thin-fi lm biosensor Chips

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分子植物育种 2007年第2期5卷 241-241页

作者： S. Bai　 X. Zhong　 L. Ma 　W. Zheng　 L. Fan N. Wei　 X.W. Deng Peking-Yale Joint Center for Plant Molecular Genetics and Agro-Biotechnology and The National Laboratory of Protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing100871 China Dopartment of Pharmacology Toxicology and Therapeutics University of Kansas Medical Center Kansas City KS 66160 USA Tianjin Custom Inspection and Quarantine Bureau of the People＇s Republic of China Tianjin 300201 China Department of Molecular Cellular and Developmental Biology Yale University New Haven CT 06520-8104 USA

Here we report the adaptation and optimization of an effi cient, accurate and inexpensive assay that employs custom-designed silicon-based optical thin-fi lm biosensor chips to detect unique transgenes in genetically ... 详细信息

关键词：核苷酸生物传感器植物实验研究

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