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Cadherin-18 loss in prospermatogonia and spermatogonial stem cells enhances cell adhesion through a compensatory mechanism

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ZOOLOGICAL RESEARCH 2024年第5期45卷 1048-1060页

作者： Li, Xiao-Xiao Zhang, Dan-Chen Wang, Yan Wen, Jian Wang, Xing-Ju Cao, Yu-Lu Jiang, Ru Li, Jia-Rui Li, Yi-Nuo Liu, He-He Xie, Wen-Hai Xu, Zheng-Feng Hu, Ping Zou, Kang nanjing Agr univ Coll Anim Sci & Technol Germline Stem Cells & Microenvironm Lab Nanjing 210095 Jiangsu Peoples R China nanjing Agr univ Stem Cell Res & Translat Ctr Nanjing 210095 Jiangsu Peoples R China Shandong univ Technol Sch Life Sci & Med Zibo 255000 Shandong Peoples R China nanjing med univ Womens Hosp Nanjing Matern & Child Hlth Care Hosp Dept Prenatal Diag Nanjing 210004 Jiangsu Peoples R China

Extracellular membrane proteins are crucial for mediating cell attachment, recognition, and signal transduction in the testicular microenvironment, particularly germline stem cells. Cadherin 18 (CDH18), a type II classical cadherin, is primarily expressed in the nervous and reproductive systems. Here, we investigated the expression of CDH18 in neonatal porcine prospermatogonia (ProSGs) and murine spermatogonial stem cells (SSCs). Disruption of CDH18 expression did not adversely affect cell morphology, proliferation, self-renewal, or differentiation in cultured porcine ProSGs, but enhanced cell adhesion and prolonged cell maintenance. Transcriptomic analysis indicated that the down-regulation of CDH18 in ProSGs significantly up-regulated genes and signaling pathways associated with cell adhesion. To further elucidate the function of CDH18 in germ cells, Cdh18 knockout mice were generated, which exhibited normal testicular morphology, histology, and spermatogenesis. Transcriptomic analysis showed increased expression of genes associated with adhesion, consistent with the observations in porcine ProSGs. The interaction of CDH18 with beta-catenin and JAK2 in both porcine ProSGs and murine SSCs suggested an inhibitory effect on the canonical Wnt and JAK-STAT signaling pathways during CDH18 deficiency. Collectively, these findings highlight the crucial role of CDH18 in regulating cell adhesion in porcine ProSGs and mouse SSCs. Understanding this regulatory mechanism provides significant insights into the testicular niche.

关键词： Extracellular signal Fertility Quiescency Wnt signaling pathway IN-VITRO CULTURE SELF-RENEWAL PROPAGATION FAMILY LINE TRANSPLANTATION IDENTIFICATION MORPHOGENESIS ASSOCIATION SOMITE

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Homozygous CCDC146 mutation causes oligoasthenoteratozoospermia in humans and mice

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ZOOLOGICAL RESEARCH 2024年第5期45卷 1073-1087页

作者： Ye, Jing-Wei Abbas, Tanveer Zhou, Jian-Teng Chen, Jing Yang, Meng-Lei Huang, Xiong-Heng Zhang, Huan Ma, Hui Ma, Ao Xu, Bo Murtaza, Ghulam Shi, Qing-Hua Shi, Bao-Lu univ Sci & Technol China Affiliated Hosp 1Univ Sci & Technol ChinaSch Bas Inst Hlth & MedHefei Natl Lab Phys Sci Microscale Hefei Comprehens Natl Sci CtrDiv Reprod & Genet Hefei 230026 Anhui Peoples R China nanjing univ med sch Nanjing Drum Tower Hosp Inst Androl Affiliated Hosp Nanjing 210008 Jiangsu Peoples R China

Infertility represents a significant health concern, with sperm quantity and quality being crucial determinants of male fertility. Oligoasthenoteratozoospermia (OAT) is characterized by reduced sperm motility, lower sperm concentration, and morphological abnormalities in sperm heads and flagella. Although variants in several genes have been implicated in OAT, its genetic etiologies and pathogenetic mechanisms remain inadequately understood. In this study, we identified a homozygous nonsense mutation (c.916C>T, ***306*) in the coiled- coil domain containing 146 ( CCDC146 ) gene in an infertile male patient with OAT. This mutation resulted in the production of a truncated CCDC146 protein (amino acids 1-305), retaining only two out of five coiled-coil domains. To validate the pathogenicity of the CCDC146 mutation, we generated a mouse model ( Ccdc146( mut/mut) ) with a similar mutation to that of the patient. Consistently, the Ccdc146(mut/mut) mice exhibited infertility, characterized by significantly reduced sperm counts, diminished motility, and multiple defects in sperm heads and flagella. Furthermore, the levels of axonemal proteins, including DNAH17, DNAH1, and SPAG6, were significantly reduced in the sperm of Ccdc146(mut/mut) mice. Additionally, both human and mouse CCDC146 interacted with intraflagellar transport protein 20 (IFT20), but this interaction was lost in the mutated versions, leading to the degradation of IFT20. This study identified a novel deleterious homozygous nonsense mutation in CCDC146 that causes male infertility, potentially by disrupting axonemal protein transportation. These findings offer valuable insights for genetic counseling and understanding the mechanisms underlying CCDC146 mutant-associated infertility in human males.

关键词： Human infertility Sperm flagellum Oligoasthenoteratozoospermia CCDC146 IFT20 Intraflagellar transport MULTIPLE MORPHOLOGICAL ABNORMALITIES INTRAFLAGELLAR TRANSPORT MALE-FERTILITY PROTEOMIC ANALYSIS SPERMIOGENESIS IFT140

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Femtosecond laser-induced nanoparticle implantation into flexible substrate for sensitive and reusable microfluidics SERS detection

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INTERNATIONAL JOURNAL OF EXTREME MANUFACTURING 2024年第4期6卷 262-274页

作者： Hu, Yongxiang Zhou, Yu Luo, Guohu Li, Dege Qu, Minni Shanghai Jiao Tong univ Sch Mech Engn State Key Lab Mech Syst & Vibrat Shanghai 200240 Peoples R China nanjing Tech univ Sch Mech & Power Engn Nanjing 211816 Peoples R China Shanghai Jiao Tong univ Ctr Adv Elect Mat & Devices Sch Elect Informat & Elect Engn Shanghai 200240 Peoples R China

Surface-enhanced Raman spectroscopy (SERS) microfluidic system, which enables rapid detection of chemical and biological analytes, offers an effective platform to monitor various food contaminants and disease diagnoses. The efficacy of SERS microfluidic systems is greatly dependent on the sensitivity and reusability of SERS detection substrates to ensure repeated use for prolonged periods. This study proposed a novel process of femtosecond laser nanoparticle array (NPA) implantation to achieve homogeneous forward transfer of gold NPA on a flexible polymer film and accurately integrated it within microfluidic chips for SERS detection. The implanted Au-NPA strips show a remarkable electromagnetic field enhancement with the factor of 9 x 108 during SERS detection of malachite green (MG) solution, achieving a detection limit lower than 10 ppt, far better than most laser-prepared SERS substrates. Furthermore, Au-NPA strips show excellent reusability after several physical and chemical cleaning, because of the robust embedment of laser-implanted NPA in flexible substrates. To demonstrate the performance of Au-NPA, a SERS microfluidic system is built to monitor the online oxidation reaction between MG/NaClO reactants, which helps infer the reaction path. The proposed method of nanoparticle implantation is more effective than the direct laser structuring technique. It provides better performance for SERS detection, robustness of detection, and substrate flexibility and has a wider range of applications for microfluidic systems without any negative impact. Nanoparticle arrays with high accuracy and uniformity are implanted on flexible substrate. Prepared substrates achieve a detection limit lower than 10 ppt for SERS detection. SERS substrates represent excellent reusability and sensitivity in continuous detection. Nanoparticle arrays are integrated into microfluidic chip to monitor redox reaction.

关键词： femtosecond laser-induced transfer nanoparticle array surface-enhanced Raman spectrum microfluidic system SURFACE-ENHANCED RAMAN PDMS OPTIMIZATION SENSOR

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Prognostic stratification of baseline PET metabolic parameters combined with Bcl-2/c-Myc dual expression in patients with primary gastrointestinal diffuse large B-cell lymphoma

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China medical Abstracts(Internal medicine) 2024年第1期41卷 45-46页

作者：蒋冲 Dept Nucl med Nanjing Drum Tower HospNanjing Univ Med SchNanjing 210008

Objective To explore whether baseline PET metabolic parameters combined with B-cell lymphoma-2(Bcl-2)/cellular-myelocytomatosis viral oncogene (cMyc) dual expression (DE) can improve the prognostic stratification of p... 详细信息

关键词： lymphoma patients gastrointestinal

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Distributed fiber optic sensors for tunnel monitoring: A state-of-the-art review

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JOURNAL OF ROCK MECHANICS AND GEOTECHNICAL ENGINEERING 2024年第9期16卷 3841-3863页

作者： Zhang, Xuehui Zhu, Honghu Jiang, Xi Broere, Wout Delft univ Technol Dept Geosci & Engn Geoengn Sect NL-2628 CN Delft Netherlands nanjing univ Sch Earth Sci & Engn Nanjing 210023 Peoples R China Hong Kong Polytech univ Dept Civil & Environm Engn Kowloon Hong Kong Peoples R China

Distributed fiber optic sensors (DFOSs) possess the capability to measure strain and temperature variations over long distances, demonstrating outstanding potential for monitoring underground infrastructure. This study presents a state-of-the-art review of the DFOS applications for monitoring and assessing the deformation behavior of typical tunnel infrastructure, including bored tunnels, conventional tunnels, as well as immersed and cut-and-cover tunnels. DFOS systems based on Brillouin and Rayleigh scattering principles are both considered. When implementing DFOS monitoring, the fiber optic cable can be primarily installed along transverse and longitudinal directions to (1) measure distributed strains by continuously adhering the fiber to the structure's surface or embedding it in the lining, or (2) measure point displacements by spot-anchoring it on the lining surface. There are four critical aspects of DFOS monitoring, including proper selection of the sensing fiber, selection of the measuring principle for the specific application, design of an effective sensor layout, and establishment of robust field sensor instrumentation. These four issues are comprehensively discussed, and practical suggestions are provided for the implementation of DFOS in tunnel infrastructure monitoring.

关键词： Distributed fiber optic sensor (DFOS) Tunnel infrastructure Distributed strain sensing Point displacement monitoring Field instrumentation STRAIN SYSTEM

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Efficient stabilization of dredged sludge with high water content using an improved bio-carbonation of reactive magnesia cement method

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JOURNAL OF ROCK MECHANICS AND GEOTECHNICAL ENGINEERING 2024年第9期16卷 3760-3771页

作者： Wang, Rui Tang, Chaosheng Pan, Xiaohua Wang, Dianlong Dong, Zhihao Zhang, Xiying Lu, Xiancai nanjing univ Sch Earth Sci & Engn Nanjing 210023 Peoples R China Qinghai Inst Salt Lakes Chinese Acad Sci Qinghai Prov Key Lab Geol & Environm Salt Lake Xining 810008 Peoples R China

This study proposed an improved bio-carbonation of reactive magnesia cement (RMC) method for dredged sludge stabilization using the urea pre-hydrolysis strategy. Based on unconfined compression strength (UCS), pickling-drainage, and scanning electron microscopy (SEM) tests, the effects of prehydrolysis duration (T), urease activity (UA) and curing age (CA) on the mechanical properties and microstructural characteristics of bio-carbonized samples were systematically investigated and analyzed. The results demonstrated that the proposed method could significantly enhance urea hydrolysis and RMC bio-carbonation to achieve efficient stabilization of dredged sludge with 80% high water content. A significant strength increment of up to about 1063.36 kPa was obtained for the bio-carbonized samples after just 7 d of curing, which was 2.64 times higher than that of the 28-day cured ordinary Portland cement-reinforced samples. Both elevated T and UA could notably increase urea utilization ratio and carbonate ion yield, but the resulting surge in supersaturation also affected the precipitation patterns of hydrated magnesia carbonates (HMCs), which weakened the cementation effect of HMCs on soil particles and further inhibited strength enhancement of bio-carbonized samples. The optimum formula was determined to be the case of T = 24 h and UA = 10 U/mL for dredged sludge stabilization. A 7-day CA was enough for bio-carbonized samples to obtain stable strength, albeit slightly affected by UA. The benefits of high efficiency and water stability presented the potential of this method in achieving dredged sludge stabilization and resource utilization. This investigation provides informative ideas and valuable insights on implementing advanced bio-geotechnical techniques to achieve efficient stabilization of soft soil, such as dredged sludge. (c) 2024 Institute of Rock and Soil Mechanics, Chinese Academy of Sciences. Production and hosting by Elsevier B.V. This is an open access artic

关键词： Reactive magnesia cement (RMC) bio-carbonation Urea pre-hydrolysis Dredged sludge Efficient stabilization Unconfined compressive strength Microbially induced carbonate precipitation (MICP) FLY-ASH STRENGTH MGO MICROSTRUCTURE PRECIPITATION UREA

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Traceable Lactate-Fueled Self-Acting Photodynamic Therapy against Triple-Negative Breast Cancer

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RESEARCH 2024年第1期7卷 723-733页

作者： Zhang, Yifan Feng, Guangle He, Ting Yang, Min Lin, Jing Huang, Peng Shenzhen univ Int Canc Ctr Sch Biomed Engn Med SchLab Evolutionary Theranost LETMarshall La Shenzhen 518060 Peoples R China

The depth of light penetration and tumor hypoxia restrict the efficacy of photodynamic therapy (PDT) in triple-negative breast cancer (TNBC), while the overproduction of lactate (LA) facilitates the development, aggressiveness, and therapy resistance of TNBC. To address these issues, a self-acting PDT nanosystem (HL@hMnO2-LOx@HA) is fabricated by loading 2-(1-hexyloxyethyl)-2-devinyl pyropheophorbide-alpha (HPPH), luminol, and LA oxidase (LOx) in a hyaluronic acid (HA)-coated hollow manganese dioxide (hMnO2) nanoparticle. LOx catalyzes the oxidation of LA into pyruvate and hydrogen peroxide (H2O2), thus depleting the overproduced intratumoral LA. In the acidic tumor microenvironment, H2O2 reacts with luminol and hMnO2 to yield blue luminescence as well as O2 and Mn2+, respectively. Mn2+ could further enhance this chemiluminescence. HPPH is then excited by the chemiluminescence through chemiluminescence resonance energy transfer for self-illuminated PDT. The generated O2 alleviates the hypoxia state of the TNBC tumor to produce sufficient 1O2 for self-oxygenation PDT. The Mn2+ performs T1 magnetic resonance imaging to trace the self-acting PDT process. This work provides a biocompatible strategy to conquer the limits of light penetration and tumor hypoxia on PDT against TNBC as well as LA overproduction.

关键词： TUMOR LUMINESCENCE HYPOXIA OXYGEN

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Effect of solution treatment on the microstructure, phase transformation behavior and functional properties of NiTiNb ternary shape memory alloys fabricated via laser powder bed fusion in-situ alloying

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INTERNATIONAL JOURNAL OF EXTREME MANUFACTURING 2024年第4期6卷 045002-045002页

作者： Xi, Rui Jiang, Hao Li, Guichuan Zhang, Zhihui Wei, Huiliang Zhao, Guoqun Van Humbeeck, Jan Wang, Xiebin Shandong univ Key Lab Liquid Solid Struct Evolut & Proc Mat Minist Educ Jingshi Rd 17923 Jinan 250061 Peoples R China univ Leuven Dept Mat Engn KU Leuven Kasteelpark Arenberg 44bus 2450 B-3001 Heverlee Belgium Jilin univ Key Lab Bion Engn Minist Educ Renmin St 5988 Changchun 130025 Peoples R China nanjing univ Sci & Technol Sch Mech Engn Xiaolingwei St 200 Nanjing 210094 Peoples R China

Post-heat treatment is commonly employed to improve the microstructural homogeneity and enhance the mechanical performances of the additively manufactured metallic materials. In this work, a ternary (NiTi)(91)Nb-9(at.%) shape memory alloy was produced by laser powder bedfusion (L-PBF) using pre-alloyed NiTi and elemental Nb powders. The effect of solution treatment on the microstructure, phase transformation behavior and mechanical/functional performances was investigated. Thein-situalloyed (NiTi)91Nb9alloy exhibits a submicroncellular-dendritic structure surrounding the supersaturated B2-NiTi matrix. Upon high-temperature (1273 K) solution treatment, Nb-rich precipitates were precipitated from the supersaturated matrix. The fragmentation and spheroidization of the NiTi/Nb eutectics occurred during solution treatment, leading to a morphological transition from mesh-like into rod-like and sphere-like. Coarsening of the beta-Nb phases occurred with increasing holding time. The martensite transformation temperature increases after solution treatment, mainly attributed to:(i) reduced lattice distortion due to the Nb expulsion from the supersaturated B2-NiTi, and (ii)the Ti expulsion from the beta-Nb phases that lowers the ratio Ni/Ti in the B2-NiTi matrix, which resulted from the microstructure changes from non-equilibrium to equilibrium state. The thermal hysteresis of the solutionized alloys is around 145 K after 20% pre-deformation, which is comparable to the conventional NiTiNb alloys. A short-term solution treatment (i.e. at 1273 K for 30 min) enhances the ductility and strength of the as-printed specimen, with the increase of fracture stress from (613 +/- 19) MPa to (781 +/- 20) MPa and the increase of fracture strain from (7.6 +/- 0.1)% to (9.5 +/- 0.4)%. Both the as-printed and solutionized samples exhibit good tensile shape memory effects with recovery rates>90%. This work suggests that post-process heat treatment is essential to optimize the microstructure and

关键词： shape memory alloy NiTiNb laser powder bed fusion in-situ alloying heat treatment MECHANICAL-PROPERTIES HEAT-TREATMENT WIDE HYSTERESIS TI-NB MARTENSITIC-TRANSFORMATION THERMOMECHANICAL RESPONSE ELECTRON-MICROSCOPY HIGH-STRENGTH EVOLUTION COMPOSITE

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In vivo 3-photon fluorescence microscopy of white matter in mouse brain excited at the 1700 nm window

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JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES 2024年第5期17卷 81-87页

作者： Huang, Jie Zhong, Jincheng Tong, Shen Zhang, Yingxian Qiu, Ping Wang, Ke Shenzhen univ Coll Phys & Optoelect Engn Key Lab Optoelect Devices & Syst Minist Educ & Guangdong Prov Shenzhen 518060 Peoples R China Xuzhou med univ Sch Med Informat & Engn Xuzhou 221004 Peoples R China

White matter, a densely packed collection of myelinated axons, plays an essential part in neural networks. With high spatial resolution and deep penetration, multi-photon microscopy (MPM) is promising for white matter imaging in animal models in vivo. The third harmonic generation (THG) signal can be generated from white matter, but the bottom part of the white matter layer generates weak THG due to its high scattering. Here, we demonstrate an in vivo labeling and imaging technology, capable of visualizing the white matter layer in the mouse brain, combining fluorescence labeling with MitoTracker Red and three-photon fluorescence (3PF) microscopy excited at the 1700 nm window. 3PF signals are several times higher than THG signals, resulting in deeper imaging of the white matter layer with the former. Our results indicate that 3PF microscopy is a promising technology for white matter imaging in the deep brain in vivo.

关键词： Three-photon microscopy white matter myelin MitoTracker Red DEEP NEURONS MYELIN

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Polar-coordinate line-projection light-curing continuous 3D printing for tubular structures

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INTERNATIONAL JOURNAL OF EXTREME MANUFACTURING 2024年第4期6卷 247-260页

作者： Wang, Huiyuan Liu, Siqin Yin, Xincheng Huang, Mingming Fu, Yanzhe Chen, Xun Wang, Chao Sun, Jingyong Yan, Xin Han, Jianmin Yang, Jiping Wang, Zhijian Wang, Lizhen Fan, Yubo Li, Jiebo Beihang univ Inst Med Photon Beijing Adv Innovat Ctr Biomed Engn Key Lab Biomech & MechanobiolMinist EducSch Biol Beijing 100191 Peoples R China Beihang univ Sch Mech Engn & Automat Beijing 100191 Peoples R China Peking univ sch & Hosp Stomatol Beijing 100081 Peoples R China Beihang univ Sch Mat Sci & Engn Beijing 100191 Peoples R China

3D printing techniques offer an effective method in fabricating complex radially multi-material structures. However, it is challenging for complex and delicate radially multi-material model geometries without supporting structures, such as tissue vessels and tubular graft, among others. In this work, we tackle these challenges by developing a polar digital light processing technique which uses a rod as the printing platform. The 3D model fabrication is accomplished through line projection. The rotation and translation of the rod are synchronized to project and illuminate the photosensitive material volume. By controlling the distance between the rod and the printing window, we achieved the printing of tubular structures with a minimum wall thickness as thin as 50 micrometers. By controlling the width of fine slits at the printing window, we achieved the printing of structures with a minimum feature size of 10 micrometers. Our process accomplished the fabrication of thin-walled tubular graft structure with a thickness of only 100 micrometers and lengths of several centimeters within a timeframe of just 100 s. Additionally, it enables the printing of axial multi-material structures, thereby achieving adjustable mechanical strength. This method is conducive to rapid customization of tubular grafts and the manufacturing of tubular components in fields such as dentistry, aerospace, and more. Polar-coordinate-based line projection enables rapid printing of tubular structures with higher precision and compressive strength. Using a cylindrical auxiliary axis allows for the manufacture of radial multi-material structures and facilitates printing of coaxial structures. It is beneficial for the rapid customization of tubular implants and for manufacturing tubular components in dentistry, aerospace, and other fields.

关键词： 3D printing polar coordinate line projection light-curing tubular structure radially multi-material structures PROCESS PARAMETERS STENT FABRICATION

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