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Methylprednisolone inhibits activated CD4^+ T cell survival promoted by toll-like receptor ligands

Hepatobiliary & Pancreatic Diseases International 2010年第4期9卷 376-383页

作者： Lu, You-Sheng Pu, Li-Yong Li, Xiang-Cheng Wang, Xue-Hao nanjing med Univ Affiliated Hosp 1 Liver Transplantat Ctr Peoples Hosp Jiangsu Prov Nanjing 210029 Peoples R China

BACKGROUND: Methylprednisolone (MP) can affect the survival of CD4(+) T lymphocytes and plays an important role in adaptive immune responses; however, its mechanism of action is not clear. Recent studies have shown that toll-like receptors (TLRs) on CD4(+) T cells can directly modulate adaptive immune responses by affecting the survival and proliferation of activated CD4(+) T cells. This study aimed to investigate the relationship between MP, TLRs and activated CD4(+) T cells. METHODS: We separated and purified CD4(+) T cells from mice, activated them in vitro, and co-cultured them with TLR ligands, MP or inhibitors of nuclear factor-kappa B (NF-kappa B) and activator protein 1 (AP-1). We then assessed CD4(+) T cell survival and proliferation and the expression of NF-kappa B and AP-1. RESULTS: Activated CD4(+) T cells showed increased TLR-3 and TLR-9 mRNA expression, but polyinosinic-polycytidylic acid (poly I:C) and MP had no effect on the expression of these mRNAs. Still, poly I:C and CpG oligodeoxynucleotides (CpG DNA) increased the survival of activated CD4(+) T cells, whereas MP reduced the survival of activated CD4(+) T cells and could inhibit the survival effects of poly I:C and CpG DNA. The NF-kappa B essential modifier-binding domain (NBD) inhibited the survival of activated CD4(+) T cells induced by poly I:C and CpG DNA, but the AP-1 inhibitor crucumin did not have the same effect. The increased expression of NF-kappa B induced by poly I:C and CpG DNA in activated CD4(+) T cells could be inhibited by MP, but the same was not true for the increased expression of AP-1 induced by poly I:C and CpG DNA. Finally, the proliferation of activated CD4(+) T cells was not affected by poly I:C or MP. CONCLUSION: The survival of activated CD4(+) T cells is promoted by TLR ligands, but this effect is inhibited by MP.

关键词： methylprednisolone toll-like receptor CD4(+) T lymphocytes NF-kappa B

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THE ROLE OF IGF-1 GENE EXPRESSION ABNORMALITY IN PATHOGENESIS OF DIABETIC PERIPHERAL NEUROPATHY

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Chinese medical Sciences Journal 2002年第4期17卷 204-209页

作者：李剑波汪承亚陈家伟李晓璐冯振卿马洪太 Department of Endocrinology First Aff. Hosp. Nanjing Med. Univ. Nanjing 210029 China

Objective. To explore the role of insulin-like growth factor 1 (IGF-1)gene expression abnormality in neurotrophic causes of diabetic peripheral ***. Diabetes was induced in Sprague Dawley rats by alloxan. The parameters were measured as follows: IGF-1 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR); IGF-1 peptide by enzyme-linked immunosorbent assay (ELISA); electrophysiological parameters of nerves by evoked electromyogram; morphometric evaluation of sciatic nerves under light microscope and transmission electron ***. During early diabetic stage, IGF-1 mRNA [(0.430±0.031)vs. (0.370±0.016), P ＜0.01,(0.430 ± 0.031 ) vs. (0.280 ± 0.010) , P ＜0.001, respectively], IGF - 1 peptide contents [ (38.44 ± 3.60)ng/mgvs. (30.06±2.41) ng/mg, P ＜0.01, (38.44±3.6) ng/mgvs. (3.71 +2.70) ng/mg, P ＜0.001,respectively] in sciatic nerve tissue reduced in diabetic rats with hyperglycemia and varied with severity of diabetic state when compared with non-diabetic control rats, and further gradually down-regulated in the diabetic rats with duration of diabetes [IGF-1 mRNA (0. 320 ± 0. 021) ～ (0. 230 + 0. 060); IGF-1 peptide (28.80 ± 3.30) ～(19. 51 + 1.80)ng/mg]. Furthermore, they correlated with nerve functional (sensory nerve conduction velocity:r = 0. 741, P ＜0. 001; amplitude ofevokedpotential: r = 0. 716, P ＜0. 001, respectively)andstructuralabnormality (axonal areas r = 0. 81, P ＜ 0. 001 ) of sciatic nerve. No difference was found in the above parameters between diabetic rats with euglycemia and non-diabetic control ***. IGF-1 gene expression in tissues was down-regulated from early diabetic stage, and varied with the severity and duration of diabetic state. The decrement in IGF-1 level might contribute to the initiation and development of diabetic neuropathy via autocrine or paracrine pathway.

关键词： IGF-1 gene expression diabetic peripheral neuropathy rat

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Association between IRS-2 G1057D polymorphism and risk of gastric cancer

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World Journal of Gastrointestinal Oncology 2012年第1期4卷 9-15页

作者： Xiao-Mei Zhao Jie Chen Li Yang Xuan Luo Lin-Lin Xu Dong-Xiao Liu Su-Lan Zhai Ping Li Xue-Rong Wang nanjing med Univ Dept Pharmacol 140 Hanzhong Rd Nanjing 210029 Jiangsu Peoples R China Southern med Univ Affiliated Pingxiang Hosp Dept Pharm Pingxiang 337000 Jiangxi Peoples R China nanjing med Univ Affiliated Hosp 1 Dept Gen Surg Nanjing 210029 Jiangsu Peoples R China

AIM: To investigate the relationship between insulin receptor substrate-2 (IRS-2) G1057D polymorphism and the risk of gastric cancer (GC) in a Chinese population. METHODS: A case-control study with 197 GC patients and 156 age- and sex-matched control subjects was conducted. The genotypes of polymorphism were assessed by polymerase chain reaction-restriction fragment length polymorphism. RESULTS: The genotype frequencies of IRS-2 G1057D polymorphism in cases were obviously different from those in the control group (P = 0.031). Compared with GG genotype carriers, the risk for GC was significantly higher (adjusted odds ratio = 2.32, 95% CI: 1.03-5.23, P = 0.042) in the individuals with the IRS-2 DD geno-type. Furthermore, stratified analysis was performed based on age, sex, smoking status and residence, but no significant difference between the two groups was found. In addition, no significant association between genotypes and clinicopathological features was observed either. CONCLUSION: This study demonstrates that IRS-2 G1057D is involved in susceptibility to GC, although further large-sample studies are still needed.

关键词： Gastric cancer Insulin receptor substrate-2 Polymorphism Genotype Case-Control study

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Two new tetrahydronaphthalenes from the stem of Impatiens balsamina L.

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Chinese Chemical Letters 2010年第4期21卷 440-442页

作者： Chen, Xiu Mei Qian, Shi Hui Feng, Feng Jiangsu Prov Acad Tradit Chinese med Nanjing 210028 Peoples R China China Pharmaceut Univ Dept Phytochem Nanjing 210009 Peoples R China

Two new tetrahydronaphthalenes,lα,2α-diol-4α-ethoxy-1,2,3,4-tetrahydronaphthalene(1) and 1a,2a,4β-triol-1,2,3,4- tetrahydronaphthalene(2),were isolated from the stem of Impatiens balsamina *** structures were eluc... 详细信息

关键词： Impatiens balsamina L. Balsaminaceae Tetrahydronaphthalene

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A novel diarylheptanoid bearing flavonol moiety from the rhizomes of Alpinia officinarum Hance

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Chinese Chemical Letters 2010年第2期21卷 194-196页

作者： Zhao, Ling Liang, Jing Yu Zhang, Jing Yu Chen, Yun China Pharmaceut Univ Dept Nat Med Chem Nanjing 210009 Peoples R China

A novel diarylheptanoid bearing flavonol moiety,named officinin A(1),along with two known compounds galangin and kaempferide were isolated from the rhizomes of Alpina officinarum *** structure elucidation was accompli... 详细信息

关键词： Alpinia officinarum Hance Diarylheptanoid Officinin A

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A new diterpenoid from Euphorbia helioscopia

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Chinese Chemical Letters 2010年第1期21卷 73-75页

作者： Geng, Di Shi, Yao Min, Zhi Da Liang, Jing Yu China Pharmaceut Univ Dept Nat Med Chem Nanjing 210009 Peoples R China

A new jatrophane-type diterpenoid,7β,14β,15β-triacetoxy-3β-benzoyloxy-9-oxojatropha-5E,11E-diene,named euphornin N was isolated from Euphorbia helioscopia *** structure and relative stereochemistry of the new comp... 详细信息

关键词： 7β 14β 15β-Triacetoxy-3β-benzoyloxy-9-oxojatropha-5E 11E-diene Euphornin N Euphorbia helioscopia

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Expression of iNOS in early injury in a rat model of small-for-size liver transplantation

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Hepatobiliary & Pancreatic Diseases International 2009年第2期8卷 146-151页

作者： Jiang, Wei-Wei Kong, Lian-Bao Li, Guo-Qiang Wang, Xue-Hao nanjing med Univ Affiliated Hosp 1 Liver Transplantat Ctr Natl Inst Living Donor Liver Transplantat Nanjing 210029 Peoples R China

BACKGROUND: Living donor liver transplantation has been widely accepted as the treatment of choice for end-stage liver disease. Large amounts of nitric oxide generated by inducible nitric oxide synthase (iNOS) have been shown to play an important role in many inflammatory and immune reactions, but expression of iNOS in small-for-size liver transplantation is unknown. The aims of this study were to determine the time course of iNOS mRNA and protein as well as the redox state of liver biopsies in a rat model of small-for-size liver transplantation. METHODS: Male Sprague-Dawley rats were divided into a control group, a warm ischemia-reperfusion (IR) group, and a small-for-size liver graft group. Real-time RT-PCR and Western blotting were used to characterize the time course of the expression of iNOS mRNA and protein, respectively. Malondialdehyde (MDA) and superoxide dismutase (SOD) were used as markers to characterize the redox state of liver tissues, and the time courses of MDA and SOD levels were also measured. RESULTS: The expression of iNOS mRNA and protein levels in the warm IR and small-for-size graft groups both significantly increased after reperfusion, and peaked at 3 hours. Moreover, the increase in MDA was accompanied by increased iNOS in the period of 1-24 hours after reperfusion. The MDA levels in the warm IR and small-for-size graft groups significantly increased after reperfusion, peaked at 3 hours, and decreased thereafter. The direction of change in SOD was opposite that of the change in MDA. CONCLUSIONS: The expression of iNOS mRNA and protein is activated after reperfusion both in hepatic warm IR injury and small-for-size liver graft. Furthermore, the results of this study suggest that iNOS contributes to the damage in warm IR injury and small-for-size grafts via free oxygen radicals.

关键词： inducible nitric oxide synthase small-for-size graft ischemia-reperfusion injury liver transplantation

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Total synthesis of endothelin 1 by microwave-assisted solid phase method

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Chinese Chemical Letters 2010年第4期21卷 388-390页

作者： Qian, Hai Chen, Wei Zhou, Jin Pei Huang, Wen Long Zhang, Hui Bin Jin, Jing China Pharmaceut Univ Dept Med Chem Nanjing 210009 Peoples R China China Pharmaceut Univ Ctr Drug Discovery Nanjing 210009 Peoples R China

A microwave-assisted solid phase synthesis for endothelin 1 is *** endothelin 1 was synthesized efficiently on Wang resin under microwave irradiation using Fmoc/tBu orthogonal protection *** whole peptide was cleaved from the resin and two disulphide bridges were formed under air oxidation at room *** purity and efficiency of synthesizing the peptide is much higher than other methods used before.

关键词： Endothelin 1 Microwave irradiation Solid phase Disulphide bridge

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A convenient synthesis of ezetimibe analogs as cholesterol ab sorption inhibitors

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Chinese Chemical Letters 2010年第1期21卷 67-69页

作者： Ji, Jian Feng Zhang, Hui Bin Huang, Wen Long Qian, Hai Zhou, Jin Pei China Pharmaceut Univ Ctr Drug Discovery Nanjing 210009 Peoples R China China Pharmaceut Univ Dept Med Chem Nanjing 210009 Peoples R China

A convenient method for the synthesis of ezetimibe analogs as cholesterol absorption inhibitors was *** key step in the synthesis was the intramolecular ring formation through Mitsunobu ***,a new series of analogs was... 详细信息

关键词： Ezetimibe Synthesis Cholesterol absorption inhibitor

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Damage control surgery for severe pancreatic trauma

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Hepatobiliary & Pancreatic Diseases International 2007年第6期6卷 569-571页

作者： Wang, Ge-Fei Li, You-Sheng Li, Jie-Shou nanjing Univ Sch Med Jinling Hosp Res Inst Gen Surg Nanjing 210002 Peoples R China

Pancreatic trauma as a challenge before and during explorative laparotomy is associated with multiple visceral injuries or critical conditions. Its optimal management remains *** current concept of damage control surg... 详细信息

关键词： Damage control surgery for severe pancreatic trauma

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