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The protective effect of dietary flavonoid fraction from Acanthophora spicifera on streptozotocin induced oxidative stress in diabetic rats

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Food Science and Human Wellness 2016年第2期5卷 57-64页

作者： Lavakumar Vuppalapati Ravichandiran Velayudam K.F.H.Nazeer Ahamed Sowmya Cherukuri Bhaskar Reddy Kesavan center for nanobiotechnology Sri Venkateswara College of PharmacyRVS NagarChittoor 517127APIndia NIPER Raja S.C.Mullick RoadJadavpurKolkata 700032India center for research and Innovation Asia Metropolitan UniversityCheras 43200Malaysia Department of Pharmaceutics RIPERAnantapuram 515721APIndia

The present investigation was considered in arraying of antidiabetic and antioxidant activity from dietary flavonoid loaded fraction of Acanthophora spicifera(***,Family:Rhodomelaceae)on streptozotocin(STZ)induced oxidative stress *** testings were acted upon male rats,which were alienated into five groups:control group,diabetic group(single dose of 65 mg/kg,streptozotocin(STZ)i.p.),diabetic with insulin(6 IU),and diabetic with flavonoid rich fraction groups(FRF)at 50 and 100 mg/kg body weight,given orally for 21 *** blood glucose level was determined at different week *** antioxidant consequences of FRF on STZ-induced diabetic rats were determined by the estimations of the oxidative stress marker like malonyldialdehyde and antioxidant enzymes such as superoxide dismutase,catalase and glutathione in tissue homogenates of heart,liver and *** treatment of diabetic rats significantly(P<0.05)diminishes the blood glucose altitudes to normal in contrast with diabetic ***,FRF administration,significantly decreased the malonyldialdehyde(MDA)and increased the activities of superoxide dismutase(SOD),catalase(CAT)and glutathione levels(GSH)in diabetic *** outcome designates that FRF fraction from red algae *** was potent anti diabetic and antioxidant asset against STZ induced diabetes and oxidative tissue breakups.©2016 Beijing Academy of Food *** and hosting by Elsevier *** rights reserved.

关键词： Acanthophora spicifera Red algae Streptozotocin Diabetes Antioxidant

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Probing surface hydrophobicity of individual protein at single-molecule resolution using solid-state nanopores

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Science China Materials 2015年第6期58卷 455-466页

作者：李骥唐智鹏胡蕊付强严尔富王绍英郭培宣赵清俞大鹏 State Key Laboratory for Mesoscopic Physics School of PhysicsPeking University Beijing research Institute of Chemical Industry nanobiotechnology center Markey Cancer Centerand Department of Pharmaceutical SciencesUniversity of Kentucky Collaborative Innovation center of Quantum Matter

Solid-state nanopore is found to be a promising tool to detect proteins and their complexes. Nanopore-protein interaction is a fundamental and ubiquitous process in biology and medical biotechnology. By translocating phi29 connector protein through silicon nitride nanopores, we demonstrate preliminarily probing the surface hydrophobicity of individual protein at single-molecule resolution. The unique 'double-level event' observed in the translocation and the ratio of two current drop levels suggest that the position where the interaction occurs is the hydrophobic surface of the protein. We provide a potential method to locate the hydrophobic region of a specific protein surface. This study is of fundamental significance in revealing the important role that hydrophobic interaction plays in nanopore-protein interaction and holds great potential for detecting local surface chemical property of individual protein using solid-state nanopores.

关键词：纳米孔疏水表面

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Targeted delivery of RNAi to cancer cells using RNA-ligand displaying exosome

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Acta Pharmaceutica Sinica B 2023年第4期13卷 1383-1399页

作者： Nasir Uddin Daniel WBinzel Dan Shu Tian-Min Fu Peixuan Guo center for RNA nanobiotechnology and Nanomedicine Division of Pharmaceutics and PharmacologyCollege of Pharmacythe Ohio State UniversityColumbusOH 43210USA Dorothy M.Davis Heart and Lung research Institute the Ohio State UniversityColumbusOH 43210USA James Comprehensive Cancer center College of Medicinethe Ohio State UniversityColumbusOH 43210USA Department of Biological Chemistry&Pharmacology College of Medicinethe Ohio State UniversityColumbusOH 43210USA

Exosome is an excellent vesicle for in vivo delivery of therapeutics,including RNAi and chemical *** extremely high efficiency in cancer regression can partly be attributed to its fusion mechanism in delivering therapeutics to cytosol without endosome ***,being composed of a lipidbilayer membrane without specific recognition capacity for aimed-cells,the entry into nonspecific cells can lead to potential side-effects and *** engineering approaches for targeting-capacity to deliver therapeutics to specific cells is *** with chemical modification in vitro and genetic engineering in cells have been reported to decorate exosomes with targeting *** nanoparticles have been used to harbor tumor-specific ligands displayed on exosome *** negative charge reduces nonspecific binding to vital cells with negatively charged lipid-membrane due to the electrostatic repulsion,thus lowering the side-effect and *** this review,we focus on the uniqueness of RNA nanoparticles for exosome surface display of chemical ligands,small peptides or RNA aptamers,for specific cancer targeting to deliver anticancer therapeutics,highlighting recent advances in targeted delivery of siRNA and miRNA that overcomes the previous RNAi delivery *** understanding of exosome engineering with RNA nanotechnology promises efficient therapies for a wide range of cancer subtypes.

关键词： RNA interference RNA nanotechnology Endolysosome trapping Exosome engineering Targeted delivery Chemical drug delivery

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Clinical applications of the CRISPR/Cas9 genome-editing system: Delivery options and challenges in precision medicine

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Genes & Diseases 2024年第1期11卷 268-282页

作者： Mohadeseh Khoshandam Hossein Soltaninejad Marziyeh Mousazadeh Amir Ali Hamidieh Saman Hosseinkhani Department of Reproductive Biology Academic Center for EducationCultureand Research(ACECR)Qom BranchQom ***Iran National Institute of Genetic Engineering and Biotechnology(NIGEB) Tehran 14155-6463Iran Faculty of Interdisciplinary Science and Technology Tarbiat Modares UniversityTehran 14117-13116Iran Pediatric Cell and Gene Therapy research center GeneCell&Tissue Research InstituteTehran UniversityofMedical SciencesTehran14155-6559Iran Department of nanobiotechnology Faculty of Biological SciencesTarbiat Modares UniversityTehran 14117-13116Iran Department of Biochemistry Faculty of Biological SciencesTarbiat Modares UniversityTehran 14117-13116Iran

CRISPR/Cas9 is an effective gene editing tool with broad applications for the pre-vention or treatment of numerous *** depends on CRiSPR(clustered regularly inter-spaced short palindromic repeats)as a bacterial immune system and plays as a gene editing *** to the higher specificity and efficiency of CRISPR/Cas9 compared to other editing ap-proaches,it has been broadly investigated to treat numerous hereditary and acquired ill-nesses,including cancers,hemolytic diseases,immunodeficiency disorders,cardiovascular diseases,visual maladies,neurodegenerative conditions,and a few X-linked ***/Cas9 system has been used to treat cancers through a variety of approaches,with sta-ble gene editing ***,the applications and clinical trials of CRisPR/Cas9 in various illnesses are *** to its high precision and efficiency,CRISPR/Cas9 strategies may treat gene-related illnesses by deleting,inserting,modifying,or blocking the expression of specific *** most challenging barrier to the in vivo use of CRISPR/Cas9 like off-target effects will be *** use of transfection vehicles for CRISPR/Cas9,including viral vectors(such as an Adeno-associated virus(AAV),and the development of non-viral vectors is also considered.

关键词： Clinical trials CRISPR/Cas9 Gene therapy Non-viral vectors Viral vectors

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Construction of RNA nanotubes

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Nano research 2019年第8期12卷 1952-1958页

作者： Hui Li Shaoying Wang Zhouxiang Ji Congcong Xu Lyudmila S. Shlyakhtenko Peixuan Guo center for RNA nanobiotechnology and Nanomedicine Division of Pharmaceutics and Pharmaceutical ChemistryCollege of Pharmacy/Department of Physiology & Cell BiologyCollege of Medicine/Dorothy M. Davis Heart and Lung Research Institute and James Comprehensive Cancer CenterThe Ohio State UniversityColumbusOH43210USA UNMC Nanoimaging Core Facility Department of Pharmaceutical SciencesCollege of Pharmacy University of Nebraska Medical CenterOmahaNE68182USA Present address: University of California San FranciscoCA94158USA Present address: P&Z Biological Technology NewarkNJ07103USA

Nanotubes are miniature materials with significant potential applications in nanotechnological, medical, biological and material sciences. The quest for manufacturing methods of nano-mechanical modules is in progress. For example, the application of carbon nanotubes has been extensively investigated due to the precise width control, but the precise length control remains challenging. Here we report two approaches for the one-pot self-assembly of RNA nanotubes. For the first approach, six RNA strands were used to assemble the nanotube by forming a 11 nm long hollow channel with the inner diameter of 1.7 nm and the outside diameter of 6.3 nm. For the second approach, six RNA strands were designed to hybridize with their neighboring strands by complementary base pairing and formed a nanotube with a six-helix hollow channel similar to the nanotube assembled by the first approach. The fabricated RNA nanotubes were characterized by gel electrophoresis and atomic force microscopy (AFM), confirming the formation of nanotube-shaped RNA nanostructures. Cholesterol molecules were introduced into RNA nanotubes to facilitate their incorporation into lipid bilayer. Incubation of RNA nanotube complex with the free-standing lipid bilayer membrane under applied voltage led to discrete current signatures. Addition of peptides into the sensing chamber revealed discrete steps of current blockage. Polyarginine peptides with different lengths can be detected by current signatures, suggesting that the RNA-cholesterol complex holds the promise of achieving single molecule sensing of peptides.

关键词： RNA nanotechnology peptide sensing RNA nanotube nanobiotechnology

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Disease modifying treatment of spinal cord injury with directly reprogrammed neural precursor cells in non-human primates

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World Journal of Stem Cells 2021年第5期13卷 452-469页

作者： Vladimir P Baklaushev Oleg V Durov Vladimir A Kalsin Eugene V Gulaev Sergey V Kim Ilya L Gubskiy Veronika A Revkova Ekaterina M Samoilova Pavel A Melnikov Dzhina D Karal-Ogly Sergey V Orlov Alexander V Troitskiy Vladimir P Chekhonin Alexander V Averyanov Jan-Eric Ahlfors Biomedical research Federal Research and Clinical Center of Specialized Medical Care and Medical TechnologiesFMBA of RussiaMoscow 115682MoskvaRussia Department of Neurosurgery Federal Research and Clinical Center of Specialized Medical Care and Medical TechnologiesFMBAMoscow 115682MoskvaRussia Department of Anesthesiology N.N.Blokhin Russian Cancer Research CentreMoscow 115478MoskvaRussia Radiology and Clinical Physiology Scientific research center Federal center of brain research and neurotechnologies of the Federal Medical Biological AgencyMoscow 117997Russia Department of Neurobiology Serbsky National Medical Research Center for Psychiatry and NarcologyMoscow 119992MoskvaRussia Department of Primatology Russian Acad Med SciResearch Institute of Medical PrimatologySochi 119992SochiRussia Department of Vascular Surgery Federal Research and Clinical Center of Specialized Medical Care and Medical TechnologiesFMBA of RussiaMoscow 115682MoskvaRussia Department of Basic and Applied Neurobiology V.P.Serbsky Federal Medical Research Center for Psychiatry and NarcologyRussia Department of Medical nanobiotechnology Pirogov Russian National Research Medical University(RNRMU)Moscow 115682MoskvaRussia New World Laboratories Laval H7V 4A7QuebecCanada

BACKGROUND The development of regenerative therapy for human spinal cord injury(SCI)is dramatically restricted by two main challenges:the need for a safe source of functionally active and reproducible neural stem cells and the need of adequate animal models for preclinical *** reprogramming of somatic cells into neuronal and glial precursors might be a promising solution to the first *** use of non-human primates for preclinical studies exploring new treatment paradigms in SCI results in data with more translational relevance to human *** To investigate the safety and efficacy of intraspinal transplantation of directly reprogrammed neural precursor cells(drNPCs).METHODS Seven non-human primates with verified complete thoracic SCI were divided into two groups:drNPC group(n=4)was subjected to intraspinal transplantation of 5 million drNPCs rostral and caudal to the lesion site 2 wk post injury,and lesion control(n=3)was injected identically with the equivalent volume of *** Follow-up for 12 wk revealed that animals in the drNPC group demonstrated a significant recovery of the paralyzed hindlimb as well as recovery of somatosensory evoked potential and motor evoked potential of injured *** resonance diffusion tensor imaging data confirmed the intraspinal transplantation of drNPCs did not adversely affect the morphology of the central nervous system or cerebrospinal fluid *** immunohistochemical analysis showed that drNPCs maintained SOX2 expression characteristic of multipotency in the transplanted spinal cord for at least 12 wk,migrating to areas of axon growth *** Our data demonstrated that drNPC transplantation was safe and contributed to improvement of spinal cord function after acute SCI,based on neurological status assessment and neurophysiological recovery within 12 wk after *** functional improvement described was not associated with neuronal differentiation of th

关键词： Direct cell reprogramming Neural precursor cells Directly reprogrammed neural precursor cells Spinal cord injury Nonhuman primates Regenerative therapy,Evoked potentials

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Biocompatible custom ceria nanoparticles against reactive oxygen species resolve acute inflammatory reaction after intracerebral hemorrhage

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Nano research 2017年第8期10卷 2743-2760页

作者： Dong-Wan Kang Chi Kyung Kim Han-Gil Jeong Min Soh Taeho Kim In-Young Choi SeuI-Ki Ki Do Yeon Kim Wookjin Yang Taeghwan Hyeon Seung-Hoon Lee Laboratory of Innovative nanobiotechnology Biomedical Research Institute Seoul National University Hospital Seoul 03080 Republic of Korea Department of Neurology Seoul National University Hospital Seou103080 Republic of Korea Department of Neurology Korea University Guro Hospital and Korea University College of Medicine Seou108308 Republic of Korea center for Nanoparticle research Institute for Basic Science (IBS) Seoul 151-742 Republic of Korea School of Chemical and Biological Engineering and Institute of Chemical Processes Seoul National University Seoul 151-742 Republic of Korea

Intracerebral hemorrhage （ICH） is a devastating subtype of stroke with a high mortality rate, for which there currently is no effective treatment. A perihematomal edema caused by an intense inflammatory reaction is more deleterious than the hematoma itself and can result in neurological deterioration and death. Ceria nanoparticles （CeNPs） are potent free radical scavengers with potential for biomedical applications. As oxidative stress plays a major role in post-ICH inflammation, we hypothesized that CeNPs might protect against ICH. To test this hypothesis, core CeNPs were synthesized using a modified reverse micelle method and covered with phospholipid-polyethylene glycol （PEG） to achieve biocompatibility. We investigated whether our custom-made biocompatible CeNPs have protective effects against ICH. The CeNPs reduced oxidative stress, hemin-induced cytotoxicity, and inflammation in vitro. In a rodent ICH model, intravenously administered CeNPs were mainly distributed in the hemorrhagic hemisphere, suggesting that they could diffuse through the damaged blood-brain barrier. Moreover, CeNPs attenuated microglia/macrophage recruitment around the hemorrhagic lesion and inflammatory protein expression. Finally, CeNP treatment reduced the brain edema by 68.4% as compared to the control. These results reveal the great potential of CeNPs as a novel therapeutic agent for patients with ICH.

关键词： ceria nanoparticles intracerebral hemorrhage free radical injury anti-inflammation neuroprotective agents biomedical application

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Hybrid lipopolymer vesicle drug delivery and release systems

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The Journal of Biomedical research 2021年第4期35卷 301-309页

作者： Erik Reimhult Mudassar Mumtaz Virk Department of nanobiotechnology Institute for Biologically Inspired MaterialsUniversity of Natural Resources and Life SciencesVienna1190 ViennaAustria.

Hybrid lipopolymer vesicles are membrane vesicles that can be self-assembled on both the micro-and *** the nanoscale,they are potential novel smart materials for drug delivery systems that could combine the relative strengths of liposome and polymersome drug delivery systems without their respective ***,little is known about their properties and how they could be ***,most methods of investigation are limited to the *** we provide a brief review on hybrid vesicle systems with a specific focus on recent developments demonstrating that nanoscale hybrid vesicles have different properties from their macroscale counterparts.

关键词： hybrid vesicles lipopolymersomes polymersomes drug delivery lipid domains triggered release

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New insight into surface properties of LB film of an amphiphilic terpolymer

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Chinese Chemical Letters 2011年第2期22卷 241-244页

作者： Xiu Li Yue Fang Ma, Zhi Fei Dai Meng Liu Lei Xing Hai Yan Fan Zhan Wen Xing Shao Qin Liu nanobiotechnology Division State Key Laboratory of Urban Water Resources and Environment School of Sciences Harbin Institute of Technology Harbin 150001 China Received 4 June 2010

Amphiphilic terpolymer（TPR） exhibits good film-forming behavior on pure water observed by means ofπ-A *** gain insight into physical properties of TPR,the films have been deposited on silicon substrates at different surface pressure by Langmuir-Blodgett（LB） *** was found that the increase in peak intensities of stretching mode was due to orderly packing of the *** contact angles increased with increasing surface pressure,indicating an increase in hydrophobicity due to dense packing of chains of *** cyclic voltammetric measurements indicated that TPR showed good current shielding effect for *** a word,LB films of TPR can produce a variety of structures with varied topography,enabling us to control not only the functionality of the surface,but also the interfacial transport characteristics.

关键词： Terpolymer Monolayer Langmuir-Blodgett film Surface

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Pattern of Growth and Dry Matter Accumulation in Some Improved Cowpea Varieties (<i>Vigna unguiculata</i>) Exposed to Alpha Nano Spin

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Advances in Nanoparticles 2021年第2期10卷 51-65页

作者： Hauwa Ahmad Kana Emmanuel Enock Goler Peter Barka Mshemlbula nanobiotechnology research Group Department of Botany Faculty of Science Federal University of Lafia Lafia Nigeria

Ten (10) cowpea varieties exposed to alpha nano spin were evaluated during the 2019 cropping season to access the role of alpha nano spin in their growth and dry matter accumulation at the Botanical garden of Federal University, Lafia. A Randomized Complete Block Design (RCBD) with four replications was used. The fourth replication was used for the destructive sampling over time. The seed was exposed to alpha nano spin before planting at 0, 20, 40 and 60 minutes respectively. Results of the study showed that the varieties differed significantly with respect to morphological traits studied (P s such as vine length, number of leaves and above ground stems were significantly influenced by alpha nano spin bombardment. 40 mins alpha nano spin resulted in maximum accumulation of dry matter, leaf area and leaf area index. The traits evaluated were stable under alpha nano spin exposure, suggesting that they could be useful indices in creating genetic variability in each of the varieties.

关键词： Alpha Nano Spin Cowpea Varieties Growth and Dry Matter Accumulation

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